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The map label for this gene is fruB [H]

Identifier: 34498507

GI number: 34498507

Start: 3329263

End: 3331764

Strand: Direct

Name: fruB [H]

Synonym: CV_3052

Alternate gene names: 34498507

Gene position: 3329263-3331764 (Clockwise)

Preceding gene: 34498505

Following gene: 34498508

Centisome position: 70.07

GC content: 72.1

Gene sequence:

>2502_bases
GTGTCCGACGCCCTGCTCACTCCGCAACTGATACGCCTGGGCTGCCAGCCCGCCGGCAAAGAGGCCGCCATCCGCGAGGC
CGGCCGCATGCTGGCCGACGCCGGCTGCATCGCGCCCGATTACATAGACAGCCTGCTGCGCCGGGAGGCGGTAGCCAACA
CCTACCTGGGCAACGGCATCGCCATTCCCCACGGCATGGTGGAAGACCGCGCGATGGTGCTGCGCACCGGCGTCGCCATC
CTGCAGATTCCAGCCGGACTGGAATGGAACCCCGGCCAGCGCACCCACCTGCTGTGCGCGATCGCCGCGCGTTCCGACGA
CCACCTGGTGCTGCTGCGCCAGCTGACGCGGCTGCTGCAAGACGAGGCCAGGCTGCTGCCGCTGTTTTCCACCCGGGACA
GCGCCGACCTGATCGCCGCGCTGGAACAACCGGCGGAAACCCCGCAAGCGGACGTCGAGGCGCAAGACCTGGACGTGCGG
GACGAATGGCGGCTCGACTACCCCAACGGCTTGCACGCCCGCCCCGCCGCGCTGTGGGTGGAGGCAGCGCGGCGCAGTCC
GGCCCAATTGCAGGTGCGCCACGGCGGCCGCGCCGCCGACGCCAAGAACCTGATCTCGCTGCTGCAGCTGGGCCTGCGCC
AGGGCGACCTGGTCGCCGTATCCGCCCGCGGCCCGCAGGCCGAGGAAGGCGTGCGCCAGTTGCTGAAGGCGATGCGCGAA
GCCAGCCTGGCGGAGCAGACCGCGGCGGCCATCGCCGCTCAGCGCGCCGCCGGCGCGCGCAAAACCGCCGGCTGGCAGCC
GGCCGGATCTTTGATTTCCCTGCCCGGCATCGCCGCCAGTCCGGGCCTCGCCATCGGCACCGTGCGCGTCCTGGCCGGAG
AGCGGCTGCAAGTGCCCGACCGGCCCGTCAGCCTGGCCGATGGCGGCGACCGCCTGCACGAAGCGCTGGCCGCCACCCGC
CAACAGCTGGCCGAACTGGCCGATTCCACCCAGGCGACCCTGGGCGCCGGCGAAGCCGGCATCTTCCGTGCCCAGGCGGA
ACTGCTGAACGACACCGACCTGATCACGCTAAGCTGCCAGCTGATGGTGGCCGGCCACGGCGTGGAGTGGTCATGGCATC
AGGCGGTGGAGCGCCTGGCGGAAAAAATCTCCGCCTTGGGCAACCCGCTGCTGGCGGCGCGCGCCGCCGACCTGCGCGAC
GTCGGCCGTCGGGTGCTGTTCCACCTGGACCCGGCGCTGCAGGGCAGCGCGCCGGCCGAATTCGGCCCCGACACCCTGCT
GCTGGCCGCCGATCTCTCGCCGTCCGACACCGCCAGCCTGGACCTTTCCCGCGTCAAGGGGTTGGCCACCGCTCAAGGCG
GCCCCACCGCCCACACCGCCATCCTGGCCCGGACGCTGGGCCTGCCGGCGCTGGTGGCCGCCGGCGCGGCGTTGCTGGAC
GTGGCCGACGGCAGCCCCGCCATCCTGGACGGCGACGCCGGCCGGCTGTACCTGAATCCGGGCGACGCCGACCTCGCCTC
CGCCCGCGCCTGGCAGCAGCGCCAGCTGGAAGACCAGCAAAAACAACAGGCGGAACGGCAGCAGCCCGGACAGACCGCCG
ACGGCAAACGCATCGAAATCGCCGCCAACATCAACCGCCCCGACCAGGCCGCCGCCGCGCTGGACGCCGGCGCCGAGGGC
GTGGGCCTGATGCGCACCGAGTTCCTGTTCCTGGAACGCGAGGCCGCGCCGGACGAAGAAGAGCAGCTGCGCACCTATCT
GGCCATGCAGCAGGCGCTGCAAGGCCGGCCGCTGATCGTCCGCGCGCTGGACATCGGCGGCGACAAGCAGGTGCCCTATC
TGAACCTGCCGCATGAGGACAATCCCTTCCTCGGCATGCGCGGCGCGCGGCTGCTGCTGTCCCGCCGCGAGCTGCTGGAG
CCGCAGCTGCGCGCGCTGTACCGCGCCGCCAAGGCCGGCAAGCCGCTATCCATCATGTTCCCGATGATCAGCTCGCTGAA
GGAGATCACCCGCCTCAAGGGCCTGTGCGAGGGCGTGCGCAAGGAACTGGACGCGCCGCAGGTGCCGATCGGCATCATGG
TGGAGGTGCCGGCCGCCGCGCTGCTGGCCGACCAGTTCGCCCCCTATGTCGACTTCTTCTCCATCGGCACCAACGACCTG
ACCCAGTACGCGCTGGCGATGGACCGCCAGCACCCCGAGCTGGCCGCCGAGGCCGACAGCCTCCACCCCGCCGTGCTGCG
GCTGATCCGCCAGACCGTGCAAGGCGCGGCGGCGCACCGCCGTTGGGTGGGCGTGTGCGGCGGCATCGCCGGCGATCCGC
AGGGCGCGGCGGTGCTGGCCGGCCTGGGCGTGGACGAGCTGTCGATGAGCCCGCACGACATCGCCGCGGTGAAGGCGATG
CTGCGCCGCCACAGCCACGCCAGGCTGCGCGAGCTGGCGGAGCAGGCGCTCGCCTGCGACAGCGCCGAGGAAGTGCGGCA
ATTGCTGGAGGCGCTGGCATGA

Upstream 100 bases:

>100_bases
TTGCGCGACAAGTTTGATTTTAACCAGTTCTGTCCGATTGACAGCAAAAATGTAATCGTTTTCAATGCCAGCCATATCGA
GTCCAAGAACGAGGTACGCC

Downstream 100 bases:

>100_bases
GCGGCGCGATACACACCGTCACCCCCAATCCGGCGCTCGACCAGACCGTGACCCTGGACGCCTTGCGGACCGGCGCCGTC
AACCTGGCGCGCGCCGCCCA

Product: phosphotransferase system

Products: NA

Alternate protein names: MTP; Phosphoenolpyruvate-protein phosphotransferase; Phosphotransferase system enzyme I; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; EIII-Fru; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 833; Mature: 832

Protein sequence:

>833_residues
MSDALLTPQLIRLGCQPAGKEAAIREAGRMLADAGCIAPDYIDSLLRREAVANTYLGNGIAIPHGMVEDRAMVLRTGVAI
LQIPAGLEWNPGQRTHLLCAIAARSDDHLVLLRQLTRLLQDEARLLPLFSTRDSADLIAALEQPAETPQADVEAQDLDVR
DEWRLDYPNGLHARPAALWVEAARRSPAQLQVRHGGRAADAKNLISLLQLGLRQGDLVAVSARGPQAEEGVRQLLKAMRE
ASLAEQTAAAIAAQRAAGARKTAGWQPAGSLISLPGIAASPGLAIGTVRVLAGERLQVPDRPVSLADGGDRLHEALAATR
QQLAELADSTQATLGAGEAGIFRAQAELLNDTDLITLSCQLMVAGHGVEWSWHQAVERLAEKISALGNPLLAARAADLRD
VGRRVLFHLDPALQGSAPAEFGPDTLLLAADLSPSDTASLDLSRVKGLATAQGGPTAHTAILARTLGLPALVAAGAALLD
VADGSPAILDGDAGRLYLNPGDADLASARAWQQRQLEDQQKQQAERQQPGQTADGKRIEIAANINRPDQAAAALDAGAEG
VGLMRTEFLFLEREAAPDEEEQLRTYLAMQQALQGRPLIVRALDIGGDKQVPYLNLPHEDNPFLGMRGARLLLSRRELLE
PQLRALYRAAKAGKPLSIMFPMISSLKEITRLKGLCEGVRKELDAPQVPIGIMVEVPAAALLADQFAPYVDFFSIGTNDL
TQYALAMDRQHPELAAEADSLHPAVLRLIRQTVQGAAAHRRWVGVCGGIAGDPQGAAVLAGLGVDELSMSPHDIAAVKAM
LRRHSHARLRELAEQALACDSAEEVRQLLEALA

Sequences:

>Translated_833_residues
MSDALLTPQLIRLGCQPAGKEAAIREAGRMLADAGCIAPDYIDSLLRREAVANTYLGNGIAIPHGMVEDRAMVLRTGVAI
LQIPAGLEWNPGQRTHLLCAIAARSDDHLVLLRQLTRLLQDEARLLPLFSTRDSADLIAALEQPAETPQADVEAQDLDVR
DEWRLDYPNGLHARPAALWVEAARRSPAQLQVRHGGRAADAKNLISLLQLGLRQGDLVAVSARGPQAEEGVRQLLKAMRE
ASLAEQTAAAIAAQRAAGARKTAGWQPAGSLISLPGIAASPGLAIGTVRVLAGERLQVPDRPVSLADGGDRLHEALAATR
QQLAELADSTQATLGAGEAGIFRAQAELLNDTDLITLSCQLMVAGHGVEWSWHQAVERLAEKISALGNPLLAARAADLRD
VGRRVLFHLDPALQGSAPAEFGPDTLLLAADLSPSDTASLDLSRVKGLATAQGGPTAHTAILARTLGLPALVAAGAALLD
VADGSPAILDGDAGRLYLNPGDADLASARAWQQRQLEDQQKQQAERQQPGQTADGKRIEIAANINRPDQAAAALDAGAEG
VGLMRTEFLFLEREAAPDEEEQLRTYLAMQQALQGRPLIVRALDIGGDKQVPYLNLPHEDNPFLGMRGARLLLSRRELLE
PQLRALYRAAKAGKPLSIMFPMISSLKEITRLKGLCEGVRKELDAPQVPIGIMVEVPAAALLADQFAPYVDFFSIGTNDL
TQYALAMDRQHPELAAEADSLHPAVLRLIRQTVQGAAAHRRWVGVCGGIAGDPQGAAVLAGLGVDELSMSPHDIAAVKAM
LRRHSHARLRELAEQALACDSAEEVRQLLEALA
>Mature_832_residues
SDALLTPQLIRLGCQPAGKEAAIREAGRMLADAGCIAPDYIDSLLRREAVANTYLGNGIAIPHGMVEDRAMVLRTGVAIL
QIPAGLEWNPGQRTHLLCAIAARSDDHLVLLRQLTRLLQDEARLLPLFSTRDSADLIAALEQPAETPQADVEAQDLDVRD
EWRLDYPNGLHARPAALWVEAARRSPAQLQVRHGGRAADAKNLISLLQLGLRQGDLVAVSARGPQAEEGVRQLLKAMREA
SLAEQTAAAIAAQRAAGARKTAGWQPAGSLISLPGIAASPGLAIGTVRVLAGERLQVPDRPVSLADGGDRLHEALAATRQ
QLAELADSTQATLGAGEAGIFRAQAELLNDTDLITLSCQLMVAGHGVEWSWHQAVERLAEKISALGNPLLAARAADLRDV
GRRVLFHLDPALQGSAPAEFGPDTLLLAADLSPSDTASLDLSRVKGLATAQGGPTAHTAILARTLGLPALVAAGAALLDV
ADGSPAILDGDAGRLYLNPGDADLASARAWQQRQLEDQQKQQAERQQPGQTADGKRIEIAANINRPDQAAAALDAGAEGV
GLMRTEFLFLEREAAPDEEEQLRTYLAMQQALQGRPLIVRALDIGGDKQVPYLNLPHEDNPFLGMRGARLLLSRRELLEP
QLRALYRAAKAGKPLSIMFPMISSLKEITRLKGLCEGVRKELDAPQVPIGIMVEVPAAALLADQFAPYVDFFSIGTNDLT
QYALAMDRQHPELAAEADSLHPAVLRLIRQTVQGAAAHRRWVGVCGGIAGDPQGAAVLAGLGVDELSMSPHDIAAVKAML
RRHSHARLRELAEQALACDSAEEVRQLLEALA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=570, Percent_Identity=38.7719298245614, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1788726, Length=678, Percent_Identity=35.3982300884956, Blast_Score=340, Evalue=2e-94,
Organism=Escherichia coli, GI48994992, Length=698, Percent_Identity=33.3810888252149, Blast_Score=307, Evalue=1e-84,
Organism=Escherichia coli, GI1789193, Length=500, Percent_Identity=36.2, Blast_Score=291, Evalue=1e-79,
Organism=Escherichia coli, GI1787994, Length=421, Percent_Identity=28.2660332541568, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI1788494, Length=156, Percent_Identity=42.3076923076923, Blast_Score=116, Evalue=6e-27,
Organism=Escherichia coli, GI226510935, Length=354, Percent_Identity=27.4011299435028, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1790027, Length=117, Percent_Identity=41.025641025641, Blast_Score=92, Evalue=2e-19,
Organism=Escherichia coli, GI1788755, Length=81, Percent_Identity=40.7407407407407, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 88815; Mature: 88683

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00369 PTS_HPR_HIS ; PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDALLTPQLIRLGCQPAGKEAAIREAGRMLADAGCIAPDYIDSLLRREAVANTYLGNGI
CCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCE
AIPHGMVEDRAMVLRTGVAILQIPAGLEWNPGQRTHLLCAIAARSDDHLVLLRQLTRLLQ
ECCCCCHHHHHHHHHHCEEEEEECCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHH
DEARLLPLFSTRDSADLIAALEQPAETPQADVEAQDLDVRDEWRLDYPNGLHARPAALWV
HHHHEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
EAARRSPAQLQVRHGGRAADAKNLISLLQLGLRQGDLVAVSARGPQAEEGVRQLLKAMRE
HHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHH
ASLAEQTAAAIAAQRAAGARKTAGWQPAGSLISLPGIAASPGLAIGTVRVLAGERLQVPD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHCCCCCCCCC
RPVSLADGGDRLHEALAATRQQLAELADSTQATLGAGEAGIFRAQAELLNDTDLITLSCQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEEE
LMVAGHGVEWSWHQAVERLAEKISALGNPLLAARAADLRDVGRRVLFHLDPALQGSAPAE
EEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
FGPDTLLLAADLSPSDTASLDLSRVKGLATAQGGPTAHTAILARTLGLPALVAAGAALLD
CCCCEEEEEECCCCCCCCCHHHHHHCCHHCCCCCCHHHHHHHHHHHCCHHHHHCCHHEEE
VADGSPAILDGDAGRLYLNPGDADLASARAWQQRQLEDQQKQQAERQQPGQTADGKRIEI
ECCCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
AANINRPDQAAAALDAGAEGVGLMRTEFLFLEREAAPDEEEQLRTYLAMQQALQGRPLIV
EECCCCCHHHHHHHHCCCCCCCHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHCCCCEEE
RALDIGGDKQVPYLNLPHEDNPFLGMRGARLLLSRRELLEPQLRALYRAAKAGKPLSIMF
EEEECCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHH
PMISSLKEITRLKGLCEGVRKELDAPQVPIGIMVEVPAAALLADQFAPYVDFFSIGTNDL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHCHHHHHHHCCCHHH
TQYALAMDRQHPELAAEADSLHPAVLRLIRQTVQGAAAHRRWVGVCGGIAGDPQGAAVLA
HHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
GLGVDELSMSPHDIAAVKAMLRRHSHARLRELAEQALACDSAEEVRQLLEALA
ECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
>Mature Secondary Structure 
SDALLTPQLIRLGCQPAGKEAAIREAGRMLADAGCIAPDYIDSLLRREAVANTYLGNGI
CCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCE
AIPHGMVEDRAMVLRTGVAILQIPAGLEWNPGQRTHLLCAIAARSDDHLVLLRQLTRLLQ
ECCCCCHHHHHHHHHHCEEEEEECCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHH
DEARLLPLFSTRDSADLIAALEQPAETPQADVEAQDLDVRDEWRLDYPNGLHARPAALWV
HHHHEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
EAARRSPAQLQVRHGGRAADAKNLISLLQLGLRQGDLVAVSARGPQAEEGVRQLLKAMRE
HHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHH
ASLAEQTAAAIAAQRAAGARKTAGWQPAGSLISLPGIAASPGLAIGTVRVLAGERLQVPD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHCCCCCCCCC
RPVSLADGGDRLHEALAATRQQLAELADSTQATLGAGEAGIFRAQAELLNDTDLITLSCQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEEE
LMVAGHGVEWSWHQAVERLAEKISALGNPLLAARAADLRDVGRRVLFHLDPALQGSAPAE
EEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
FGPDTLLLAADLSPSDTASLDLSRVKGLATAQGGPTAHTAILARTLGLPALVAAGAALLD
CCCCEEEEEECCCCCCCCCHHHHHHCCHHCCCCCCHHHHHHHHHHHCCHHHHHCCHHEEE
VADGSPAILDGDAGRLYLNPGDADLASARAWQQRQLEDQQKQQAERQQPGQTADGKRIEI
ECCCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
AANINRPDQAAAALDAGAEGVGLMRTEFLFLEREAAPDEEEQLRTYLAMQQALQGRPLIV
EECCCCCHHHHHHHHCCCCCCCHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHCCCCEEE
RALDIGGDKQVPYLNLPHEDNPFLGMRGARLLLSRRELLEPQLRALYRAAKAGKPLSIMF
EEEECCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHH
PMISSLKEITRLKGLCEGVRKELDAPQVPIGIMVEVPAAALLADQFAPYVDFFSIGTNDL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHCHHHHHHHCCCHHH
TQYALAMDRQHPELAAEADSLHPAVLRLIRQTVQGAAAHRRWVGVCGGIAGDPQGAAVLA
HHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
GLGVDELSMSPHDIAAVKAMLRRHSHARLRELAEQALACDSAEEVRQLLEALA
ECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7496537; 12024217; 1655739 [H]