Click here to switch to the map view.

The map label for this gene is nuoB1

Identifier: 34498210

GI number: 34498210

Start: 2991365

End: 2991880

Strand: Direct

Name: nuoB1

Synonym: CV_2755

Alternate gene names: 34498210

Gene position: 2991365-2991880 (Clockwise)

Preceding gene: 34498209

Following gene: 34498212

Centisome position: 62.96

GC content: 68.22

Gene sequence:

>516_bases
ATGGACAGCCCCTCCCTGGAAAAAGACGGATTCCTGCTCACCACCCTAGACGGCCTGTTCGGCAGGGCCCAGAGCGGCAG
CTTGTGGTATCTGTCGTTCGGCCTCGCCTGCTGCGCGGTGGAAATGATGCACGCGGCGGCCGCGCGCTACGACATGGACC
GCTTCGGCTGGATACCGCGCGCCACGCCGCGCCAGGCCGACCTGATGATCGTGGCCGGCACGCTGACCAACAAGATGGCG
CCGGCGATGCGCAAGGTCTACGACCAGATGTCGGAGCCGCGCTACGTGCTGTCGATGGGCTCCTGCGCCAACGGCGGCGG
CTACTACCATTACAGCTACGCGGTGGTGCGCGGCTGCGACCGCATCGTGCCGGTGGACGTCTACGTGCCCGGCTGCCCGC
CCACCGCCGAGGCGCTGCTGTACGGCCTGATGCAGCTGCAGGCCAAGATACGCCGCGACGACACCGCCAGCCGGCGCGAG
CTGCTGGACCGCTCGCCAAACCGGCAGCCTAACTGA

Upstream 100 bases:

>100_bases
CGGGAAATGCGGGTAGAAGACACAGGGGATGTCGTCTCGGTGCAAGACGCCTTCTGAGCAAGTGGCGCAGCATGGGTCCG
TTGCGTAATGGAGACCTTAG

Downstream 100 bases:

>100_bases
ATCAGTCTTCGCGCAGCTCGAAATCGTGGGTGATGTCGGCGGTCTTGGCCAGCATAATGGACGCGGAACAGTATTTCTCC
GCCGACAGCTTGATCGCCCG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B 2; NDH-1 subunit B 2

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MDSPSLEKDGFLLTTLDGLFGRAQSGSLWYLSFGLACCAVEMMHAAAARYDMDRFGWIPRATPRQADLMIVAGTLTNKMA
PAMRKVYDQMSEPRYVLSMGSCANGGGYYHYSYAVVRGCDRIVPVDVYVPGCPPTAEALLYGLMQLQAKIRRDDTASRRE
LLDRSPNRQPN

Sequences:

>Translated_171_residues
MDSPSLEKDGFLLTTLDGLFGRAQSGSLWYLSFGLACCAVEMMHAAAARYDMDRFGWIPRATPRQADLMIVAGTLTNKMA
PAMRKVYDQMSEPRYVLSMGSCANGGGYYHYSYAVVRGCDRIVPVDVYVPGCPPTAEALLYGLMQLQAKIRRDDTASRRE
LLDRSPNRQPN
>Mature_171_residues
MDSPSLEKDGFLLTTLDGLFGRAQSGSLWYLSFGLACCAVEMMHAAAARYDMDRFGWIPRATPRQADLMIVAGTLTNKMA
PAMRKVYDQMSEPRYVLSMGSCANGGGYYHYSYAVVRGCDRIVPVDVYVPGCPPTAEALLYGLMQLQAKIRRDDTASRRE
LLDRSPNRQPN

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=143, Percent_Identity=76.2237762237762, Blast_Score=239, Evalue=9e-64,
Organism=Escherichia coli, GI1788624, Length=145, Percent_Identity=48.9655172413793, Blast_Score=151, Evalue=2e-38,
Organism=Escherichia coli, GI1789074, Length=134, Percent_Identity=37.3134328358209, Blast_Score=96, Evalue=9e-22,
Organism=Escherichia coli, GI1788834, Length=126, Percent_Identity=38.8888888888889, Blast_Score=91, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=150, Percent_Identity=68.6666666666667, Blast_Score=230, Evalue=3e-61,
Organism=Drosophila melanogaster, GI18859983, Length=143, Percent_Identity=74.8251748251748, Blast_Score=238, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24642371, Length=143, Percent_Identity=74.8251748251748, Blast_Score=238, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24651058, Length=140, Percent_Identity=76.4285714285714, Blast_Score=233, Evalue=6e-62,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB2_CHRVO (Q7NUE5)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_902425.1
- HSSP:   Q56218
- ProteinModelPortal:   Q7NUE5
- SMR:   Q7NUE5
- GeneID:   2548493
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_2755
- NMPDR:   fig|243365.1.peg.2755
- HOGENOM:   HBG553221
- OMA:   FIATSMS
- ProtClustDB:   PRK06411
- BioCyc:   CVIO243365:CV_2755-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 19000; Mature: 19000

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSPSLEKDGFLLTTLDGLFGRAQSGSLWYLSFGLACCAVEMMHAAAARYDMDRFGWIPR
CCCCCCCCCCEEEEEHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCC
ATPRQADLMIVAGTLTNKMAPAMRKVYDQMSEPRYVLSMGSCANGGGYYHYSYAVVRGCD
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHCCC
RIVPVDVYVPGCPPTAEALLYGLMQLQAKIRRDDTASRRELLDRSPNRQPN
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDSPSLEKDGFLLTTLDGLFGRAQSGSLWYLSFGLACCAVEMMHAAAARYDMDRFGWIPR
CCCCCCCCCCEEEEEHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCC
ATPRQADLMIVAGTLTNKMAPAMRKVYDQMSEPRYVLSMGSCANGGGYYHYSYAVVRGCD
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHCCC
RIVPVDVYVPGCPPTAEALLYGLMQLQAKIRRDDTASRRELLDRSPNRQPN
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 14500782