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The map label for this gene is pcnB [H]

Identifier: 34497087

GI number: 34497087

Start: 1770252

End: 1771604

Strand: Direct

Name: pcnB [H]

Synonym: CV_1632

Alternate gene names: 34497087

Gene position: 1770252-1771604 (Clockwise)

Preceding gene: 34497086

Following gene: 34497088

Centisome position: 37.26

GC content: 68.59

Gene sequence:

>1353_bases
ATGATCCGCAAGCTCATCCGTAAAGTCCTGGAATTGCCCATGGGCAAACGCCGCGGCAAACCGCGCGTCTATCCGTTGCC
GCAGCACGGCATCCGCCGCGACCAGCTCAGCCACGCCGCGCTGCGCGTGACTTCCCGGCTGCAGGAAGCCGGCTTCTCCG
CCTTCGTGGTGGGCGGCGCCGTGCGCGACCTGATGCTGGGCGTGTCGCCCAAGGATTTCGACGTGGCCACCAACGCCACG
CCGGAGCAGGTGCACCACCTGTTCCGCCGCTCCCGCATCATCGGCCGCCGCTTCCGCATCGTCCACGTGATGATGGGCCC
GGAAACCATAGAAGTGACCACCTTCCGCGGCGGCAGCGTCGACGACACCAATGAAACCGGCCGCATCATGGCCGACAACA
GCTTCGGCAGCCAGGAAGAGGACGCGCACCGGCGCGACTTCACCGTCAACGCGCTGTTCTACGACCCGTCCGACGAAACC
GTCATCGACTACCACCACGGCGTCAAGGACCTGCACGCGAAGAAACTGGTGATGATAGGCCAGCCGGCACGCCGCTATCA
GGAAGACCCGGTGCGCATGCTGCGCGCGGTGCGCCTGGCGGCCAAGCTGGGCTTCGAGATCGACGAGGACACCAAAAAGC
CGATCCGCGCGCATGCCCACCTGCTGAAGAAAGAGCCGCCGGCCCGTTTGTTCGACGAGCTGCTGAAGCTGCTGATGTCC
GGCCACGCCTACGCCTGCCTGAAAAAGCTGCGCGAGGAGGGCCTGGCGCGCGGCGTGTTCCCGCTGCTGGACGCGGTGAT
CGACGACAAGGGCGGCGAGGACGCCTTCCTGAAGCTGAGCCTGGACAACACCGACGCCCGGCTGCGCGCCGACAAGCCGA
TTTCCGTCGGCTTCCTGCTGGCCACGCTGCTGTGGCGCCAGGTCGACCAGGGCTGGCAGCGCCGCCGCGAGGCGGGCGAG
AAGAGCCTGCCGGCGCTGCTGGACGCCATTTCCGACGTCGAGAACGAACAGGACAACGATTTCGCCATTCCGCGCCGCTT
CAGCGTGACCATGCGCGAGATCTGGACGCTGCAGGCGCGCTTCGACAGCCGCACCGGCGCCCGCCCATACCGCTTCCTTG
AGCAGCCCCGCTTCCGCGCCGCCTATGATTTCCTGGCGCTGCGCAGCGAGGCCGGCGAGGTGCCGCGCGCGCTGGTGGAG
TGGTGGGAGCGCTTCCAGCGCGCCGACGCCGACGAGTGCGAGCGCCTGATCGCCGAAGCCAAGGAAGGCGGCGCCGAGGA
GCCCGCCAAGAAGCGCCGCCGCCGGCGCAGCGGCCGCCGCAAGCCCGAGGGCGCGGGGGAGAGCGCCGAGTGA

Upstream 100 bases:

>100_bases
GGGAAGGGCCGATGTGTATTTTCCGCGGGGATTCGGGCTATAATCGCGGCCTTGAATCCCTAACCTTGCGAATCCAGCAT
TCCAAGCCACTATCTTCCAC

Downstream 100 bases:

>100_bases
CGCTGGCCTACGTGGCTCTGGGCAGCAATCTGGAGCAGCCTCAGCAGCAAGTCAGGGCCGCTCTTGCGGCCCTTGGCCAT
TTGGATGGCGCCACGTTGCT

Product: polynucleotide adenylyltransferase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MIRKLIRKVLELPMGKRRGKPRVYPLPQHGIRRDQLSHAALRVTSRLQEAGFSAFVVGGAVRDLMLGVSPKDFDVATNAT
PEQVHHLFRRSRIIGRRFRIVHVMMGPETIEVTTFRGGSVDDTNETGRIMADNSFGSQEEDAHRRDFTVNALFYDPSDET
VIDYHHGVKDLHAKKLVMIGQPARRYQEDPVRMLRAVRLAAKLGFEIDEDTKKPIRAHAHLLKKEPPARLFDELLKLLMS
GHAYACLKKLREEGLARGVFPLLDAVIDDKGGEDAFLKLSLDNTDARLRADKPISVGFLLATLLWRQVDQGWQRRREAGE
KSLPALLDAISDVENEQDNDFAIPRRFSVTMREIWTLQARFDSRTGARPYRFLEQPRFRAAYDFLALRSEAGEVPRALVE
WWERFQRADADECERLIAEAKEGGAEEPAKKRRRRRSGRRKPEGAGESAE

Sequences:

>Translated_450_residues
MIRKLIRKVLELPMGKRRGKPRVYPLPQHGIRRDQLSHAALRVTSRLQEAGFSAFVVGGAVRDLMLGVSPKDFDVATNAT
PEQVHHLFRRSRIIGRRFRIVHVMMGPETIEVTTFRGGSVDDTNETGRIMADNSFGSQEEDAHRRDFTVNALFYDPSDET
VIDYHHGVKDLHAKKLVMIGQPARRYQEDPVRMLRAVRLAAKLGFEIDEDTKKPIRAHAHLLKKEPPARLFDELLKLLMS
GHAYACLKKLREEGLARGVFPLLDAVIDDKGGEDAFLKLSLDNTDARLRADKPISVGFLLATLLWRQVDQGWQRRREAGE
KSLPALLDAISDVENEQDNDFAIPRRFSVTMREIWTLQARFDSRTGARPYRFLEQPRFRAAYDFLALRSEAGEVPRALVE
WWERFQRADADECERLIAEAKEGGAEEPAKKRRRRRSGRRKPEGAGESAE
>Mature_450_residues
MIRKLIRKVLELPMGKRRGKPRVYPLPQHGIRRDQLSHAALRVTSRLQEAGFSAFVVGGAVRDLMLGVSPKDFDVATNAT
PEQVHHLFRRSRIIGRRFRIVHVMMGPETIEVTTFRGGSVDDTNETGRIMADNSFGSQEEDAHRRDFTVNALFYDPSDET
VIDYHHGVKDLHAKKLVMIGQPARRYQEDPVRMLRAVRLAAKLGFEIDEDTKKPIRAHAHLLKKEPPARLFDELLKLLMS
GHAYACLKKLREEGLARGVFPLLDAVIDDKGGEDAFLKLSLDNTDARLRADKPISVGFLLATLLWRQVDQGWQRRREAGE
KSLPALLDAISDVENEQDNDFAIPRRFSVTMREIWTLQARFDSRTGARPYRFLEQPRFRAAYDFLALRSEAGEVPRALVE
WWERFQRADADECERLIAEAKEGGAEEPAKKRRRRRSGRRKPEGAGESAE

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=197, Percent_Identity=35.5329949238579, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI87081691, Length=440, Percent_Identity=45.2272727272727, Blast_Score=347, Evalue=6e-97,
Organism=Escherichia coli, GI1789436, Length=220, Percent_Identity=34.0909090909091, Blast_Score=85, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71995920, Length=206, Percent_Identity=31.0679611650485, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI281360112, Length=192, Percent_Identity=33.8541666666667, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21357337, Length=192, Percent_Identity=33.8541666666667, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 51389; Mature: 51389

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKLIRKVLELPMGKRRGKPRVYPLPQHGIRRDQLSHAALRVTSRLQEAGFSAFVVGGA
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHH
VRDLMLGVSPKDFDVATNATPEQVHHLFRRSRIIGRRFRIVHVMMGPETIEVTTFRGGSV
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCEEEEEEECCCCC
DDTNETGRIMADNSFGSQEEDAHRRDFTVNALFYDPSDETVIDYHHGVKDLHAKKLVMIG
CCCCCCCCEEECCCCCCCCHHHHHCCEEEEEEEECCCCCEEEEHHCCHHHHHHHHEEEEC
QPARRYQEDPVRMLRAVRLAAKLGFEIDEDTKKPIRAHAHLLKKEPPARLFDELLKLLMS
CCHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
GHAYACLKKLREEGLARGVFPLLDAVIDDKGGEDAFLKLSLDNTDARLRADKPISVGFLL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEECCCCCHHHHHH
ATLLWRQVDQGWQRRREAGEKSLPALLDAISDVENEQDNDFAIPRRFSVTMREIWTLQAR
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
FDSRTGARPYRFLEQPRFRAAYDFLALRSEAGEVPRALVEWWERFQRADADECERLIAEA
HCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
KEGGAEEPAKKRRRRRSGRRKPEGAGESAE
HCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MIRKLIRKVLELPMGKRRGKPRVYPLPQHGIRRDQLSHAALRVTSRLQEAGFSAFVVGGA
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHH
VRDLMLGVSPKDFDVATNATPEQVHHLFRRSRIIGRRFRIVHVMMGPETIEVTTFRGGSV
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCEEEEEEECCCCC
DDTNETGRIMADNSFGSQEEDAHRRDFTVNALFYDPSDETVIDYHHGVKDLHAKKLVMIG
CCCCCCCCEEECCCCCCCCHHHHHCCEEEEEEEECCCCCEEEEHHCCHHHHHHHHEEEEC
QPARRYQEDPVRMLRAVRLAAKLGFEIDEDTKKPIRAHAHLLKKEPPARLFDELLKLLMS
CCHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
GHAYACLKKLREEGLARGVFPLLDAVIDDKGGEDAFLKLSLDNTDARLRADKPISVGFLL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEECCCCCHHHHHH
ATLLWRQVDQGWQRRREAGEKSLPALLDAISDVENEQDNDFAIPRRFSVTMREIWTLQAR
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
FDSRTGARPYRFLEQPRFRAAYDFLALRSEAGEVPRALVEWWERFQRADADECERLIAEA
HCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
KEGGAEEPAKKRRRRRSGRRKPEGAGESAE
HCCCCCCHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]