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The map label for this gene is proP [H]

Identifier: 34496754

GI number: 34496754

Start: 1367547

End: 1369151

Strand: Reverse

Name: proP [H]

Synonym: CV_1299

Alternate gene names: 34496754

Gene position: 1369151-1367547 (Counterclockwise)

Preceding gene: 34496755

Following gene: 34496753

Centisome position: 28.82

GC content: 64.42

Gene sequence:

>1605_bases
ATGAGCAACAGCATCGCATCGACGACGACGTCGGCCGGCATCAGCCGCGCCGAACGCAAGGTGATACTCGCCTCGTCGCT
CGGCACGGTATTCGAGTGGTACGACTTCTTCCTGTACGGCGCGCTGGCCGCCATCATCGGCAAGCAGTTCTTCGCCGGCG
TCAACGAGACCACCGCCTTCATCTTCGCGCTGATGACCTTCGCCGCCGGCTTCGTGGTGAGGCCGTTCGGCGCGCTGGTG
TTCGGCCGTCTGGGCGACATGGTGGGCCGCAAGTACACCTTCCTCGCCACCATCGTCATCATGGGCCTGTCCACCTTTCT
CGTCGGCGTGCTGCCGGGCTACGCGACGCTGGGCATCGCCGCTCCGCTGATCCTGGTATCCCTGCGCATGCTGCAGGGCC
TGGCCATCGGCGGCGAATACGGCGGCGCGGCCATCTACGTGGCCGAGCACGCGCCGGATCACAAGCGCGGCGGCTACACC
AGCTGGATCCAGGTGACCGCCGGCGCCGGCCTGCTGCTGGTGATCCTGGCCTGCCGCAAGCTGACCGGCGAGGCGTTCAA
CGAATGGGGATGGCGGCTGCCCTTCCTACTGTCCATCTTCCTGCTGGCCATCTCCACCTGGATCCGGCTGTCGATGCAGG
AGTCTCCGGCCTTCCTGAAGATGAAGGCGGAAGGCAAGCACTCCAAGGCGCCGATCAGCGAGGCCTTCGGCAACTGGCGC
AACCTGAAGATTGTGCTGATCTCGCTGTTCGGCTTCAATGGCGGCCAGGCCGTCACCTTCTACTGCGCGCAGTTCTACTC
GCTGTTCTTCCTGACGCAGATCTTGAAGGTGGACCCGCAGACCGCCAACCTGATGCTGATCGCGTCGCTGATCCTCACCA
CGCCGCTGTTCCTGTATTTCGGCCACCTGTCGGACCGCATCGGCCGCAAACCGGTGCTGATCGCCGGCTTGGCGCTAGGC
CTGGCGCTGACCTTCCCCGCCTTCCGCTGGCTGACCGACTACGCCAATCCGGACGTCGCCGCCGCCGAGGCCAGCTCTCC
GGTGACCGTGGTCGCGGATCCGGCCAGTTGCCATTTCCAGTTCGACCCCATCGGCAAAGCCAAGCTGAACACCCCATGCG
ACCAGGCCAAGGCGCTGCTGGCGAAGAACGGCGTGCCCTACCGGCAAAAGGCCGCGCCGGCCGGCGTGACCGAAGTCCAC
ATCGGCGCAACCGTTCAGCAGGGGTTCGACAAGAAAGCGCTGCAAGACGCGCTGAAAACCGCCGGCTATCCGGAAAAGGC
CGACCCCGGCAAGATCAACCACGCCATGGTGATCCTGGTGCTGGTGCTGCTGTCGGTGATCTCCGCGCTCACTTACGGAC
CGCTGGCCGCGATGATGGTGGAACTGTTTCCCACCCGCATCCGCTATACCTCGATGTCGCTGCCCTATCACCTGGGCAAC
GGCTGGATAGGCGGGCTGATGCCCACCGTCGCCTTCTCGCTGGTGGTGTACACCGGCGACATACTGTACGGACTGTGGTA
CCCGATCGTGGTCTACGCCGTCAGCCTCGCTGTAAGCCTGCTGTTCCTGAAGGAAACCTTCCGCAACGATATCCATCGCC
ACTGA

Upstream 100 bases:

>100_bases
CGCTGATGATCGCCGCCAAGGCGGCGCAGATGATCGCGCAGGACAACAAGCAAGCGGGACCGATACGAACGCCCGCTCCA
CCCGAAAAGGAGACCTGGAC

Downstream 100 bases:

>100_bases
ACCTTGCCCCCGCGGCGGCCCCCGTTATCCGCGGGGGCTTTTTCATTCCTTCCGCGCGCGCCTCCCGCGAACGGTCCGCC
CGACCACGCCTCTCCTCCAG

Product: MFS family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MSNSIASTTTSAGISRAERKVILASSLGTVFEWYDFFLYGALAAIIGKQFFAGVNETTAFIFALMTFAAGFVVRPFGALV
FGRLGDMVGRKYTFLATIVIMGLSTFLVGVLPGYATLGIAAPLILVSLRMLQGLAIGGEYGGAAIYVAEHAPDHKRGGYT
SWIQVTAGAGLLLVILACRKLTGEAFNEWGWRLPFLLSIFLLAISTWIRLSMQESPAFLKMKAEGKHSKAPISEAFGNWR
NLKIVLISLFGFNGGQAVTFYCAQFYSLFFLTQILKVDPQTANLMLIASLILTTPLFLYFGHLSDRIGRKPVLIAGLALG
LALTFPAFRWLTDYANPDVAAAEASSPVTVVADPASCHFQFDPIGKAKLNTPCDQAKALLAKNGVPYRQKAAPAGVTEVH
IGATVQQGFDKKALQDALKTAGYPEKADPGKINHAMVILVLVLLSVISALTYGPLAAMMVELFPTRIRYTSMSLPYHLGN
GWIGGLMPTVAFSLVVYTGDILYGLWYPIVVYAVSLAVSLLFLKETFRNDIHRH

Sequences:

>Translated_534_residues
MSNSIASTTTSAGISRAERKVILASSLGTVFEWYDFFLYGALAAIIGKQFFAGVNETTAFIFALMTFAAGFVVRPFGALV
FGRLGDMVGRKYTFLATIVIMGLSTFLVGVLPGYATLGIAAPLILVSLRMLQGLAIGGEYGGAAIYVAEHAPDHKRGGYT
SWIQVTAGAGLLLVILACRKLTGEAFNEWGWRLPFLLSIFLLAISTWIRLSMQESPAFLKMKAEGKHSKAPISEAFGNWR
NLKIVLISLFGFNGGQAVTFYCAQFYSLFFLTQILKVDPQTANLMLIASLILTTPLFLYFGHLSDRIGRKPVLIAGLALG
LALTFPAFRWLTDYANPDVAAAEASSPVTVVADPASCHFQFDPIGKAKLNTPCDQAKALLAKNGVPYRQKAAPAGVTEVH
IGATVQQGFDKKALQDALKTAGYPEKADPGKINHAMVILVLVLLSVISALTYGPLAAMMVELFPTRIRYTSMSLPYHLGN
GWIGGLMPTVAFSLVVYTGDILYGLWYPIVVYAVSLAVSLLFLKETFRNDIHRH
>Mature_533_residues
SNSIASTTTSAGISRAERKVILASSLGTVFEWYDFFLYGALAAIIGKQFFAGVNETTAFIFALMTFAAGFVVRPFGALVF
GRLGDMVGRKYTFLATIVIMGLSTFLVGVLPGYATLGIAAPLILVSLRMLQGLAIGGEYGGAAIYVAEHAPDHKRGGYTS
WIQVTAGAGLLLVILACRKLTGEAFNEWGWRLPFLLSIFLLAISTWIRLSMQESPAFLKMKAEGKHSKAPISEAFGNWRN
LKIVLISLFGFNGGQAVTFYCAQFYSLFFLTQILKVDPQTANLMLIASLILTTPLFLYFGHLSDRIGRKPVLIAGLALGL
ALTFPAFRWLTDYANPDVAAAEASSPVTVVADPASCHFQFDPIGKAKLNTPCDQAKALLAKNGVPYRQKAAPAGVTEVHI
GATVQQGFDKKALQDALKTAGYPEKADPGKINHAMVILVLVLLSVISALTYGPLAAMMVELFPTRIRYTSMSLPYHLGNG
WIGGLMPTVAFSLVVYTGDILYGLWYPIVVYAVSLAVSLLFLKETFRNDIHRH

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=324, Percent_Identity=38.2716049382716, Blast_Score=207, Evalue=9e-55,
Organism=Escherichia coli, GI1788292, Length=344, Percent_Identity=38.0813953488372, Blast_Score=199, Evalue=5e-52,
Organism=Escherichia coli, GI1788942, Length=353, Percent_Identity=32.5779036827195, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1789941, Length=331, Percent_Identity=36.8580060422961, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI87082231, Length=232, Percent_Identity=25.8620689655172, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI87082404, Length=316, Percent_Identity=22.7848101265823, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 57839; Mature: 57707

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSIASTTTSAGISRAERKVILASSLGTVFEWYDFFLYGALAAIIGKQFFAGVNETTAF
CCCCCCCCHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
IFALMTFAAGFVVRPFGALVFGRLGDMVGRKYTFLATIVIMGLSTFLVGVLPGYATLGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
APLILVSLRMLQGLAIGGEYGGAAIYVAEHAPDHKRGGYTSWIQVTAGAGLLLVILACRK
HHHHHHHHHHHHHEEECCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHH
LTGEAFNEWGWRLPFLLSIFLLAISTWIRLSMQESPAFLKMKAEGKHSKAPISEAFGNWR
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHCCCC
NLKIVLISLFGFNGGQAVTFYCAQFYSLFFLTQILKVDPQTANLMLIASLILTTPLFLYF
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
GHLSDRIGRKPVLIAGLALGLALTFPAFRWLTDYANPDVAAAEASSPVTVVADPASCHFQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCEEEEECCCCCEEE
FDPIGKAKLNTPCDQAKALLAKNGVPYRQKAAPAGVTEVHIGATVQQGFDKKALQDALKT
ECCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCCCCEEEECHHHHHCCCHHHHHHHHHH
AGYPEKADPGKINHAMVILVLVLLSVISALTYGPLAAMMVELFPTRIRYTSMSLPYHLGN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEECCC
GWIGGLMPTVAFSLVVYTGDILYGLWYPIVVYAVSLAVSLLFLKETFRNDIHRH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNSIASTTTSAGISRAERKVILASSLGTVFEWYDFFLYGALAAIIGKQFFAGVNETTAF
CCCCCCCHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
IFALMTFAAGFVVRPFGALVFGRLGDMVGRKYTFLATIVIMGLSTFLVGVLPGYATLGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
APLILVSLRMLQGLAIGGEYGGAAIYVAEHAPDHKRGGYTSWIQVTAGAGLLLVILACRK
HHHHHHHHHHHHHEEECCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHH
LTGEAFNEWGWRLPFLLSIFLLAISTWIRLSMQESPAFLKMKAEGKHSKAPISEAFGNWR
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHCCCC
NLKIVLISLFGFNGGQAVTFYCAQFYSLFFLTQILKVDPQTANLMLIASLILTTPLFLYF
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
GHLSDRIGRKPVLIAGLALGLALTFPAFRWLTDYANPDVAAAEASSPVTVVADPASCHFQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCEEEEECCCCCEEE
FDPIGKAKLNTPCDQAKALLAKNGVPYRQKAAPAGVTEVHIGATVQQGFDKKALQDALKT
ECCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCCCCEEEECHHHHHCCCHHHHHHHHHH
AGYPEKADPGKINHAMVILVLVLLSVISALTYGPLAAMMVELFPTRIRYTSMSLPYHLGN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEECCC
GWIGGLMPTVAFSLVVYTGDILYGLWYPIVVYAVSLAVSLLFLKETFRNDIHRH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]