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The map label for this gene is folD

Identifier: 34497380

GI number: 34497380

Start: 2095095

End: 2095946

Strand: Direct

Name: folD

Synonym: CV_1925

Alternate gene names: 34497380

Gene position: 2095095-2095946 (Clockwise)

Preceding gene: 34497379

Following gene: 34497381

Centisome position: 44.1

GC content: 65.14

Gene sequence:

>852_bases
ATGTCGGCACAATTGATCGATGGCAAGGCGGTAGCGGAAAAACTGCTGGAAAAGGTAAGCGCGGGCGTGGGCGAGCGGTT
GGCCCAAGGCAAGCGGGCGCCGGCGCTGGCGGTGATCCTGGTCGGCGATAATCCGGCCTCCGCGGTTTACGTGGGCAGCA
AGAAGAAGGCTTGCGAAAAGGCGGGCATTCGTTCCGTGGCCTATGACCTGCCGGCGTCCACCAGCCAGCAGCAGCTGCTG
GAAATCATCGACGGCCTGAATGCGGACGACGCCGTGGACGGCATCCTGGTGCAATTGCCGCTGCCGGAGCAGATCGACCC
GCAGGCGGTGATCGAGCGCATCGATCCGAAAAAGGACGTGGACGGCTTCCACCCTTATAATGTCGGCCGGCTGGCGATCA
AGATGCCGCTGATGCGGCCGTGCACGCCGCGCGGCGTGATGACGCTGCTGGAAGAATACGGCATCGATCCCAAGGGCAAA
AAGGCAGTGATCGTCGGCGCGTCCAACATCGTCGGCCGCCCGCAGGCGCTGGAGATGCTGCTGGCGCGAGCCACCGTCAC
CGTTTGCCACAGCGCCACGCGCAATCTGGCGGAGGAGGTGGCCGCCGCCGACATCCTGGTGGTGGGCGTGGGCATTCCCA
ACTTCGTCAAGGGCGAGTGGGTCAAGCCGGGCGCGGTGGTGATCGACGTGGGCATCAACCGGCTGGATACCGGCAAGCTG
TGCGGCGACGTGGAATTCGACGCGGCGCGCGAACGCGCCGGCTTCATTACCCCGGTGCCGGGCGGCGTGGGGCCGATGAC
GGTGGCGACCTTGTTGCAGAATACGCTGGATTCCGCCAATCTGAACGCCTGA

Upstream 100 bases:

>100_bases
TGATCTTAAGAAAAATAATCGGCGGAGCGCTCTTACTGCGCTGCGCGATTAATGCGAGAATAGCGGCCGTTATTTACCTG
CATCAATATGGGAATCAGGC

Downstream 100 bases:

>100_bases
GCAGGACGCCGCGTTCGCGCGGCGGCCGCAGCCAAGCTCGCCACAGCGGCGCGACGCGCCTGGCTGTGGCGATTTTGTTT
TCCGCGGCGGCGGCCTGGTC

Product: methylenetetrahydrofolate dehydrogenase/cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSAQLIDGKAVAEKLLEKVSAGVGERLAQGKRAPALAVILVGDNPASAVYVGSKKKACEKAGIRSVAYDLPASTSQQQLL
EIIDGLNADDAVDGILVQLPLPEQIDPQAVIERIDPKKDVDGFHPYNVGRLAIKMPLMRPCTPRGVMTLLEEYGIDPKGK
KAVIVGASNIVGRPQALEMLLARATVTVCHSATRNLAEEVAAADILVVGVGIPNFVKGEWVKPGAVVIDVGINRLDTGKL
CGDVEFDAARERAGFITPVPGGVGPMTVATLLQNTLDSANLNA

Sequences:

>Translated_283_residues
MSAQLIDGKAVAEKLLEKVSAGVGERLAQGKRAPALAVILVGDNPASAVYVGSKKKACEKAGIRSVAYDLPASTSQQQLL
EIIDGLNADDAVDGILVQLPLPEQIDPQAVIERIDPKKDVDGFHPYNVGRLAIKMPLMRPCTPRGVMTLLEEYGIDPKGK
KAVIVGASNIVGRPQALEMLLARATVTVCHSATRNLAEEVAAADILVVGVGIPNFVKGEWVKPGAVVIDVGINRLDTGKL
CGDVEFDAARERAGFITPVPGGVGPMTVATLLQNTLDSANLNA
>Mature_282_residues
SAQLIDGKAVAEKLLEKVSAGVGERLAQGKRAPALAVILVGDNPASAVYVGSKKKACEKAGIRSVAYDLPASTSQQQLLE
IIDGLNADDAVDGILVQLPLPEQIDPQAVIERIDPKKDVDGFHPYNVGRLAIKMPLMRPCTPRGVMTLLEEYGIDPKGKK
AVIVGASNIVGRPQALEMLLARATVTVCHSATRNLAEEVAAADILVVGVGIPNFVKGEWVKPGAVVIDVGINRLDTGKLC
GDVEFDAARERAGFITPVPGGVGPMTVATLLQNTLDSANLNA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=288, Percent_Identity=44.7916666666667, Blast_Score=231, Evalue=5e-61,
Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=45.9183673469388, Blast_Score=224, Evalue=5e-59,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=45.0847457627119, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI36796743, Length=213, Percent_Identity=28.6384976525822, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=60.9318996415771, Blast_Score=357, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17568735, Length=291, Percent_Identity=43.9862542955326, Blast_Score=216, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6319558, Length=285, Percent_Identity=46.3157894736842, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=43.9597315436242, Blast_Score=229, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6322933, Length=313, Percent_Identity=27.4760383386581, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI17136816, Length=293, Percent_Identity=47.7815699658703, Blast_Score=241, Evalue=4e-64,
Organism=Drosophila melanogaster, GI17136818, Length=293, Percent_Identity=47.7815699658703, Blast_Score=240, Evalue=6e-64,
Organism=Drosophila melanogaster, GI62472483, Length=287, Percent_Identity=45.993031358885, Blast_Score=226, Evalue=1e-59,
Organism=Drosophila melanogaster, GI45551871, Length=287, Percent_Identity=45.993031358885, Blast_Score=226, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24645718, Length=287, Percent_Identity=45.993031358885, Blast_Score=226, Evalue=1e-59,
Organism=Drosophila melanogaster, GI17137370, Length=287, Percent_Identity=45.993031358885, Blast_Score=226, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_CHRVO (Q7NWQ6)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901595.1
- HSSP:   P11586
- ProteinModelPortal:   Q7NWQ6
- SMR:   Q7NWQ6
- GeneID:   2548915
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1925
- NMPDR:   fig|243365.1.peg.1925
- HOGENOM:   HBG328751
- OMA:   DVVYETA
- PhylomeDB:   Q7NWQ6
- BioCyc:   CVIO243365:CV_1925-MONOMER
- BRENDA:   1.5.1.5
- BRENDA:   3.5.4.9
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 29697; Mature: 29566

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQLIDGKAVAEKLLEKVSAGVGERLAQGKRAPALAVILVGDNPASAVYVGSKKKACEK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEECCCHHHHHH
AGIRSVAYDLPASTSQQQLLEIIDGLNADDAVDGILVQLPLPEQIDPQAVIERIDPKKDV
HCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCC
DGFHPYNVGRLAIKMPLMRPCTPRGVMTLLEEYGIDPKGKKAVIVGASNIVGRPQALEML
CCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHH
LARATVTVCHSATRNLAEEVAAADILVVGVGIPNFVKGEWVKPGAVVIDVGINRLDTGKL
HHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
CGDVEFDAARERAGFITPVPGGVGPMTVATLLQNTLDSANLNA
CCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SAQLIDGKAVAEKLLEKVSAGVGERLAQGKRAPALAVILVGDNPASAVYVGSKKKACEK
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEECCCHHHHHH
AGIRSVAYDLPASTSQQQLLEIIDGLNADDAVDGILVQLPLPEQIDPQAVIERIDPKKDV
HCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCC
DGFHPYNVGRLAIKMPLMRPCTPRGVMTLLEEYGIDPKGKKAVIVGASNIVGRPQALEML
CCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHH
LARATVTVCHSATRNLAEEVAAADILVVGVGIPNFVKGEWVKPGAVVIDVGINRLDTGKL
HHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
CGDVEFDAARERAGFITPVPGGVGPMTVATLLQNTLDSANLNA
CCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782