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The map label for this gene is rimO

Identifier: 34496828

GI number: 34496828

Start: 1445751

End: 1447067

Strand: Direct

Name: rimO

Synonym: CV_1373

Alternate gene names: 34496828

Gene position: 1445751-1447067 (Clockwise)

Preceding gene: 34496827

Following gene: 34496829

Centisome position: 30.43

GC content: 65.0

Gene sequence:

>1317_bases
ATGAACAAGACTCCCCGCGTCGGATTCGTTTCGCTTGGCTGTCCCAAGGCCGCCAGCGATTCCGAGCAGATCCTGACCCG
CCTTCGCGCCGAAGGCTATGAAATCGCGCCCTCCTACGATGGCGCCGACCTGGTGGTGGTGAACACCTGCGGCTTCATCG
ATTCCGCCGTCGAGGAGTCGCTGGACGCCATCGGCGAAGCGCTGAACGAGAACGGCAAAGTGATCGTCACCGGCTGTCTG
GGGGCCAAGGGCGACGTGGTGCGCGACGTGCATCCGTCGGTCTTGGCCGTGACCGGACCGCATGCCACCGAGGAAGTGAT
GAGCGCGGTGCATACCCACCTGCCCAAGCCGCACGACCCCTTCGTCGACCTGGTGCCGGACATCGGCGTGCGCCTGACGC
CCAAGCATTACGCCTACCTGAAGATTTCCGAGGGCTGCAACCACCGCTGCACCTTCTGCATCATCCCGTCGATGCGCGGC
GATCTGGAAAGCCGCCCCATCCATGACGTGCTGCGCGAGGCCGAAAGCCTGGCCAAGGCCGGCGTGAAAGAGATCCTGGT
GATTTCGCAGGACACCTCGGCCTACGGCGTGGACACCAAGTACAAGCTGGGCTTCCACAACGGCCGCCCGGTGAAGACCC
GCATGACCGAGCTGTGCGAGGAGCTGGGCCGCCACGGCATCTGGGTGCGCCTGCATTACGTTTACCCGTACCCGCACGTG
GACGAGGTGATTCCGCTGATGCGCGACGGCAAGATCCTGCCGTATCTCGACATCCCGTTCCAGCACGCCAGCCAGAAAGT
GCTGAAGCTGATGAAGCGGCCGGCCAACAGCGACAACGTGCTGGCCCGCATCAAGAAGTGGCGCGAGATCTGCCCGGAAC
TGGTGATCCGCTCCACCTTCATCGTCGGCTTCCCCGGCGAGACCGAGGAAGACTTCGAAGAATTGCTGGCGTTCATCCGC
GAAGCGGAGCTGGACCGCGTCGGCTGCTTCACTTACTCCCCGGTCGAAGGCGCCACCGCCAACGAGTTGCCGAACCCGGT
GCCGGAAGACGTGAAGGAAGCGCGCAAGGAGCGCTTCATGGCCGTGCAGGCCGAAATCAGCGCGCGCCGCCTGGAGCGCC
GCGTCGGCCAGACCCTGCAAGTGCTGGTGGACGAGATCGACGACGAAGGCACCGCCGTTTGCCGCAGCTATGCCGACGCG
CCGGAGATCGACGGCCTGGTGTTCGTCGAGGACGCCGCCGGCATGCAGCCGGGCGAGTTCTACCAGGTGGAAATCGTCGA
CTGCAGCGAGCACGACCTGTGGGGCGAGCGCCGCTGA

Upstream 100 bases:

>100_bases
GCGGGCGACGCCTGATCGATGCGCGCCGAAGACAGATGCGTATCGCGAGGCAAACCGTTGTATAATCGCGCGATTTTGTT
TTCGCCCCAAGATGTTGAAC

Downstream 100 bases:

>100_bases
GGCGCGGCTCGCTGGCAGGCCAAGGCGCCGGGTTTCGACCCGGCGCTTTTTTTTATTTCGGATGCGGTAGATTTTTTGAC
ATTCCAGCCCTTGTCGCGGA

Product: oxidoreductase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MNKTPRVGFVSLGCPKAASDSEQILTRLRAEGYEIAPSYDGADLVVVNTCGFIDSAVEESLDAIGEALNENGKVIVTGCL
GAKGDVVRDVHPSVLAVTGPHATEEVMSAVHTHLPKPHDPFVDLVPDIGVRLTPKHYAYLKISEGCNHRCTFCIIPSMRG
DLESRPIHDVLREAESLAKAGVKEILVISQDTSAYGVDTKYKLGFHNGRPVKTRMTELCEELGRHGIWVRLHYVYPYPHV
DEVIPLMRDGKILPYLDIPFQHASQKVLKLMKRPANSDNVLARIKKWREICPELVIRSTFIVGFPGETEEDFEELLAFIR
EAELDRVGCFTYSPVEGATANELPNPVPEDVKEARKERFMAVQAEISARRLERRVGQTLQVLVDEIDDEGTAVCRSYADA
PEIDGLVFVEDAAGMQPGEFYQVEIVDCSEHDLWGERR

Sequences:

>Translated_438_residues
MNKTPRVGFVSLGCPKAASDSEQILTRLRAEGYEIAPSYDGADLVVVNTCGFIDSAVEESLDAIGEALNENGKVIVTGCL
GAKGDVVRDVHPSVLAVTGPHATEEVMSAVHTHLPKPHDPFVDLVPDIGVRLTPKHYAYLKISEGCNHRCTFCIIPSMRG
DLESRPIHDVLREAESLAKAGVKEILVISQDTSAYGVDTKYKLGFHNGRPVKTRMTELCEELGRHGIWVRLHYVYPYPHV
DEVIPLMRDGKILPYLDIPFQHASQKVLKLMKRPANSDNVLARIKKWREICPELVIRSTFIVGFPGETEEDFEELLAFIR
EAELDRVGCFTYSPVEGATANELPNPVPEDVKEARKERFMAVQAEISARRLERRVGQTLQVLVDEIDDEGTAVCRSYADA
PEIDGLVFVEDAAGMQPGEFYQVEIVDCSEHDLWGERR
>Mature_438_residues
MNKTPRVGFVSLGCPKAASDSEQILTRLRAEGYEIAPSYDGADLVVVNTCGFIDSAVEESLDAIGEALNENGKVIVTGCL
GAKGDVVRDVHPSVLAVTGPHATEEVMSAVHTHLPKPHDPFVDLVPDIGVRLTPKHYAYLKISEGCNHRCTFCIIPSMRG
DLESRPIHDVLREAESLAKAGVKEILVISQDTSAYGVDTKYKLGFHNGRPVKTRMTELCEELGRHGIWVRLHYVYPYPHV
DEVIPLMRDGKILPYLDIPFQHASQKVLKLMKRPANSDNVLARIKKWREICPELVIRSTFIVGFPGETEEDFEELLAFIR
EAELDRVGCFTYSPVEGATANELPNPVPEDVKEARKERFMAVQAEISARRLERRVGQTLQVLVDEIDDEGTAVCRSYADA
PEIDGLVFVEDAAGMQPGEFYQVEIVDCSEHDLWGERR

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=489, Percent_Identity=25.5623721881391, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI93277076, Length=394, Percent_Identity=27.6649746192893, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI28872782, Length=489, Percent_Identity=25.5623721881391, Blast_Score=136, Evalue=5e-32,
Organism=Escherichia coli, GI1787057, Length=434, Percent_Identity=69.3548387096774, Blast_Score=640, Evalue=0.0,
Organism=Escherichia coli, GI1786882, Length=453, Percent_Identity=28.9183222958057, Blast_Score=155, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17553146, Length=472, Percent_Identity=24.5762711864407, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71996771, Length=264, Percent_Identity=30.3030303030303, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356207, Length=423, Percent_Identity=25.7683215130024, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI19922432, Length=402, Percent_Identity=27.363184079602, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_CHRVO (Q7NYA1)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901043.1
- ProteinModelPortal:   Q7NYA1
- SMR:   Q7NYA1
- GeneID:   2550871
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1373
- NMPDR:   fig|243365.1.peg.1373
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   Q7NYA1
- BioCyc:   CVIO243365:CV_1373-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 48761; Mature: 48761

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTPRVGFVSLGCPKAASDSEQILTRLRAEGYEIAPSYDGADLVVVNTCGFIDSAVEES
CCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCEEECCCCCCCCEEEEECCCHHHHHHHHH
LDAIGEALNENGKVIVTGCLGAKGDVVRDVHPSVLAVTGPHATEEVMSAVHTHLPKPHDP
HHHHHHHHCCCCCEEEEECCCCCCCCHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCC
FVDLVPDIGVRLTPKHYAYLKISEGCNHRCTFCIIPSMRGDLESRPIHDVLREAESLAKA
HHHHHCCCCCEECCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHC
GVKEILVISQDTSAYGVDTKYKLGFHNGRPVKTRMTELCEELGRHGIWVRLHYVYPYPHV
CHHEEEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCH
DEVIPLMRDGKILPYLDIPFQHASQKVLKLMKRPANSDNVLARIKKWREICPELVIRSTF
HHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEE
IVGFPGETEEDFEELLAFIREAELDRVGCFTYSPVEGATANELPNPVPEDVKEARKERFM
EEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHHH
AVQAEISARRLERRVGQTLQVLVDEIDDEGTAVCRSYADAPEIDGLVFVEDAAGMQPGEF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCE
YQVEIVDCSEHDLWGERR
EEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MNKTPRVGFVSLGCPKAASDSEQILTRLRAEGYEIAPSYDGADLVVVNTCGFIDSAVEES
CCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCEEECCCCCCCCEEEEECCCHHHHHHHHH
LDAIGEALNENGKVIVTGCLGAKGDVVRDVHPSVLAVTGPHATEEVMSAVHTHLPKPHDP
HHHHHHHHCCCCCEEEEECCCCCCCCHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCC
FVDLVPDIGVRLTPKHYAYLKISEGCNHRCTFCIIPSMRGDLESRPIHDVLREAESLAKA
HHHHHCCCCCEECCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHC
GVKEILVISQDTSAYGVDTKYKLGFHNGRPVKTRMTELCEELGRHGIWVRLHYVYPYPHV
CHHEEEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCH
DEVIPLMRDGKILPYLDIPFQHASQKVLKLMKRPANSDNVLARIKKWREICPELVIRSTF
HHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEE
IVGFPGETEEDFEELLAFIREAELDRVGCFTYSPVEGATANELPNPVPEDVKEARKERFM
EEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHHH
AVQAEISARRLERRVGQTLQVLVDEIDDEGTAVCRSYADAPEIDGLVFVEDAAGMQPGEF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCE
YQVEIVDCSEHDLWGERR
EEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782