Click here to switch to the map view.

The map label for this gene is dmlR [H]

Identifier: 34498758

GI number: 34498758

Start: 3597003

End: 3597899

Strand: Reverse

Name: dmlR [H]

Synonym: CV_3303

Alternate gene names: 34498758

Gene position: 3597899-3597003 (Counterclockwise)

Preceding gene: 34498762

Following gene: 34498757

Centisome position: 75.73

GC content: 70.46

Gene sequence:

>897_bases
ATGAGCGAGCTGAAGCAGCTGGAGACCTTCGTCGCCGTGGTGGGGCTGGGCAGCTTGTCGGCCGCGGCGCGGCAGCTGGG
CGTGGTGCCGGCCATGGTGGGGCGGCGCCTGGACGCGCTGGAGGAGCGGCTGGGGGTGCGCCTGTTGGTGCGCACCACCC
GCAGCGTGGCGCCGACCCAGGAAGGTGAGGCTTTTTATGAAGATTGCCAGCGGATACTGGCCGATCTGACTGAGGCGGAG
GCGGCGGTGGCGTCCGGCAGCGGCAGGGCGCGCGGCCATCTGCGCCTGTCGGCGCCGGCCGGCTTCGGCCGCCGCCATGT
GGCGCCGCACGTCGCCGCCTTTCAGCGCCTGCATCCCGAGGTCAAGGTGACCCTGGACCTGTCAGACCGGCTGGTGGACC
TGCAGCGCGACCGCATCGACTGCGCGATCCGCATCTCCGATCTGTCCGACTCCGGCCTGGTGGCGATCCGGCTGGCGGAA
AACCATCGCGTGGTGGTGGCGTCCCCGGCCTATCTGGCCGCCCACGGCGCGCCGCGCGCGCTGGAGGATCTGCAGCGCCA
TCAGTGCCTGTCGCTGGGCGAGAGCCAGAGCCGCGGCTGGAGCTTTGCGGTCGACGGCAAGCCGGTGAACCTGAAGGTGT
CGGGCGCGCTGGAGTGCAACGACGGCGCGGTGCTGCACGATTGGGCGCTGCAAGGCCTGGGCCTGGCCTGGCGCTCGCTG
TGGGAGGTCAAGGACGATCTGTCCGAAGGCCGGCTGGCGACGGTGCTCGACGATTTCTCGTCGCCGGACTACCCGGTGTA
CGCGGTGGTGCCGCAGCGCAAGCTGCTGCCGGCCAGGGTGCGGCATTTCATCGAACATCTGAAAGCCGCCTACGCCGCGC
CGGGCTATTGGGATTGA

Upstream 100 bases:

>100_bases
GACATGATGATGCTGCAATGCAATGTTTGACAGTCTATTCGCCGCGCGAGTAAAAAGAAATGGCATGAATTGCACAACAT
CTTTTACTTTTAGGTAGCTA

Downstream 100 bases:

>100_bases
GCGCGGCGCCGGTTTGCCGCGCCGGCCCCGGCTTGCTAAGGTAGGGCCTCGCCGCGCAGGGGCCGCGAGCACAAGAAGAC
AAACAACGTGAACAAATACC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSELKQLETFVAVVGLGSLSAAARQLGVVPAMVGRRLDALEERLGVRLLVRTTRSVAPTQEGEAFYEDCQRILADLTEAE
AAVASGSGRARGHLRLSAPAGFGRRHVAPHVAAFQRLHPEVKVTLDLSDRLVDLQRDRIDCAIRISDLSDSGLVAIRLAE
NHRVVVASPAYLAAHGAPRALEDLQRHQCLSLGESQSRGWSFAVDGKPVNLKVSGALECNDGAVLHDWALQGLGLAWRSL
WEVKDDLSEGRLATVLDDFSSPDYPVYAVVPQRKLLPARVRHFIEHLKAAYAAPGYWD

Sequences:

>Translated_298_residues
MSELKQLETFVAVVGLGSLSAAARQLGVVPAMVGRRLDALEERLGVRLLVRTTRSVAPTQEGEAFYEDCQRILADLTEAE
AAVASGSGRARGHLRLSAPAGFGRRHVAPHVAAFQRLHPEVKVTLDLSDRLVDLQRDRIDCAIRISDLSDSGLVAIRLAE
NHRVVVASPAYLAAHGAPRALEDLQRHQCLSLGESQSRGWSFAVDGKPVNLKVSGALECNDGAVLHDWALQGLGLAWRSL
WEVKDDLSEGRLATVLDDFSSPDYPVYAVVPQRKLLPARVRHFIEHLKAAYAAPGYWD
>Mature_297_residues
SELKQLETFVAVVGLGSLSAAARQLGVVPAMVGRRLDALEERLGVRLLVRTTRSVAPTQEGEAFYEDCQRILADLTEAEA
AVASGSGRARGHLRLSAPAGFGRRHVAPHVAAFQRLHPEVKVTLDLSDRLVDLQRDRIDCAIRISDLSDSGLVAIRLAEN
HRVVVASPAYLAAHGAPRALEDLQRHQCLSLGESQSRGWSFAVDGKPVNLKVSGALECNDGAVLHDWALQGLGLAWRSLW
EVKDDLSEGRLATVLDDFSSPDYPVYAVVPQRKLLPARVRHFIEHLKAAYAAPGYWD

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=291, Percent_Identity=38.4879725085911, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI1789440, Length=287, Percent_Identity=35.8885017421603, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1786401, Length=298, Percent_Identity=34.5637583892617, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1789639, Length=296, Percent_Identity=29.7297297297297, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=31.0344827586207, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787589, Length=291, Percent_Identity=29.8969072164948, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787128, Length=292, Percent_Identity=27.7397260273973, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789173, Length=267, Percent_Identity=26.9662921348315, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786448, Length=273, Percent_Identity=26.7399267399267, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1788706, Length=286, Percent_Identity=25.8741258741259, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790011, Length=300, Percent_Identity=24, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787601, Length=144, Percent_Identity=27.0833333333333, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI157672245, Length=175, Percent_Identity=29.7142857142857, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32501; Mature: 32370

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELKQLETFVAVVGLGSLSAAARQLGVVPAMVGRRLDALEERLGVRLLVRTTRSVAPTQ
CCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCEEEEEEHHHCCCCCC
EGEAFYEDCQRILADLTEAEAAVASGSGRARGHLRLSAPAGFGRRHVAPHVAAFQRLHPE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCC
VKVTLDLSDRLVDLQRDRIDCAIRISDLSDSGLVAIRLAENHRVVVASPAYLAAHGAPRA
EEEEEECCHHHHHHHCCCEEEEEEEECCCCCCEEEEEEECCCEEEEECCHHEECCCCCHH
LEDLQRHQCLSLGESQSRGWSFAVDGKPVNLKVSGALECNDGAVLHDWALQGLGLAWRSL
HHHHHHHHHHHCCCCCCCCEEEEECCEEEEEEEEEEEECCCCCEEHHHHHHHHHHHHHHH
WEVKDDLSEGRLATVLDDFSSPDYPVYAVVPQRKLLPARVRHFIEHLKAAYAAPGYWD
HHHHHHHCCCCEEEHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SELKQLETFVAVVGLGSLSAAARQLGVVPAMVGRRLDALEERLGVRLLVRTTRSVAPTQ
CHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCEEEEEEHHHCCCCCC
EGEAFYEDCQRILADLTEAEAAVASGSGRARGHLRLSAPAGFGRRHVAPHVAAFQRLHPE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCC
VKVTLDLSDRLVDLQRDRIDCAIRISDLSDSGLVAIRLAENHRVVVASPAYLAAHGAPRA
EEEEEECCHHHHHHHCCCEEEEEEEECCCCCCEEEEEEECCCEEEEECCHHEECCCCCHH
LEDLQRHQCLSLGESQSRGWSFAVDGKPVNLKVSGALECNDGAVLHDWALQGLGLAWRSL
HHHHHHHHHHHCCCCCCCCEEEEECCEEEEEEEEEEEECCCCCEEHHHHHHHHHHHHHHH
WEVKDDLSEGRLATVLDDFSSPDYPVYAVVPQRKLLPARVRHFIEHLKAAYAAPGYWD
HHHHHHHCCCCEEEHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]