Click here to switch to the map view.

The map label for this gene is nifS [H]

Identifier: 34496549

GI number: 34496549

Start: 1139619

End: 1140836

Strand: Reverse

Name: nifS [H]

Synonym: CV_1094

Alternate gene names: 34496549

Gene position: 1140836-1139619 (Counterclockwise)

Preceding gene: 34496550

Following gene: 34496548

Centisome position: 24.01

GC content: 64.37

Gene sequence:

>1218_bases
ATGAGCCAGCCCAAGCTACCGATCTATCTGGATTACTCGGCCACCACCCCGGTGGACCCGCGCGTCGCCGAAAAGATGAT
TCCCTATCTGACCGGCAAGTTCGGCAACCCCGCCTCGCGCAGCCACAGCTTCGGCTGGGAAGCCGAGGAGGCCGTCGAAA
ACGCGCGCGCCGAAGTGGCCAAGCTGATCGGTGCCGATCCCAAGGAAATCGTCTGGACCTCCGGCGCCACCGAGTCCGAC
AACCTGGCGATCAAGGGCGCCGCCCAGTTCTACAAGAGCAAGGGCAAGCACATCATCACCGTCAAGACTGAGCATAAGGC
GGTGCTGGACACCTGTCGCGAGCTGGAGCGCCAAGGCTTCGAAGTGACCTATATGGACGTGCTGGAAAACGGCCTGCTCG
ACATCGAGGCGTTCAAGGCCGCCATCCGTCCGGACACCATCCTGGTGTCGGTGATGTACGTGAACAACGAGATCGGCGTG
ATCCAGGACATCCCCCTGATCGGCGAGATCTGCCGCGAGAAGGGCATCATCTTCCACGTCGACGCCACCCAGGCGCCGGG
CAAGGTGAAGATCGACCTGAACACGCTGAAAGTCGACCTGATGAGCCTGTCCGCGCACAAGGCCTACGGCCCGAAAGGCA
TCGGCGCGCTGTACGTGCGCCGCAAGCCGCGCGTGCGCCTGGAAGCCCAGATGCACGGCGGCGGCCACGAGCGCGGTTTC
CGCTCCGGCACGCTGGCCACCCACCAGATCGTCGGCATGGGCGAGGCCTTCCGCATCGCCCGCGAAGAGATGGACAGCGA
GTGCGAGCGCATCCGCATGCTGCGCGACCGCCTGTGGCACGGCATCCAGGACATGGAAGAGGTGTACATCAACGGCGACG
CCGACCAGCGCGTGCCGCACAACCTCAACGTCAGCTTCAACTTCGTCGAGGGCGAGAGCCTGATCATGGCCATCAAGGAC
CTGGCCGTGTCCAGCGGCTCCGCCTGCACCTCCGCCTCGCTGGAGCCGTCCTACGTGCTGCGCGCGCTGGGCCGCAACGA
CGAAATGGCCCACAGCTCCATCCGCTTCACGCTGGGCCGCTTCACCACCGAGGAAGAGATCGACTACGCGATCGCGCTGC
TGAAATCCAAGATCGGCAAGCTGCGCGAGCTGTCCCCGCTGTGGGAGATGTACAAGGACGGCGTGGACCTGAACAGCGTT
CAATGGGCCGCCCACTGA

Upstream 100 bases:

>100_bases
AGCAGCGTCGTGCAGGACAAGCGCGACAGCTGTAGCAGCTCCCGTGCAGCTGCCAGCAGCGCCCTGTGAGCGCACGAAAA
ATCTTGCGGAGATTAAATAA

Downstream 100 bases:

>100_bases
GCCGATAACAGATTACCGGCCGCGGCGCGCGCTGCGGCCTTGCAGGAGCGAAAGACATGGCATATAGCGAAAAAGTGTTG
GATCACTACGAAAATCCGCG

Product: pyridoxal-phosphate-dependent aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSQPKLPIYLDYSATTPVDPRVAEKMIPYLTGKFGNPASRSHSFGWEAEEAVENARAEVAKLIGADPKEIVWTSGATESD
NLAIKGAAQFYKSKGKHIITVKTEHKAVLDTCRELERQGFEVTYMDVLENGLLDIEAFKAAIRPDTILVSVMYVNNEIGV
IQDIPLIGEICREKGIIFHVDATQAPGKVKIDLNTLKVDLMSLSAHKAYGPKGIGALYVRRKPRVRLEAQMHGGGHERGF
RSGTLATHQIVGMGEAFRIAREEMDSECERIRMLRDRLWHGIQDMEEVYINGDADQRVPHNLNVSFNFVEGESLIMAIKD
LAVSSGSACTSASLEPSYVLRALGRNDEMAHSSIRFTLGRFTTEEEIDYAIALLKSKIGKLRELSPLWEMYKDGVDLNSV
QWAAH

Sequences:

>Translated_405_residues
MSQPKLPIYLDYSATTPVDPRVAEKMIPYLTGKFGNPASRSHSFGWEAEEAVENARAEVAKLIGADPKEIVWTSGATESD
NLAIKGAAQFYKSKGKHIITVKTEHKAVLDTCRELERQGFEVTYMDVLENGLLDIEAFKAAIRPDTILVSVMYVNNEIGV
IQDIPLIGEICREKGIIFHVDATQAPGKVKIDLNTLKVDLMSLSAHKAYGPKGIGALYVRRKPRVRLEAQMHGGGHERGF
RSGTLATHQIVGMGEAFRIAREEMDSECERIRMLRDRLWHGIQDMEEVYINGDADQRVPHNLNVSFNFVEGESLIMAIKD
LAVSSGSACTSASLEPSYVLRALGRNDEMAHSSIRFTLGRFTTEEEIDYAIALLKSKIGKLRELSPLWEMYKDGVDLNSV
QWAAH
>Mature_404_residues
SQPKLPIYLDYSATTPVDPRVAEKMIPYLTGKFGNPASRSHSFGWEAEEAVENARAEVAKLIGADPKEIVWTSGATESDN
LAIKGAAQFYKSKGKHIITVKTEHKAVLDTCRELERQGFEVTYMDVLENGLLDIEAFKAAIRPDTILVSVMYVNNEIGVI
QDIPLIGEICREKGIIFHVDATQAPGKVKIDLNTLKVDLMSLSAHKAYGPKGIGALYVRRKPRVRLEAQMHGGGHERGFR
SGTLATHQIVGMGEAFRIAREEMDSECERIRMLRDRLWHGIQDMEEVYINGDADQRVPHNLNVSFNFVEGESLIMAIKDL
AVSSGSACTSASLEPSYVLRALGRNDEMAHSSIRFTLGRFTTEEEIDYAIALLKSKIGKLRELSPLWEMYKDGVDLNSVQ
WAAH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=400, Percent_Identity=58, Blast_Score=513, Evalue=1e-145,
Organism=Homo sapiens, GI156713448, Length=420, Percent_Identity=32.3809523809524, Blast_Score=204, Evalue=1e-52,
Organism=Escherichia coli, GI48994898, Length=403, Percent_Identity=76.1786600496278, Blast_Score=652, Evalue=0.0,
Organism=Escherichia coli, GI1789175, Length=237, Percent_Identity=28.6919831223629, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787970, Length=270, Percent_Identity=26.2962962962963, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI25143064, Length=401, Percent_Identity=59.8503740648379, Blast_Score=507, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17533177, Length=324, Percent_Identity=32.0987654320988, Blast_Score=142, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6319831, Length=399, Percent_Identity=59.1478696741855, Blast_Score=513, Evalue=1e-146,
Organism=Drosophila melanogaster, GI20129463, Length=400, Percent_Identity=57.25, Blast_Score=491, Evalue=1e-139,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 45092; Mature: 44961

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPKLPIYLDYSATTPVDPRVAEKMIPYLTGKFGNPASRSHSFGWEAEEAVENARAEVA
CCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KLIGADPKEIVWTSGATESDNLAIKGAAQFYKSKGKHIITVKTEHKAVLDTCRELERQGF
HHHCCCCHHEEEECCCCCCCCEEEEHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCC
EVTYMDVLENGLLDIEAFKAAIRPDTILVSVMYVNNEIGVIQDIPLIGEICREKGIIFHV
EEEEHHHHHCCCEEHHHHHHHCCCCEEEEEEEEECCCCCCEECCHHHHHHHHHCCEEEEE
DATQAPGKVKIDLNTLKVDLMSLSAHKAYGPKGIGALYVRRKPRVRLEAQMHGGGHERGF
ECCCCCCEEEEEEEEEEEEEEHHHHHCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCC
RSGTLATHQIVGMGEAFRIAREEMDSECERIRMLRDRLWHGIQDMEEVYINGDADQRVPH
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEECCCCCCCCCC
NLNVSFNFVEGESLIMAIKDLAVSSGSACTSASLEPSYVLRALGRNDEMAHSSIRFTLGR
CCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHEEEECC
FTTEEEIDYAIALLKSKIGKLRELSPLWEMYKDGVDLNSVQWAAH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCC
>Mature Secondary Structure 
SQPKLPIYLDYSATTPVDPRVAEKMIPYLTGKFGNPASRSHSFGWEAEEAVENARAEVA
CCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KLIGADPKEIVWTSGATESDNLAIKGAAQFYKSKGKHIITVKTEHKAVLDTCRELERQGF
HHHCCCCHHEEEECCCCCCCCEEEEHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCC
EVTYMDVLENGLLDIEAFKAAIRPDTILVSVMYVNNEIGVIQDIPLIGEICREKGIIFHV
EEEEHHHHHCCCEEHHHHHHHCCCCEEEEEEEEECCCCCCEECCHHHHHHHHHCCEEEEE
DATQAPGKVKIDLNTLKVDLMSLSAHKAYGPKGIGALYVRRKPRVRLEAQMHGGGHERGF
ECCCCCCEEEEEEEEEEEEEEHHHHHCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCC
RSGTLATHQIVGMGEAFRIAREEMDSECERIRMLRDRLWHGIQDMEEVYINGDADQRVPH
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEECCCCCCCCCC
NLNVSFNFVEGESLIMAIKDLAVSSGSACTSASLEPSYVLRALGRNDEMAHSSIRFTLGR
CCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHEEEECC
FTTEEEIDYAIALLKSKIGKLRELSPLWEMYKDGVDLNSVQWAAH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA