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The map label for this gene is hmp [H]

Identifier: 34497229

GI number: 34497229

Start: 1929510

End: 1931492

Strand: Reverse

Name: hmp [H]

Synonym: CV_1774

Alternate gene names: 34497229

Gene position: 1931492-1929510 (Counterclockwise)

Preceding gene: 34497230

Following gene: 34497227

Centisome position: 40.65

GC content: 71.46

Gene sequence:

>1983_bases
ATGTCCGACTCCCCTCCCATCCCCTGGCACTCCGGCGAACGGGAAATGCAACGCCGCGCCGGCAGCCTGGAGCACATGGC
GGCGACAGGCCCACGCGTGGTGCGCGACCACATGCCGGAACAGCATCGCGACTTCTTCCGCCAACTGCCCTTCATGGTGA
TGGCGGCGGTGGACGAGGCCGGCAGGCCGTGGGCGGGAATCGTCGAGGGGCGCCCCGGCTTCGTCGACTCGCCCGATCCC
CGCAGCCTGCGCATCGCCGCGCAGCCCAGTCCCGCCGACCCGCTGCGCGACTGCCTGCGTCCCGGCGCGGCGGTGGGCCT
GCTCGGCATCGAGCTGCATACCCGCCGCCGCAACCGGATGAACGGCGAACTGACGGCGATGGACGACGGCGGCTTCGCCG
TCGCCGTCGGCCAATCGTTCGGCAACTGCCCCAAGTACATCCAGCTGCGCGAGTTCGAATTCTCCCGCGAGCCGGGTCCG
CGCATCCTGGGTTCGGTCGAGTGGATGGACGAGCTTGACGACGACGCGCGCGCCGCCATCGCCGCCGCCGACACCTTCTT
CGTCGCCAGCGCCGTTCAAGACAGCCATGGCCGCTGGCAGGCCGACGCCTCGCACCGCGGCGGCAAGCCCGGCTTCGTCA
AGATGGATGGCGACACGCTGACCATCCCCGACTTCGCCGGCAACGGCTATTTCAACACCCTGGGCAATCTGCTGCTGCAG
CCGCGCGCCGGCCTGCTGTTCGTCGATTTCGCCAGCGGCGACACGCTGCAGCTGGCCGGCCGCGCCGAGGTGCCGGACAC
CGAGACCCCGCCGCCGTTCGCCGGCGCTGAGCGCTGGTGGACCTTTCGCGTCGAGCGCGTGGTCCGCCGCCGCAACGCCT
TGGCCCTGCGCTGGACGCTGCGCGAATACTCGCCGTTCGCGCTCGCCACCGGCGCCTGGCCGCACGCCGCGCCGGAGCGG
CAATGGCTGCCGCTGCGGGTGGTCCATGCCGAGGACGAGAGCGACGCCGTCCGCTCCATTTATCTTGAGCCGGCCGACGG
CTCGGCCCCGCCGCCCTTCCTGCCCGGCCAGCACCTGAGCCTGAAAGTCGCGGGCGTGGACGGCGTGCGGATGCGCAACT
ACACGCTGTCGCAAACCGGCGGCTACCGCATCAGCGTCAAGCGCCAGGGCAAGGCGTCCGCCCGGCTGCACCAGCTGGCG
CCGGGCGACATCGTCGAGGCGCTGCCGCCGCGCGGCGACTTCACGCTGGCGCGCGCCGACCGCCCGATCGCGCTGCTGGC
CGGCGGCATCGGCATCACCCCGCTGCTGGCGATGCTGCACCAGCTGACGGCGCGTCCCGCCGCCATGCCGCCCACGCTGC
TGGCCTACGCCACCCGCACCATCGCCGAGCGCGCGTTCGACGCCGAGCTGGAAGCGCTGCAGGCCAAGGCCGCCGGCCGG
CTGCGCATCGTCAAGGCGGCGAGCCAGCCGGAAACCGGACGCCGCCTCGGCGTCGACTACCAGCATGCCGGCCATGTCGA
TATCGACCTGCTGCGCCGCAATGGCCTGAGCCTGGATGGGGACTTTTATCTGTGCGGCCCGGCCGGCTTCATGCAAGCGC
TGTACGAACAGTTGATCGCCGCGGGCGTGGACGATAAGCGCATCCACGCCGAAGCCTTCGGCCCGGCCGGTATCCAACGG
ATCGGCCAGGTCGCGGGCAAGCGGCCGCCGCCGGCCGACCATGCCGTGCCGGTGCGTTTCTCGGCATCGTCAATCGACGC
CGAATGGCGGCCGGGCCAGAGCCTGCTGGAGCTGGCCGAGTCCTGCGGCCTGAACCCCGACTTCAGCTGCCGCGGCGGCG
CTTGCGGCAGTTGCCGCGCCGCCTTGCTGTCCGGCGAGGCGACCTATCTGCAGCCGCCGGAATACGCCGCCCGATCCGGG
GAGATTCTGCTGTGCTGCGCCTACCCGGCCGAAGGCTCGGGCAAGCTGGAAATCAACCTGTAA

Upstream 100 bases:

>100_bases
GCGCCGTATCCGCATGTCCGCGCCTGGCTGGCCCGCGTCGAGGCGCTGCCCGGCTTCGTGGCGATGCCGCCCGCCAACGC
TTAAATGACAGGAACCGCGA

Downstream 100 bases:

>100_bases
GCCGCGGGTCGGCGCGCAGCCGCTCGGCCAGAAAATCGATGAAGCGGCGCATCCGCGTCGAGCCGTGGCGGCTTTCCGCG
TGGACGATGTGGATGGGCAA

Product: flavohemoprotein

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 660; Mature: 659

Protein sequence:

>660_residues
MSDSPPIPWHSGEREMQRRAGSLEHMAATGPRVVRDHMPEQHRDFFRQLPFMVMAAVDEAGRPWAGIVEGRPGFVDSPDP
RSLRIAAQPSPADPLRDCLRPGAAVGLLGIELHTRRRNRMNGELTAMDDGGFAVAVGQSFGNCPKYIQLREFEFSREPGP
RILGSVEWMDELDDDARAAIAAADTFFVASAVQDSHGRWQADASHRGGKPGFVKMDGDTLTIPDFAGNGYFNTLGNLLLQ
PRAGLLFVDFASGDTLQLAGRAEVPDTETPPPFAGAERWWTFRVERVVRRRNALALRWTLREYSPFALATGAWPHAAPER
QWLPLRVVHAEDESDAVRSIYLEPADGSAPPPFLPGQHLSLKVAGVDGVRMRNYTLSQTGGYRISVKRQGKASARLHQLA
PGDIVEALPPRGDFTLARADRPIALLAGGIGITPLLAMLHQLTARPAAMPPTLLAYATRTIAERAFDAELEALQAKAAGR
LRIVKAASQPETGRRLGVDYQHAGHVDIDLLRRNGLSLDGDFYLCGPAGFMQALYEQLIAAGVDDKRIHAEAFGPAGIQR
IGQVAGKRPPPADHAVPVRFSASSIDAEWRPGQSLLELAESCGLNPDFSCRGGACGSCRAALLSGEATYLQPPEYAARSG
EILLCCAYPAEGSGKLEINL

Sequences:

>Translated_660_residues
MSDSPPIPWHSGEREMQRRAGSLEHMAATGPRVVRDHMPEQHRDFFRQLPFMVMAAVDEAGRPWAGIVEGRPGFVDSPDP
RSLRIAAQPSPADPLRDCLRPGAAVGLLGIELHTRRRNRMNGELTAMDDGGFAVAVGQSFGNCPKYIQLREFEFSREPGP
RILGSVEWMDELDDDARAAIAAADTFFVASAVQDSHGRWQADASHRGGKPGFVKMDGDTLTIPDFAGNGYFNTLGNLLLQ
PRAGLLFVDFASGDTLQLAGRAEVPDTETPPPFAGAERWWTFRVERVVRRRNALALRWTLREYSPFALATGAWPHAAPER
QWLPLRVVHAEDESDAVRSIYLEPADGSAPPPFLPGQHLSLKVAGVDGVRMRNYTLSQTGGYRISVKRQGKASARLHQLA
PGDIVEALPPRGDFTLARADRPIALLAGGIGITPLLAMLHQLTARPAAMPPTLLAYATRTIAERAFDAELEALQAKAAGR
LRIVKAASQPETGRRLGVDYQHAGHVDIDLLRRNGLSLDGDFYLCGPAGFMQALYEQLIAAGVDDKRIHAEAFGPAGIQR
IGQVAGKRPPPADHAVPVRFSASSIDAEWRPGQSLLELAESCGLNPDFSCRGGACGSCRAALLSGEATYLQPPEYAARSG
EILLCCAYPAEGSGKLEINL
>Mature_659_residues
SDSPPIPWHSGEREMQRRAGSLEHMAATGPRVVRDHMPEQHRDFFRQLPFMVMAAVDEAGRPWAGIVEGRPGFVDSPDPR
SLRIAAQPSPADPLRDCLRPGAAVGLLGIELHTRRRNRMNGELTAMDDGGFAVAVGQSFGNCPKYIQLREFEFSREPGPR
ILGSVEWMDELDDDARAAIAAADTFFVASAVQDSHGRWQADASHRGGKPGFVKMDGDTLTIPDFAGNGYFNTLGNLLLQP
RAGLLFVDFASGDTLQLAGRAEVPDTETPPPFAGAERWWTFRVERVVRRRNALALRWTLREYSPFALATGAWPHAAPERQ
WLPLRVVHAEDESDAVRSIYLEPADGSAPPPFLPGQHLSLKVAGVDGVRMRNYTLSQTGGYRISVKRQGKASARLHQLAP
GDIVEALPPRGDFTLARADRPIALLAGGIGITPLLAMLHQLTARPAAMPPTLLAYATRTIAERAFDAELEALQAKAAGRL
RIVKAASQPETGRRLGVDYQHAGHVDIDLLRRNGLSLDGDFYLCGPAGFMQALYEQLIAAGVDDKRIHAEAFGPAGIQRI
GQVAGKRPPPADHAVPVRFSASSIDAEWRPGQSLLELAESCGLNPDFSCRGGACGSCRAALLSGEATYLQPPEYAARSGE
ILLCCAYPAEGSGKLEINL

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG3576

COG function: function code R; Predicted flavin-nucleotide-binding protein structurally related to pyridoxine 5'-phosphate oxidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=243, Percent_Identity=35.3909465020576, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787658, Length=358, Percent_Identity=32.4022346368715, Blast_Score=96, Evalue=7e-21,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 71546; Mature: 71415

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSPPIPWHSGEREMQRRAGSLEHMAATGPRVVRDHMPEQHRDFFRQLPFMVMAAVDEA
CCCCCCCCCCCCHHHHHHHCCCHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
GRPWAGIVEGRPGFVDSPDPRSLRIAAQPSPADPLRDCLRPGAAVGLLGIELHTRRRNRM
CCCCEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCEEEEEEEEEEHHHHHCC
NGELTAMDDGGFAVAVGQSFGNCPKYIQLREFEFSREPGPRILGSVEWMDELDDDARAAI
CCEEEEECCCCEEEEECCCCCCCCCEEEEEEEECCCCCCCHHEECHHHHHHCCCHHHHHH
AAADTFFVASAVQDSHGRWQADASHRGGKPGFVKMDGDTLTIPDFAGNGYFNTLGNLLLQ
HHHHHHHHHHHHHCCCCCEECCCCCCCCCCCEEEECCCEEECCCCCCCCHHHHHHHHHCC
PRAGLLFVDFASGDTLQLAGRAEVPDTETPPPFAGAERWWTFRVERVVRRRNALALRWTL
CCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEE
REYSPFALATGAWPHAAPERQWLPLRVVHAEDESDAVRSIYLEPADGSAPPPFLPGQHLS
CCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCEE
LKVAGVDGVRMRNYTLSQTGGYRISVKRQGKASARLHQLAPGDIVEALPPRGDFTLARAD
EEEECCCCEEEECEEEECCCCEEEEEECCCCCHHHHHHCCCCHHHHHCCCCCCEEEEECC
RPIALLAGGIGITPLLAMLHQLTARPAAMPPTLLAYATRTIAERAFDAELEALQAKAAGR
CCEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LRIVKAASQPETGRRLGVDYQHAGHVDIDLLRRNGLSLDGDFYLCGPAGFMQALYEQLIA
EEEEEECCCCCCCCCCCCCCCCCCCCEEEEHHCCCCCCCCCEEEECCHHHHHHHHHHHHH
AGVDDKRIHAEAFGPAGIQRIGQVAGKRPPPADHAVPVRFSASSIDAEWRPGQSLLELAE
CCCCCCEEEHHHCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHH
SCGLNPDFSCRGGACGSCRAALLSGEATYLQPPEYAARSGEILLCCAYPAEGSGKLEINL
HCCCCCCCCCCCCCCCHHHHHHCCCCCCEECCCHHHCCCCCEEEEEECCCCCCCEEEEEC
>Mature Secondary Structure 
SDSPPIPWHSGEREMQRRAGSLEHMAATGPRVVRDHMPEQHRDFFRQLPFMVMAAVDEA
CCCCCCCCCCCHHHHHHHCCCHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
GRPWAGIVEGRPGFVDSPDPRSLRIAAQPSPADPLRDCLRPGAAVGLLGIELHTRRRNRM
CCCCEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCEEEEEEEEEEHHHHHCC
NGELTAMDDGGFAVAVGQSFGNCPKYIQLREFEFSREPGPRILGSVEWMDELDDDARAAI
CCEEEEECCCCEEEEECCCCCCCCCEEEEEEEECCCCCCCHHEECHHHHHHCCCHHHHHH
AAADTFFVASAVQDSHGRWQADASHRGGKPGFVKMDGDTLTIPDFAGNGYFNTLGNLLLQ
HHHHHHHHHHHHHCCCCCEECCCCCCCCCCCEEEECCCEEECCCCCCCCHHHHHHHHHCC
PRAGLLFVDFASGDTLQLAGRAEVPDTETPPPFAGAERWWTFRVERVVRRRNALALRWTL
CCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEE
REYSPFALATGAWPHAAPERQWLPLRVVHAEDESDAVRSIYLEPADGSAPPPFLPGQHLS
CCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCEE
LKVAGVDGVRMRNYTLSQTGGYRISVKRQGKASARLHQLAPGDIVEALPPRGDFTLARAD
EEEECCCCEEEECEEEECCCCEEEEEECCCCCHHHHHHCCCCHHHHHCCCCCCEEEEECC
RPIALLAGGIGITPLLAMLHQLTARPAAMPPTLLAYATRTIAERAFDAELEALQAKAAGR
CCEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LRIVKAASQPETGRRLGVDYQHAGHVDIDLLRRNGLSLDGDFYLCGPAGFMQALYEQLIA
EEEEEECCCCCCCCCCCCCCCCCCCCEEEEHHCCCCCCCCCEEEECCHHHHHHHHHHHHH
AGVDDKRIHAEAFGPAGIQRIGQVAGKRPPPADHAVPVRFSASSIDAEWRPGQSLLELAE
CCCCCCEEEHHHCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHH
SCGLNPDFSCRGGACGSCRAALLSGEATYLQPPEYAARSGEILLCCAYPAEGSGKLEINL
HCCCCCCCCCCCCCCCHHHHHHCCCCCCEECCCHHHCCCCCEEEEEECCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]