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The map label for this gene is rpoE [H]

Identifier: 34497513

GI number: 34497513

Start: 2246494

End: 2247093

Strand: Direct

Name: rpoE [H]

Synonym: CV_2058

Alternate gene names: 34497513

Gene position: 2246494-2247093 (Clockwise)

Preceding gene: 34497506

Following gene: 34497514

Centisome position: 47.28

GC content: 56.33

Gene sequence:

>600_bases
ATGAGTGATCGGGACATCGATCAACAGTTGGTGGAACGTGCCCAGCGAGGTGAAAAAAGAGCTTTCGATCTGCTTGTTGC
CAAGTATCAGCGACGTCTCGCCAGGCTGTTGTCGCGTTTCATACGCGACGGTTCTGACATCGAGGATGTGACGCAGGAAG
CGTTTATCAAAGCGTATCGCGCCTTGCCGTCGTTCCGAGGTGAGAGCGCGTTTTACACATGGCTGTACAGGATCGGCATC
AATACGGCCAAAAATTTCCTCAGCGCCAACGGACGTCGTCCGGTGGTGAACAGCGAGATCGAGGACGAGGACGGCGAGAG
TTTCGACATGGCGTCGCAAATTCCCGACCTGAACACGCCCGAAACCGAGCTGATGAACAAGGAGATTCTGGCTACGGTCA
ATGCCGCCGTGGAGGCCTTGCCGGAGGAGTTGCGTACTGCCATCTCGCTTAGGGAGATGGAAGGGCTGTCCTACGATGAG
ATCGCCAGGGTGATGGATTGCCCGATAGGTACCGTGCGTTCGCGGATTTTCCGCGCACGCGAGGCGATTGCTGCTGAGCT
CAGGCCTTTGCTGGATACGGCCAAGAACAGAAGGTGGTAA

Upstream 100 bases:

>100_bases
CCTCGCCAGTGGGAACTCGGGCATTAAGCCGTGGTCTAGAACACAGTCCACGTAACGACCACATAGAACAGTGGGCAAAC
CAGGGAGCATAACAACGACT

Downstream 100 bases:

>100_bases
GTATGAAAGAAACGATATCCGCACTGATGGACGGCGAACTGGACGGGCCCGATGCCGACAAGGCAATCGCCGCCATCGCC
GGAGACAAGGACCTGGATGA

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MSDRDIDQQLVERAQRGEKRAFDLLVAKYQRRLARLLSRFIRDGSDIEDVTQEAFIKAYRALPSFRGESAFYTWLYRIGI
NTAKNFLSANGRRPVVNSEIEDEDGESFDMASQIPDLNTPETELMNKEILATVNAAVEALPEELRTAISLREMEGLSYDE
IARVMDCPIGTVRSRIFRAREAIAAELRPLLDTAKNRRW

Sequences:

>Translated_199_residues
MSDRDIDQQLVERAQRGEKRAFDLLVAKYQRRLARLLSRFIRDGSDIEDVTQEAFIKAYRALPSFRGESAFYTWLYRIGI
NTAKNFLSANGRRPVVNSEIEDEDGESFDMASQIPDLNTPETELMNKEILATVNAAVEALPEELRTAISLREMEGLSYDE
IARVMDCPIGTVRSRIFRAREAIAAELRPLLDTAKNRRW
>Mature_198_residues
SDRDIDQQLVERAQRGEKRAFDLLVAKYQRRLARLLSRFIRDGSDIEDVTQEAFIKAYRALPSFRGESAFYTWLYRIGIN
TAKNFLSANGRRPVVNSEIEDEDGESFDMASQIPDLNTPETELMNKEILATVNAAVEALPEELRTAISLREMEGLSYDEI
ARVMDCPIGTVRSRIFRAREAIAAELRPLLDTAKNRRW

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=191, Percent_Identity=55.4973821989529, Blast_Score=224, Evalue=3e-60,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22817; Mature: 22686

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRDIDQQLVERAQRGEKRAFDLLVAKYQRRLARLLSRFIRDGSDIEDVTQEAFIKAYR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ALPSFRGESAFYTWLYRIGINTAKNFLSANGRRPVVNSEIEDEDGESFDMASQIPDLNTP
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCC
ETELMNKEILATVNAAVEALPEELRTAISLREMEGLSYDEIARVMDCPIGTVRSRIFRAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHH
EAIAAELRPLLDTAKNRRW
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDRDIDQQLVERAQRGEKRAFDLLVAKYQRRLARLLSRFIRDGSDIEDVTQEAFIKAYR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ALPSFRGESAFYTWLYRIGINTAKNFLSANGRRPVVNSEIEDEDGESFDMASQIPDLNTP
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCC
ETELMNKEILATVNAAVEALPEELRTAISLREMEGLSYDEIARVMDCPIGTVRSRIFRAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHH
EAIAAELRPLLDTAKNRRW
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]