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The map label for this gene is emrY [H]

Identifier: 34496533

GI number: 34496533

Start: 1125650

End: 1127164

Strand: Reverse

Name: emrY [H]

Synonym: CV_1078

Alternate gene names: 34496533

Gene position: 1127164-1125650 (Counterclockwise)

Preceding gene: 34496538

Following gene: 34496532

Centisome position: 23.72

GC content: 68.58

Gene sequence:

>1515_bases
ATGCCGCGCATGCATGCTTTTTCTCCTCAGCCCCATTGGAGCCGGCTGGCCGCGATCGCGCTGATCGCCTGGCTGGAATT
CCTGCAGAACGGCCTGCTCAATTTCGCGTCCTCGCAGGTGATGGGCGGGGTCGGCGCCGGACCGGAGGAGTTCAGCCAGG
CGGCGATGGCTTACGCGCTGTGCGCGGTGCTGGCGCTGTTCAATCATCAATGGTGCGTCGAGCGCTTCGGCTCGCGCCGC
CATCTGCAGGGTTCCATCCTGCTGTTCGCGCTGGGCGCGCTGATCGCCGGCGCCGCCGCATCTCCGGCAGCCTTCATCGC
CGGCCGCGCGGTGCAGGGCCTGGGCGGCGCCGCCTTCTTCACCGCCGCCCGTCTCGAAGTCGCGCAGCTGCCGCCCGCCA
AGCGCGGGCCGGGCCTGGTCGCCTTCGGCCAGGCGCTGCTGCTGGGCAGCGCGCTGGGGCCGCTGCTCGGCGGCGTCCTG
CTGCAGGCCTACGGTTGGCGCGCGCTGTGCTGGGGCATCCTTCCGCTGCTGCTGCCCGCCTGGTGGGCGGCTGGCCGCCT
GCCGCTGCGGCCGCCGGCGCATCGTCCCGCGCATTATCCGACCTGGACTTTCCTGCTGCTGCTGACCGCCGTCTTCCTGC
TGCAATGGCTGATCCAGCAAACGCCTTACCGGCTGTTCGCCCATCCGCAATGGCTGGCCGGCCTGGCGGCGGCGGCCGTC
GCCGCCTGGATGCTGTTCCGCGCCGCCCACCGCGGCCGGGCGTCCGACCGCCGCCGCTGGCGTCTGCTGCGCCGCCCAAG
CTATCGGCTGGGACTCGTCGGCTACAGCTGCTGCTACCTGCTGGTATCGGCCAACAGCTACATCCTGCCGATGCTGTCGG
TGCAGGCGCTGGGTTTCGACCTGCCCACCAGCGGCATGCTGCTGTCGGTCAGCTACCTGGCCGGCATCGCCTTCGCGCTG
CTGTACGCCAAGCTGCTGCTGAAAGGCAAGGCGCCCGGACTGAGAGAAACCATGGCCGGCGGCTGCCTGCTGCTGGCCGG
CTACGGCTGGCTGATGAGCGGCCTGAACGGCGAGGCGACGCTGGCCCGCGTCGCGGCCATCCTGCTGCTCAACGGCGGCT
TCATGTCGCTGTTCATCATGGCGGTGGCGCAGAGCGCCTTCCGCGGCATCGCCCCGGTAGCCTTCGGCCACGCCTACCAA
ACCAAGAACATCGTGCGCCAGGTGTCGGTCTCGGCCGGCGTGGCCTTGGCCAGCGTGTTCATGCAGGCGCGCAACGCGCT
GCATTACAGCCGTCTGTCCGAGCATTTTTCCTGGAATTCGCCACAGTTTCTCGACTCATTGCATCAATTACGACAATTCA
TGCCCGATTTGAGCGATACTCAACGCTTCGGAGTGCTGGCCGGCGAACTCGCCCGGCAAGCCACGCTGGTTTCCTGCCTG
GACTTCTTCCGCCTGGAAAGCTGGATAGGCCTGGCGCTGGCGGCCGGCCTGCTCGCGGCCAGAAAAAACGGCTGA

Upstream 100 bases:

>100_bases
TTCAGCGCGTCAATATCATTCACAGTAATATATCCAATGAAAAACATCGATTTTATTAAATCATAAGCGCTTTCTACACT
GGCGGCATCCCCACCCTCGG

Downstream 100 bases:

>100_bases
GCCGGCGCTCCGCGGCATCTCAGTTTCAGCGGGAAGAATCAAACCATGCGCAAAAGCGGCCAATCGGAGACCTTCAACAA
GATTGTCGAAGCGAGCCTGC

Product: multidrug resistence transmembrane protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MPRMHAFSPQPHWSRLAAIALIAWLEFLQNGLLNFASSQVMGGVGAGPEEFSQAAMAYALCAVLALFNHQWCVERFGSRR
HLQGSILLFALGALIAGAAASPAAFIAGRAVQGLGGAAFFTAARLEVAQLPPAKRGPGLVAFGQALLLGSALGPLLGGVL
LQAYGWRALCWGILPLLLPAWWAAGRLPLRPPAHRPAHYPTWTFLLLLTAVFLLQWLIQQTPYRLFAHPQWLAGLAAAAV
AAWMLFRAAHRGRASDRRRWRLLRRPSYRLGLVGYSCCYLLVSANSYILPMLSVQALGFDLPTSGMLLSVSYLAGIAFAL
LYAKLLLKGKAPGLRETMAGGCLLLAGYGWLMSGLNGEATLARVAAILLLNGGFMSLFIMAVAQSAFRGIAPVAFGHAYQ
TKNIVRQVSVSAGVALASVFMQARNALHYSRLSEHFSWNSPQFLDSLHQLRQFMPDLSDTQRFGVLAGELARQATLVSCL
DFFRLESWIGLALAAGLLAARKNG

Sequences:

>Translated_504_residues
MPRMHAFSPQPHWSRLAAIALIAWLEFLQNGLLNFASSQVMGGVGAGPEEFSQAAMAYALCAVLALFNHQWCVERFGSRR
HLQGSILLFALGALIAGAAASPAAFIAGRAVQGLGGAAFFTAARLEVAQLPPAKRGPGLVAFGQALLLGSALGPLLGGVL
LQAYGWRALCWGILPLLLPAWWAAGRLPLRPPAHRPAHYPTWTFLLLLTAVFLLQWLIQQTPYRLFAHPQWLAGLAAAAV
AAWMLFRAAHRGRASDRRRWRLLRRPSYRLGLVGYSCCYLLVSANSYILPMLSVQALGFDLPTSGMLLSVSYLAGIAFAL
LYAKLLLKGKAPGLRETMAGGCLLLAGYGWLMSGLNGEATLARVAAILLLNGGFMSLFIMAVAQSAFRGIAPVAFGHAYQ
TKNIVRQVSVSAGVALASVFMQARNALHYSRLSEHFSWNSPQFLDSLHQLRQFMPDLSDTQRFGVLAGELARQATLVSCL
DFFRLESWIGLALAAGLLAARKNG
>Mature_503_residues
PRMHAFSPQPHWSRLAAIALIAWLEFLQNGLLNFASSQVMGGVGAGPEEFSQAAMAYALCAVLALFNHQWCVERFGSRRH
LQGSILLFALGALIAGAAASPAAFIAGRAVQGLGGAAFFTAARLEVAQLPPAKRGPGLVAFGQALLLGSALGPLLGGVLL
QAYGWRALCWGILPLLLPAWWAAGRLPLRPPAHRPAHYPTWTFLLLLTAVFLLQWLIQQTPYRLFAHPQWLAGLAAAAVA
AWMLFRAAHRGRASDRRRWRLLRRPSYRLGLVGYSCCYLLVSANSYILPMLSVQALGFDLPTSGMLLSVSYLAGIAFALL
YAKLLLKGKAPGLRETMAGGCLLLAGYGWLMSGLNGEATLARVAAILLLNGGFMSLFIMAVAQSAFRGIAPVAFGHAYQT
KNIVRQVSVSAGVALASVFMQARNALHYSRLSEHFSWNSPQFLDSLHQLRQFMPDLSDTQRFGVLAGELARQATLVSCLD
FFRLESWIGLALAAGLLAARKNG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=428, Percent_Identity=21.0280373831776, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 54404; Mature: 54273

Theoretical pI: Translated: 10.97; Mature: 10.97

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRMHAFSPQPHWSRLAAIALIAWLEFLQNGLLNFASSQVMGGVGAGPEEFSQAAMAYAL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
CAVLALFNHQWCVERFGSRRHLQGSILLFALGALIAGAAASPAAFIAGRAVQGLGGAAFF
HHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHH
TAARLEVAQLPPAKRGPGLVAFGQALLLGSALGPLLGGVLLQAYGWRALCWGILPLLLPA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
WWAAGRLPLRPPAHRPAHYPTWTFLLLLTAVFLLQWLIQQTPYRLFAHPQWLAGLAAAAV
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHH
AAWMLFRAAHRGRASDRRRWRLLRRPSYRLGLVGYSCCYLLVSANSYILPMLSVQALGFD
HHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
LPTSGMLLSVSYLAGIAFALLYAKLLLKGKAPGLRETMAGGCLLLAGYGWLMSGLNGEAT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHCCCCCHHH
LARVAAILLLNGGFMSLFIMAVAQSAFRGIAPVAFGHAYQTKNIVRQVSVSAGVALASVF
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MQARNALHYSRLSEHFSWNSPQFLDSLHQLRQFMPDLSDTQRFGVLAGELARQATLVSCL
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
DFFRLESWIGLALAAGLLAARKNG
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PRMHAFSPQPHWSRLAAIALIAWLEFLQNGLLNFASSQVMGGVGAGPEEFSQAAMAYAL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH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HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]