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The map label for this gene is aefA [H]

Identifier: 34498417

GI number: 34498417

Start: 3241035

End: 3242324

Strand: Reverse

Name: aefA [H]

Synonym: CV_2962

Alternate gene names: 34498417

Gene position: 3242324-3241035 (Counterclockwise)

Preceding gene: 34498418

Following gene: 34498416

Centisome position: 68.24

GC content: 63.72

Gene sequence:

>1290_bases
TTGCTGTTCATCCGCAAGGAAATGAATTTCTTCGCGCTGCTTGACGCGCTGCAGACCAAGAACGGTCTGATCGAGCTGGC
GCTGGGCCTGCTGTGCGTGATCGCCGCCTATGCGCTGGCGCGCCGGCTGTACCGCTACTGGTTCGCCCATCACCCGGAGC
GTTACGACCAGTTCCTGCCCTATACCGGCTTCCGGCTGGTGCTGCCGGTGTCGGCCCAGGTGCTGGTGGTGCTGGCCAGC
TTTACCTGGGGCCGGCTGGAGCACGCGCCGATGCTGGTGCTGCACATCCTGAGCGCGCTGCTGTTCTGGCTGGCGGTGAT
CCGTCTGTGCACGGCGGTGGTGCGCCAGGCTTTTCCGCACAGCCGTTTCGAACGGCATTCCGAGCACTTCCTGGCCACGG
CCTTGTGGCTGGGCTTCGTCAGTTGGACCATAGGCTTCGACGTGGCGGTGATGGACTGGCTGGAGTCGATCTCCTTCCAC
GTCGGAAAGACCAGGCTGGACTTGCTGACCATCCTCAACGGCCTGCTGTGGGTGACGGTGATCGTGGTGGTGGCGCTGTG
GGCCAGCCGCCTGATCGAAGCCCGCATCATGAAGCTGAAGCATCTGGACTCCAATCTGCGCATCGTGTTCGCCAAGCTGA
CGCGCACCGGATTGGTGGTGGCCGCGGTGCTGGTCGCGCTGCCGGTGGTGGGAATAGACTTGACCGTGCTGTCGGTGTTC
GGCGGCGCGGTCGGTATCGGCCTGGGCTTCGGCCTGCAGAAGATCGCCAGCAACTTCGTGTCCGGCTTCATCATCCTGCT
CGACCATTCCATCCGCATCGGCGACCGGCTGATGGTGGACAACCGCGTCGGCTACGTGACCAAGATGACCTCGCGCTACG
TGGTGCTGAAAGGCGCCGACGGCACCGAGGCGCTGATTCCTAACGATGCCCTGATCTCCAACACGGTGATCAACCAGTCC
TACTCCGACCGCAATATGTGGACCAGCCTGCCGGTGCAGGTGGCCTACGGCACCGACCTGGACCTGGCGCTCAGCCTGCT
CAAGCAGGCGTCCGACCATCCGCGCGTGCTGAAGAATCCGGGCGCCAACGCCTTCGTCACCCTGTTCGCCGACAGCGGCA
TCAACCTGGAGCTGGGCTTCTGGGTGGCGGATCCGGAAAACGGCTTCATGGGCCTGAAGTCGGACATCAACCTGGCCATC
TGGCGCTTGTTCAAGCAGCACAATATCGAGATCCCGTTCCCGCAGCGGGAAGTGCGCATCGTCGGCGACAAGCCGGCGCA
GCAGGGCTGA

Upstream 100 bases:

>100_bases
CCAGCGGGTGCTGCGCCTGAACGAAGGCCGGCTGGCGGTGTCGGATACCGTTTCTGCCTGACGACGGCCGAGCGCCGCGT
TTTGACCAAGGAGTCGCACA

Downstream 100 bases:

>100_bases
GGAGATATCATGAGCGCGAAACGGCTGGCCATCATCGACGATGACGAGGCGGTGCGGCAGTCGCTGTTGTGGCTGCTGGA
CGGCCAGGGTTACCGGGCCG

Product: transmembrane protein

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MLFIRKEMNFFALLDALQTKNGLIELALGLLCVIAAYALARRLYRYWFAHHPERYDQFLPYTGFRLVLPVSAQVLVVLAS
FTWGRLEHAPMLVLHILSALLFWLAVIRLCTAVVRQAFPHSRFERHSEHFLATALWLGFVSWTIGFDVAVMDWLESISFH
VGKTRLDLLTILNGLLWVTVIVVVALWASRLIEARIMKLKHLDSNLRIVFAKLTRTGLVVAAVLVALPVVGIDLTVLSVF
GGAVGIGLGFGLQKIASNFVSGFIILLDHSIRIGDRLMVDNRVGYVTKMTSRYVVLKGADGTEALIPNDALISNTVINQS
YSDRNMWTSLPVQVAYGTDLDLALSLLKQASDHPRVLKNPGANAFVTLFADSGINLELGFWVADPENGFMGLKSDINLAI
WRLFKQHNIEIPFPQREVRIVGDKPAQQG

Sequences:

>Translated_429_residues
MLFIRKEMNFFALLDALQTKNGLIELALGLLCVIAAYALARRLYRYWFAHHPERYDQFLPYTGFRLVLPVSAQVLVVLAS
FTWGRLEHAPMLVLHILSALLFWLAVIRLCTAVVRQAFPHSRFERHSEHFLATALWLGFVSWTIGFDVAVMDWLESISFH
VGKTRLDLLTILNGLLWVTVIVVVALWASRLIEARIMKLKHLDSNLRIVFAKLTRTGLVVAAVLVALPVVGIDLTVLSVF
GGAVGIGLGFGLQKIASNFVSGFIILLDHSIRIGDRLMVDNRVGYVTKMTSRYVVLKGADGTEALIPNDALISNTVINQS
YSDRNMWTSLPVQVAYGTDLDLALSLLKQASDHPRVLKNPGANAFVTLFADSGINLELGFWVADPENGFMGLKSDINLAI
WRLFKQHNIEIPFPQREVRIVGDKPAQQG
>Mature_429_residues
MLFIRKEMNFFALLDALQTKNGLIELALGLLCVIAAYALARRLYRYWFAHHPERYDQFLPYTGFRLVLPVSAQVLVVLAS
FTWGRLEHAPMLVLHILSALLFWLAVIRLCTAVVRQAFPHSRFERHSEHFLATALWLGFVSWTIGFDVAVMDWLESISFH
VGKTRLDLLTILNGLLWVTVIVVVALWASRLIEARIMKLKHLDSNLRIVFAKLTRTGLVVAAVLVALPVVGIDLTVLSVF
GGAVGIGLGFGLQKIASNFVSGFIILLDHSIRIGDRLMVDNRVGYVTKMTSRYVVLKGADGTEALIPNDALISNTVINQS
YSDRNMWTSLPVQVAYGTDLDLALSLLKQASDHPRVLKNPGANAFVTLFADSGINLELGFWVADPENGFMGLKSDINLAI
WRLFKQHNIEIPFPQREVRIVGDKPAQQG

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=300, Percent_Identity=31, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI2367355, Length=236, Percent_Identity=33.4745762711864, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789291, Length=257, Percent_Identity=26.4591439688716, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787591, Length=332, Percent_Identity=21.6867469879518, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 47900; Mature: 47900

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFIRKEMNFFALLDALQTKNGLIELALGLLCVIAAYALARRLYRYWFAHHPERYDQFLP
CEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCC
YTGFRLVLPVSAQVLVVLASFTWGRLEHAPMLVLHILSALLFWLAVIRLCTAVVRQAFPH
CCCCEEEECCCHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SRFERHSEHFLATALWLGFVSWTIGFDVAVMDWLESISFHVGKTRLDLLTILNGLLWVTV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IVVVALWASRLIEARIMKLKHLDSNLRIVFAKLTRTGLVVAAVLVALPVVGIDLTVLSVF
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGAVGIGLGFGLQKIASNFVSGFIILLDHSIRIGDRLMVDNRVGYVTKMTSRYVVLKGAD
HHHHHHHHHHHHHHHHHHHHHHEEEEEECCEECCCEEEECCCCCCEEEECCCEEEEECCC
GTEALIPNDALISNTVINQSYSDRNMWTSLPVQVAYGTDLDLALSLLKQASDHPRVLKNP
CCCCCCCCCHHHHHHHHCCCCCCCCCEECCCEEEEECCCHHHHHHHHHHCCCCCCHHCCC
GANAFVTLFADSGINLELGFWVADPENGFMGLKSDINLAIWRLFKQHNIEIPFPQREVRI
CCCEEEEEEECCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEE
VGDKPAQQG
ECCCCCCCC
>Mature Secondary Structure
MLFIRKEMNFFALLDALQTKNGLIELALGLLCVIAAYALARRLYRYWFAHHPERYDQFLP
CEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCC
YTGFRLVLPVSAQVLVVLASFTWGRLEHAPMLVLHILSALLFWLAVIRLCTAVVRQAFPH
CCCCEEEECCCHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SRFERHSEHFLATALWLGFVSWTIGFDVAVMDWLESISFHVGKTRLDLLTILNGLLWVTV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IVVVALWASRLIEARIMKLKHLDSNLRIVFAKLTRTGLVVAAVLVALPVVGIDLTVLSVF
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGAVGIGLGFGLQKIASNFVSGFIILLDHSIRIGDRLMVDNRVGYVTKMTSRYVVLKGAD
HHHHHHHHHHHHHHHHHHHHHHEEEEEECCEECCCEEEECCCCCCEEEECCCEEEEECCC
GTEALIPNDALISNTVINQSYSDRNMWTSLPVQVAYGTDLDLALSLLKQASDHPRVLKNP
CCCCCCCCCHHHHHHHHCCCCCCCCCEECCCEEEEECCCHHHHHHHHHHCCCCCCHHCCC
GANAFVTLFADSGINLELGFWVADPENGFMGLKSDINLAIWRLFKQHNIEIPFPQREVRI
CCCEEEEEEECCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEE
VGDKPAQQG
ECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]