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The map label for this gene is cysD

Identifier: 34497761

GI number: 34497761

Start: 2496393

End: 2497319

Strand: Direct

Name: cysD

Synonym: CV_2306

Alternate gene names: 34497761

Gene position: 2496393-2497319 (Clockwise)

Preceding gene: 34497756

Following gene: 34497762

Centisome position: 52.54

GC content: 61.38

Gene sequence:

>927_bases
ATGGATATCGATCAAGCAAGGCTGACCCATCTCCAGCAATTGGAGGCGGAGTCCATCCACATCATCCGCGAGGTGGCGGC
GGAATTCGAGAACCCGGTGATGCTGTACTCCATCGGCAAGGATTCCGCGGTGATGCTGCACCTGGCGAAGAAGGCTTTCT
ACCCGGGCAAGCCGCCGTTCCCGCTGATGCACGTCGATACCACCTGGAAATTCCGCGACATGATCGCGCTGCGCGACAAG
CAGGCGAAGGAAAACGGCTGGGATCTGATCGTCCACGTCAATCAGGACGGCGTGGAGGCCGGCATCAATCCGTTCACCGC
CGGCAGCGCCAAGCATACCGACGTGATGAAGACCGAGGGCCTGAAGCAGGCGCTGAACCGGCACAAGTTCGACGCGGCTT
TCGGCGGCGCGCGGCGCGACGAGGAGAAAAGCCGCGCCAAGGAGCGCGTCTACTCCTTCCGCGACAAGAACCACCGCTGG
GATCCGAAAAACCAGCGGCCGGAGCTGTGGAACATCTACAACGGCAGGATCGACAAGGGCGAGAGCATTCGCGTGTTCCC
GCTGTCCAACTGGACCGAGCTGGACATCTGGCAATACATCTACCTGGAGAACATCGAGATCGTGCCCTTGTATTTCGCCG
CCGAACGCCCGGTGGTGGAGCGCGACGGCATGCTGATCATGATCGACGACGAGCGCATCCTCGAATACCTGACCCCGGAG
GAGAAGGCGAGCATCCAGACCCGCAAGGTGCGTTTCCGCACGCTGGGCTGTTACCCGCTGACCGGCGCGGTGGAGTCGGA
GGCCGCCACGCTGCCGGAGATCATCCAGGAAATGCTGCTGACGAGGACTTCCGAGCGGCAGGGCCGGCTGATCGACCATG
ACAGCGCCGGCTCGATGGAAAAGAAGAAAATGGAAGGGTATTTCTGA

Upstream 100 bases:

>100_bases
TTTTAAGATTTGCTAAGTTTATATGGATATATCCAATCGGTTTTGAATACGCGCGAACATTTCCGGTAGATTGGCTGCAA
TCATAGAACAGGCTGCAAGC

Downstream 100 bases:

>100_bases
TGTCGCACCAATCCGAACTGATCGCCGGGGATATCCTGGAATACCTGAGCCAGCATGAGAACAAGGACATGCTGCGCTTC
ATCACCTGCGGCAGCGTGGA

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MDIDQARLTHLQQLEAESIHIIREVAAEFENPVMLYSIGKDSAVMLHLAKKAFYPGKPPFPLMHVDTTWKFRDMIALRDK
QAKENGWDLIVHVNQDGVEAGINPFTAGSAKHTDVMKTEGLKQALNRHKFDAAFGGARRDEEKSRAKERVYSFRDKNHRW
DPKNQRPELWNIYNGRIDKGESIRVFPLSNWTELDIWQYIYLENIEIVPLYFAAERPVVERDGMLIMIDDERILEYLTPE
EKASIQTRKVRFRTLGCYPLTGAVESEAATLPEIIQEMLLTRTSERQGRLIDHDSAGSMEKKKMEGYF

Sequences:

>Translated_308_residues
MDIDQARLTHLQQLEAESIHIIREVAAEFENPVMLYSIGKDSAVMLHLAKKAFYPGKPPFPLMHVDTTWKFRDMIALRDK
QAKENGWDLIVHVNQDGVEAGINPFTAGSAKHTDVMKTEGLKQALNRHKFDAAFGGARRDEEKSRAKERVYSFRDKNHRW
DPKNQRPELWNIYNGRIDKGESIRVFPLSNWTELDIWQYIYLENIEIVPLYFAAERPVVERDGMLIMIDDERILEYLTPE
EKASIQTRKVRFRTLGCYPLTGAVESEAATLPEIIQEMLLTRTSERQGRLIDHDSAGSMEKKKMEGYF
>Mature_308_residues
MDIDQARLTHLQQLEAESIHIIREVAAEFENPVMLYSIGKDSAVMLHLAKKAFYPGKPPFPLMHVDTTWKFRDMIALRDK
QAKENGWDLIVHVNQDGVEAGINPFTAGSAKHTDVMKTEGLKQALNRHKFDAAFGGARRDEEKSRAKERVYSFRDKNHRW
DPKNQRPELWNIYNGRIDKGESIRVFPLSNWTELDIWQYIYLENIEIVPLYFAAERPVVERDGMLIMIDDERILEYLTPE
EKASIQTRKVRFRTLGCYPLTGAVESEAATLPEIIQEMLLTRTSERQGRLIDHDSAGSMEKKKMEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily

Homologues:

Organism=Escherichia coli, GI1789109, Length=306, Percent_Identity=77.124183006536, Blast_Score=486, Evalue=1e-139,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSD_CHRVO (Q7NVN6)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901976.1
- HSSP:   Q87WW0
- ProteinModelPortal:   Q7NVN6
- SMR:   Q7NVN6
- GeneID:   2549874
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_2306
- NMPDR:   fig|243365.1.peg.2306
- HOGENOM:   HBG758022
- OMA:   HYGFDAA
- PhylomeDB:   Q7NVN6
- ProtClustDB:   PRK05253
- BioCyc:   CVIO243365:CV_2306-MONOMER
- BRENDA:   2.7.7.4
- HAMAP:   MF_00064
- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF002936
- TIGRFAMs:   TIGR02039

Pfam domain/function: PF01507 PAPS_reduct

EC number: =2.7.7.4

Molecular weight: Translated: 35697; Mature: 35697

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIDQARLTHLQQLEAESIHIIREVAAEFENPVMLYSIGKDSAVMLHLAKKAFYPGKPPF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEHHEEHHHHCCCCCCCC
PLMHVDTTWKFRDMIALRDKQAKENGWDLIVHVNQDGVEAGINPFTAGSAKHTDVMKTEG
CEEEECCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHCCCCCCCCCCCCCHHHHHHH
LKQALNRHKFDAAFGGARRDEEKSRAKERVYSFRDKNHRWDPKNQRPELWNIYNGRIDKG
HHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCC
ESIRVFPLSNWTELDIWQYIYLENIEIVPLYFAAERPVVERDGMLIMIDDERILEYLTPE
CCEEEEECCCCCCHHHEEEEEECCEEEEEEEEECCCCEEECCCEEEEECCHHHHHHCCCC
EKASIQTRKVRFRTLGCYPLTGAVESEAATLPEIIQEMLLTRTSERQGRLIDHDSAGSME
HHHCHHHEEEEEEEECCEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCH
KKKMEGYF
HHHHCCCC
>Mature Secondary Structure
MDIDQARLTHLQQLEAESIHIIREVAAEFENPVMLYSIGKDSAVMLHLAKKAFYPGKPPF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEHHEEHHHHCCCCCCCC
PLMHVDTTWKFRDMIALRDKQAKENGWDLIVHVNQDGVEAGINPFTAGSAKHTDVMKTEG
CEEEECCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHCCCCCCCCCCCCCHHHHHHH
LKQALNRHKFDAAFGGARRDEEKSRAKERVYSFRDKNHRWDPKNQRPELWNIYNGRIDKG
HHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCC
ESIRVFPLSNWTELDIWQYIYLENIEIVPLYFAAERPVVERDGMLIMIDDERILEYLTPE
CCEEEEECCCCCCHHHEEEEEECCEEEEEEEEECCCCEEECCCEEEEECCHHHHHHCCCC
EKASIQTRKVRFRTLGCYPLTGAVESEAATLPEIIQEMLLTRTSERQGRLIDHDSAGSME
HHHCHHHEEEEEEEECCEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCH
KKKMEGYF
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782