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The map label for this gene is sbcB [H]

Identifier: 34496784

GI number: 34496784

Start: 1398832

End: 1400250

Strand: Reverse

Name: sbcB [H]

Synonym: CV_1329

Alternate gene names: 34496784

Gene position: 1400250-1398832 (Counterclockwise)

Preceding gene: 34496785

Following gene: 34496783

Centisome position: 29.47

GC content: 68.92

Gene sequence:

>1419_bases
ATGTCCAATCACACGTTCTTCTGGCACGACTACGAAACCTTCGGCGCCGTGCCGCGCCAGGACCGCCCGTCGCAGTTCGC
CGGCATCCGCACCGACGCCGAGCTGAACGAGATCGGCGAGCCGGTGATGCTGTACTGCCGCCCCGCGCCCGATTACCTGC
CCACGCTGCAGGCCTGCCTGCTGACCGGCATCACGCCGCAGCTGTGCCGGCAGCAGGGCGTGGCGGAACACGAGTTCGCC
GGCGTGATCGAGCGCGAGCTGGCCACGCCCGCCACCATAGGCGTCGGCTACAACTCGCTGCGCTTCGACGACGAGGTCAC
CCGCTTCCTGTTCTGGCGCAACCTGATCGACCCCTACGCCCGCGAATGGCAGAACCACTGCGGCCGCTGGGACCTGCTGG
ACCTGGTCCGCGCCACCTACGCGCTGCGCCCCGACGGCATCGTCTGGCCCAAGCACGAGGACGGCCGGACCAGCTTCAAG
CTGGAGCACCTGTCCGCCGCCAACGGGCTGGCGCATGCGTCGGCCCACGACGCGTTGTCCGACGTGCGCGCCACCATCGC
GCTGGCGCGGCTGATCCGCCAGCGCCAGCCCAAGCTGTTCGACTACTACCTGACGCTGCGCAAGAAGGACGCGGTCAAGG
TGCAGCTGTCGCTGCACGACCCCAAGCCGGTGCTGCACGTCTCCGGCATGTACGGCGTGGAGCGCGGCAATCTGGCCGTG
GTCTGGCCGCTGGCCGCCCACCCGACCAATGCCAACGAGGTCATCGTCTGGGACCTGGCCCACGACCCGGCCGAACTGCG
CGGCCTCTCCGCCGACGACATCCGCCAGCGCATGTTCACGCGCGCCGACGAACTGCCCGAAGGCGTGGCGCGGCTGCCGG
TCAAGACCATCCACATCAACAAGTCGCCGTTCGTGGTGGCCAATCTGAAGGCGCTGGGCGACGACAACGCCGCGCGCTGG
GGCATGGACCTGGCCGCCATCCACCGCCACGCCGAGGCCGCCCGCGCGCTGCCAGACCTGTCGGCGCTGTGGCGCCGCGT
TTATCAACGCGAAGCCGGCGAGCCGCAAGATGTGGATCAAAACCTCTACGGCGGCTTCGTCTCCAACAACGACCGCAAGG
CGCTGCAGAAAATGCGCCGGCTGTCCGCCGCCCAGTTGGCCGGCGAGATGGCGCTGTTCGAGGACCCGCAGCTGGCCGAG
CTGCTGTTCCGCTACCGCGCCCGCAACTTCCCGGACACCTTGTCCGGCGACGAGCAAAAGCGCTGGAAAGCCTGGTGCCA
GGCCAGGCTGGCCCAATCCTGGCCGGCGTTCCAGCAGGAGCTGGCCGAACTGCGGGCCGGCGAACTGACCGATGGCCAGC
GCAACCTGCTGCAACAAGTGGCCGACTACGCCGCCAGTCTTCAGGCCAGTCTGGCCTGA

Upstream 100 bases:

>100_bases
GGGCGCCGCGTCGGAATCCCCCCGCATGAGGCGCGGAGGCTGGCCCGACCTGCGGCGACGGTGCGACAATAGCGTCGTTT
ACCGTTTGCCGCCTCCATCC

Downstream 100 bases:

>100_bases
CCCTCCGGCCGGCGGCCTCGCCGGCCGCCTCCGCCTTCCCTCATCCTGACCAGATACGCGTTTATGCGTATGAATTTCTA
CTAGCCGATGAACTAGAACG

Product: exonuclease I

Products: NA

Alternate protein names: Exonuclease I; DNA deoxyribophosphodiesterase; dRPase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSNHTFFWHDYETFGAVPRQDRPSQFAGIRTDAELNEIGEPVMLYCRPAPDYLPTLQACLLTGITPQLCRQQGVAEHEFA
GVIERELATPATIGVGYNSLRFDDEVTRFLFWRNLIDPYAREWQNHCGRWDLLDLVRATYALRPDGIVWPKHEDGRTSFK
LEHLSAANGLAHASAHDALSDVRATIALARLIRQRQPKLFDYYLTLRKKDAVKVQLSLHDPKPVLHVSGMYGVERGNLAV
VWPLAAHPTNANEVIVWDLAHDPAELRGLSADDIRQRMFTRADELPEGVARLPVKTIHINKSPFVVANLKALGDDNAARW
GMDLAAIHRHAEAARALPDLSALWRRVYQREAGEPQDVDQNLYGGFVSNNDRKALQKMRRLSAAQLAGEMALFEDPQLAE
LLFRYRARNFPDTLSGDEQKRWKAWCQARLAQSWPAFQQELAELRAGELTDGQRNLLQQVADYAASLQASLA

Sequences:

>Translated_472_residues
MSNHTFFWHDYETFGAVPRQDRPSQFAGIRTDAELNEIGEPVMLYCRPAPDYLPTLQACLLTGITPQLCRQQGVAEHEFA
GVIERELATPATIGVGYNSLRFDDEVTRFLFWRNLIDPYAREWQNHCGRWDLLDLVRATYALRPDGIVWPKHEDGRTSFK
LEHLSAANGLAHASAHDALSDVRATIALARLIRQRQPKLFDYYLTLRKKDAVKVQLSLHDPKPVLHVSGMYGVERGNLAV
VWPLAAHPTNANEVIVWDLAHDPAELRGLSADDIRQRMFTRADELPEGVARLPVKTIHINKSPFVVANLKALGDDNAARW
GMDLAAIHRHAEAARALPDLSALWRRVYQREAGEPQDVDQNLYGGFVSNNDRKALQKMRRLSAAQLAGEMALFEDPQLAE
LLFRYRARNFPDTLSGDEQKRWKAWCQARLAQSWPAFQQELAELRAGELTDGQRNLLQQVADYAASLQASLA
>Mature_471_residues
SNHTFFWHDYETFGAVPRQDRPSQFAGIRTDAELNEIGEPVMLYCRPAPDYLPTLQACLLTGITPQLCRQQGVAEHEFAG
VIERELATPATIGVGYNSLRFDDEVTRFLFWRNLIDPYAREWQNHCGRWDLLDLVRATYALRPDGIVWPKHEDGRTSFKL
EHLSAANGLAHASAHDALSDVRATIALARLIRQRQPKLFDYYLTLRKKDAVKVQLSLHDPKPVLHVSGMYGVERGNLAVV
WPLAAHPTNANEVIVWDLAHDPAELRGLSADDIRQRMFTRADELPEGVARLPVKTIHINKSPFVVANLKALGDDNAARWG
MDLAAIHRHAEAARALPDLSALWRRVYQREAGEPQDVDQNLYGGFVSNNDRKALQKMRRLSAAQLAGEMALFEDPQLAEL
LFRYRARNFPDTLSGDEQKRWKAWCQARLAQSWPAFQQELAELRAGELTDGQRNLLQQVADYAASLQASLA

Specific function: Also functions as a DNA deoxyribophosphodiesterase that release deoxyribose-phosphate moieties following the cleavage DNA at an apurinic/apyrimidinic (AP) site by either an AP endonuclease AP lyase [H]

COG id: COG2925

COG function: function code L; Exonuclease I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788321, Length=460, Percent_Identity=46.304347826087, Blast_Score=387, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013620
- InterPro:   IPR013520
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T; PF08411 Exonuc_X-T_C [H]

EC number: =3.1.11.1 [H]

Molecular weight: Translated: 53367; Mature: 53236

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNHTFFWHDYETFGAVPRQDRPSQFAGIRTDAELNEIGEPVMLYCRPAPDYLPTLQACL
CCCCCEEEECHHHHCCCCCCCCCCHHCCCCCCCHHHHCCCCEEEEEECCCCHHHHHHHHH
LTGITPQLCRQQGVAEHEFAGVIERELATPATIGVGYNSLRFDDEVTRFLFWRNLIDPYA
HHCCCHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHH
REWQNHCGRWDLLDLVRATYALRPDGIVWPKHEDGRTSFKLEHLSAANGLAHASAHDALS
HHHHHHCCCCHHHHHHHHHHHCCCCCEECCCCCCCCCCEEEHHHHHHCCHHHHHHHHHHH
DVRATIALARLIRQRQPKLFDYYLTLRKKDAVKVQLSLHDPKPVLHVSGMYGVERGNLAV
HHHHHHHHHHHHHHHCCCCEEHHHHEECCCEEEEEEEECCCCCEEEECCCCEECCCCEEE
VWPLAAHPTNANEVIVWDLAHDPAELRGLSADDIRQRMFTRADELPEGVARLPVKTIHIN
EEEEECCCCCCCEEEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEC
KSPFVVANLKALGDDNAARWGMDLAAIHRHAEAARALPDLSALWRRVYQREAGEPQDVDQ
CCCEEEEEEHHHCCCCHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCH
NLYGGFVSNNDRKALQKMRRLSAAQLAGEMALFEDPQLAELLFRYRARNFPDTLSGDEQK
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHH
RWKAWCQARLAQSWPAFQQELAELRAGELTDGQRNLLQQVADYAASLQASLA
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNHTFFWHDYETFGAVPRQDRPSQFAGIRTDAELNEIGEPVMLYCRPAPDYLPTLQACL
CCCCEEEECHHHHCCCCCCCCCCHHCCCCCCCHHHHCCCCEEEEEECCCCHHHHHHHHH
LTGITPQLCRQQGVAEHEFAGVIERELATPATIGVGYNSLRFDDEVTRFLFWRNLIDPYA
HHCCCHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHH
REWQNHCGRWDLLDLVRATYALRPDGIVWPKHEDGRTSFKLEHLSAANGLAHASAHDALS
HHHHHHCCCCHHHHHHHHHHHCCCCCEECCCCCCCCCCEEEHHHHHHCCHHHHHHHHHHH
DVRATIALARLIRQRQPKLFDYYLTLRKKDAVKVQLSLHDPKPVLHVSGMYGVERGNLAV
HHHHHHHHHHHHHHHCCCCEEHHHHEECCCEEEEEEEECCCCCEEEECCCCEECCCCEEE
VWPLAAHPTNANEVIVWDLAHDPAELRGLSADDIRQRMFTRADELPEGVARLPVKTIHIN
EEEEECCCCCCCEEEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEC
KSPFVVANLKALGDDNAARWGMDLAAIHRHAEAARALPDLSALWRRVYQREAGEPQDVDQ
CCCEEEEEEHHHCCCCHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCH
NLYGGFVSNNDRKALQKMRRLSAAQLAGEMALFEDPQLAELLFRYRARNFPDTLSGDEQK
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHH
RWKAWCQARLAQSWPAFQQELAELRAGELTDGQRNLLQQVADYAASLQASLA
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]