Click here to switch to the map view.

The map label for this gene is pilJ [H]

Identifier: 34496979

GI number: 34496979

Start: 1630527

End: 1631642

Strand: Reverse

Name: pilJ [H]

Synonym: CV_1524

Alternate gene names: 34496979

Gene position: 1631642-1630527 (Counterclockwise)

Preceding gene: 34496980

Following gene: 34496975

Centisome position: 34.34

GC content: 70.16

Gene sequence:

>1116_bases
CTGGCGATGTCGCTGGCCGCCGTCGGCATCGTCATGTGGCTGTCCGGCATCGACGCGCTGCCCAAGAGCCCGCTGCCGCG
CGAGGCGCTGCCCAAGCTGCAGGCCAAGTCGCTGGCCGGCCTGTCGCTGGTGGTGCTGGTGCTGGCGCTGGCCTTCGACA
AGGCCAAGAGCAAGAGCTTCGCTAAGCTGGAAACCGCGCGCGCCGAGGCCGAGCGCGCCGGCGAGGCGCTGGCGCGGATG
ATGGAGCAGATCGGCCATTCGATCCGCGACGCCTCCGGCGCCAGCCGCCAGATCGCCGACAGCACCGGCAGCATGGCGCG
CACCATGGCCGAGCAGCGCGAACGCTCCGAGGACATGGTGGTGGTGGCGCAGCAGATGGCGGTGGTCACCAGCCAGAACG
CCGAGCAATCGACCGCCGCCACGCTGCTGGCGCAGAATTCCGGCGAAGCCGCGCGCGAAGGCGGCAAGGCGATGAACCAA
GCGGTGGAACAGCTGGGCCGCGCCGGCGAAGTGATAGGCCACGCCTCGAACCGGCTGGAGGACCTGGGCCAGCGCAGCGC
CGAGGTCAGCGGCATCGTGCAGCTGATACGCGACATCGCCGACCAGACCAATCTGTTGGCCTTGAACGCCGCGATCGAAG
CCGCGCGCGCCGGCGAAACCGGCCGCGGCTTCGCGGTGGTGGCCGACGAGGTGAGAAAACTGGCCGAGCGCACCCAGAAC
GCCACCAAGGACATCGAGAGCAAGATCCGGGTGATCGTCGACGGCACCAACCAGGCGCTGGTGGCGATGCGCGACGGCAA
CCAGCAGATGCGCGCCGGCCGCGACCACGCGCAGGAAGCGCAGCGCAAGCTGGCCGGAATCATTGACGGCGCCGGCGAGC
TGGCCGGCCTGCTGCAGCAGGTGTCCCAGGCCGAGGCCAGCCAGAACCAGGGCTTCTCCCAGTTCGCCGGCGACATCGTC
GCCGTCGGCGAATCCAGCCGCAGCCTGTCCGGCGAAACCCACAACATCGCCGAGGCGGTGCGCCGGCTGGACGAGCAGAT
GGACAAGCTGCACCAGGCGATAAGCCGCCAGGAGACGCCCTCGGCCGATGCCAGGCGCGAACCCGCCTGGCGCTGA

Upstream 100 bases:

>100_bases
GTTCGTCAACGGCGGCATCCTGTCCACCAGCCTGATGTGGTTCGCCGCCATTCCCTTCACCGCCGTCTTCATCGCCACCC
GCCGCTCCGGCATGTTCTGG

Downstream 100 bases:

>100_bases
GGCGGGCTCGGCTCAGATGTAGCCGAGCTCCAGCCCGCGCAGCACCGCGCGCACCCGGTCGCGCACGCCGAGCTTGGACA
GGATGCTGGAGACGTGATTC

Product: methyl-accepting chemotaxis protein II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MAMSLAAVGIVMWLSGIDALPKSPLPREALPKLQAKSLAGLSLVVLVLALAFDKAKSKSFAKLETARAEAERAGEALARM
MEQIGHSIRDASGASRQIADSTGSMARTMAEQRERSEDMVVVAQQMAVVTSQNAEQSTAATLLAQNSGEAAREGGKAMNQ
AVEQLGRAGEVIGHASNRLEDLGQRSAEVSGIVQLIRDIADQTNLLALNAAIEAARAGETGRGFAVVADEVRKLAERTQN
ATKDIESKIRVIVDGTNQALVAMRDGNQQMRAGRDHAQEAQRKLAGIIDGAGELAGLLQQVSQAEASQNQGFSQFAGDIV
AVGESSRSLSGETHNIAEAVRRLDEQMDKLHQAISRQETPSADARREPAWR

Sequences:

>Translated_371_residues
MAMSLAAVGIVMWLSGIDALPKSPLPREALPKLQAKSLAGLSLVVLVLALAFDKAKSKSFAKLETARAEAERAGEALARM
MEQIGHSIRDASGASRQIADSTGSMARTMAEQRERSEDMVVVAQQMAVVTSQNAEQSTAATLLAQNSGEAAREGGKAMNQ
AVEQLGRAGEVIGHASNRLEDLGQRSAEVSGIVQLIRDIADQTNLLALNAAIEAARAGETGRGFAVVADEVRKLAERTQN
ATKDIESKIRVIVDGTNQALVAMRDGNQQMRAGRDHAQEAQRKLAGIIDGAGELAGLLQQVSQAEASQNQGFSQFAGDIV
AVGESSRSLSGETHNIAEAVRRLDEQMDKLHQAISRQETPSADARREPAWR
>Mature_370_residues
AMSLAAVGIVMWLSGIDALPKSPLPREALPKLQAKSLAGLSLVVLVLALAFDKAKSKSFAKLETARAEAERAGEALARMM
EQIGHSIRDASGASRQIADSTGSMARTMAEQRERSEDMVVVAQQMAVVTSQNAEQSTAATLLAQNSGEAAREGGKAMNQA
VEQLGRAGEVIGHASNRLEDLGQRSAEVSGIVQLIRDIADQTNLLALNAAIEAARAGETGRGFAVVADEVRKLAERTQNA
TKDIESKIRVIVDGTNQALVAMRDGNQQMRAGRDHAQEAQRKLAGIIDGAGELAGLLQQVSQAEASQNQGFSQFAGDIVA
VGESSRSLSGETHNIAEAVRRLDEQMDKLHQAISRQETPSADARREPAWR

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1789453, Length=235, Percent_Identity=32.7659574468085, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787690, Length=289, Percent_Identity=29.4117647058824, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI2367378, Length=236, Percent_Identity=31.7796610169492, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1788195, Length=252, Percent_Identity=29.3650793650794, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788194, Length=126, Percent_Identity=39.6825396825397, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 39449; Mature: 39318

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMSLAAVGIVMWLSGIDALPKSPLPREALPKLQAKSLAGLSLVVLVLALAFDKAKSKSF
CCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
AKLETARAEAERAGEALARMMEQIGHSIRDASGASRQIADSTGSMARTMAEQRERSEDMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
VVAQQMAVVTSQNAEQSTAATLLAQNSGEAAREGGKAMNQAVEQLGRAGEVIGHASNRLE
HHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLGQRSAEVSGIVQLIRDIADQTNLLALNAAIEAARAGETGRGFAVVADEVRKLAERTQN
HHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
ATKDIESKIRVIVDGTNQALVAMRDGNQQMRAGRDHAQEAQRKLAGIIDGAGELAGLLQQ
HHHHHHHHEEEEEECCCCEEEEEECCCHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHH
VSQAEASQNQGFSQFAGDIVAVGESSRSLSGETHNIAEAVRRLDEQMDKLHQAISRQETP
HHHHHHHHCCCHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SADARREPAWR
CCCCCCCCCCC
>Mature Secondary Structure 
AMSLAAVGIVMWLSGIDALPKSPLPREALPKLQAKSLAGLSLVVLVLALAFDKAKSKSF
CHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
AKLETARAEAERAGEALARMMEQIGHSIRDASGASRQIADSTGSMARTMAEQRERSEDMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
VVAQQMAVVTSQNAEQSTAATLLAQNSGEAAREGGKAMNQAVEQLGRAGEVIGHASNRLE
HHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLGQRSAEVSGIVQLIRDIADQTNLLALNAAIEAARAGETGRGFAVVADEVRKLAERTQN
HHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
ATKDIESKIRVIVDGTNQALVAMRDGNQQMRAGRDHAQEAQRKLAGIIDGAGELAGLLQQ
HHHHHHHHEEEEEECCCCEEEEEECCCHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHH
VSQAEASQNQGFSQFAGDIVAVGESSRSLSGETHNIAEAVRRLDEQMDKLHQAISRQETP
HHHHHHHHCCCHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SADARREPAWR
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]