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The map label for this gene is purU [H]

Identifier: 34497381

GI number: 34497381

Start: 2096121

End: 2096990

Strand: Direct

Name: purU [H]

Synonym: CV_1926

Alternate gene names: 34497381

Gene position: 2096121-2096990 (Clockwise)

Preceding gene: 34497380

Following gene: 34497382

Centisome position: 44.12

GC content: 61.38

Gene sequence:

>870_bases
TTGCTTATGAGTCACGACAAGCATTCGGCGACGCTGTTGATCAGCGCGCCGGACAAGAAGGGCCTGGTAGCGGCCATCGC
CAATTTCCTGATGACTTACAACGCCAACATCATGCATGCCGACCAGCATCAGGACACCAGCGAGAACCTGTTCCTGATGC
GGGTGCAGTGGGATCTGGACGGTTTCACGCTGCCGATGGACAGCTTCGCCGCCGCTTTCCAGCCCATCGCCGACGAGCAC
GGCATGAACTGGAAGGTGTCGCTGTCCGCGCGCAAGCCGCGGATGGCCATCTTCGTGTCGCAGTACGAGCATTGCCTGGT
CGACTTGATGCACCGCTGGCGCATCGGCGAGCTGGATTGCGACATCCCGCTGGTGATTTCCAATCACGAAACCTGCCGCC
GCCTGGTGGAGTTCAACGGCATTCCGTTCCACGTGATCAAGGTCACCAAGGACAACAAGGCCGAGGCCGAGGCCGAGCAG
TTCCGCCTGCTGGAAGAGGCGGGCGTCGATTTCATCGTGCTGGCGCGCTATATGCAGATTCTGTCCGGCGAATTCGTCGA
GCGCTATCCGGACCGCGTGATCAACATCCACCACAGCTTCCTGCCGGCTTTCGACGGCGCCAAACCCTATCACCGCGCTT
TCGCGCGCGGCGTGAAGCTGATCGGCGCCACCAGCCACTACGTGACCGAGGATCTGGACGAAGGCCCGATCATCGAGCAG
GAGGTGACCCGCATCTCGCACCGCGACACGGTGGAGGACCTGGTGGAGAAGGGCCGCGACCTGGAGAAGGTGGTGCTGTC
CCGCGCCGTGCGCTGGCATCTGGACAATCGCGTGCTGTCTTACAGCAACAAAACCGTGGTTTTCGACTGA

Upstream 100 bases:

>100_bases
TTGTTTTCCGCGGCGGCGGCCTGGTCTTGATCATGCCGCCGCATGAATTTGCAGACAGCTGCGCGGCTGATTTAGTATTA
CTTGAAATTAATTTACGGAT

Downstream 100 bases:

>100_bases
GGATGCCATGCGCCTGTTGATCGACGATGTGCTCGATTTGCTGCGCCTGCGGGTCAAGCCGCTGGCGGCCTACCAGTACG
TCTGGTGGCAGCCCGCGCTG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MLMSHDKHSATLLISAPDKKGLVAAIANFLMTYNANIMHADQHQDTSENLFLMRVQWDLDGFTLPMDSFAAAFQPIADEH
GMNWKVSLSARKPRMAIFVSQYEHCLVDLMHRWRIGELDCDIPLVISNHETCRRLVEFNGIPFHVIKVTKDNKAEAEAEQ
FRLLEEAGVDFIVLARYMQILSGEFVERYPDRVINIHHSFLPAFDGAKPYHRAFARGVKLIGATSHYVTEDLDEGPIIEQ
EVTRISHRDTVEDLVEKGRDLEKVVLSRAVRWHLDNRVLSYSNKTVVFD

Sequences:

>Translated_289_residues
MLMSHDKHSATLLISAPDKKGLVAAIANFLMTYNANIMHADQHQDTSENLFLMRVQWDLDGFTLPMDSFAAAFQPIADEH
GMNWKVSLSARKPRMAIFVSQYEHCLVDLMHRWRIGELDCDIPLVISNHETCRRLVEFNGIPFHVIKVTKDNKAEAEAEQ
FRLLEEAGVDFIVLARYMQILSGEFVERYPDRVINIHHSFLPAFDGAKPYHRAFARGVKLIGATSHYVTEDLDEGPIIEQ
EVTRISHRDTVEDLVEKGRDLEKVVLSRAVRWHLDNRVLSYSNKTVVFD
>Mature_289_residues
MLMSHDKHSATLLISAPDKKGLVAAIANFLMTYNANIMHADQHQDTSENLFLMRVQWDLDGFTLPMDSFAAAFQPIADEH
GMNWKVSLSARKPRMAIFVSQYEHCLVDLMHRWRIGELDCDIPLVISNHETCRRLVEFNGIPFHVIKVTKDNKAEAEAEQ
FRLLEEAGVDFIVLARYMQILSGEFVERYPDRVINIHHSFLPAFDGAKPYHRAFARGVKLIGATSHYVTEDLDEGPIIEQ
EVTRISHRDTVEDLVEKGRDLEKVVLSRAVRWHLDNRVLSYSNKTVVFD

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=206, Percent_Identity=33.495145631068, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI209869995, Length=206, Percent_Identity=33.495145631068, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI209869993, Length=206, Percent_Identity=33.495145631068, Blast_Score=105, Evalue=3e-23,
Organism=Escherichia coli, GI1787483, Length=284, Percent_Identity=41.5492957746479, Blast_Score=216, Evalue=1e-57,
Organism=Escherichia coli, GI1788846, Length=145, Percent_Identity=29.6551724137931, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=175, Percent_Identity=34.8571428571429, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24582400, Length=198, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 33158; Mature: 33158

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMSHDKHSATLLISAPDKKGLVAAIANFLMTYNANIMHADQHQDTSENLFLMRVQWDLD
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEEECCC
GFTLPMDSFAAAFQPIADEHGMNWKVSLSARKPRMAIFVSQYEHCLVDLMHRWRIGELDC
CEEECHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCC
DIPLVISNHETCRRLVEFNGIPFHVIKVTKDNKAEAEAEQFRLLEEAGVDFIVLARYMQI
CCEEEECCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
LSGEFVERYPDRVINIHHSFLPAFDGAKPYHRAFARGVKLIGATSHYVTEDLDEGPIIEQ
HCCHHHHHCCHHHEEHHHHHCCCCCCCCHHHHHHHHHHHEEECCHHHHHCCCCCCCCHHH
EVTRISHRDTVEDLVEKGRDLEKVVLSRAVRWHLDNRVLSYSNKTVVFD
HHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEECCCEEEEC
>Mature Secondary Structure
MLMSHDKHSATLLISAPDKKGLVAAIANFLMTYNANIMHADQHQDTSENLFLMRVQWDLD
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEEECCC
GFTLPMDSFAAAFQPIADEHGMNWKVSLSARKPRMAIFVSQYEHCLVDLMHRWRIGELDC
CEEECHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCC
DIPLVISNHETCRRLVEFNGIPFHVIKVTKDNKAEAEAEQFRLLEEAGVDFIVLARYMQI
CCEEEECCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
LSGEFVERYPDRVINIHHSFLPAFDGAKPYHRAFARGVKLIGATSHYVTEDLDEGPIIEQ
HCCHHHHHCCHHHEEHHHHHCCCCCCCCHHHHHHHHHHHEEECCHHHHHCCCCCCCCHHH
EVTRISHRDTVEDLVEKGRDLEKVVLSRAVRWHLDNRVLSYSNKTVVFD
HHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]