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The map label for this gene is mpl [H]

Identifier: 34497121

GI number: 34497121

Start: 1805658

End: 1807028

Strand: Direct

Name: mpl [H]

Synonym: CV_1666

Alternate gene names: 34497121

Gene position: 1805658-1807028 (Clockwise)

Preceding gene: 34497116

Following gene: 34497125

Centisome position: 38.01

GC content: 66.96

Gene sequence:

>1371_bases
ATGCACATCCACATCCTGGGTATCTGCGGTACCTTCATGGGCGGCATCGCCGCCATCGCCAAACAGGCCGGCCACAAGGT
GACCGGTTGCGACGCCAATGTCTATCCGCCGATGAGCACCCAGCTGGAGGCGATGGGCATAGAACTGACCCAGGGCTTCG
GCGCGGAGCAGGTGGACCTGAACCCGGACGTGTATGTGATCGGCAATGTGGTGACGCGCGGCAAGCCGCTGATGGAGGAA
ATCCTCAACCGCGGCCTGCCTTATATTTCCGGCCCGCAGTGGCTGGCCGAGAACGTGCTGCGCGACAAATGGGTGCTGGC
GGTGGCCGGCACCCACGGCAAGACCACCACCAGCTCGATGCTGGCGTGGATACTGGAGGACGCCGGCCTGGCGCCCGGCT
TTCTGATCGGCGGCATTCCGGAGAACTTCGGTGTGTCGGCGCGGCTGCCGGGCGCGCCGGCGCTGGATCCGGCGAGCGTC
AGCCCCTTCTTCGTGATCGAGGCCGACGAGTACGACACCGCCTTCTTCGACAAGCGTTCCAAGTTCGTCCATTACCGCCC
GCGCACCGCGATCCTGAACAACCTGGAATTCGACCACGCCGACATCTTCGCCGACCTGGCGGCGATTGAAACCCAGTTCC
ACCACCTGGTGCGCACCATTCCGGGCAACGGCCGCATCGTCAGCAACGGCCGCGAGGCCAATCTGGACCGCGTGCTGGCG
CGCGGCTGCTGGACGCCGGCGGAGCGCTTCGGCGTGGACGCCGGCTGGCAGCTGGGCGCGCTGCACGAGGACGGCGGCTT
CGACGTGCTGCTGAACGGCGAGCAGCAAGGCACGGTGCGCTGGGCGCTGATGGGTGAGCACAACCGGCTGAACGCGATCG
CCGCCATCGCCGCCGCGCGCCATGCCGGCGTCGCGGTCAAGGACGCGATAGACGCCTTGTCCCGTTTCGCCAACGTCAAG
CGCCGGATGGAAGTGAAGGGCGTGGCGGGCGGCGTCACCGTCTACGATGATTTCGCCCACCACCCCACCGCCATCGCCAC
CACGCTGGAAGGCCTGCGCCGCAAAGTGGGCGACGCTCGCATTCTGGCCGTGCTGGAGCCGCGCTCCAACACCATGAAGC
TGGGCACGATGAAGGACGCGCTGCCGGGTTCGCTGGCGCTGGCCGACAAGGTGTTCTGCTCGGCCGCGGGGCTGAACTGG
AATCCGGGCGAGGCGCTGCAAGTCATGGGGGCGAAGGCGCAGACCTTCAGCGATTTCGACGCGCTGCTGGGCGCCATCGC
CGCCGAGGCCAAACCGGGCGACCATGTGCTGGTGATGAGCAACGGCGGCTTCGGCGGCATTCACCAGAAGCTGCTGGACA
AATTGGCCTGA

Upstream 100 bases:

>100_bases
ACGGCATCCGGCCGCTGCCCGGGCGCCAGGGGCCTGGTGTCGGCCCGGGCGGCTTTCCGGTATCCTTGCGGTTTCGTTGT
CTTTAGCTGCGTTGAACGAT

Downstream 100 bases:

>100_bases
GCCGCGCCGCCGGCTTGGCGCATGGATCCGGGGCGTGCGGAGATGCCCCCCCGCCTCAGGGCAGCTGGATGATGTAGTTG
GCGCCGCCGTCGCGCGCCTT

Product: UDP-N-acetylmuramate: L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MHIHILGICGTFMGGIAAIAKQAGHKVTGCDANVYPPMSTQLEAMGIELTQGFGAEQVDLNPDVYVIGNVVTRGKPLMEE
ILNRGLPYISGPQWLAENVLRDKWVLAVAGTHGKTTTSSMLAWILEDAGLAPGFLIGGIPENFGVSARLPGAPALDPASV
SPFFVIEADEYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFADLAAIETQFHHLVRTIPGNGRIVSNGREANLDRVLA
RGCWTPAERFGVDAGWQLGALHEDGGFDVLLNGEQQGTVRWALMGEHNRLNAIAAIAAARHAGVAVKDAIDALSRFANVK
RRMEVKGVAGGVTVYDDFAHHPTAIATTLEGLRRKVGDARILAVLEPRSNTMKLGTMKDALPGSLALADKVFCSAAGLNW
NPGEALQVMGAKAQTFSDFDALLGAIAAEAKPGDHVLVMSNGGFGGIHQKLLDKLA

Sequences:

>Translated_456_residues
MHIHILGICGTFMGGIAAIAKQAGHKVTGCDANVYPPMSTQLEAMGIELTQGFGAEQVDLNPDVYVIGNVVTRGKPLMEE
ILNRGLPYISGPQWLAENVLRDKWVLAVAGTHGKTTTSSMLAWILEDAGLAPGFLIGGIPENFGVSARLPGAPALDPASV
SPFFVIEADEYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFADLAAIETQFHHLVRTIPGNGRIVSNGREANLDRVLA
RGCWTPAERFGVDAGWQLGALHEDGGFDVLLNGEQQGTVRWALMGEHNRLNAIAAIAAARHAGVAVKDAIDALSRFANVK
RRMEVKGVAGGVTVYDDFAHHPTAIATTLEGLRRKVGDARILAVLEPRSNTMKLGTMKDALPGSLALADKVFCSAAGLNW
NPGEALQVMGAKAQTFSDFDALLGAIAAEAKPGDHVLVMSNGGFGGIHQKLLDKLA
>Mature_456_residues
MHIHILGICGTFMGGIAAIAKQAGHKVTGCDANVYPPMSTQLEAMGIELTQGFGAEQVDLNPDVYVIGNVVTRGKPLMEE
ILNRGLPYISGPQWLAENVLRDKWVLAVAGTHGKTTTSSMLAWILEDAGLAPGFLIGGIPENFGVSARLPGAPALDPASV
SPFFVIEADEYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFADLAAIETQFHHLVRTIPGNGRIVSNGREANLDRVLA
RGCWTPAERFGVDAGWQLGALHEDGGFDVLLNGEQQGTVRWALMGEHNRLNAIAAIAAARHAGVAVKDAIDALSRFANVK
RRMEVKGVAGGVTVYDDFAHHPTAIATTLEGLRRKVGDARILAVLEPRSNTMKLGTMKDALPGSLALADKVFCSAAGLNW
NPGEALQVMGAKAQTFSDFDALLGAIAAEAKPGDHVLVMSNGGFGGIHQKLLDKLA

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=459, Percent_Identity=60.3485838779956, Blast_Score=546, Evalue=1e-156,
Organism=Escherichia coli, GI1786279, Length=385, Percent_Identity=30.1298701298701, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 48695; Mature: 48695

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHILGICGTFMGGIAAIAKQAGHKVTGCDANVYPPMSTQLEAMGIELTQGFGAEQVDL
CEEEEEEHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHCEEEECCCCCCEEEC
NPDVYVIGNVVTRGKPLMEEILNRGLPYISGPQWLAENVLRDKWVLAVAGTHGKTTTSSM
CCCEEEEECHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWILEDAGLAPGFLIGGIPENFGVSARLPGAPALDPASVSPFFVIEADEYDTAFFDKRS
HHHHHHHCCCCCCEEECCCCCCCCCEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHCCC
KFVHYRPRTAILNNLEFDHADIFADLAAIETQFHHLVRTIPGNGRIVSNGREANLDRVLA
CEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHH
RGCWTPAERFGVDAGWQLGALHEDGGFDVLLNGEQQGTVRWALMGEHNRLNAIAAIAAAR
HCCCCHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHH
HAGVAVKDAIDALSRFANVKRRMEVKGVAGGVTVYDDFAHHPTAIATTLEGLRRKVGDAR
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCEE
ILAVLEPRSNTMKLGTMKDALPGSLALADKVFCSAAGLNWNPGEALQVMGAKAQTFSDFD
EEEEECCCCCEEEECCHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHH
ALLGAIAAEAKPGDHVLVMSNGGFGGIHQKLLDKLA
HHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCC
>Mature Secondary Structure
MHIHILGICGTFMGGIAAIAKQAGHKVTGCDANVYPPMSTQLEAMGIELTQGFGAEQVDL
CEEEEEEHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHCEEEECCCCCCEEEC
NPDVYVIGNVVTRGKPLMEEILNRGLPYISGPQWLAENVLRDKWVLAVAGTHGKTTTSSM
CCCEEEEECHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWILEDAGLAPGFLIGGIPENFGVSARLPGAPALDPASVSPFFVIEADEYDTAFFDKRS
HHHHHHHCCCCCCEEECCCCCCCCCEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHCCC
KFVHYRPRTAILNNLEFDHADIFADLAAIETQFHHLVRTIPGNGRIVSNGREANLDRVLA
CEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHH
RGCWTPAERFGVDAGWQLGALHEDGGFDVLLNGEQQGTVRWALMGEHNRLNAIAAIAAAR
HCCCCHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHH
HAGVAVKDAIDALSRFANVKRRMEVKGVAGGVTVYDDFAHHPTAIATTLEGLRRKVGDAR
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCEE
ILAVLEPRSNTMKLGTMKDALPGSLALADKVFCSAAGLNWNPGEALQVMGAKAQTFSDFD
EEEEECCCCCEEEECCHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHH
ALLGAIAAEAKPGDHVLVMSNGGFGGIHQKLLDKLA
HHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]