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The map label for this gene is cobA1 [H]

Identifier: 34497024

GI number: 34497024

Start: 1682198

End: 1683034

Strand: Reverse

Name: cobA1 [H]

Synonym: CV_1569

Alternate gene names: 34497024

Gene position: 1683034-1682198 (Counterclockwise)

Preceding gene: 34497025

Following gene: 34497023

Centisome position: 35.42

GC content: 68.94

Gene sequence:

>837_bases
ATGACGGGCAAGGTTTACCTGATAGGCGCCGGCCCCGGCGACCTGGACATGCTGACGCTGAAGGCCGCGCGCTGCCTGCA
GCTGGCCGACGTGGCGCTGGTGGACGATCTGGTCCATCCGGACATTCGCGCCATGCTGCGGCCGGACGCCGAAATCGTGC
CGGTCGGCAAGCGCGGCGGCTGCCCGTCGGCGTCGCAAGCCGCCATCGAACAGCGGATGGTCGACGAAGCCCACGCCGGC
AAAACGGTGGCGCGCCTCAAGGGCGGCGATCCCTTCGTCTTCGGCCGCGGCGGCGAGGAAATGCTGACGCTGCAAGCGGC
CGGCGTCGAGGTGGAGATCGTCAACGGCCTTAGCGCCGGCCTGGCGGTGCCGGCCACGCTGGGCATTCCGTTGACCCACC
GCAGCTTCGTCCACGGCGTGACCTTCGTCTCCGGCCATCCGCATCAGGACGGCGACGAGCCCAACTGGCAGGCGCTGGCG
CAAAGCGGCATGACGCTGGTGATCTATATGGGCGTCAAACGGCTGCCGCTGATCCGCGCCGAGCTGCTGCGCCACGGCCT
GAAAGCCGACACCCCGGCGGCGGCGATAGAAAACGGCACGCTGCCGCAGCAGCGCCAGGTGCTGAGCACCCTGGACGCTT
TGGAACAAGACATGGGCGCGGCCGGCATCCACAGCCCGGCGCTGATCGTCATCGGACCGACGGTGGCGCTGGCGTCCTGT
AGCCCCTCAGCACTCGAGCGCAGCGAGGCTCCCTTGCAGGGCAAGGGCGGCATGCGTGCGAGCCTTGAGGCTCGCTCAGC
GGAAGGATGGGAGCGGATCAAGATGGCTGCCGACTGA

Upstream 100 bases:

>100_bases
CGCGAGCTGGCCGAGGCCCTGGGCGCGGAATACCTGGCTTGCGACGCCCTCACCGGCGAGCAGCTGACATTGACGATTCA
ACAAAGACTGGGCCGCTGAA

Downstream 100 bases:

>100_bases
ACTCGCACAATGAAGAGGCCCGGCTTTGCCGGGTCCTGTAGAACAGAATCGAATGACTACATTGCAGCCAAAGGAATACG
CATGATCATCTGCATAGGCG

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MTGKVYLIGAGPGDLDMLTLKAARCLQLADVALVDDLVHPDIRAMLRPDAEIVPVGKRGGCPSASQAAIEQRMVDEAHAG
KTVARLKGGDPFVFGRGGEEMLTLQAAGVEVEIVNGLSAGLAVPATLGIPLTHRSFVHGVTFVSGHPHQDGDEPNWQALA
QSGMTLVIYMGVKRLPLIRAELLRHGLKADTPAAAIENGTLPQQRQVLSTLDALEQDMGAAGIHSPALIVIGPTVALASC
SPSALERSEAPLQGKGGMRASLEARSAEGWERIKMAAD

Sequences:

>Translated_278_residues
MTGKVYLIGAGPGDLDMLTLKAARCLQLADVALVDDLVHPDIRAMLRPDAEIVPVGKRGGCPSASQAAIEQRMVDEAHAG
KTVARLKGGDPFVFGRGGEEMLTLQAAGVEVEIVNGLSAGLAVPATLGIPLTHRSFVHGVTFVSGHPHQDGDEPNWQALA
QSGMTLVIYMGVKRLPLIRAELLRHGLKADTPAAAIENGTLPQQRQVLSTLDALEQDMGAAGIHSPALIVIGPTVALASC
SPSALERSEAPLQGKGGMRASLEARSAEGWERIKMAAD
>Mature_277_residues
TGKVYLIGAGPGDLDMLTLKAARCLQLADVALVDDLVHPDIRAMLRPDAEIVPVGKRGGCPSASQAAIEQRMVDEAHAGK
TVARLKGGDPFVFGRGGEEMLTLQAAGVEVEIVNGLSAGLAVPATLGIPLTHRSFVHGVTFVSGHPHQDGDEPNWQALAQ
SGMTLVIYMGVKRLPLIRAELLRHGLKADTPAAAIENGTLPQQRQVLSTLDALEQDMGAAGIHSPALIVIGPTVALASCS
PSALERSEAPLQGKGGMRASLEARSAEGWERIKMAAD

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=235, Percent_Identity=45.531914893617, Blast_Score=192, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6322922, Length=233, Percent_Identity=32.1888412017167, Blast_Score=112, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 29076; Mature: 28945

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGKVYLIGAGPGDLDMLTLKAARCLQLADVALVDDLVHPDIRAMLRPDAEIVPVGKRGG
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEECCCCCC
CPSASQAAIEQRMVDEAHAGKTVARLKGGDPFVFGRGGEEMLTLQAAGVEVEIVNGLSAG
CCCHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCEEEEEEECCEEEEEECCCCCC
LAVPATLGIPLTHRSFVHGVTFVSGHPHQDGDEPNWQALAQSGMTLVIYMGVKRLPLIRA
CCCCEECCCCCCHHHHHHEEEEECCCCCCCCCCCCHHHHHHCCCEEEEEECCCCCHHHHH
ELLRHGLKADTPAAAIENGTLPQQRQVLSTLDALEQDMGAAGIHSPALIVIGPTVALASC
HHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEECC
SPSALERSEAPLQGKGGMRASLEARSAEGWERIKMAAD
CHHHHHHCCCCCCCCCCCEEEECCCCCCCHHHHEECCC
>Mature Secondary Structure 
TGKVYLIGAGPGDLDMLTLKAARCLQLADVALVDDLVHPDIRAMLRPDAEIVPVGKRGG
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEECCCCCC
CPSASQAAIEQRMVDEAHAGKTVARLKGGDPFVFGRGGEEMLTLQAAGVEVEIVNGLSAG
CCCHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCEEEEEEECCEEEEEECCCCCC
LAVPATLGIPLTHRSFVHGVTFVSGHPHQDGDEPNWQALAQSGMTLVIYMGVKRLPLIRA
CCCCEECCCCCCHHHHHHEEEEECCCCCCCCCCCCHHHHHHCCCEEEEEECCCCCHHHHH
ELLRHGLKADTPAAAIENGTLPQQRQVLSTLDALEQDMGAAGIHSPALIVIGPTVALASC
HHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEECC
SPSALERSEAPLQGKGGMRASLEARSAEGWERIKMAAD
CHHHHHHCCCCCCCCCCCEEEECCCCCCCHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]