Click here to switch to the map view.

The map label for this gene is braC [H]

Identifier: 34497936

GI number: 34497936

Start: 2671678

End: 2672883

Strand: Direct

Name: braC [H]

Synonym: CV_2481

Alternate gene names: 34497936

Gene position: 2671678-2672883 (Clockwise)

Preceding gene: 34497935

Following gene: 34497937

Centisome position: 56.23

GC content: 63.43

Gene sequence:

>1206_bases
ATGAACAAATATGCTCGCTATAGCCTGATCGCCACCGCCGTCATCGCCATGAACGCCTGCAGCAAGCAGGAACAGCCGGC
ATCTGGCGGCCAGGCCGCCTCCGCTCCCAGCCAGGCCGCCGCAGGCGGCGGCGAAACCGTGGTCAAGATCGGCCAGGTTT
CTCCGATGTCCGGCCCGATTTCGCACCTGGGCAAGGACAACGAGTACGGCGCCAAGCTCGCCATCGAGGATCTGAACGCC
AAGGGCGTGGAGATCGGCGGCAAGAAGGTGAAATTCGAACTGGTGTCCGAGGACGACCAGGGCGACCCGAAAATCGGCAC
CCAGGTCGCCCAGCGCCTGGTGGACGCGGGCGTGGTCGGCGTGGTAGGCCACCTGAACTCCGGCACCACCATCCCGGCCT
CCAAGATTTACTCCGACGCCGGCCTGCCGCAGATTTCCCCGTCCGCCACCAACCCTGACTACACCAAGCAGGGCTTCAAG
ACCACCTTCCGCGTAATCGCCAACGACGTGCAGCAGGGCAAGGCGCTGGGCGAATTCGCCGCCGGCACGCTGAACGCCAA
GAAAATCGCCATCATCGACGACCGCACCGCTTACGGCCAGGGCCTGGCCGACCAGTTCGAGGGCGCGGTCAAGGCCAAGG
GCGCGCAGGTGGTGAAACGCGAGTTCACCAACAACACCGCCACCGACTTCAACGCCATTCTCACCTCGATCAAGGCCACC
AACCCGGATCTGGTGTTCTACGGCGGCATGGACGCGCAGGCGGGCCCGCTGGCCAAGCAGATGCAGCGCCTCGGCATCAA
GGCCAAGCTGATGGGCGGCGACGGCTGGCAAACCCCGGAATTCGTCAAGCTGGCCGGCGACGCCTCCGAAGGCCAGTACG
CATCCAGCTGCGGCATTTCCCGCGACAAGATGCCGGGCTTCAAGGCCTTCGACGAGAAGTTCAAGAAGGAATTCAACACC
GACGTGCAGATCTACGCGCCGTTCGAATACGACGCCGTGATGGTGCTGGTGGACGCGATGAAGCGCGCCAAGTCCTACGA
TCCGAAGGCTTACCTGCCTGAAGTCGGCAAGACCGATTACCAGGGCGTGACCGGCAAGATCGCCTTTGACGACAAGGGCG
ACATCAAAAACGGCGCCGTCACCGTGTACCAGGTGAAGAACGGCAAGTGGGAAGTGGTGTCCACCGTGGGCGGCGAAGCC
AAGTAA

Upstream 100 bases:

>100_bases
CTCAACATAATCGATCGCAGCTACCAGCCTCTTAACAGTGTTGTTGGTTTGCCAAAGAGATCATCAAAGTCTACGCAGAT
AAGATTCAGGGGAACGCTCA

Downstream 100 bases:

>100_bases
ACGCCACGCCGAATGCACCGGCGATGACAGGGGCGCTTCGGCGCCCCTTTTCGCTCCACCGAGCCTTCCCCGCTGTAGAT
ACGACTCTCGCAATCCGCAA

Product: amino acid ABC transporter

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MNKYARYSLIATAVIAMNACSKQEQPASGGQAASAPSQAAAGGGETVVKIGQVSPMSGPISHLGKDNEYGAKLAIEDLNA
KGVEIGGKKVKFELVSEDDQGDPKIGTQVAQRLVDAGVVGVVGHLNSGTTIPASKIYSDAGLPQISPSATNPDYTKQGFK
TTFRVIANDVQQGKALGEFAAGTLNAKKIAIIDDRTAYGQGLADQFEGAVKAKGAQVVKREFTNNTATDFNAILTSIKAT
NPDLVFYGGMDAQAGPLAKQMQRLGIKAKLMGGDGWQTPEFVKLAGDASEGQYASSCGISRDKMPGFKAFDEKFKKEFNT
DVQIYAPFEYDAVMVLVDAMKRAKSYDPKAYLPEVGKTDYQGVTGKIAFDDKGDIKNGAVTVYQVKNGKWEVVSTVGGEA
K

Sequences:

>Translated_401_residues
MNKYARYSLIATAVIAMNACSKQEQPASGGQAASAPSQAAAGGGETVVKIGQVSPMSGPISHLGKDNEYGAKLAIEDLNA
KGVEIGGKKVKFELVSEDDQGDPKIGTQVAQRLVDAGVVGVVGHLNSGTTIPASKIYSDAGLPQISPSATNPDYTKQGFK
TTFRVIANDVQQGKALGEFAAGTLNAKKIAIIDDRTAYGQGLADQFEGAVKAKGAQVVKREFTNNTATDFNAILTSIKAT
NPDLVFYGGMDAQAGPLAKQMQRLGIKAKLMGGDGWQTPEFVKLAGDASEGQYASSCGISRDKMPGFKAFDEKFKKEFNT
DVQIYAPFEYDAVMVLVDAMKRAKSYDPKAYLPEVGKTDYQGVTGKIAFDDKGDIKNGAVTVYQVKNGKWEVVSTVGGEA
K
>Mature_401_residues
MNKYARYSLIATAVIAMNACSKQEQPASGGQAASAPSQAAAGGGETVVKIGQVSPMSGPISHLGKDNEYGAKLAIEDLNA
KGVEIGGKKVKFELVSEDDQGDPKIGTQVAQRLVDAGVVGVVGHLNSGTTIPASKIYSDAGLPQISPSATNPDYTKQGFK
TTFRVIANDVQQGKALGEFAAGTLNAKKIAIIDDRTAYGQGLADQFEGAVKAKGAQVVKREFTNNTATDFNAILTSIKAT
NPDLVFYGGMDAQAGPLAKQMQRLGIKAKLMGGDGWQTPEFVKLAGDASEGQYASSCGISRDKMPGFKAFDEKFKKEFNT
DVQIYAPFEYDAVMVLVDAMKRAKSYDPKAYLPEVGKTDYQGVTGKIAFDDKGDIKNGAVTVYQVKNGKWEVVSTVGGEA
K

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=337, Percent_Identity=34.7181008902077, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI48994941, Length=329, Percent_Identity=31.0030395136778, Blast_Score=161, Evalue=9e-41,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 42366; Mature: 42366

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKYARYSLIATAVIAMNACSKQEQPASGGQAASAPSQAAAGGGETVVKIGQVSPMSGPI
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCEEEEECCCCCCCCCH
SHLGKDNEYGAKLAIEDLNAKGVEIGGKKVKFELVSEDDQGDPKIGTQVAQRLVDAGVVG
HHCCCCCCCCCEEEEEECCCCCEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEE
VVGHLNSGTTIPASKIYSDAGLPQISPSATNPDYTKQGFKTTFRVIANDVQQGKALGEFA
EEEECCCCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGTLNAKKIAIIDDRTAYGQGLADQFEGAVKAKGAQVVKREFTNNTATDFNAILTSIKAT
HCCCCCCEEEEEECCCHHCCCCHHHHCCHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCC
NPDLVFYGGMDAQAGPLAKQMQRLGIKAKLMGGDGWQTPEFVKLAGDASEGQYASSCGIS
CCCEEEECCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHEEEECCCCCCCCHHHCCCC
RDKMPGFKAFDEKFKKEFNTDVQIYAPFEYDAVMVLVDAMKRAKSYDPKAYLPEVGKTDY
CCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
QGVTGKIAFDDKGDIKNGAVTVYQVKNGKWEVVSTVGGEAK
CCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCCC
>Mature Secondary Structure
MNKYARYSLIATAVIAMNACSKQEQPASGGQAASAPSQAAAGGGETVVKIGQVSPMSGPI
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCEEEEECCCCCCCCCH
SHLGKDNEYGAKLAIEDLNAKGVEIGGKKVKFELVSEDDQGDPKIGTQVAQRLVDAGVVG
HHCCCCCCCCCEEEEEECCCCCEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEE
VVGHLNSGTTIPASKIYSDAGLPQISPSATNPDYTKQGFKTTFRVIANDVQQGKALGEFA
EEEECCCCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGTLNAKKIAIIDDRTAYGQGLADQFEGAVKAKGAQVVKREFTNNTATDFNAILTSIKAT
HCCCCCCEEEEEECCCHHCCCCHHHHCCHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCC
NPDLVFYGGMDAQAGPLAKQMQRLGIKAKLMGGDGWQTPEFVKLAGDASEGQYASSCGIS
CCCEEEECCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHEEEECCCCCCCCHHHCCCC
RDKMPGFKAFDEKFKKEFNTDVQIYAPFEYDAVMVLVDAMKRAKSYDPKAYLPEVGKTDY
CCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
QGVTGKIAFDDKGDIKNGAVTVYQVKNGKWEVVSTVGGEAK
CCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]