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The map label for this gene is nth [H]

Identifier: 34498748

GI number: 34498748

Start: 3583852

End: 3584484

Strand: Direct

Name: nth [H]

Synonym: CV_3293

Alternate gene names: 34498748

Gene position: 3583852-3584484 (Clockwise)

Preceding gene: 34498747

Following gene: 34498749

Centisome position: 75.43

GC content: 65.88

Gene sequence:

>633_bases
GTGAACGCCGCCAAACGCCAAGAAATCTTCCGCCGCCTGCGCGACGACAATCCGCATCCGACCACCGAGCTGGAATACAA
CACGCCGTTCGAGCTGCTGATCTCGGTGCTGCTGTCGGCCCAGGCCACCGACGTCGGCGTCAACAAGGCCACCCGCCGCC
TGTACCCGGTGGCCAACACGCCGGCCGCGATGCTGGCGCTGGGCGAGGAAGGCCTGGCCGAATACATCAAGACCATAGGC
CTGTACAAGACCAAGGCCAGGAACGTGATCGCCACCTGCAGGCTGCTGCTGGAAAAACACGGCGGCGAGGTGCCGCAGAC
GCGCGAGGCGCTGGAGGCGCTGCCCGGCGTCGGCCGCAAGACCGCCAACGTGGTGCTCAACACCGCCTTCGGCCAGCCCA
CCATCGCGGTGGACACCCACATCTTCCGCGTGTCCAACCGCACCCGCATCGCGCCGGGCAAGGATGTGCGCGAGGTGGAG
GACAAGCTGGAGCGTTTCGTGCCCGCCGAATTCAAGCTGGACGCGCATCACTGGCTGATCCTGCTGGGGCGCTACGTATG
CAAGGCCAGGAAGCCGGAATGCCACCGCTGCGCGATCGCGGACCTCTGCGAATACCCGGCAAAACCGTTATGA

Upstream 100 bases:

>100_bases
CCGCCACCGCGTCCGCCGACAAGATGGCGGTGATACGCGCCGCGATGGAGCGCGCCGCCGCGATGAAGGCCGCCCAGCAG
GAAGCAGACAAGGATAAAGA

Downstream 100 bases:

>100_bases
TGGAAATAGAGTCGAAATTCGATATCGATGACAGAAAACGGCTTGTATATTCCACTATTCGCCCCAATATGCCTTTTCGA
ATCAGCCAGCTACCAAGAGG

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MNAAKRQEIFRRLRDDNPHPTTELEYNTPFELLISVLLSAQATDVGVNKATRRLYPVANTPAAMLALGEEGLAEYIKTIG
LYKTKARNVIATCRLLLEKHGGEVPQTREALEALPGVGRKTANVVLNTAFGQPTIAVDTHIFRVSNRTRIAPGKDVREVE
DKLERFVPAEFKLDAHHWLILLGRYVCKARKPECHRCAIADLCEYPAKPL

Sequences:

>Translated_210_residues
MNAAKRQEIFRRLRDDNPHPTTELEYNTPFELLISVLLSAQATDVGVNKATRRLYPVANTPAAMLALGEEGLAEYIKTIG
LYKTKARNVIATCRLLLEKHGGEVPQTREALEALPGVGRKTANVVLNTAFGQPTIAVDTHIFRVSNRTRIAPGKDVREVE
DKLERFVPAEFKLDAHHWLILLGRYVCKARKPECHRCAIADLCEYPAKPL
>Mature_210_residues
MNAAKRQEIFRRLRDDNPHPTTELEYNTPFELLISVLLSAQATDVGVNKATRRLYPVANTPAAMLALGEEGLAEYIKTIG
LYKTKARNVIATCRLLLEKHGGEVPQTREALEALPGVGRKTANVVLNTAFGQPTIAVDTHIFRVSNRTRIAPGKDVREVE
DKLERFVPAEFKLDAHHWLILLGRYVCKARKPECHRCAIADLCEYPAKPL

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=31.638418079096, Blast_Score=98, Evalue=6e-21,
Organism=Homo sapiens, GI190358497, Length=129, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI115298648, Length=129, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI6912520, Length=129, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI115298654, Length=129, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI115298652, Length=129, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI115298650, Length=129, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=74.5192307692308, Blast_Score=324, Evalue=3e-90,
Organism=Escherichia coli, GI1789331, Length=129, Percent_Identity=34.1085271317829, Blast_Score=69, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=31.0734463276836, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319304, Length=174, Percent_Identity=26.4367816091954, Blast_Score=70, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324530, Length=190, Percent_Identity=25.7894736842105, Blast_Score=68, Evalue=8e-13,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=30.5084745762712, Blast_Score=98, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23509; Mature: 23509

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAAKRQEIFRRLRDDNPHPTTELEYNTPFELLISVLLSAQATDVGVNKATRRLYPVANT
CCHHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCC
PAAMLALGEEGLAEYIKTIGLYKTKARNVIATCRLLLEKHGGEVPQTREALEALPGVGRK
CHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
TANVVLNTAFGQPTIAVDTHIFRVSNRTRIAPGKDVREVEDKLERFVPAEFKLDAHHWLI
HHHHEEECCCCCCEEEECCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEECHHHHHH
LLGRYVCKARKPECHRCAIADLCEYPAKPL
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNAAKRQEIFRRLRDDNPHPTTELEYNTPFELLISVLLSAQATDVGVNKATRRLYPVANT
CCHHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCC
PAAMLALGEEGLAEYIKTIGLYKTKARNVIATCRLLLEKHGGEVPQTREALEALPGVGRK
CHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
TANVVLNTAFGQPTIAVDTHIFRVSNRTRIAPGKDVREVEDKLERFVPAEFKLDAHHWLI
HHHHEEECCCCCCEEEECCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEECHHHHHH
LLGRYVCKARKPECHRCAIADLCEYPAKPL
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]