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The map label for this gene is dmlR [H]

Identifier: 34497625

GI number: 34497625

Start: 2343166

End: 2344086

Strand: Direct

Name: dmlR [H]

Synonym: CV_2170

Alternate gene names: 34497625

Gene position: 2343166-2344086 (Clockwise)

Preceding gene: 34497621

Following gene: 34497632

Centisome position: 49.32

GC content: 62.54

Gene sequence:

>921_bases
ATGAGCTCGGTATCCGATGTGGCTTTTTTCATGGAAGTGGTCAAGGCGGGCACGCTGTCCGGCGCGGCGCAGGAGCTTGG
CGTATCGACAGCCGCCGTCAGCCGGCGCCTGGCCAATCTCGAGGTGCGGCTCGGCATCCGGCTGTTGAACCGCACGACGA
GACGGGCGGCCGTTACGTATGAAGGCGAGCTGTATCTGGCGGAGGGGAAAAAGATTCTTGGGGAGCTGGAGGAATTGGAG
CAAAGGCTGGCCAGCGCGCACTCCGTCCCGAAGGGGCTGTTGCGGGTCAATGCGACCTTCGGCTTCGGCCGCCAGTTCAT
CGCCCCGGCGATAGCGGATTTTGTCGAGCTTCATCCCGATGTCGACGTTCAGCTGTCCCTGTCCGACCGCCCGGTCAATC
TGATCGAGGACGGATTCGATGTCTGCATCCGCTTCGGCGACCTGCCCGATGCGCGTCTCACATCCAGGAAGATCGCCTGC
AATCGCCGCCTGTTATGCGCGTCGCCGGGCTATCTGGAGCGTTTCGGCATCCCGGCGTCTCCGATGGATCTGCAGCGCCA
CCGTTGCATCGTGATCCGGGAAAGCGATGAAACCTATGGCGCGTGGCATCTTTACTCGGGAGCCGCGCAAGCCAATGTGA
AGGTTCGAGGGACGGTGTCGACCAACGATGGAGAAGTGGCTGTCGACTGGGCGTTGCGGGGCTTGGGGATATTGCTGCGC
TCGGAGTGGAATGTCGCGCCCTATCTGCGCTCGGGCCGATTGCGCGAAGTGCTCGGCGACTGGCGGTTTCCGCCCGCCGA
CATTCACGCGGTCTATCCGATGAAGAGCAACCTGTCGGCGAAAGTGCGGGCGTTCGTCAGCCATCTGGACAAGCATTTTG
AGCCATACCGGCCCAGAGCGGAGGGCAAGAGTCGCTGGTAG

Upstream 100 bases:

>100_bases
AGCCTGAAAGCGCGGAGGCTTCCTTGGGAGGCAAATTATTGGATTGCAGATTTTTGTACAATAATGGAAATATCAAACTG
TTTTTAACTTGAATGCAAAA

Downstream 100 bases:

>100_bases
CGCTCGGCAAAGCCGCATTGACCTGGGTGATTTGGCTCTTATCGATGAGGCCGGAAAGATAGGAATAGATGATAAAAATA
GCCGCCAACGTTGTTGGCGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSSVSDVAFFMEVVKAGTLSGAAQELGVSTAAVSRRLANLEVRLGIRLLNRTTRRAAVTYEGELYLAEGKKILGELEELE
QRLASAHSVPKGLLRVNATFGFGRQFIAPAIADFVELHPDVDVQLSLSDRPVNLIEDGFDVCIRFGDLPDARLTSRKIAC
NRRLLCASPGYLERFGIPASPMDLQRHRCIVIRESDETYGAWHLYSGAAQANVKVRGTVSTNDGEVAVDWALRGLGILLR
SEWNVAPYLRSGRLREVLGDWRFPPADIHAVYPMKSNLSAKVRAFVSHLDKHFEPYRPRAEGKSRW

Sequences:

>Translated_306_residues
MSSVSDVAFFMEVVKAGTLSGAAQELGVSTAAVSRRLANLEVRLGIRLLNRTTRRAAVTYEGELYLAEGKKILGELEELE
QRLASAHSVPKGLLRVNATFGFGRQFIAPAIADFVELHPDVDVQLSLSDRPVNLIEDGFDVCIRFGDLPDARLTSRKIAC
NRRLLCASPGYLERFGIPASPMDLQRHRCIVIRESDETYGAWHLYSGAAQANVKVRGTVSTNDGEVAVDWALRGLGILLR
SEWNVAPYLRSGRLREVLGDWRFPPADIHAVYPMKSNLSAKVRAFVSHLDKHFEPYRPRAEGKSRW
>Mature_305_residues
SSVSDVAFFMEVVKAGTLSGAAQELGVSTAAVSRRLANLEVRLGIRLLNRTTRRAAVTYEGELYLAEGKKILGELEELEQ
RLASAHSVPKGLLRVNATFGFGRQFIAPAIADFVELHPDVDVQLSLSDRPVNLIEDGFDVCIRFGDLPDARLTSRKIACN
RRLLCASPGYLERFGIPASPMDLQRHRCIVIRESDETYGAWHLYSGAAQANVKVRGTVSTNDGEVAVDWALRGLGILLRS
EWNVAPYLRSGRLREVLGDWRFPPADIHAVYPMKSNLSAKVRAFVSHLDKHFEPYRPRAEGKSRW

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789440, Length=285, Percent_Identity=38.9473684210526, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI87081978, Length=295, Percent_Identity=39.3220338983051, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1786401, Length=290, Percent_Identity=34.8275862068965, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=34.8122866894198, Blast_Score=164, Evalue=6e-42,
Organism=Escherichia coli, GI1787128, Length=288, Percent_Identity=29.5138888888889, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1789639, Length=299, Percent_Identity=28.4280936454849, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1787589, Length=288, Percent_Identity=24.6527777777778, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788706, Length=290, Percent_Identity=24.4827586206897, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789173, Length=242, Percent_Identity=28.099173553719, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1786448, Length=240, Percent_Identity=25.8333333333333, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790011, Length=251, Percent_Identity=24.3027888446215, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI157672245, Length=143, Percent_Identity=30.7692307692308, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34030; Mature: 33899

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVSDVAFFMEVVKAGTLSGAAQELGVSTAAVSRRLANLEVRLGIRLLNRTTRRAAVTY
CCCHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHCCHHEEHHHHHHCCCHHEEEEE
EGELYLAEGKKILGELEELEQRLASAHSVPKGLLRVNATFGFGRQFIAPAIADFVELHPD
CCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCC
VDVQLSLSDRPVNLIEDGFDVCIRFGDLPDARLTSRKIACNRRLLCASPGYLERFGIPAS
CCEEEEECCCCCHHHHCCCEEEEEECCCCCCHHHHHHHHHCCEEEECCCCHHHHCCCCCC
PMDLQRHRCIVIRESDETYGAWHLYSGAAQANVKVRGTVSTNDGEVAVDWALRGLGILLR
CCCCCCCEEEEEEECCCCCCEEEEECCCCCCEEEEEEEEECCCCCEEEEEHHHHHHEEEE
SEWNVAPYLRSGRLREVLGDWRFPPADIHAVYPMKSNLSAKVRAFVSHLDKHFEPYRPRA
CCCCCCHHHHCCCHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCC
EGKSRW
CCCCCC
>Mature Secondary Structure 
SSVSDVAFFMEVVKAGTLSGAAQELGVSTAAVSRRLANLEVRLGIRLLNRTTRRAAVTY
CCHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHCCHHEEHHHHHHCCCHHEEEEE
EGELYLAEGKKILGELEELEQRLASAHSVPKGLLRVNATFGFGRQFIAPAIADFVELHPD
CCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCC
VDVQLSLSDRPVNLIEDGFDVCIRFGDLPDARLTSRKIACNRRLLCASPGYLERFGIPAS
CCEEEEECCCCCHHHHCCCEEEEEECCCCCCHHHHHHHHHCCEEEECCCCHHHHCCCCCC
PMDLQRHRCIVIRESDETYGAWHLYSGAAQANVKVRGTVSTNDGEVAVDWALRGLGILLR
CCCCCCCEEEEEEECCCCCCEEEEECCCCCCEEEEEEEEECCCCCEEEEEHHHHHHEEEE
SEWNVAPYLRSGRLREVLGDWRFPPADIHAVYPMKSNLSAKVRAFVSHLDKHFEPYRPRA
CCCCCCHHHHCCCHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCC
EGKSRW
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]