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The map label for this gene is dmlR [H]

Identifier: 34498252

GI number: 34498252

Start: 3031183

End: 3032076

Strand: Direct

Name: dmlR [H]

Synonym: CV_2797

Alternate gene names: 34498252

Gene position: 3031183-3032076 (Clockwise)

Preceding gene: 34498251

Following gene: 34498260

Centisome position: 63.8

GC content: 69.91

Gene sequence:

>894_bases
ATGCCGTTCGACCGTCTGGCCGACCTGCGCCTGTTTCAGGACGCCTCCCAGCTTGGCAGCTTTTCCGCCGCCGGCCGCAA
GCACGGCCTGTCCCCCGCCGCCGCCAGCGCCTGCATCCAGCGCATGGAGGCGGCCTTGAACGCCCGCCTGTTCCAGCGCA
CCACCCGCCGCCTGCGCCTGACCGAGGCCGGGGAGACCTATCTCGCCTACTGCCGGCAGGCGCTGGAATTGCTGGAGGAA
GGCGAACACCGGCTGCAGCAGGCGCAACAGGAATTGAGCGGCGTGATCCGCCTGTCCGCGCCGTCCGATCTGGGCCGCAA
CCGGCTGCTGGATTGCCTGGACCGTTTCGGCGCCGAGCATCCCGCCGTGCACTTCTCGCTGTCGCTCAGCGACACTCCGG
CCGACCTGATCGGCGACGACATCGACCTCGCCATCCGCTACGGCCAGCCGGCCGACAGCAGCCTGGTGGCGCGCCAGCTG
GCCGCCAGCCGCCGCGTGGTCTGCGCCGCGCCGTCGCTGCTGGAAAAGCTGGGCCAACCATCGCATCCCCAAGACCTCGC
CGGCCTGCCCACGCTGACGCTGACCACCGGCCATGGCCCGATGAATGAATGGCGTTACCGGGACAAGGGGGAAATCCGCT
CGCTGCGGCTGGAGCGCGTGCGCCAGAGCAATGACGGAGAGGTGCTGCGCCGCTGGGCGGTGCAGGGTCACGGCTTCGCC
TACAAGTCGCGGCTGGACATCGCCGACGACCTCGCCGCCGGCAGGCTGACGACGGTGCTGGACGACTACTTCATCGAGCC
CGCGCCGCTGCACCTGCTCTACCCCGGCCACCGGCTGCAGCCGGCGCGGATACGGCGGCTGATAGATTTCCTGCTGGATC
AGTTCGCCGCCTAG

Upstream 100 bases:

>100_bases
TGGCTGCTGGCCAACACCGAAACGCTGTAAACTCAGGCGATACCCGTCGACAGGCCATGCCGCTGCGGCATGGCCTGTTT
CGTTTTGCAAGGAATGACCG

Downstream 100 bases:

>100_bases
AGTGGCTAACAACACTCAATTTTACGGCAGAGACAAGGCGCGCCGACGCAGACAGTACAAGCAGTACGGCAAGCCAGCGC
AGCATCAGGGGTTTGGTGGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MPFDRLADLRLFQDASQLGSFSAAGRKHGLSPAAASACIQRMEAALNARLFQRTTRRLRLTEAGETYLAYCRQALELLEE
GEHRLQQAQQELSGVIRLSAPSDLGRNRLLDCLDRFGAEHPAVHFSLSLSDTPADLIGDDIDLAIRYGQPADSSLVARQL
AASRRVVCAAPSLLEKLGQPSHPQDLAGLPTLTLTTGHGPMNEWRYRDKGEIRSLRLERVRQSNDGEVLRRWAVQGHGFA
YKSRLDIADDLAAGRLTTVLDDYFIEPAPLHLLYPGHRLQPARIRRLIDFLLDQFAA

Sequences:

>Translated_297_residues
MPFDRLADLRLFQDASQLGSFSAAGRKHGLSPAAASACIQRMEAALNARLFQRTTRRLRLTEAGETYLAYCRQALELLEE
GEHRLQQAQQELSGVIRLSAPSDLGRNRLLDCLDRFGAEHPAVHFSLSLSDTPADLIGDDIDLAIRYGQPADSSLVARQL
AASRRVVCAAPSLLEKLGQPSHPQDLAGLPTLTLTTGHGPMNEWRYRDKGEIRSLRLERVRQSNDGEVLRRWAVQGHGFA
YKSRLDIADDLAAGRLTTVLDDYFIEPAPLHLLYPGHRLQPARIRRLIDFLLDQFAA
>Mature_296_residues
PFDRLADLRLFQDASQLGSFSAAGRKHGLSPAAASACIQRMEAALNARLFQRTTRRLRLTEAGETYLAYCRQALELLEEG
EHRLQQAQQELSGVIRLSAPSDLGRNRLLDCLDRFGAEHPAVHFSLSLSDTPADLIGDDIDLAIRYGQPADSSLVARQLA
ASRRVVCAAPSLLEKLGQPSHPQDLAGLPTLTLTTGHGPMNEWRYRDKGEIRSLRLERVRQSNDGEVLRRWAVQGHGFAY
KSRLDIADDLAAGRLTTVLDDYFIEPAPLHLLYPGHRLQPARIRRLIDFLLDQFAA

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=291, Percent_Identity=30.5841924398625, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI1789639, Length=295, Percent_Identity=30.5084745762712, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=30.5841924398625, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=31.1188811188811, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1789440, Length=284, Percent_Identity=27.4647887323944, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787128, Length=287, Percent_Identity=25.4355400696864, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1786448, Length=295, Percent_Identity=28.4745762711864, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1787589, Length=280, Percent_Identity=25.3571428571429, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789173, Length=299, Percent_Identity=25.4180602006689, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790208, Length=173, Percent_Identity=33.5260115606936, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787601, Length=172, Percent_Identity=26.1627906976744, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788481, Length=257, Percent_Identity=27.2373540856031, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33081; Mature: 32950

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS50931 HTH_LYSR ; PS00152 ATPASE_ALPHA_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFDRLADLRLFQDASQLGSFSAAGRKHGLSPAAASACIQRMEAALNARLFQRTTRRLRL
CCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEAGETYLAYCRQALELLEEGEHRLQQAQQELSGVIRLSAPSDLGRNRLLDCLDRFGAEH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHCCCC
PAVHFSLSLSDTPADLIGDDIDLAIRYGQPADSSLVARQLAASRRVVCAAPSLLEKLGQP
CCEEEEEECCCCCHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCEEECCHHHHHHCCCC
SHPQDLAGLPTLTLTTGHGPMNEWRYRDKGEIRSLRLERVRQSNDGEVLRRWAVQGHGFA
CCCCHHCCCCEEEEECCCCCCHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEE
YKSRLDIADDLAAGRLTTVLDDYFIEPAPLHLLYPGHRLQPARIRRLIDFLLDQFAA
ECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PFDRLADLRLFQDASQLGSFSAAGRKHGLSPAAASACIQRMEAALNARLFQRTTRRLRL
CHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEAGETYLAYCRQALELLEEGEHRLQQAQQELSGVIRLSAPSDLGRNRLLDCLDRFGAEH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHCCCC
PAVHFSLSLSDTPADLIGDDIDLAIRYGQPADSSLVARQLAASRRVVCAAPSLLEKLGQP
CCEEEEEECCCCCHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCEEECCHHHHHHCCCC
SHPQDLAGLPTLTLTTGHGPMNEWRYRDKGEIRSLRLERVRQSNDGEVLRRWAVQGHGFA
CCCCHHCCCCEEEEECCCCCCHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEE
YKSRLDIADDLAAGRLTTVLDDYFIEPAPLHLLYPGHRLQPARIRRLIDFLLDQFAA
ECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]