Click here to switch to the map view.

The map label for this gene is fruA [H]

Identifier: 34498509

GI number: 34498509

Start: 3332748

End: 3334412

Strand: Direct

Name: fruA [H]

Synonym: CV_3054

Alternate gene names: 34498509

Gene position: 3332748-3334412 (Clockwise)

Preceding gene: 34498508

Following gene: 34498510

Centisome position: 70.15

GC content: 71.71

Gene sequence:

>1665_bases
ATGAGCATCGTCGCCATCATCCGCGCCCCGGAGCGCAGCACCCAGGCCAGGCTGGCCGCCGAAGCGCTGCGCCAGGCCGC
CCAGCGGCAGGGCAAACGGATAGACATCGAGATAGACGGCCAGCCCGCGCTGGGCGCTGACATCCTGCAGGCCGCCAGCC
AGATCCTGCTGGTGGACTGTCCCGACGACGCCCGCGCCGGGCAAAAACCCGCGCGCCGCGCCACGCTGGACGCGGTATTG
GCCGATCCGGCCGCGCAGCTGGACGGCGCCGCGGCTGCCGAGCGCGCGCTCAGCATCGTCGCCATCACCGGCTGCCCCAC
CGGCATCGCCCACACCTTCATGGCGGCCGAGGGGCTGGTCCAGGGCGCCAAGGCGCTGGGCCACGCGATGCGGGTGGAAA
CCCAGGGCTCGGTCGGCGCGCAGAACGCGTTGACCGACGCCGAGATCGCCGCCGCCGACTTGGTGGTGATCGCCGCCGAC
ACCCAGGTGCAGCTGGACCGCTTCGCCGGCAAACGGCTGTTCAAGAGCGGCACCAAGGCCGCCATCGGCGACGGCAAGGC
GCTGATCCGCCGCGCGATAGCGGAGGCGCAGCCCTGGAGCGGCGCGGCCGCGCCCGCCGCCCAGGCCGCCGGCAAGGAGA
AAACCAAGGACGGCCCCTATCGCCACCTGATGACCGGCGTGTCCTTCATGCTGCCCTTCGTCACCGCCGGCGGCATCCTG
ATCGCGCTCGCCTTCGCGCTGGGCGGCATCTACGCCTTCGACGACGCGCACAAGGGCACGCTGGCCTGGTCGCTGTTCCA
GATCGGCGCCAAATCGGCATTCGCGCTGATGGTGCCGATACTGGCCGGCTACATCGCCTACTCCGTCGCCGACCGCCCCG
GCATCGCCCCCGGCATGGTGGGCGGCATGCTGGCCGCCAGCCTGGGATCGGGCTTCCTCGGCGGCATCGTCGCCGGCTTC
ATCGCAGGCTACGGCACGCGGGAGCTGAACCGCCGCATCCAGCTGGGCCAGCATCTGGACGGCCTGAAGCCGGTGCTGAT
CCTGCCGGTGCTGGGCTCGCTGCTGACCGGCCTCTTGATGATCTACGTGGTGGGCCAGCCGGTGGCCGCCGTGCTGGCGG
CGCTGACCGACGCGCTGAAGGGCATGCAGGGCAGCAGCGCGCTGGCATTGGGCGCGCTGCTGGGCGCGATGATGGCCTTC
GACATGGGCGGCCCGGTCAACAAGGCGGCCTACGCCTTCGCCACCGGCCTGATCGCCAGCCAGGTCTACACGCCGATGGC
GGCGGTGATGGCCGCCGGCATGACGCCGCCGCTGGGCATCGCGCTGGCCACCCGCCTGTTCCGAGACCGCTTCACCGTCG
ACGAGCATGAGGCCGGCAAGGCTGCCGCCGTGCTGGGCCTGGCCTTCATCAGCGAAGGCGCCATCCCCTTCGCCGCGCGC
GATCCTTTCCGCGTGATTCCGGCGCTGATGGCCGGCTCGGCGCTGGCCGGCGCCGTCTCGATGACCATGGGCGTGGAACT
GAAAGTGCCGCACGGCGGCGTGTTCGTGCTGCCCATCCCCAACGCGGTCGAACACCTCGCCAGCTACCTGGCGGCGCTCG
CCGCCGGCACCGCCGTCACCGCGCTGGCGCTGGGCCTGCTGAAGAAGAGAATCCCCCAGGCCTGA

Upstream 100 bases:

>100_bases
CCTGCCGCCGGAACAACAGATACGAGAGCAGGCCGCCGCCGTCAGCCTGCAAACCCTGGCCGCGGCCTGAGCCGCGTCCG
CCCAGAAACAGGGGAAACAC

Downstream 100 bases:

>100_bases
TCTCGCCCGCTTGCGCGCGGCATCCTCCCAGGCCCGGCTCAGCCGGGCCTGTCCCTTTCTTTCCCGCGGCCTCGCCAGCT
TATTGCGGTCCAGCCATCCC

Product: protein-N p-phosphohistidine-sugar phosphotransferase

Products: NA

Alternate protein names: EIIBC-Fru; EII-Fru; Fructose-specific phosphotransferase enzyme IIB component; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MSIVAIIRAPERSTQARLAAEALRQAAQRQGKRIDIEIDGQPALGADILQAASQILLVDCPDDARAGQKPARRATLDAVL
ADPAAQLDGAAAAERALSIVAITGCPTGIAHTFMAAEGLVQGAKALGHAMRVETQGSVGAQNALTDAEIAAADLVVIAAD
TQVQLDRFAGKRLFKSGTKAAIGDGKALIRRAIAEAQPWSGAAAPAAQAAGKEKTKDGPYRHLMTGVSFMLPFVTAGGIL
IALAFALGGIYAFDDAHKGTLAWSLFQIGAKSAFALMVPILAGYIAYSVADRPGIAPGMVGGMLAASLGSGFLGGIVAGF
IAGYGTRELNRRIQLGQHLDGLKPVLILPVLGSLLTGLLMIYVVGQPVAAVLAALTDALKGMQGSSALALGALLGAMMAF
DMGGPVNKAAYAFATGLIASQVYTPMAAVMAAGMTPPLGIALATRLFRDRFTVDEHEAGKAAAVLGLAFISEGAIPFAAR
DPFRVIPALMAGSALAGAVSMTMGVELKVPHGGVFVLPIPNAVEHLASYLAALAAGTAVTALALGLLKKRIPQA

Sequences:

>Translated_554_residues
MSIVAIIRAPERSTQARLAAEALRQAAQRQGKRIDIEIDGQPALGADILQAASQILLVDCPDDARAGQKPARRATLDAVL
ADPAAQLDGAAAAERALSIVAITGCPTGIAHTFMAAEGLVQGAKALGHAMRVETQGSVGAQNALTDAEIAAADLVVIAAD
TQVQLDRFAGKRLFKSGTKAAIGDGKALIRRAIAEAQPWSGAAAPAAQAAGKEKTKDGPYRHLMTGVSFMLPFVTAGGIL
IALAFALGGIYAFDDAHKGTLAWSLFQIGAKSAFALMVPILAGYIAYSVADRPGIAPGMVGGMLAASLGSGFLGGIVAGF
IAGYGTRELNRRIQLGQHLDGLKPVLILPVLGSLLTGLLMIYVVGQPVAAVLAALTDALKGMQGSSALALGALLGAMMAF
DMGGPVNKAAYAFATGLIASQVYTPMAAVMAAGMTPPLGIALATRLFRDRFTVDEHEAGKAAAVLGLAFISEGAIPFAAR
DPFRVIPALMAGSALAGAVSMTMGVELKVPHGGVFVLPIPNAVEHLASYLAALAAGTAVTALALGLLKKRIPQA
>Mature_553_residues
SIVAIIRAPERSTQARLAAEALRQAAQRQGKRIDIEIDGQPALGADILQAASQILLVDCPDDARAGQKPARRATLDAVLA
DPAAQLDGAAAAERALSIVAITGCPTGIAHTFMAAEGLVQGAKALGHAMRVETQGSVGAQNALTDAEIAAADLVVIAADT
QVQLDRFAGKRLFKSGTKAAIGDGKALIRRAIAEAQPWSGAAAPAAQAAGKEKTKDGPYRHLMTGVSFMLPFVTAGGILI
ALAFALGGIYAFDDAHKGTLAWSLFQIGAKSAFALMVPILAGYIAYSVADRPGIAPGMVGGMLAASLGSGFLGGIVAGFI
AGYGTRELNRRIQLGQHLDGLKPVLILPVLGSLLTGLLMIYVVGQPVAAVLAALTDALKGMQGSSALALGALLGAMMAFD
MGGPVNKAAYAFATGLIASQVYTPMAAVMAAGMTPPLGIALATRLFRDRFTVDEHEAGKAAAVLGLAFISEGAIPFAARD
PFRVIPALMAGSALAGAVSMTMGVELKVPHGGVFVLPIPNAVEHLASYLAALAAGTAVTALALGLLKKRIPQA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=453, Percent_Identity=56.0706401766004, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI1786951, Length=462, Percent_Identity=38.3116883116883, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI87082348, Length=469, Percent_Identity=36.0341151385927, Blast_Score=248, Evalue=1e-66,
Organism=Escherichia coli, GI1790386, Length=300, Percent_Identity=44.6666666666667, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1788729, Length=410, Percent_Identity=28.0487804878049, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1790387, Length=94, Percent_Identity=42.5531914893617, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1788730, Length=95, Percent_Identity=34.7368421052632, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 56383; Mature: 56252

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIVAIIRAPERSTQARLAAEALRQAAQRQGKRIDIEIDGQPALGADILQAASQILLVDC
CCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHCCCEEEEEC
PDDARAGQKPARRATLDAVLADPAAQLDGAAAAERALSIVAITGCPTGIAHTFMAAEGLV
CCHHCCCCCCHHHHHHHHHHCCCHHHHCCHHHHHHCEEEEEEECCCHHHHHHHHHHHHHH
QGAKALGHAMRVETQGSVGAQNALTDAEIAAADLVVIAADTQVQLDRFAGKRLFKSGTKA
HHHHHHHHHHEEECCCCCCCHHCCCHHHHHHHCEEEEECCCHHHHHHHHHHHHHHCCCCC
AIGDGKALIRRAIAEAQPWSGAAAPAAQAAGKEKTKDGPYRHLMTGVSFMLPFVTAGGIL
CCCCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IALAFALGGIYAFDDAHKGTLAWSLFQIGAKSAFALMVPILAGYIAYSVADRPGIAPGMV
HHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
GGMLAASLGSGFLGGIVAGFIAGYGTRELNRRIQLGQHLDGLKPVLILPVLGSLLTGLLM
HHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
IYVVGQPVAAVLAALTDALKGMQGSSALALGALLGAMMAFDMGGPVNKAAYAFATGLIAS
HHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
QVYTPMAAVMAAGMTPPLGIALATRLFRDRFTVDEHEAGKAAAVLGLAFISEGAIPFAAR
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
DPFRVIPALMAGSALAGAVSMTMGVELKVPHGGVFVLPIPNAVEHLASYLAALAAGTAVT
CHHHHHHHHHHCHHHHHHHHHHCCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
ALALGLLKKRIPQA
HHHHHHHHHHCCCC
>Mature Secondary Structure 
SIVAIIRAPERSTQARLAAEALRQAAQRQGKRIDIEIDGQPALGADILQAASQILLVDC
CEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHCCCEEEEEC
PDDARAGQKPARRATLDAVLADPAAQLDGAAAAERALSIVAITGCPTGIAHTFMAAEGLV
CCHHCCCCCCHHHHHHHHHHCCCHHHHCCHHHHHHCEEEEEEECCCHHHHHHHHHHHHHH
QGAKALGHAMRVETQGSVGAQNALTDAEIAAADLVVIAADTQVQLDRFAGKRLFKSGTKA
HHHHHHHHHHEEECCCCCCCHHCCCHHHHHHHCEEEEECCCHHHHHHHHHHHHHHCCCCC
AIGDGKALIRRAIAEAQPWSGAAAPAAQAAGKEKTKDGPYRHLMTGVSFMLPFVTAGGIL
CCCCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IALAFALGGIYAFDDAHKGTLAWSLFQIGAKSAFALMVPILAGYIAYSVADRPGIAPGMV
HHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
GGMLAASLGSGFLGGIVAGFIAGYGTRELNRRIQLGQHLDGLKPVLILPVLGSLLTGLLM
HHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
IYVVGQPVAAVLAALTDALKGMQGSSALALGALLGAMMAFDMGGPVNKAAYAFATGLIAS
HHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
QVYTPMAAVMAAGMTPPLGIALATRLFRDRFTVDEHEAGKAAAVLGLAFISEGAIPFAAR
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
DPFRVIPALMAGSALAGAVSMTMGVELKVPHGGVFVLPIPNAVEHLASYLAALAAGTAVT
CHHHHHHHHHHCHHHHHHHHHHCCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
ALALGLLKKRIPQA
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1655739; 12024217; 1650911 [H]