Click here to switch to the map view.

The map label for this gene is ribH

Identifier: 34497845

GI number: 34497845

Start: 2572153

End: 2572620

Strand: Direct

Name: ribH

Synonym: CV_2390

Alternate gene names: 34497845

Gene position: 2572153-2572620 (Clockwise)

Preceding gene: 34497844

Following gene: 34497846

Centisome position: 54.14

GC content: 63.68

Gene sequence:

>468_bases
ATGCTTGACGCCATCCAGTGTATTGAACCCAATTTTTCCGGCAAAGGGCTGAAAATCGGCATCGCCATGGCGCGTTTCAA
CACGCCGGTTTGCCACGGCCTGCGCGACGCTTGTCTCGCCGAGCTGGAAAAACTGGGCGTCGCCGCCGGCGACATCACCC
TGGCCACAGTCCCGGGCGCGCTGGAGGTTCCTCTGGTGCTGCAAACCATGGCCAAGAGCGGTCGTTTCGACGCGCTGGTG
GCGCTGGGCGCAGTGATCCGCGGCGAAACCTATCATTTCGAGCTGGTGTCGAACGAATCCGGCGCCGGCGTCACCCGCGT
CGGCCTGGATTTCGATATTCCGGTCGCCAACGCCATCCTGACCACCGAGAACGACGAGCAGGCGGAAGTCCGCATGCTGG
AAAAGGGCGGCGATGCCGCCCGCGTGGCGGTGGAGATGGCCAATCTGCACAAGACGCTGCGCGGCTGA

Upstream 100 bases:

>100_bases
CTTCCACGTGGACCGGGACGGCGGTCCGGGCGTGGCCTAATCGCGGCGATTGCGTTACAATTGCCGGCTTTCCTTAGTGT
TTTCGCCGCGAGGACCCAAC

Downstream 100 bases:

>100_bases
GCCGTTCGCCGCCAGTCGTCCAACGGGAAGCGGGGCGGTCGCGCCGCTTCCCTTGCTCTTTCAAGGAATCGCCACCATGA
AAACCGCCCGTCGCCGCGCC

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MLDAIQCIEPNFSGKGLKIGIAMARFNTPVCHGLRDACLAELEKLGVAAGDITLATVPGALEVPLVLQTMAKSGRFDALV
ALGAVIRGETYHFELVSNESGAGVTRVGLDFDIPVANAILTTENDEQAEVRMLEKGGDAARVAVEMANLHKTLRG

Sequences:

>Translated_155_residues
MLDAIQCIEPNFSGKGLKIGIAMARFNTPVCHGLRDACLAELEKLGVAAGDITLATVPGALEVPLVLQTMAKSGRFDALV
ALGAVIRGETYHFELVSNESGAGVTRVGLDFDIPVANAILTTENDEQAEVRMLEKGGDAARVAVEMANLHKTLRG
>Mature_155_residues
MLDAIQCIEPNFSGKGLKIGIAMARFNTPVCHGLRDACLAELEKLGVAAGDITLATVPGALEVPLVLQTMAKSGRFDALV
ALGAVIRGETYHFELVSNESGAGVTRVGLDFDIPVANAILTTENDEQAEVRMLEKGGDAARVAVEMANLHKTLRG

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=155, Percent_Identity=39.3548387096774, Blast_Score=106, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6324429, Length=149, Percent_Identity=30.8724832214765, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_CHRVO (Q7NVF3)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_902060.1
- ProteinModelPortal:   Q7NVF3
- SMR:   Q7NVF3
- GeneID:   2548929
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_2390
- NMPDR:   fig|243365.1.peg.2390
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- PhylomeDB:   Q7NVF3
- ProtClustDB:   PRK00061
- BioCyc:   CVIO243365:CV_2390-MONOMER
- BRENDA:   2.5.1.9
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16331; Mature: 16331

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDAIQCIEPNFSGKGLKIGIAMARFNTPVCHGLRDACLAELEKLGVAAGDITLATVPGA
CCCCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
LEVPLVLQTMAKSGRFDALVALGAVIRGETYHFELVSNESGAGVTRVGLDFDIPVANAIL
CCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEECCEECCCCCCEEE
TTENDEQAEVRMLEKGGDAARVAVEMANLHKTLRG
ECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLDAIQCIEPNFSGKGLKIGIAMARFNTPVCHGLRDACLAELEKLGVAAGDITLATVPGA
CCCCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
LEVPLVLQTMAKSGRFDALVALGAVIRGETYHFELVSNESGAGVTRVGLDFDIPVANAIL
CCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEECCEECCCCCCEEE
TTENDEQAEVRMLEKGGDAARVAVEMANLHKTLRG
ECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782