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The map label for this gene is folE

Identifier: 34496585

GI number: 34496585

Start: 1183073

End: 1183693

Strand: Reverse

Name: folE

Synonym: CV_1130

Alternate gene names: 34496585

Gene position: 1183693-1183073 (Counterclockwise)

Preceding gene: 34496586

Following gene: 34496575

Centisome position: 24.91

GC content: 70.53

Gene sequence:

>621_bases
ATGTCCTTTACCGACGATCACGACCACGCCGCCTGCCAGCGCCCGCTGGAACGGGTCCGCCCGTTCGACGCCGCCGCCTT
CGAGCGGGCGGTGGCCGACATGCTGACCGCCAGCGGCATCGCCATCGATCCCATCCACACCGGCAAGACCGCCCAGCGCG
TGCGCGAGCTGTGGCAGAAGCGCCTGCTGGACGGCTACGACACCGTGCCAGCCGAAGCGCTGGGCAGCGGCTTCGCCGAC
GAGCGCCGCGACATGGTGGTGATACGCAGCCTGGCGGTGCACGGCATGTGCCCGCACCACTTGCTGCCCTTCCGCGGCGT
CGCCCACGTCGCCTACCTGCCCGGCGGCCGGCTGCACGGCTTCGGCCGCATCGCGCGCATGGTGGACGCCATCAGCCACC
GCTACACCTACCAGGAATGGATCACCCACGAGATCGCCCGCACCCTGGTCGAGCACGGCGAGGCGCGCGGCGCCGCCTGC
CTGATCGAGGCCGAGCAGCTATGCCTGCTGATGGGGGAAAACCGGCGCGGCGACGAACGGGTGATCACCCAGTGCTACGC
CGGCGACTTCGAAACCGACGCGGAAGCCCGCAACCAGTTCCTGCGCGCGATCGAGCGCTGA

Upstream 100 bases:

>100_bases
AAATAAATGGGCGAAATCTGATCGTCAGACCGGCCCCTGTCCCTATACCATGAGGGACACGAGGCCGGACGCCCTTGCTC
CGGCCGCAACGGAGCCGCCC

Downstream 100 bases:

>100_bases
GCGGCGGACAGCGAAAAGGCCGCTGCCCGTAAAAGGCAGCGGCCTTGATGTCTTTTGCGCGGGCGTCCGCCGCGCTAATC
CAGCAAGCCGCCGGATTCGG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MSFTDDHDHAACQRPLERVRPFDAAAFERAVADMLTASGIAIDPIHTGKTAQRVRELWQKRLLDGYDTVPAEALGSGFAD
ERRDMVVIRSLAVHGMCPHHLLPFRGVAHVAYLPGGRLHGFGRIARMVDAISHRYTYQEWITHEIARTLVEHGEARGAAC
LIEAEQLCLLMGENRRGDERVITQCYAGDFETDAEARNQFLRAIER

Sequences:

>Translated_206_residues
MSFTDDHDHAACQRPLERVRPFDAAAFERAVADMLTASGIAIDPIHTGKTAQRVRELWQKRLLDGYDTVPAEALGSGFAD
ERRDMVVIRSLAVHGMCPHHLLPFRGVAHVAYLPGGRLHGFGRIARMVDAISHRYTYQEWITHEIARTLVEHGEARGAAC
LIEAEQLCLLMGENRRGDERVITQCYAGDFETDAEARNQFLRAIER
>Mature_205_residues
SFTDDHDHAACQRPLERVRPFDAAAFERAVADMLTASGIAIDPIHTGKTAQRVRELWQKRLLDGYDTVPAEALGSGFADE
RRDMVVIRSLAVHGMCPHHLLPFRGVAHVAYLPGGRLHGFGRIARMVDAISHRYTYQEWITHEIARTLVEHGEARGAACL
IEAEQLCLLMGENRRGDERVITQCYAGDFETDAEARNQFLRAIER

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=141, Percent_Identity=31.9148936170213, Blast_Score=95, Evalue=4e-20,
Organism=Homo sapiens, GI4503949, Length=141, Percent_Identity=31.9148936170213, Blast_Score=95, Evalue=4e-20,
Organism=Homo sapiens, GI66932970, Length=123, Percent_Identity=30.0813008130081, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI66932972, Length=101, Percent_Identity=34.6534653465347, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560486, Length=143, Percent_Identity=33.5664335664336, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321706, Length=129, Percent_Identity=28.6821705426357, Blast_Score=83, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24656796, Length=142, Percent_Identity=30.9859154929577, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24656791, Length=142, Percent_Identity=30.9859154929577, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24656782, Length=142, Percent_Identity=30.9859154929577, Blast_Score=85, Evalue=4e-17,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_CHRVO (Q7NYZ2)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_900800.1
- ProteinModelPortal:   Q7NYZ2
- SMR:   Q7NYZ2
- GeneID:   2550409
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1130
- NMPDR:   fig|243365.1.peg.1130
- HOGENOM:   HBG370191
- OMA:   RELWQKR
- ProtClustDB:   PRK09347
- BioCyc:   CVIO243365:CV_1130-MONOMER
- BRENDA:   3.5.4.16
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 23163; Mature: 23031

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFTDDHDHAACQRPLERVRPFDAAAFERAVADMLTASGIAIDPIHTGKTAQRVRELWQK
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHH
RLLDGYDTVPAEALGSGFADERRDMVVIRSLAVHGMCPHHLLPFRGVAHVAYLPGGRLHG
HHHCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHCCCCCCHHH
FGRIARMVDAISHRYTYQEWITHEIARTLVEHGEARGAACLIEAEQLCLLMGENRRGDER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCCHHH
VITQCYAGDFETDAEARNQFLRAIER
HHHHHHCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SFTDDHDHAACQRPLERVRPFDAAAFERAVADMLTASGIAIDPIHTGKTAQRVRELWQK
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHH
RLLDGYDTVPAEALGSGFADERRDMVVIRSLAVHGMCPHHLLPFRGVAHVAYLPGGRLHG
HHHCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHCCCCCCHHH
FGRIARMVDAISHRYTYQEWITHEIARTLVEHGEARGAACLIEAEQLCLLMGENRRGDER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCCHHH
VITQCYAGDFETDAEARNQFLRAIER
HHHHHHCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782