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The map label for this gene is panB

Identifier: 34497090

GI number: 34497090

Start: 1772795

End: 1773583

Strand: Direct

Name: panB

Synonym: CV_1635

Alternate gene names: 34497090

Gene position: 1772795-1773583 (Clockwise)

Preceding gene: 34497089

Following gene: 34497091

Centisome position: 37.31

GC content: 65.65

Gene sequence:

>789_bases
ATGAAAATAACCGTCAATACCCTGCACAAGCTGGCCGAGGAAGGCCGGAAGATCACCATGCTGACCTGCTACGACGCCAG
CTTCGCCAGCCTGCTGGACGAGGCCGGCGTGGAAATCCTGCTGGTCGGCGACTCGCTGGGCCCGGTGATGCAGGGCGTGG
ACTCCACGCTGCCGGTCAGCGAAGAGGACATGCTCTACCACATCCGCTGCGTGGCCCGCGGCGCCAAGAACGCGCTGATC
CTCGGCGACATGACCTTCGGCGCCTACCAGGAGAGCCCGCAACAGGCCTTCGCCCATGCCGCGCGCCTGCTGCAGGCCGG
CGCCCACATGGTGAAGCTGGAAGGCGGCGCCTACATGGCCGAGACCACCCGCTTCCTGGTGGAGCGCGGCATTCCGGTGT
GCTCCCACATCGGCCTGACGCCGCAATACGTCAACATGTTCGGCGGCTACCGCGTGCAGGGCAGGGGCGAAGACGCGCAG
CGCATCCTGAACGACGCCAAGGTCCTGGCCGAGGCCGGCGCCAGCCTGGTGCTGATGGAGTGCGTGCCGGCGCCGCTGGC
CAAGGAGATCACCGAAACCGTCAAGGCGCCGACCATAGGCATAGGCGCGGGCGCGGACACGTCCGGCCAGGTGCTGGTGC
TGCACGACATGCTGGGCGTGTATCCGGGCAAGAAGGCCAAGTTCGTCAAGAACTTCATGGAAGAGGCCGGCAGCATCCAG
GGCGCTGTCCAGGCTTACATCAAGGCGGTGAAGGACAAGACCTTCCCGGCCGAAGAGCACACTTACTAA

Upstream 100 bases:

>100_bases
TGCATTGCCGAAATGCGCGGCCAGCGCAGTTATTTCAACAAGAGCGTCTGACCGGGTTGGTTCCCGCGCGGCGCCGAAGA
TGGAACGCGAGGAAGTCGAG

Downstream 100 bases:

>100_bases
GCCCGGAAGAGAACGAGAGATGGAAATCATCCGTACCGTGGCCGAGATGCGCGCCTGGCGCAAGCAGGCCGGCAAGCTGG
CTTTCGTGCCCACCATGGGC

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MKITVNTLHKLAEEGRKITMLTCYDASFASLLDEAGVEILLVGDSLGPVMQGVDSTLPVSEEDMLYHIRCVARGAKNALI
LGDMTFGAYQESPQQAFAHAARLLQAGAHMVKLEGGAYMAETTRFLVERGIPVCSHIGLTPQYVNMFGGYRVQGRGEDAQ
RILNDAKVLAEAGASLVLMECVPAPLAKEITETVKAPTIGIGAGADTSGQVLVLHDMLGVYPGKKAKFVKNFMEEAGSIQ
GAVQAYIKAVKDKTFPAEEHTY

Sequences:

>Translated_262_residues
MKITVNTLHKLAEEGRKITMLTCYDASFASLLDEAGVEILLVGDSLGPVMQGVDSTLPVSEEDMLYHIRCVARGAKNALI
LGDMTFGAYQESPQQAFAHAARLLQAGAHMVKLEGGAYMAETTRFLVERGIPVCSHIGLTPQYVNMFGGYRVQGRGEDAQ
RILNDAKVLAEAGASLVLMECVPAPLAKEITETVKAPTIGIGAGADTSGQVLVLHDMLGVYPGKKAKFVKNFMEEAGSIQ
GAVQAYIKAVKDKTFPAEEHTY
>Mature_262_residues
MKITVNTLHKLAEEGRKITMLTCYDASFASLLDEAGVEILLVGDSLGPVMQGVDSTLPVSEEDMLYHIRCVARGAKNALI
LGDMTFGAYQESPQQAFAHAARLLQAGAHMVKLEGGAYMAETTRFLVERGIPVCSHIGLTPQYVNMFGGYRVQGRGEDAQ
RILNDAKVLAEAGASLVLMECVPAPLAKEITETVKAPTIGIGAGADTSGQVLVLHDMLGVYPGKKAKFVKNFMEEAGSIQ
GAVQAYIKAVKDKTFPAEEHTY

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=260, Percent_Identity=50.7692307692308, Blast_Score=275, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6319653, Length=273, Percent_Identity=36.2637362637363, Blast_Score=169, Evalue=5e-43,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_CHRVO (Q7NXJ1)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901305.1
- ProteinModelPortal:   Q7NXJ1
- SMR:   Q7NXJ1
- GeneID:   2550478
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1635
- NMPDR:   fig|243365.1.peg.1635
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- PhylomeDB:   Q7NXJ1
- ProtClustDB:   PRK00311
- BioCyc:   CVIO243365:CV_1635-MONOMER
- BRENDA:   2.1.2.11
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28105; Mature: 28105

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: ACT_SITE 180-180 BINDING 83-83 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITVNTLHKLAEEGRKITMLTCYDASFASLLDEAGVEILLVGDSLGPVMQGVDSTLPVS
CEEEHHHHHHHHHCCCEEEEEEEECCHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCCCC
EEDMLYHIRCVARGAKNALILGDMTFGAYQESPQQAFAHAARLLQAGAHMVKLEGGAYMA
CCHHHHHHHHHHCCCCCEEEEECCCCCCHHCCHHHHHHHHHHHHHCCCEEEEECCCCHHH
ETTRFLVERGIPVCSHIGLTPQYVNMFGGYRVQGRGEDAQRILNDAKVLAEAGASLVLME
HHHHHHHHCCCCHHHHCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEEEH
CVPAPLAKEITETVKAPTIGIGAGADTSGQVLVLHDMLGVYPGKKAKFVKNFMEEAGSIQ
HCCCHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEHHHCCCCCCHHHHHHHHHHHHCCHH
GAVQAYIKAVKDKTFPAEEHTY
HHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKITVNTLHKLAEEGRKITMLTCYDASFASLLDEAGVEILLVGDSLGPVMQGVDSTLPVS
CEEEHHHHHHHHHCCCEEEEEEEECCHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCCCC
EEDMLYHIRCVARGAKNALILGDMTFGAYQESPQQAFAHAARLLQAGAHMVKLEGGAYMA
CCHHHHHHHHHHCCCCCEEEEECCCCCCHHCCHHHHHHHHHHHHHCCCEEEEECCCCHHH
ETTRFLVERGIPVCSHIGLTPQYVNMFGGYRVQGRGEDAQRILNDAKVLAEAGASLVLME
HHHHHHHHCCCCHHHHCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEEEH
CVPAPLAKEITETVKAPTIGIGAGADTSGQVLVLHDMLGVYPGKKAKFVKNFMEEAGSIQ
HCCCHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEHHHCCCCCCHHHHHHHHHHHHCCHH
GAVQAYIKAVKDKTFPAEEHTY
HHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782