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The map label for this gene is amiD [H]

Identifier: 34498486

GI number: 34498486

Start: 3310655

End: 3311428

Strand: Reverse

Name: amiD [H]

Synonym: CV_3031

Alternate gene names: 34498486

Gene position: 3311428-3310655 (Counterclockwise)

Preceding gene: 34498487

Following gene: 34498485

Centisome position: 69.7

GC content: 44.7

Gene sequence:

>774_bases
ATGTATCAAATAGATTATAATAGCTATCGTGCTATTAAGGGGTTTAATCGCCGTGTACGTTTTTTGGTTATGCATTATAC
GGCAGCGGATTTTAAAAGATCGATTGAGGATCTTGCGGTAAATGGAAAGGTTAGCGTTCACTATCTGATTCCGGATCCTA
CGGAGAAAACTTATATAGATGCCGGTTTCAAGGAGTTGCAGATTTTTAATCTGGTGGATGAAAGCGAGCGTGCATGGCAT
GCTGGGGTTAGTTACTGGGCTGGGCGTGTCAATATCAATGACTCTTCAATCGGGATCGAAAATGTAAATCTCGCAGCCGA
TCATGGTGATGGAATCATATTCCTTCCCTATAATGAGGCGCAGGTTAAAGCCATCAAATCGCTTGCGCTGAATATTCTCC
AGCGTTATCCGGATATTTCTCCAACTAATGTGGTTGGGCATAGCGACGTCGCTCCTGGCCGAAAAAGCGACCCGGGCCCG
TTATTCCCATGGCAGGAGCTTTACAAGGAGGGTATAGGTGCATGGTATGACGATGCGACCAAGAAAGAGTATGAGAAGAT
CTTTTTCGAGCATGGGCTGCCCATTGAAAAGGAAATCATCGAAAAATTAGGCATTTATGGTTATGACGTTTCGCATGCGA
GTCATCCCGATGGCCTGAAAGCGCTTGTGCGATCTTTTCAAATGCATTTTCGCCCGGCTGATTACGATGGGAGAGTGGAT
GTTGAAACCACGGCGATCTTGTATGCGCTGGTGAAGAAATATTTCAATAAATAA

Upstream 100 bases:

>100_bases
TTTTGTTGATTAAATATTTTGCGATATGTTGAGATTCAATAAAAGAAGCCATTTATATCGCGACGTACATTTCATTTTTT
AAATTATTTGAGGTGGTGGT

Downstream 100 bases:

>100_bases
TTTCCGCTGCGGGAATCTGCAAGGCTTGCGAGCCTTGCGGCCCGGCAGACAGCGAGAGGTTTTTCATCTTGATGGTCCGA
TGGCTATTTCCAGTGCGCAA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MYQIDYNSYRAIKGFNRRVRFLVMHYTAADFKRSIEDLAVNGKVSVHYLIPDPTEKTYIDAGFKELQIFNLVDESERAWH
AGVSYWAGRVNINDSSIGIENVNLAADHGDGIIFLPYNEAQVKAIKSLALNILQRYPDISPTNVVGHSDVAPGRKSDPGP
LFPWQELYKEGIGAWYDDATKKEYEKIFFEHGLPIEKEIIEKLGIYGYDVSHASHPDGLKALVRSFQMHFRPADYDGRVD
VETTAILYALVKKYFNK

Sequences:

>Translated_257_residues
MYQIDYNSYRAIKGFNRRVRFLVMHYTAADFKRSIEDLAVNGKVSVHYLIPDPTEKTYIDAGFKELQIFNLVDESERAWH
AGVSYWAGRVNINDSSIGIENVNLAADHGDGIIFLPYNEAQVKAIKSLALNILQRYPDISPTNVVGHSDVAPGRKSDPGP
LFPWQELYKEGIGAWYDDATKKEYEKIFFEHGLPIEKEIIEKLGIYGYDVSHASHPDGLKALVRSFQMHFRPADYDGRVD
VETTAILYALVKKYFNK
>Mature_257_residues
MYQIDYNSYRAIKGFNRRVRFLVMHYTAADFKRSIEDLAVNGKVSVHYLIPDPTEKTYIDAGFKELQIFNLVDESERAWH
AGVSYWAGRVNINDSSIGIENVNLAADHGDGIIFLPYNEAQVKAIKSLALNILQRYPDISPTNVVGHSDVAPGRKSDPGP
LFPWQELYKEGIGAWYDDATKKEYEKIFFEHGLPIEKEIIEKLGIYGYDVSHASHPDGLKALVRSFQMHFRPADYDGRVD
VETTAILYALVKKYFNK

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=254, Percent_Identity=42.5196850393701, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1786300, Length=122, Percent_Identity=38.5245901639344, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 29312; Mature: 29312

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQIDYNSYRAIKGFNRRVRFLVMHYTAADFKRSIEDLAVNGKVSVHYLIPDPTEKTYID
CEEECCCCHHHHHHHCCEEEEEEEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEC
AGFKELQIFNLVDESERAWHAGVSYWAGRVNINDSSIGIENVNLAADHGDGIIFLPYNEA
CCHHHEEEEEHHCCCCHHHHHHHHHEEEEEECCCCCCCEEEEEEEEECCCEEEEEECCHH
QVKAIKSLALNILQRYPDISPTNVVGHSDVAPGRKSDPGPLFPWQELYKEGIGAWYDDAT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHH
KKEYEKIFFEHGLPIEKEIIEKLGIYGYDVSHASHPDGLKALVRSFQMHFRPADYDGRVD
HHHHHHHHHHCCCCHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCCCEEC
VETTAILYALVKKYFNK
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYQIDYNSYRAIKGFNRRVRFLVMHYTAADFKRSIEDLAVNGKVSVHYLIPDPTEKTYID
CEEECCCCHHHHHHHCCEEEEEEEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEC
AGFKELQIFNLVDESERAWHAGVSYWAGRVNINDSSIGIENVNLAADHGDGIIFLPYNEA
CCHHHEEEEEHHCCCCHHHHHHHHHEEEEEECCCCCCCEEEEEEEEECCCEEEEEECCHH
QVKAIKSLALNILQRYPDISPTNVVGHSDVAPGRKSDPGPLFPWQELYKEGIGAWYDDAT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHH
KKEYEKIFFEHGLPIEKEIIEKLGIYGYDVSHASHPDGLKALVRSFQMHFRPADYDGRVD
HHHHHHHHHHCCCCHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCCCEEC
VETTAILYALVKKYFNK
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]