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The map label for this gene is bglB [H]

Identifier: 34496727

GI number: 34496727

Start: 1337973

End: 1339409

Strand: Direct

Name: bglB [H]

Synonym: CV_1272

Alternate gene names: 34496727

Gene position: 1337973-1339409 (Clockwise)

Preceding gene: 34496725

Following gene: 34496732

Centisome position: 28.16

GC content: 68.27

Gene sequence:

>1437_bases
ATGCATCACCCCAGCCTCAAGCCTTTCCCCGATGATTTCCTGTGGGGCGCCGCCTCCGCCGCCTACCAGGTGGAAGGCGC
CTTCGACGCCGACGGCAAGGGGCCGTCGGTGTGGGACGTCTTCGTCCGCGAGCCGGGCCGCACCTTTCAGGGCAGCAACG
GCGACGTGGCGGTAGACCACTATCACCGGATGGAGGAGGACGTGGCGCTGATGGCCGAGATGGGCCTGACCGCCTATCGT
TTCTCGGTCAGCTGGCCGCGCGTGCTGCCGCGGGGGCGCGGCGAGCCGAACGAGGCCGGCCTCGCCTTTTACGACAGGTT
GATCGACGCGCTGCTGGCGCGCGGCATCGAGCCGGTGCTGACGCTGTACCACTGGGACATGCCGCAGGCGCTGCAGGACG
AATACGGCGGCTGGGAAGACAGGCGCATCGTCGACGACTTCGGCGACTACTGCGCGCTGCTGTTCCGCCGCTACGGCGAC
CGGGTGAAGACCTGGGTGTCGCTGAACGAGCAGAACTACAATCTGAGCAATGCCTACCTGCTGGCGACGCATCCGCCGGC
GGCGGCCGACCGCAAGCGCTTTTACGCCGCCAACCACATCGCCTTCCTGGCCAACGCGCGGGCGATCCGGCTGTTCCGGC
AATGGGTGCCGGATGGCCGCATCGGGCCCAGCTTCGCCTATTCTCCGGCCTATCCGGCCTCCTGCGCCCCGGCGGACATG
CTGGCCTTCGACAACGCCGAGGAGTTCACCAACCACTGGTGGCTGGACGTCTACTGCCGCGGCCGCTATCCGCAGATCGC
GCTGGCCTGGCTGCGGGAGCGGGGCGAGGCGCCGGACATCCGGCCGGGCGACATCGAGGCGCTGGCGGCGGCGAGGCCCG
ATTTCATCGGACTCAATTACTATTACACCTTGAGCTACGCCCACAATCCGCCGGACGGCGTCGGCATGCAGCGAATCAAC
ACCACCGGGCAGAAGGGCAGCACGCCGGCCAGCGGCGTGCCCGGCCTGTACCGCACCGCGCCGAATCCGGCGCTGGAAAC
CTCCAATTGGGACTGGGCGATCGATCCCGCCGGTCTGCGCATCGCCTTGCGCCGCCTGACCAGCCGCTACGGGCTGCCGG
TGCTGATCACCGAGAACGGCCTGGGCGAGTTCGACCGGCTGGAGGATGGCGACCGGGTGCGCGACGACTACCGCATCGCC
TACCTGCGCAGCCATCTCCATGCCTGCCGCGAGGCGATCAGCGACGGGGTGGCGTTGCTGGGCTTCTGCGCCTGGTCGTT
CACCGACATCCTCAGCTGGCTCAACGGCTACCAGAAGCGCTACGGCTTCGTCTACGTCAACCGCGATGAGACCGACGCCC
GCGATCTGCGCCGCGTGCGCAAGGACAGCTTCTTCTGGTACCAGCGGGCGATCGCCAGCGGCGGCCGCGAACTGTGA

Upstream 100 bases:

>100_bases
CGGCGGGCTTTCCCGCCGGCGGCGCGATGCCGCATCATCGCCGAACTTGCCGGCCGCCGCGCCGACCAACAGGGCATAGC
CGTTTTCACCGGAGACCAGC

Downstream 100 bases:

>100_bases
TCAGGCTGTCCGCCGCCAGCGGCGTTCCGCCAGATGGCCGGCCAGCTGCAGCAGGCCGCACAGCAGCAGGTAGCCGATGG
CGATGGCGCTGAAGATTTCC

Product: beta-glucosidase

Products: NA

Alternate protein names: 6-phospho-beta-glucosidase [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MHHPSLKPFPDDFLWGAASAAYQVEGAFDADGKGPSVWDVFVREPGRTFQGSNGDVAVDHYHRMEEDVALMAEMGLTAYR
FSVSWPRVLPRGRGEPNEAGLAFYDRLIDALLARGIEPVLTLYHWDMPQALQDEYGGWEDRRIVDDFGDYCALLFRRYGD
RVKTWVSLNEQNYNLSNAYLLATHPPAAADRKRFYAANHIAFLANARAIRLFRQWVPDGRIGPSFAYSPAYPASCAPADM
LAFDNAEEFTNHWWLDVYCRGRYPQIALAWLRERGEAPDIRPGDIEALAAARPDFIGLNYYYTLSYAHNPPDGVGMQRIN
TTGQKGSTPASGVPGLYRTAPNPALETSNWDWAIDPAGLRIALRRLTSRYGLPVLITENGLGEFDRLEDGDRVRDDYRIA
YLRSHLHACREAISDGVALLGFCAWSFTDILSWLNGYQKRYGFVYVNRDETDARDLRRVRKDSFFWYQRAIASGGREL

Sequences:

>Translated_478_residues
MHHPSLKPFPDDFLWGAASAAYQVEGAFDADGKGPSVWDVFVREPGRTFQGSNGDVAVDHYHRMEEDVALMAEMGLTAYR
FSVSWPRVLPRGRGEPNEAGLAFYDRLIDALLARGIEPVLTLYHWDMPQALQDEYGGWEDRRIVDDFGDYCALLFRRYGD
RVKTWVSLNEQNYNLSNAYLLATHPPAAADRKRFYAANHIAFLANARAIRLFRQWVPDGRIGPSFAYSPAYPASCAPADM
LAFDNAEEFTNHWWLDVYCRGRYPQIALAWLRERGEAPDIRPGDIEALAAARPDFIGLNYYYTLSYAHNPPDGVGMQRIN
TTGQKGSTPASGVPGLYRTAPNPALETSNWDWAIDPAGLRIALRRLTSRYGLPVLITENGLGEFDRLEDGDRVRDDYRIA
YLRSHLHACREAISDGVALLGFCAWSFTDILSWLNGYQKRYGFVYVNRDETDARDLRRVRKDSFFWYQRAIASGGREL
>Mature_478_residues
MHHPSLKPFPDDFLWGAASAAYQVEGAFDADGKGPSVWDVFVREPGRTFQGSNGDVAVDHYHRMEEDVALMAEMGLTAYR
FSVSWPRVLPRGRGEPNEAGLAFYDRLIDALLARGIEPVLTLYHWDMPQALQDEYGGWEDRRIVDDFGDYCALLFRRYGD
RVKTWVSLNEQNYNLSNAYLLATHPPAAADRKRFYAANHIAFLANARAIRLFRQWVPDGRIGPSFAYSPAYPASCAPADM
LAFDNAEEFTNHWWLDVYCRGRYPQIALAWLRERGEAPDIRPGDIEALAAARPDFIGLNYYYTLSYAHNPPDGVGMQRIN
TTGQKGSTPASGVPGLYRTAPNPALETSNWDWAIDPAGLRIALRRLTSRYGLPVLITENGLGEFDRLEDGDRVRDDYRIA
YLRSHLHACREAISDGVALLGFCAWSFTDILSWLNGYQKRYGFVYVNRDETDARDLRRVRKDSFFWYQRAIASGGREL

Specific function: Is able to catalyze the hydrolysis of aryl-phospho-beta- D-glucosides such as 4-methylumbelliferyl-phospho-beta-D- glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Is not essential for growth on arbutin and salicin as the sole carbon source [H]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI110681710, Length=483, Percent_Identity=32.5051759834368, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI32481206, Length=489, Percent_Identity=34.3558282208589, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI13273313, Length=468, Percent_Identity=33.974358974359, Blast_Score=224, Evalue=2e-58,
Organism=Homo sapiens, GI24497614, Length=495, Percent_Identity=30.5050505050505, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI28376633, Length=177, Percent_Identity=44.0677966101695, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI190360571, Length=85, Percent_Identity=56.4705882352941, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI2367270, Length=483, Percent_Identity=39.9585921325052, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI2367174, Length=497, Percent_Identity=37.4245472837022, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1789070, Length=492, Percent_Identity=35.5691056910569, Blast_Score=300, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI17552856, Length=478, Percent_Identity=31.1715481171548, Blast_Score=219, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI17539390, Length=488, Percent_Identity=29.5081967213115, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI21356577, Length=459, Percent_Identity=31.5904139433551, Blast_Score=210, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 54252; Mature: 54252

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHHPSLKPFPDDFLWGAASAAYQVEGAFDADGKGPSVWDVFVREPGRTFQGSNGDVAVDH
CCCCCCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEHHH
YHRMEEDVALMAEMGLTAYRFSVSWPRVLPRGRGEPNEAGLAFYDRLIDALLARGIEPVL
HHHHHHHHHHHHHCCCEEEEEECCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHH
TLYHWDMPQALQDEYGGWEDRRIVDDFGDYCALLFRRYGDRVKTWVSLNEQNYNLSNAYL
HHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEHEEECCCCCCCCCEEE
LATHPPAAADRKRFYAANHIAFLANARAIRLFRQWVPDGRIGPSFAYSPAYPASCAPADM
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
LAFDNAEEFTNHWWLDVYCRGRYPQIALAWLRERGEAPDIRPGDIEALAAARPDFIGLNY
HCCCCHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCEEECEE
YYTLSYAHNPPDGVGMQRINTTGQKGSTPASGVPGLYRTAPNPALETSNWDWAIDPAGLR
EEEEEECCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCEEECCHHHH
IALRRLTSRYGLPVLITENGLGEFDRLEDGDRVRDDYRIAYLRSHLHACREAISDGVALL
HHHHHHHHHCCCEEEEECCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
GFCAWSFTDILSWLNGYQKRYGFVYVNRDETDARDLRRVRKDSFFWYQRAIASGGREL
HHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MHHPSLKPFPDDFLWGAASAAYQVEGAFDADGKGPSVWDVFVREPGRTFQGSNGDVAVDH
CCCCCCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEHHH
YHRMEEDVALMAEMGLTAYRFSVSWPRVLPRGRGEPNEAGLAFYDRLIDALLARGIEPVL
HHHHHHHHHHHHHCCCEEEEEECCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHH
TLYHWDMPQALQDEYGGWEDRRIVDDFGDYCALLFRRYGDRVKTWVSLNEQNYNLSNAYL
HHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEHEEECCCCCCCCCEEE
LATHPPAAADRKRFYAANHIAFLANARAIRLFRQWVPDGRIGPSFAYSPAYPASCAPADM
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
LAFDNAEEFTNHWWLDVYCRGRYPQIALAWLRERGEAPDIRPGDIEALAAARPDFIGLNY
HCCCCHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCEEECEE
YYTLSYAHNPPDGVGMQRINTTGQKGSTPASGVPGLYRTAPNPALETSNWDWAIDPAGLR
EEEEEECCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCEEECCHHHH
IALRRLTSRYGLPVLITENGLGEFDRLEDGDRVRDDYRIAYLRSHLHACREAISDGVALL
HHHHHHHHHCCCEEEEECCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
GFCAWSFTDILSWLNGYQKRYGFVYVNRDETDARDLRRVRKDSFFWYQRAIASGGREL
HHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704255; 8969502; 9384377; 2841296 [H]