Click here to switch to the map view.

The map label for this gene is pcaD [H]

Identifier: 34496786

GI number: 34496786

Start: 1401360

End: 1402148

Strand: Reverse

Name: pcaD [H]

Synonym: CV_1331

Alternate gene names: 34496786

Gene position: 1402148-1401360 (Counterclockwise)

Preceding gene: 34496794

Following gene: 34496785

Centisome position: 29.51

GC content: 68.31

Gene sequence:

>789_bases
ATGCCGCACGCCCAGCTAGCCGGATACCAGGTCTACTACGAGGAAAGCGGCCACGGCAGCCAGCCCCTGCTGCTGCTGAA
CGGCATCACCATGTCCACCGTGGGCTGGACGCTGATGATGCCCTTGCTGGAACCGCACTTCCGGGTGATCCGCATGGATT
TCCTCGGCCAGGGGCAGAGCGAACACCCGCCGGGCGAACTCTACGCGCTGGCCGACCAGGCCGACCTGGCCGCCGCGCTG
CTGGACTGGCTGCAGCTGCCTCAGGCTTACGTGGCCGGCCTGTCGTACGGCGGCATGGTGGCCCAGCACCTGGCGCACCG
CCATCCCGGCCGGCTGAGCCGCCTGCTGCTGGCCGGCACGCTGGCCTGGGCCGATACCGTCAACCATCACATCGGCGACA
GCTGGATCGCCGCCAACAACCAGGGCGGGCTCGATCTGCGCTACCAGGTCAGCGTGCCCTGGCTGTTCTCCAGCCGCTTC
CTGGCCGGCAACGCCGCGATGCTGGACGATCTGAAGCTGCTGGCGGGCATGGTGGACTGGGATGCGGTGATCCGGCTGAT
CAACGGCGTCCGCCAACACGACGCGCGGACCTGGCTGCCGCAATTGCAACTCCCGGTGCATATACTGGTCGGAGACGAAG
ACCGGCTGACGCCGCTGTACCAGGCGCGGCTGCTGCAGGAACTGATACCGGGCGCGGAGCTGGAAGTGCTGCCCGGCGCC
GCCCACGTGCTGCACATCGAAGCCGTGGAAGCCTTCGCCCGCGCCATCGTCCGCTTCCGCCAGGACTGA

Upstream 100 bases:

>100_bases
ATCGACATTACAAATGGGATTTACGCCCGCCACCTGTTCAGCCTAAGGCCGCCGGTTCTAGAATGAATGTGACGATACCG
CCAGGACAGGAGCGCCCGCC

Downstream 100 bases:

>100_bases
GGCGGCCAACCATTCTTTCAAGACAGGGGGAAACATGATGAGCACCGACGCCGTAACGCTGAGCGTGGACAACCGTGGCA
TCGCCACCGTCGCGCTGAAC

Product: 3-oxoadipate enol-lactonase

Products: 3-oxoadipate

Alternate protein names: Aminohydrolase [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MPHAQLAGYQVYYEESGHGSQPLLLLNGITMSTVGWTLMMPLLEPHFRVIRMDFLGQGQSEHPPGELYALADQADLAAAL
LDWLQLPQAYVAGLSYGGMVAQHLAHRHPGRLSRLLLAGTLAWADTVNHHIGDSWIAANNQGGLDLRYQVSVPWLFSSRF
LAGNAAMLDDLKLLAGMVDWDAVIRLINGVRQHDARTWLPQLQLPVHILVGDEDRLTPLYQARLLQELIPGAELEVLPGA
AHVLHIEAVEAFARAIVRFRQD

Sequences:

>Translated_262_residues
MPHAQLAGYQVYYEESGHGSQPLLLLNGITMSTVGWTLMMPLLEPHFRVIRMDFLGQGQSEHPPGELYALADQADLAAAL
LDWLQLPQAYVAGLSYGGMVAQHLAHRHPGRLSRLLLAGTLAWADTVNHHIGDSWIAANNQGGLDLRYQVSVPWLFSSRF
LAGNAAMLDDLKLLAGMVDWDAVIRLINGVRQHDARTWLPQLQLPVHILVGDEDRLTPLYQARLLQELIPGAELEVLPGA
AHVLHIEAVEAFARAIVRFRQD
>Mature_261_residues
PHAQLAGYQVYYEESGHGSQPLLLLNGITMSTVGWTLMMPLLEPHFRVIRMDFLGQGQSEHPPGELYALADQADLAAALL
DWLQLPQAYVAGLSYGGMVAQHLAHRHPGRLSRLLLAGTLAWADTVNHHIGDSWIAANNQGGLDLRYQVSVPWLFSSRFL
AGNAAMLDDLKLLAGMVDWDAVIRLINGVRQHDARTWLPQLQLPVHILVGDEDRLTPLYQARLLQELIPGAELEVLPGAA
HVLHIEAVEAFARAIVRFRQD

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR019913 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.1.24

Molecular weight: Translated: 29016; Mature: 28885

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHAQLAGYQVYYEESGHGSQPLLLLNGITMSTVGWTLMMPLLEPHFRVIRMDFLGQGQS
CCCCCCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHEEEEHHHCCCCC
EHPPGELYALADQADLAAALLDWLQLPQAYVAGLSYGGMVAQHLAHRHPGRLSRLLLAGT
CCCCCCEEEECCHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHH
LAWADTVNHHIGDSWIAANNQGGLDLRYQVSVPWLFSSRFLAGNAAMLDDLKLLAGMVDW
HHHHHHHHHHCCCCEEEECCCCCEEEEEEEECCEEECCCCCCCCHHHHHHHHHHHHHCCH
DAVIRLINGVRQHDARTWLPQLQLPVHILVGDEDRLTPLYQARLLQELIPGAELEVLPGA
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECCC
AHVLHIEAVEAFARAIVRFRQD
HHEEEHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PHAQLAGYQVYYEESGHGSQPLLLLNGITMSTVGWTLMMPLLEPHFRVIRMDFLGQGQS
CCCCCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHEEEEHHHCCCCC
EHPPGELYALADQADLAAALLDWLQLPQAYVAGLSYGGMVAQHLAHRHPGRLSRLLLAGT
CCCCCCEEEECCHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHH
LAWADTVNHHIGDSWIAANNQGGLDLRYQVSVPWLFSSRFLAGNAAMLDDLKLLAGMVDW
HHHHHHHHHHCCCCEEEECCCCCEEEEEEEECCEEECCCCCCCCHHHHHHHHHHHHHCCH
DAVIRLINGVRQHDARTWLPQLQLPVHILVGDEDRLTPLYQARLLQELIPGAELEVLPGA
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECCC
AHVLHIEAVEAFARAIVRFRQD
HHEEEHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxoadipate enol-lactone; H2O

Specific reaction: 3-oxoadipate enol-lactone + H2O = 3-oxoadipate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA