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The map label for this gene is gltJ [H]

Identifier: 34496729

GI number: 34496729

Start: 1340090

End: 1340830

Strand: Reverse

Name: gltJ [H]

Synonym: CV_1274

Alternate gene names: 34496729

Gene position: 1340830-1340090 (Counterclockwise)

Preceding gene: 34496730

Following gene: 34496728

Centisome position: 28.22

GC content: 69.64

Gene sequence:

>741_bases
ATGACCCCACCCGCCTGGCTCGGCCACAACTGGCTGGAGCCGCACTATCTCATCTGGCTGGGCAAGGGCGCGCTGCTGAC
CGGCTGGCTGTCGCTGCTGGTCATCGCCGCCGCCAGCCTGCTCGGCATCGCCCTGACCGCGCTGCTGGACGGCGGCCCGC
GGCCGCTGCGGCTGGCGACGCGGCTATTCGTCGGCCTGCACCGCAACACGCCGCTGATGGTGCAGCTCTTGCTGTGGTAT
TTCGGCGTGTCCGGCCTGCTGCCGGACGAGGCGAAAATCTGGCTGAACACCCCACGCGCGCTGATGCTGCCCTTCGATCT
GGCCCTGCCCTGGCCGTCGTTCGAATTCCTGGCCGCCTTCACCGCGCTGACGCTGTACAGCGCCGCCTTCATCAGCGGCG
AGCTGCAGGCCGGGCTGAACGCGGTGCCCAAAGGCCAGCGGCTGGCGGCGCGCGCGCTGGGCATGAGCGGCTGGCAGGTC
TTGCGCCTGGTGGTGCTGCCGCAGGCCTGGCAGCTGGTTAAGCGGCCGCTGGTCGGCCAGTACACCGCCATCGTCAAGAA
CACCTCGCTGACCATGGCCATCGGCCTGGCGGAGCTCTCTTACGCGTCGCGCCAGGTGGAGACCGAAACGCTGCTGGCCT
TCCAGTCCTTCGCCGCCGCCACCGCGCTCTACCTGGCGCTGGTGCTGATCACCCAATTGCTGGCCCAGCGGCCGGAGCCG
GCCTGGAGGACGATGCGATGA

Upstream 100 bases:

>100_bases
TTCAAGATCGGCGACGCGGCCTGATCCCAGGCTGGTCCCCGTCCGCAACCGCCGGCTCCCCCGGCGGTTTTGCCGTTTCA
TGTCGCAGAAAGAAAACCGC

Downstream 100 bases:

>100_bases
TGCCTGCGGTGATACAGGACAACCTCGACTACCTGCTGTGGGGCAATCTGGCCGGCGGCGAGCCCGGCGGCCTGCTGCTG
ACGCTGCTGCTGAGCCTGGC

Product: ABC transporter permease

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTPPAWLGHNWLEPHYLIWLGKGALLTGWLSLLVIAAASLLGIALTALLDGGPRPLRLATRLFVGLHRNTPLMVQLLLWY
FGVSGLLPDEAKIWLNTPRALMLPFDLALPWPSFEFLAAFTALTLYSAAFISGELQAGLNAVPKGQRLAARALGMSGWQV
LRLVVLPQAWQLVKRPLVGQYTAIVKNTSLTMAIGLAELSYASRQVETETLLAFQSFAAATALYLALVLITQLLAQRPEP
AWRTMR

Sequences:

>Translated_246_residues
MTPPAWLGHNWLEPHYLIWLGKGALLTGWLSLLVIAAASLLGIALTALLDGGPRPLRLATRLFVGLHRNTPLMVQLLLWY
FGVSGLLPDEAKIWLNTPRALMLPFDLALPWPSFEFLAAFTALTLYSAAFISGELQAGLNAVPKGQRLAARALGMSGWQV
LRLVVLPQAWQLVKRPLVGQYTAIVKNTSLTMAIGLAELSYASRQVETETLLAFQSFAAATALYLALVLITQLLAQRPEP
AWRTMR
>Mature_245_residues
TPPAWLGHNWLEPHYLIWLGKGALLTGWLSLLVIAAASLLGIALTALLDGGPRPLRLATRLFVGLHRNTPLMVQLLLWYF
GVSGLLPDEAKIWLNTPRALMLPFDLALPWPSFEFLAAFTALTLYSAAFISGELQAGLNAVPKGQRLAARALGMSGWQVL
RLVVLPQAWQLVKRPLVGQYTAIVKNTSLTMAIGLAELSYASRQVETETLLAFQSFAAATALYLALVLITQLLAQRPEPA
WRTMR

Specific function: Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786874, Length=225, Percent_Identity=28.8888888888889, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788226, Length=233, Percent_Identity=33.4763948497854, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI48994930, Length=120, Percent_Identity=42.5, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787030, Length=223, Percent_Identity=27.8026905829596, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787087, Length=189, Percent_Identity=29.1005291005291, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788645, Length=238, Percent_Identity=25.6302521008403, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786873, Length=181, Percent_Identity=25.9668508287293, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI87082239, Length=194, Percent_Identity=25.7731958762887, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 27006; Mature: 26875

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPPAWLGHNWLEPHYLIWLGKGALLTGWLSLLVIAAASLLGIALTALLDGGPRPLRLAT
CCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
RLFVGLHRNTPLMVQLLLWYFGVSGLLPDEAKIWLNTPRALMLPFDLALPWPSFEFLAAF
HHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEEHHHCCCCCCHHHHHHH
TALTLYSAAFISGELQAGLNAVPKGQRLAARALGMSGWQVLRLVVLPQAWQLVKRPLVGQ
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHH
YTAIVKNTSLTMAIGLAELSYASRQVETETLLAFQSFAAATALYLALVLITQLLAQRPEP
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AWRTMR
HHCCCC
>Mature Secondary Structure 
TPPAWLGHNWLEPHYLIWLGKGALLTGWLSLLVIAAASLLGIALTALLDGGPRPLRLAT
CCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
RLFVGLHRNTPLMVQLLLWYFGVSGLLPDEAKIWLNTPRALMLPFDLALPWPSFEFLAAF
HHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEEHHHCCCCCCHHHHHHH
TALTLYSAAFISGELQAGLNAVPKGQRLAARALGMSGWQVLRLVVLPQAWQLVKRPLVGQ
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHH
YTAIVKNTSLTMAIGLAELSYASRQVETETLLAFQSFAAATALYLALVLITQLLAQRPEP
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AWRTMR
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]