| Definition | Chromobacterium violaceum ATCC 12472 chromosome, complete genome. |
|---|---|
| Accession | NC_005085 |
| Length | 4,751,080 |
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The map label for this gene is pbpB [H]
Identifier: 34498017
GI number: 34498017
Start: 2762807
End: 2765011
Strand: Direct
Name: pbpB [H]
Synonym: CV_2562
Alternate gene names: 34498017
Gene position: 2762807-2765011 (Clockwise)
Preceding gene: 34498015
Following gene: 34498021
Centisome position: 58.15
GC content: 71.34
Gene sequence:
>2205_bases ATGCGTCTTGCGCGTGACTTTGCCCCCACTACTTGCAACAGAACAAAGCCCCCAGTGACCCGCTACAACGCCAGCCTCCT CATTCTCGCCCTGCTCTCCTCACCCGCCTGGGCCCAGCCCACGTTCAAAGAGGTGAAGGCCGCCTGGCAGCCATCCGACG TGACGCTGCTGGACCGCCACGGCCAGGTGCTGCAGCGGCTGCGCGTCGACAAGCAGGCGCGTCGGCAGGATTGGGTCGGC CTGGCGGACACCTCCCCCGCCTTCCGCCAAGCGCTGGTGCTATCCGAGGACAAGCGCTTCTACCAGCACAGCGGCGTGGA CTGGTCAGGCGCGGCGGCGGCTGCCTGGGCCAATCTGTGGAACACCCGCACCCGCGGCGCGTCCACGCTGACCATGCAGC TGGCCGGCCTGCTGGACGATGATCTGAAGGTAGGCAAGGGGGGGCGCAGCCTGTGGCAGAAGATGGGCCAAACCTGGTCC GCCGCCTGGCTGGAGCAGCACTGGACCAAGGCGGAGATTCTGGAGGCCTACCTGAACCTGGTGGGTTTCCGCGGCGAACT GGTGGGCCTGTCCTCGCTGTCGTCGACGCTGTTCGGCAAGCTGCCGTCCGGACTGAACCTGGAAGAATCGGCGATCGCGG TGGCGCTGATCCGCGCGCCCAACGCCGCGCCGGACAAGGTAGCCGGCCGCGCCTGCCGCATCCTGCAGGACATGGGCCGC AAGCCGGACTGCGCGGCGCTGGGCGTGCGCGCCACCCAGGCCCTGGCCCGCAGCCGAGCCGCCAATCCGCTGGCCGAGCA GGACGCGCCGCATTTCGCCCAGAAGCTGCAGCAGGCGCGCCGCTTCGCCGCCGGCAGCCGGGTGGCGACCACGCTGGACC TGCCGCTGCAACGTTTCGCCGCCGCCGCGCTGCGACGCCACCTGTCCGCGCTGTCGCAGCGCAATGTGCAGGACGGCGCG CTGGTGGTGCTGGACAACCAGAGCGGCGACATCCTGGCCTGGGTGGGCTCCAGCGGCCGCGGCCTGTCCAACGCCGCCCA GGTGGACGGCGTCACCGCGCCGCGCCAGGCCGGCTCCACGCTCAAGCCCTTCCTGTACCAGCAGGCGCTGGAAAAGCATT ATCTGACCGCGGCCTCGCTGCTGGACGACAGCCCGCTGGACCTGCAGACCGGCAGCGGCCTGTACGCGCCGCAAAATTAC TCCAAGGACTTCAAGGGGCTGGTGCCGGTGCGCAGCGCGCTGGCGTCATCGCTGAACATCCCGGCGGTGCGGACGATTGA GATGGTCACGCCCAACGCCGCGCGCGACCGGCTGGTGAAGCTGGGCTTCTCCACCCTGACCGAGGACGGCGACTACTACG GCTACAGCCTGGCGCTGGGCGCCGCCGACATCCGCCTGCTGGACCTGGCCAACGCCTACCGCACGCTGGCCAACCAGGGC CTGTCCAGCCCGCCGCGCTGGACGCTGACCGACGCCAAGCCCAAACCGGCGCGGCTGCTCGACGCCGCCAGCAGCTTCAT CATCGCCGACATCCTGTCCGACCGCACCGCGCGCGCGCTCACCTTCGGCCTGGAAAGCGCGCTGTCCACCCGGTACTGGA GCGCGGTGAAGACCGGCACCAGCAAGGACATGCGCGACAATTGGGCGGTGGGCTTCTCACGCCGCTTCACGGTGGCGGCC TGGGTAGGCAATGCCAGCGGCGAGCCGATGTGGGACGTATCCGGCATGCACGGCGCGGCGCCGGTCTGGCAGACCGTGCT GGACCGCGCCCAGCAATCGGCCGCGCCCCTGCCGCCGCCCGCGCCGCCGGCCGGCCTGGTGCGCCAGCGGGTCCGCTATC AGGGCGACGTGGCCGCGCCCCGGACCGAGTGGTTCCTGGCCGGCACCCAGCGCGCCCTGATCGTGCCGCAGAAAGGCGCC GCCGCCGACCTGACCCCGGGCATCGCCGCGCCGGTGGACGGCAGCATCTTCGCGCTCGACCCGGACATTCCGCCGGCCAA CCAGCGCATGGTGCTGCGCGCGCGCGGCGTGGCCAAGCCGCAATGGTGGCTGGACGGCAAGCGGCTGGGAAGCGGAGCGG AGCTGGCCTGGTTCCCCTGGCCGGGCCGGCATCGGCTGGAGCTGCGCGGCGGCGACGGCAAGGTGGTGCAGGCGGTGAAA TTCGAGGTGCGCGGCGCTTCGCTGCGGCCCGGCAGGCCGCGTTGA
Upstream 100 bases:
>100_bases ACCCCCTGTCCATGAAAGAACGTGCAAGAAACGAAATACTCAAAGCATAATATAGCCATGAAAGATCTGCCCGCACAGCA AACATCAATAACGCTATACA
Downstream 100 bases:
>100_bases AGCGCCGCGTCCCCTCAGACTAGCCGGCCGCGGCCTTGCGCCGCTGCAGCGACTGCCACAACTGGTCCGCTCGCCGCGCG GCGTTGGCTTTCTGCTGCGC
Product: penicillin-binding protein 2
Products: NA
Alternate protein names: PBP-1c; PBP1c; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Transpeptidase-like module [H]
Number of amino acids: Translated: 734; Mature: 734
Protein sequence:
>734_residues MRLARDFAPTTCNRTKPPVTRYNASLLILALLSSPAWAQPTFKEVKAAWQPSDVTLLDRHGQVLQRLRVDKQARRQDWVG LADTSPAFRQALVLSEDKRFYQHSGVDWSGAAAAAWANLWNTRTRGASTLTMQLAGLLDDDLKVGKGGRSLWQKMGQTWS AAWLEQHWTKAEILEAYLNLVGFRGELVGLSSLSSTLFGKLPSGLNLEESAIAVALIRAPNAAPDKVAGRACRILQDMGR KPDCAALGVRATQALARSRAANPLAEQDAPHFAQKLQQARRFAAGSRVATTLDLPLQRFAAAALRRHLSALSQRNVQDGA LVVLDNQSGDILAWVGSSGRGLSNAAQVDGVTAPRQAGSTLKPFLYQQALEKHYLTAASLLDDSPLDLQTGSGLYAPQNY SKDFKGLVPVRSALASSLNIPAVRTIEMVTPNAARDRLVKLGFSTLTEDGDYYGYSLALGAADIRLLDLANAYRTLANQG LSSPPRWTLTDAKPKPARLLDAASSFIIADILSDRTARALTFGLESALSTRYWSAVKTGTSKDMRDNWAVGFSRRFTVAA WVGNASGEPMWDVSGMHGAAPVWQTVLDRAQQSAAPLPPPAPPAGLVRQRVRYQGDVAAPRTEWFLAGTQRALIVPQKGA AADLTPGIAAPVDGSIFALDPDIPPANQRMVLRARGVAKPQWWLDGKRLGSGAELAWFPWPGRHRLELRGGDGKVVQAVK FEVRGASLRPGRPR
Sequences:
>Translated_734_residues MRLARDFAPTTCNRTKPPVTRYNASLLILALLSSPAWAQPTFKEVKAAWQPSDVTLLDRHGQVLQRLRVDKQARRQDWVG LADTSPAFRQALVLSEDKRFYQHSGVDWSGAAAAAWANLWNTRTRGASTLTMQLAGLLDDDLKVGKGGRSLWQKMGQTWS AAWLEQHWTKAEILEAYLNLVGFRGELVGLSSLSSTLFGKLPSGLNLEESAIAVALIRAPNAAPDKVAGRACRILQDMGR KPDCAALGVRATQALARSRAANPLAEQDAPHFAQKLQQARRFAAGSRVATTLDLPLQRFAAAALRRHLSALSQRNVQDGA LVVLDNQSGDILAWVGSSGRGLSNAAQVDGVTAPRQAGSTLKPFLYQQALEKHYLTAASLLDDSPLDLQTGSGLYAPQNY SKDFKGLVPVRSALASSLNIPAVRTIEMVTPNAARDRLVKLGFSTLTEDGDYYGYSLALGAADIRLLDLANAYRTLANQG LSSPPRWTLTDAKPKPARLLDAASSFIIADILSDRTARALTFGLESALSTRYWSAVKTGTSKDMRDNWAVGFSRRFTVAA WVGNASGEPMWDVSGMHGAAPVWQTVLDRAQQSAAPLPPPAPPAGLVRQRVRYQGDVAAPRTEWFLAGTQRALIVPQKGA AADLTPGIAAPVDGSIFALDPDIPPANQRMVLRARGVAKPQWWLDGKRLGSGAELAWFPWPGRHRLELRGGDGKVVQAVK FEVRGASLRPGRPR >Mature_734_residues MRLARDFAPTTCNRTKPPVTRYNASLLILALLSSPAWAQPTFKEVKAAWQPSDVTLLDRHGQVLQRLRVDKQARRQDWVG LADTSPAFRQALVLSEDKRFYQHSGVDWSGAAAAAWANLWNTRTRGASTLTMQLAGLLDDDLKVGKGGRSLWQKMGQTWS AAWLEQHWTKAEILEAYLNLVGFRGELVGLSSLSSTLFGKLPSGLNLEESAIAVALIRAPNAAPDKVAGRACRILQDMGR KPDCAALGVRATQALARSRAANPLAEQDAPHFAQKLQQARRFAAGSRVATTLDLPLQRFAAAALRRHLSALSQRNVQDGA LVVLDNQSGDILAWVGSSGRGLSNAAQVDGVTAPRQAGSTLKPFLYQQALEKHYLTAASLLDDSPLDLQTGSGLYAPQNY SKDFKGLVPVRSALASSLNIPAVRTIEMVTPNAARDRLVKLGFSTLTEDGDYYGYSLALGAADIRLLDLANAYRTLANQG LSSPPRWTLTDAKPKPARLLDAASSFIIADILSDRTARALTFGLESALSTRYWSAVKTGTSKDMRDNWAVGFSRRFTVAA WVGNASGEPMWDVSGMHGAAPVWQTVLDRAQQSAAPLPPPAPPAGLVRQRVRYQGDVAAPRTEWFLAGTQRALIVPQKGA AADLTPGIAAPVDGSIFALDPDIPPANQRMVLRARGVAKPQWWLDGKRLGSGAELAWFPWPGRHRLELRGGDGKVVQAVK FEVRGASLRPGRPR
Specific function: Cell wall formation. The enzyme has a penicillin- insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a transpeptidase C-terminal domain which may not be functional [H]
COG id: COG4953
COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein PbpC
Gene ontology:
Cell location: Cell inner membrane; Single-pass type II membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI1788867, Length=615, Percent_Identity=32.1951219512195, Blast_Score=214, Evalue=2e-56, Organism=Escherichia coli, GI1786343, Length=572, Percent_Identity=26.9230769230769, Blast_Score=118, Evalue=1e-27, Organism=Escherichia coli, GI87082258, Length=418, Percent_Identity=26.0765550239234, Blast_Score=89, Evalue=9e-19,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011815 - InterPro: IPR009647 - InterPro: IPR001460 [H]
Pfam domain/function: PF06832 BiPBP_C; PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: 2.4.2.-
Molecular weight: Translated: 79474; Mature: 79474
Theoretical pI: Translated: 10.69; Mature: 10.69
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 1.2 %Met (Translated Protein) 1.6 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 1.2 %Met (Mature Protein) 1.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRLARDFAPTTCNRTKPPVTRYNASLLILALLSSPAWAQPTFKEVKAAWQPSDVTLLDRH CCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCEEEHHH GQVLQRLRVDKQARRQDWVGLADTSPAFRQALVLSEDKRFYQHSGVDWSGAAAAAWANLW HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH NTRTRGASTLTMQLAGLLDDDLKVGKGGRSLWQKMGQTWSAAWLEQHWTKAEILEAYLNL HCCCCCCHHHHHHHHHHCCCCHHCCCCHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHH VGFRGELVGLSSLSSTLFGKLPSGLNLEESAIAVALIRAPNAAPDKVAGRACRILQDMGR HCCCCCEEEHHHHHHHHHHCCCCCCCCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHCCC KPDCAALGVRATQALARSRAANPLAEQDAPHFAQKLQQARRFAAGSRVATTLDLPLQRFA CCCHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHH AAALRRHLSALSQRNVQDGALVVLDNQSGDILAWVGSSGRGLSNAAQVDGVTAPRQAGST HHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCCCCHHHCCCCCCCHHCCCC LKPFLYQQALEKHYLTAASLLDDSPLDLQTGSGLYAPQNYSKDFKGLVPVRSALASSLNI HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC PAVRTIEMVTPNAARDRLVKLGFSTLTEDGDYYGYSLALGAADIRLLDLANAYRTLANQG CCEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEHHHHHHHHHHHHHCC LSSPPRWTLTDAKPKPARLLDAASSFIIADILSDRTARALTFGLESALSTRYWSAVKTGT CCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC SKDMRDNWAVGFSRRFTVAAWVGNASGEPMWDVSGMHGAAPVWQTVLDRAQQSAAPLPPP CCCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCCC APPAGLVRQRVRYQGDVAAPRTEWFLAGTQRALIVPQKGAAADLTPGIAAPVDGSIFALD CCCHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEC PDIPPANQRMVLRARGVAKPQWWLDGKRLGSGAELAWFPWPGRHRLELRGGDGKVVQAVK CCCCCCCCEEEEEECCCCCCCEECCHHHCCCCCCEEEECCCCCEEEEEECCCCCEEEEEE FEVRGASLRPGRPR EEECCCCCCCCCCC >Mature Secondary Structure MRLARDFAPTTCNRTKPPVTRYNASLLILALLSSPAWAQPTFKEVKAAWQPSDVTLLDRH CCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCEEEHHH GQVLQRLRVDKQARRQDWVGLADTSPAFRQALVLSEDKRFYQHSGVDWSGAAAAAWANLW HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH NTRTRGASTLTMQLAGLLDDDLKVGKGGRSLWQKMGQTWSAAWLEQHWTKAEILEAYLNL HCCCCCCHHHHHHHHHHCCCCHHCCCCHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHH VGFRGELVGLSSLSSTLFGKLPSGLNLEESAIAVALIRAPNAAPDKVAGRACRILQDMGR HCCCCCEEEHHHHHHHHHHCCCCCCCCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHCCC KPDCAALGVRATQALARSRAANPLAEQDAPHFAQKLQQARRFAAGSRVATTLDLPLQRFA CCCHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHH AAALRRHLSALSQRNVQDGALVVLDNQSGDILAWVGSSGRGLSNAAQVDGVTAPRQAGST HHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCCCCHHHCCCCCCCHHCCCC LKPFLYQQALEKHYLTAASLLDDSPLDLQTGSGLYAPQNYSKDFKGLVPVRSALASSLNI HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC PAVRTIEMVTPNAARDRLVKLGFSTLTEDGDYYGYSLALGAADIRLLDLANAYRTLANQG CCEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEHHHHHHHHHHHHHCC LSSPPRWTLTDAKPKPARLLDAASSFIIADILSDRTARALTFGLESALSTRYWSAVKTGT CCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC SKDMRDNWAVGFSRRFTVAAWVGNASGEPMWDVSGMHGAAPVWQTVLDRAQQSAAPLPPP CCCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCCC APPAGLVRQRVRYQGDVAAPRTEWFLAGTQRALIVPQKGAAADLTPGIAAPVDGSIFALD CCCHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEC PDIPPANQRMVLRARGVAKPQWWLDGKRLGSGAELAWFPWPGRHRLELRGGDGKVVQAVK CCCCCCCCEEEEEECCCCCCCEECCHHHCCCCCCEEEECCCCCEEEEEECCCCCEEEEEE FEVRGASLRPGRPR EEECCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 10542235; 9205837; 9278503; 9841666 [H]