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The map label for this gene is trpE [H]

Identifier: 34497634

GI number: 34497634

Start: 2351524

End: 2352990

Strand: Reverse

Name: trpE [H]

Synonym: CV_2179

Alternate gene names: 34497634

Gene position: 2352990-2351524 (Counterclockwise)

Preceding gene: 34497635

Following gene: 34497631

Centisome position: 49.53

GC content: 67.01

Gene sequence:

>1467_bases
ATGCAAGCGCAAGAGTTTGACCAACTGGCCGCCGCCGGCTACAACCGCATCCCCGTCACCCAGGAACTGCTGGCCGATCT
GGACAGCCCGCTGTCCATCTATCTGAAGCTGGCCAACCAGCCGTACAGCTACCTGCTGGAATCGGTGGTGGGCGGCGAGC
GCCACGGCCGCTACTCCATCATCGGCCTGCCGGCCGACACCCGGCTGCGCGTCAACGGCCAGCGGGTGCAGGTGGAATGC
GGCGACAAGGTGATAGAACGCCACGACGGCGACCCGCTGGCCTTCATCGAGGCGTTCCAGCACCGTTTCCGTATCCCCGA
GCTGCCCGGCCTGCCGCGCTACTCCGGCGGCCTGGTCGGCTATTTCGGCTACGACACCATACGCTACATTGAACGCCGCC
TCGCCGACGTTCAGAAACCCGACCCGGTGGGCACACCGGACATCCTGCTGATGCTGTCGGAAGAGATCGCCGTGGTCGAC
AACCTGTCCGGCAAGCTCTACCTGGTGGTCTACGCCGACCCCGAGGTGCCCAACGCGCTGCAGAAAGCGAAAGCGCGGCT
GGCCCAGCTTCGCGCCAAGCTGCGCGAGCCGCTGGCCATCCCGCTGTCGCTGCCCCAGCCGCACGAGGGCGAGGCGGTGT
CGGAATACGGCGAGGAGGCGTTCAAGCAGGCGGTGGCGTCCTGTCAGCGCTACATCCACGACGGCGACATCATGCAGGTG
GTGCTGGCCCAGCGCATGCAGCAGCCTTACCACGGCTCGCCGATCGCGCTGTACCGCGCGCTGCGCAACCTCAACCCGTC
GCCGTACATGTTCTTCTACCACTTCGACGACTTCCACGTGGTGGGCGCTTCGCCGGAAATCCTGGTGCGGCGCGAAGGCG
AAACCGTGACCGTGCGTCCGATCGCCGGCACCCGCCCGCGCGGCCACACCCGCGAGCAGGATGAGGCGCTGGCGGCCGAG
CTGCTGGCCGACCCCAAGGAAATCGCCGAACACGTGATGCTGATGGACCTGGGCCGCAACGACGTCGGCCGCGTCGCCGA
CATCGGCACGGTGAAGATCACCGACAACATGGCGATCGAGCGCTACTCGCACGTGATGCACATCGTGTCCAACGTGGAAG
GCCAGGTGGACCGCAAGATCAGCAATATCGACGTGATCAAGGCCACCTTCCCCGCCGGCACGCTGTCCGGCGCGCCCAAG
GTGCGGGCGATGCAGATCATCGACGAGTTCGAGCCGACCAAGCGCGGCGTCTACGGCGGCGCGGTGGGTTACCTCGGCTT
CAACGGCGACATGGACCTCGCCATCGCCATCCGCACCGCGGTGCTGAAGAACGGCATGCTCTACGTGCAGTCCGGCGCCG
GCGTCGTCGCCGACTCGGTGCCGCAATCGGAATGGCAGGAAACGCAGAACAAGGCGCGCGCGGTGCTGCGCGCCGCGCAG
ATGGTGCGGGACGGCCTGGACGCCTGA

Upstream 100 bases:

>100_bases
CCCATGGCCTTTCTTCCCTGCCCCGCCAGCTGGCGATGGCGTCGCCCGGTCCCCGCCCCGCACGACTCCGCCCACACTGA
CAAGACACAGGAAGAACCAC

Downstream 100 bases:

>100_bases
GCCTCCGTCACATGAAACGCCGGCCGCTGCCGGCGTTTCATGCCGCCAGATCCCTGCCCACCTGCCAGGACAAAGGCAGG
GTCAATAACAGGAAGGCGGC

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MQAQEFDQLAAAGYNRIPVTQELLADLDSPLSIYLKLANQPYSYLLESVVGGERHGRYSIIGLPADTRLRVNGQRVQVEC
GDKVIERHDGDPLAFIEAFQHRFRIPELPGLPRYSGGLVGYFGYDTIRYIERRLADVQKPDPVGTPDILLMLSEEIAVVD
NLSGKLYLVVYADPEVPNALQKAKARLAQLRAKLREPLAIPLSLPQPHEGEAVSEYGEEAFKQAVASCQRYIHDGDIMQV
VLAQRMQQPYHGSPIALYRALRNLNPSPYMFFYHFDDFHVVGASPEILVRREGETVTVRPIAGTRPRGHTREQDEALAAE
LLADPKEIAEHVMLMDLGRNDVGRVADIGTVKITDNMAIERYSHVMHIVSNVEGQVDRKISNIDVIKATFPAGTLSGAPK
VRAMQIIDEFEPTKRGVYGGAVGYLGFNGDMDLAIAIRTAVLKNGMLYVQSGAGVVADSVPQSEWQETQNKARAVLRAAQ
MVRDGLDA

Sequences:

>Translated_488_residues
MQAQEFDQLAAAGYNRIPVTQELLADLDSPLSIYLKLANQPYSYLLESVVGGERHGRYSIIGLPADTRLRVNGQRVQVEC
GDKVIERHDGDPLAFIEAFQHRFRIPELPGLPRYSGGLVGYFGYDTIRYIERRLADVQKPDPVGTPDILLMLSEEIAVVD
NLSGKLYLVVYADPEVPNALQKAKARLAQLRAKLREPLAIPLSLPQPHEGEAVSEYGEEAFKQAVASCQRYIHDGDIMQV
VLAQRMQQPYHGSPIALYRALRNLNPSPYMFFYHFDDFHVVGASPEILVRREGETVTVRPIAGTRPRGHTREQDEALAAE
LLADPKEIAEHVMLMDLGRNDVGRVADIGTVKITDNMAIERYSHVMHIVSNVEGQVDRKISNIDVIKATFPAGTLSGAPK
VRAMQIIDEFEPTKRGVYGGAVGYLGFNGDMDLAIAIRTAVLKNGMLYVQSGAGVVADSVPQSEWQETQNKARAVLRAAQ
MVRDGLDA
>Mature_488_residues
MQAQEFDQLAAAGYNRIPVTQELLADLDSPLSIYLKLANQPYSYLLESVVGGERHGRYSIIGLPADTRLRVNGQRVQVEC
GDKVIERHDGDPLAFIEAFQHRFRIPELPGLPRYSGGLVGYFGYDTIRYIERRLADVQKPDPVGTPDILLMLSEEIAVVD
NLSGKLYLVVYADPEVPNALQKAKARLAQLRAKLREPLAIPLSLPQPHEGEAVSEYGEEAFKQAVASCQRYIHDGDIMQV
VLAQRMQQPYHGSPIALYRALRNLNPSPYMFFYHFDDFHVVGASPEILVRREGETVTVRPIAGTRPRGHTREQDEALAAE
LLADPKEIAEHVMLMDLGRNDVGRVADIGTVKITDNMAIERYSHVMHIVSNVEGQVDRKISNIDVIKATFPAGTLSGAPK
VRAMQIIDEFEPTKRGVYGGAVGYLGFNGDMDLAIAIRTAVLKNGMLYVQSGAGVVADSVPQSEWQETQNKARAVLRAAQ
MVRDGLDA

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=398, Percent_Identity=41.4572864321608, Blast_Score=261, Evalue=9e-71,
Organism=Escherichia coli, GI1788114, Length=462, Percent_Identity=32.2510822510823, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI87082077, Length=267, Percent_Identity=27.3408239700375, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1786809, Length=213, Percent_Identity=30.0469483568075, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320935, Length=474, Percent_Identity=38.8185654008439, Blast_Score=297, Evalue=3e-81,
Organism=Saccharomyces cerevisiae, GI6324361, Length=495, Percent_Identity=25.6565656565657, Blast_Score=147, Evalue=4e-36,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 54023; Mature: 54023

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAQEFDQLAAAGYNRIPVTQELLADLDSPLSIYLKLANQPYSYLLESVVGGERHGRYSI
CCHHHHHHHHHCCCCCCCCHHHHHHCCCCCCEEHHEECCCCHHHHHHHHHCCCCCCCEEE
IGLPADTRLRVNGQRVQVECGDKVIERHDGDPLAFIEAFQHRFRIPELPGLPRYSGGLVG
EECCCCCEEEECCCEEEEECCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEE
YFGYDTIRYIERRLADVQKPDPVGTPDILLMLSEEIAVVDNLSGKLYLVVYADPEVPNAL
EEHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHEEECCCCCEEEEEEECCCCCHHH
QKAKARLAQLRAKLREPLAIPLSLPQPHEGEAVSEYGEEAFKQAVASCQRYIHDGDIMQV
HHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VLAQRMQQPYHGSPIALYRALRNLNPSPYMFFYHFDDFHVVGASPEILVRREGETVTVRP
HHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCEEEECCCCCEEEEECCCEEEEEE
IAGTRPRGHTREQDEALAAELLADPKEIAEHVMLMDLGRNDVGRVADIGTVKITDNMAIE
CCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEECCCEEEEECCHHHH
RYSHVMHIVSNVEGQVDRKISNIDVIKATFPAGTLSGAPKVRAMQIIDEFEPTKRGVYGG
HHHHHHHHHHHCCCHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCHHHCCCCCC
AVGYLGFNGDMDLAIAIRTAVLKNGMLYVQSGAGVVADSVPQSEWQETQNKARAVLRAAQ
EEEEEEECCCCEEHHHHHHHHHHCCEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHH
MVRDGLDA
HHHHCCCC
>Mature Secondary Structure
MQAQEFDQLAAAGYNRIPVTQELLADLDSPLSIYLKLANQPYSYLLESVVGGERHGRYSI
CCHHHHHHHHHCCCCCCCCHHHHHHCCCCCCEEHHEECCCCHHHHHHHHHCCCCCCCEEE
IGLPADTRLRVNGQRVQVECGDKVIERHDGDPLAFIEAFQHRFRIPELPGLPRYSGGLVG
EECCCCCEEEECCCEEEEECCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEE
YFGYDTIRYIERRLADVQKPDPVGTPDILLMLSEEIAVVDNLSGKLYLVVYADPEVPNAL
EEHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHEEECCCCCEEEEEEECCCCCHHH
QKAKARLAQLRAKLREPLAIPLSLPQPHEGEAVSEYGEEAFKQAVASCQRYIHDGDIMQV
HHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VLAQRMQQPYHGSPIALYRALRNLNPSPYMFFYHFDDFHVVGASPEILVRREGETVTVRP
HHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCEEEECCCCCEEEEECCCEEEEEE
IAGTRPRGHTREQDEALAAELLADPKEIAEHVMLMDLGRNDVGRVADIGTVKITDNMAIE
CCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEECCCEEEEECCHHHH
RYSHVMHIVSNVEGQVDRKISNIDVIKATFPAGTLSGAPKVRAMQIIDEFEPTKRGVYGG
HHHHHHHHHHHCCCHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCHHHCCCCCC
AVGYLGFNGDMDLAIAIRTAVLKNGMLYVQSGAGVVADSVPQSEWQETQNKARAVLRAAQ
EEEEEEECCCCEEHHHHHHHHHHCCEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHH
MVRDGLDA
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10417653 [H]