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The map label for this gene is gph [C]

Identifier: 34497635

GI number: 34497635

Start: 2353120

End: 2353788

Strand: Reverse

Name: gph [C]

Synonym: CV_2180

Alternate gene names: 34497635

Gene position: 2353788-2353120 (Counterclockwise)

Preceding gene: 34497636

Following gene: 34497634

Centisome position: 49.54

GC content: 67.26

Gene sequence:

>669_bases
ATGAATCTCAAGCACATCAAAGCCGTCGCCTTCGACCTGGACGGCACTCTGGTCGACTCCATTCCCGATCTGGCCAATGC
CGCCAACGCGATGCGCGAGCATCTGGGCCTGCCGCCGCTGGATCCCGAACGGATCAAGAGCCACGTCGGCGACGGCATCG
CCAGCCTGGTCCACCGCGCCATCACCGACGAGCGCCACGCCGAGGCCGACGGCCCGCTGTGGGAGCGCGGCTATCGCTTC
TTCGTCCAGCGCTACCGTGAACATCTGGCCGACCACACCACCGTCTACCCCGGCGTGCGCGACGGCCTGGGCCTGTTGCG
CGCGCTTCAGCTGCCGCTGGTCATGATCACCAACAAATCCGAACGCCTGGCCGTGCCGCTGGCCGAGCAGCTGGGCCTGC
GCGACCATTTCAGCCTGATCGTCGGCGGCGACACGCTGCCGGAGAAGAAGCCGTCGGCTCTGCCGCTGCTGCATTGCTGC
CAGGTGCTGGGCATCCAGCCGCAGGAACTGGCGATGGTGGGCGACTCCGCCAATGACGTGGCCGCCGCCCGCGCCGCCGG
CTGCGCGGCCATCGCCGTCGGCTACGGCTACGCCGACGCGTCCACGCTGGGCGCGGACCTGACGGTAAACAGTATTGCCG
AGCTCTACGATTTGATGAAGAATGGCTAA

Upstream 100 bases:

>100_bases
CCGGCTTTCGGCCGGCAGCCCGTCGTCGACATCCGCGAACTCTGAACACCCTCATCCCCATTCCGCCGCGGCCCGCGCGC
CGCGGCAACAAAGCAGACAC

Downstream 100 bases:

>100_bases
AGCCCTGCAACCCTGAAGGAACTCGAACGCCCATGGCCTTTCTTCCCTGCCCCGCCAGCTGGCGATGGCGTCGCCCGGTC
CCCGCCCCGCACGACTCCGC

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MNLKHIKAVAFDLDGTLVDSIPDLANAANAMREHLGLPPLDPERIKSHVGDGIASLVHRAITDERHAEADGPLWERGYRF
FVQRYREHLADHTTVYPGVRDGLGLLRALQLPLVMITNKSERLAVPLAEQLGLRDHFSLIVGGDTLPEKKPSALPLLHCC
QVLGIQPQELAMVGDSANDVAAARAAGCAAIAVGYGYADASTLGADLTVNSIAELYDLMKNG

Sequences:

>Translated_222_residues
MNLKHIKAVAFDLDGTLVDSIPDLANAANAMREHLGLPPLDPERIKSHVGDGIASLVHRAITDERHAEADGPLWERGYRF
FVQRYREHLADHTTVYPGVRDGLGLLRALQLPLVMITNKSERLAVPLAEQLGLRDHFSLIVGGDTLPEKKPSALPLLHCC
QVLGIQPQELAMVGDSANDVAAARAAGCAAIAVGYGYADASTLGADLTVNSIAELYDLMKNG
>Mature_222_residues
MNLKHIKAVAFDLDGTLVDSIPDLANAANAMREHLGLPPLDPERIKSHVGDGIASLVHRAITDERHAEADGPLWERGYRF
FVQRYREHLADHTTVYPGVRDGLGLLRALQLPLVMITNKSERLAVPLAEQLGLRDHFSLIVGGDTLPEKKPSALPLLHCC
QVLGIQPQELAMVGDSANDVAAARAAGCAAIAVGYGYADASTLGADLTVNSIAELYDLMKNG

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family

Homologues:

Organism=Escherichia coli, GI1789787, Length=212, Percent_Identity=40.5660377358491, Blast_Score=141, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GPH_CHRVO (Q7NW10)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901850.1
- ProteinModelPortal:   Q7NW10
- SMR:   Q7NW10
- GeneID:   2549384
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_2180
- NMPDR:   fig|243365.1.peg.2180
- HOGENOM:   HBG742904
- OMA:   YNYGESI
- PhylomeDB:   Q7NW10
- ProtClustDB:   PRK13222
- BioCyc:   CVIO243365:CV_2180-MONOMER
- BRENDA:   3.1.3.18
- HAMAP:   MF_00495
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198
- Gene3D:   G3DSA:3.40.50.1000
- Gene3D:   G3DSA:1.10.150.240
- TIGRFAMs:   TIGR01549
- TIGRFAMs:   TIGR01509
- TIGRFAMs:   TIGR01449

Pfam domain/function: PF00702 Hydrolase; SSF56784 SSF56784

EC number: =3.1.3.18

Molecular weight: Translated: 23874; Mature: 23874

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: ACT_SITE 12-12

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKHIKAVAFDLDGTLVDSIPDLANAANAMREHLGLPPLDPERIKSHVGDGIASLVHRA
CCCHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHHH
ITDERHAEADGPLWERGYRFFVQRYREHLADHTTVYPGVRDGLGLLRALQLPLVMITNKS
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEECCCHHHHHHHHHHHHCCEEEEECCC
ERLAVPLAEQLGLRDHFSLIVGGDTLPEKKPSALPLLHCCQVLGIQPQELAMVGDSANDV
CCEECCHHHHCCCCHHCEEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHHHEECCCCHHH
AAARAAGCAAIAVGYGYADASTLGADLTVNSIAELYDLMKNG
HHHHHHCHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLKHIKAVAFDLDGTLVDSIPDLANAANAMREHLGLPPLDPERIKSHVGDGIASLVHRA
CCCHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHHH
ITDERHAEADGPLWERGYRFFVQRYREHLADHTTVYPGVRDGLGLLRALQLPLVMITNKS
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEECCCHHHHHHHHHHHHCCEEEEECCC
ERLAVPLAEQLGLRDHFSLIVGGDTLPEKKPSALPLLHCCQVLGIQPQELAMVGDSANDV
CCEECCHHHHCCCCHHCEEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHHHEECCCCHHH
AAARAAGCAAIAVGYGYADASTLGADLTVNSIAELYDLMKNG
HHHHHHCHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782