Click here to switch to the map view.

The map label for this gene is cof [H]

Identifier: 34496981

GI number: 34496981

Start: 1633070

End: 1633879

Strand: Direct

Name: cof [H]

Synonym: CV_1526

Alternate gene names: 34496981

Gene position: 1633070-1633879 (Clockwise)

Preceding gene: 34496978

Following gene: 34496983

Centisome position: 34.37

GC content: 66.05

Gene sequence:

>810_bases
TTGCACCGATGCGAACACGGAGATTCTGACATGCGACTGATTGTTTCCGATCTGGACGGCACCTTGCTGGACAAGGACGC
GCGGCTGGCCGACGAGACGCGCGCCGCTTTGCAGGCGGCGGTGGCGCGCGGCGTGGCCCTGGCGGTGGCCACCGGCCGGC
ATGAGCGCCAGGTGAGGAAGCTGTGGCCGCGGGATCTGCCTCCGGTGCCGGTGATCAGCGCCAACGGCGCGCGCATCCAC
CTGGCGGACGGCACGCTGTTGCACCAGAGCCGCCTGTCGCCGGCCTTGCTGGCGAGGCTGCTACGGCCGGAATTGATCCG
GGAGACTGAACTGGGCGTCTACCGCGACGATTGCATCATGGCCTACCACAGCCGGCGCGAATACAGCCATTACACCGGCG
AAGTGACGGAAATCGAGGATCTGGCGGGTTTCAAGGCCGAAGATGTGTCCAAGGTGATCTTCTGCGGCACGCCGGAGCAA
CTGAAAGCGGTAGAGGCCGACATCGCCCGAGAACTGGGCGACGAAGTGTCCATGACCTATTCCCACATTTGTTACCTGGA
AGTGATGGCGCCCGGCGTGCACAAGGGTAGCGCGCTGCGCCGCCTGCTCGATCACCTGGACATCGCGGCGGAAGATTGCG
CGGCCTTCGGCGACAACCTGAACGATGCCGAGATGCTGGCCTTGGCGGGCCTGCCGCACGTGATGGGCAACGCGCATCCG
GAATTGAAGCGCCGCCTGCCCGGCGCGCCCGTGATAGGCGACCACGACCAGGCTGGCGTGGCGCGGCAGTTGCGGGCTTT
GCTGGGCTGA

Upstream 100 bases:

>100_bases
CGGCATTTTTGCACGATCCCGCAAGCCAGGTCAAAGATTCGGCATTTTTTGTGCAAAAACATGCATGTTTGTTTGCGTGT
TTTTGCACGATCGTGCATTA

Downstream 100 bases:

>100_bases
GAGCGCTGACAGAGCCTCGCCGAGCGCCTGGGCCAAGGCGCCCCGACGCAGACAGCACAAGCAGTACGACAAGGAGACGC
AGCGCAGGATCAGGGGTTTA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MHRCEHGDSDMRLIVSDLDGTLLDKDARLADETRAALQAAVARGVALAVATGRHERQVRKLWPRDLPPVPVISANGARIH
LADGTLLHQSRLSPALLARLLRPELIRETELGVYRDDCIMAYHSRREYSHYTGEVTEIEDLAGFKAEDVSKVIFCGTPEQ
LKAVEADIARELGDEVSMTYSHICYLEVMAPGVHKGSALRRLLDHLDIAAEDCAAFGDNLNDAEMLALAGLPHVMGNAHP
ELKRRLPGAPVIGDHDQAGVARQLRALLG

Sequences:

>Translated_269_residues
MHRCEHGDSDMRLIVSDLDGTLLDKDARLADETRAALQAAVARGVALAVATGRHERQVRKLWPRDLPPVPVISANGARIH
LADGTLLHQSRLSPALLARLLRPELIRETELGVYRDDCIMAYHSRREYSHYTGEVTEIEDLAGFKAEDVSKVIFCGTPEQ
LKAVEADIARELGDEVSMTYSHICYLEVMAPGVHKGSALRRLLDHLDIAAEDCAAFGDNLNDAEMLALAGLPHVMGNAHP
ELKRRLPGAPVIGDHDQAGVARQLRALLG
>Mature_269_residues
MHRCEHGDSDMRLIVSDLDGTLLDKDARLADETRAALQAAVARGVALAVATGRHERQVRKLWPRDLPPVPVISANGARIH
LADGTLLHQSRLSPALLARLLRPELIRETELGVYRDDCIMAYHSRREYSHYTGEVTEIEDLAGFKAEDVSKVIFCGTPEQ
LKAVEADIARELGDEVSMTYSHICYLEVMAPGVHKGSALRRLLDHLDIAAEDCAAFGDNLNDAEMLALAGLPHVMGNAHP
ELKRRLPGAPVIGDHDQAGVARQLRALLG

Specific function: Catalyzes the hydrolysis of 4-amino-2-methyl-5- hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2- methyl-5-hydroxymethylpyrimidine phosphate (HMP-P) [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI48994981, Length=265, Percent_Identity=36.9811320754717, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI87081741, Length=263, Percent_Identity=31.9391634980989, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1787043, Length=247, Percent_Identity=25.9109311740891, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI2367265, Length=246, Percent_Identity=30.8943089430894, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI87081790, Length=244, Percent_Identity=26.2295081967213, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF08282 Hydrolase_3 [H]

EC number: NA

Molecular weight: Translated: 29463; Mature: 29463

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRCEHGDSDMRLIVSDLDGTLLDKDARLADETRAALQAAVARGVALAVATGRHERQVRK
CCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHH
LWPRDLPPVPVISANGARIHLADGTLLHQSRLSPALLARLLRPELIRETELGVYRDDCIM
HCCCCCCCCCEEECCCCEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
AYHSRREYSHYTGEVTEIEDLAGFKAEDVSKVIFCGTPEQLKAVEADIARELGDEVSMTY
HHHHHHHHHHHCCCHHHHHHHCCCCHHCCCEEEEECCHHHHHHHHHHHHHHHCHHHHHHH
SHICYLEVMAPGVHKGSALRRLLDHLDIAAEDCAAFGDNLNDAEMLALAGLPHVMGNAHP
HHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHCCCCH
ELKRRLPGAPVIGDHDQAGVARQLRALLG
HHHHHCCCCCCCCCCCHHHHHHHHHHHHC
>Mature Secondary Structure
MHRCEHGDSDMRLIVSDLDGTLLDKDARLADETRAALQAAVARGVALAVATGRHERQVRK
CCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHH
LWPRDLPPVPVISANGARIHLADGTLLHQSRLSPALLARLLRPELIRETELGVYRDDCIM
HCCCCCCCCCEEECCCCEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
AYHSRREYSHYTGEVTEIEDLAGFKAEDVSKVIFCGTPEQLKAVEADIARELGDEVSMTY
HHHHHHHHHHHCCCHHHHHHHCCCCHHCCCEEEEECCHHHHHHHHHHHHHHHCHHHHHHH
SHICYLEVMAPGVHKGSALRRLLDHLDIAAEDCAAFGDNLNDAEMLALAGLPHVMGNAHP
HHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHCCCCH
ELKRRLPGAPVIGDHDQAGVARQLRALLG
HHHHHCCCCCCCCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA