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The map label for this gene is oppA [H]

Identifier: 34496255

GI number: 34496255

Start: 823682

End: 825280

Strand: Reverse

Name: oppA [H]

Synonym: CV_0800

Alternate gene names: 34496255

Gene position: 825280-823682 (Counterclockwise)

Preceding gene: 34496256

Following gene: 34496253

Centisome position: 17.37

GC content: 59.1

Gene sequence:

>1599_bases
ATGAAGGCCTCAGCATCCCTGTTGTTTCTTATTACGCTCATCGCCATGCCGGCACCGGCCGCCGTCATTCCAGCCGGCGC
TGACTTGGCGGAACGGCAAGAACTGATCCGCAGCAACGGATCGGAGCCTGCGACGCTGGATCCGCAACTGGCGGAAGCGC
TGCCCGACAATATGATCACCCACGACCTATTCGAAGGCCTGACCGCTATGGACAATGCCGGCCGCCTGCAAGCCGGTCTG
GCCGTATCATGGGAACGGAAAAATCCTGACACCTGGATATTCCATCTACGGCCGGATGCGCGCTGGAGCGATGGCGTCCC
CATTCGAGCTGCGGACGTCGTCTATAGCATCCAGCGACTGCTGGATCCGGCTTTGCATTCCCCTTACGCGAACGGCTTTG
GCGGCGTGCTGAAGAATGGCATGGCCGTAACGGCAGGGAAAATGCCGCTGGCTGAGGTCGGCGTGCGAGCAGTCGATGAC
TATACGGTGGAGCTGATGACTGCTTACCCGGTGGCGTTCTTGCCAGAATTGCTGGCCAATGCGCAATGGGGGCCGGTGCC
CAGGCATGCGATCCAGCGCTGGGGAAAAGACTGGGTGAACCCGGGGAAATTGGTTTCCAGCGGCGCCTTCCGGCTGGTCG
CGTGGACAGCGGGGCAAAGTATCGTCCTAGAAAAAAACAGGCAGTACTGGGATGCCGACTCGGTGGTATTGCAGCGAGTC
AACTATTTGCCGGTAGAGGATTTGGGCCAGGATGTCTCGCTTTATTTCTCCGGCAAAACCGATTTCGTTTTCCAACTCCC
CCCCGGCCAATTCGACAAGCTGAGACTCTCTCACCCCGCCGAGGTCAAAAACAGCCCGTTGCTGAGCTTGCGCTACTTCA
GCCTCAACCTGCGCGATCCTCTGCTGCGCGATGTGCGGGTGCGTAAGGCCTTGTCCATGGTGGTGGATCGCGACAAACTG
GCGGAATCCATCACGGCGGATGGGCAAAGCCCGGCATATAGCGTGATTCTGCAAGGCTTGAGAGGCGCGGCGCCCTCGTC
CTATGAATGGCAATCATGGTCCATGGCCCACAGGGTGGCAGCCGCCCGGCAGCTGCTGAAGGAGGCGGGCGTGGCGCCGC
ATCGCCAATTGCGGCTGATTTACAATACCAGCGACTACCAGAAAACCTTGAGCGAGTACTTGGCCAGCGAATGGAAAAGC
AAGCTGGGCCTCGATTCCCAGCTGGAATCGACCGACTATCCCAAGCTGATGCAAAAGCGGCATCAGGGACAATTCCAGAT
CGCTCGCAACGGTTGGACGGCAGACTACAACGACGCGATTTCCATGCTCGGCATCGTCCGCTGCGGCGACGCCGAGAATG
TTTCCGGCGGTTGCAACCAGACCGCGGAAGACTTGATCGAGCGCGCCAACCTGTCCCCCGACCCAGAACAGAGAAGCGCG
CTGTTGACAGAAGCCAATCGCCTCATCATGCAGGACTACACCATCATTCCCTTGCTGCAATACTCCGACTCCAGGCTGGT
CAAGCCCTATGTCGGCGGCTATGGCCAAAATCCGCTGGGACGCTATCGCAGCAAAGACATCTACATCATCCGCCATTGA

Upstream 100 bases:

>100_bases
TAACACCCATCCACTTTTCTGGACCGCGCAGCTTCCATTCCAATCGTGAGAAAGCATTGAAACTTTCCCTAACAATTTTC
GCCTGGCAGGAGTCCCTTTT

Downstream 100 bases:

>100_bases
CTGCATGCGGGCTTCGGCCGCTGACGATACCCCGGCCGCCCCAAACGCGGTTTACGAGGCGGCGAAGAAGCTGCTCGGCG
GCGCGCCCAGTTCGCGCTTG

Product: oligopeptide ABC transporter system, substrate-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MKASASLLFLITLIAMPAPAAVIPAGADLAERQELIRSNGSEPATLDPQLAEALPDNMITHDLFEGLTAMDNAGRLQAGL
AVSWERKNPDTWIFHLRPDARWSDGVPIRAADVVYSIQRLLDPALHSPYANGFGGVLKNGMAVTAGKMPLAEVGVRAVDD
YTVELMTAYPVAFLPELLANAQWGPVPRHAIQRWGKDWVNPGKLVSSGAFRLVAWTAGQSIVLEKNRQYWDADSVVLQRV
NYLPVEDLGQDVSLYFSGKTDFVFQLPPGQFDKLRLSHPAEVKNSPLLSLRYFSLNLRDPLLRDVRVRKALSMVVDRDKL
AESITADGQSPAYSVILQGLRGAAPSSYEWQSWSMAHRVAAARQLLKEAGVAPHRQLRLIYNTSDYQKTLSEYLASEWKS
KLGLDSQLESTDYPKLMQKRHQGQFQIARNGWTADYNDAISMLGIVRCGDAENVSGGCNQTAEDLIERANLSPDPEQRSA
LLTEANRLIMQDYTIIPLLQYSDSRLVKPYVGGYGQNPLGRYRSKDIYIIRH

Sequences:

>Translated_532_residues
MKASASLLFLITLIAMPAPAAVIPAGADLAERQELIRSNGSEPATLDPQLAEALPDNMITHDLFEGLTAMDNAGRLQAGL
AVSWERKNPDTWIFHLRPDARWSDGVPIRAADVVYSIQRLLDPALHSPYANGFGGVLKNGMAVTAGKMPLAEVGVRAVDD
YTVELMTAYPVAFLPELLANAQWGPVPRHAIQRWGKDWVNPGKLVSSGAFRLVAWTAGQSIVLEKNRQYWDADSVVLQRV
NYLPVEDLGQDVSLYFSGKTDFVFQLPPGQFDKLRLSHPAEVKNSPLLSLRYFSLNLRDPLLRDVRVRKALSMVVDRDKL
AESITADGQSPAYSVILQGLRGAAPSSYEWQSWSMAHRVAAARQLLKEAGVAPHRQLRLIYNTSDYQKTLSEYLASEWKS
KLGLDSQLESTDYPKLMQKRHQGQFQIARNGWTADYNDAISMLGIVRCGDAENVSGGCNQTAEDLIERANLSPDPEQRSA
LLTEANRLIMQDYTIIPLLQYSDSRLVKPYVGGYGQNPLGRYRSKDIYIIRH
>Mature_532_residues
MKASASLLFLITLIAMPAPAAVIPAGADLAERQELIRSNGSEPATLDPQLAEALPDNMITHDLFEGLTAMDNAGRLQAGL
AVSWERKNPDTWIFHLRPDARWSDGVPIRAADVVYSIQRLLDPALHSPYANGFGGVLKNGMAVTAGKMPLAEVGVRAVDD
YTVELMTAYPVAFLPELLANAQWGPVPRHAIQRWGKDWVNPGKLVSSGAFRLVAWTAGQSIVLEKNRQYWDADSVVLQRV
NYLPVEDLGQDVSLYFSGKTDFVFQLPPGQFDKLRLSHPAEVKNSPLLSLRYFSLNLRDPLLRDVRVRKALSMVVDRDKL
AESITADGQSPAYSVILQGLRGAAPSSYEWQSWSMAHRVAAARQLLKEAGVAPHRQLRLIYNTSDYQKTLSEYLASEWKS
KLGLDSQLESTDYPKLMQKRHQGQFQIARNGWTADYNDAISMLGIVRCGDAENVSGGCNQTAEDLIERANLSPDPEQRSA
LLTEANRLIMQDYTIIPLLQYSDSRLVKPYVGGYGQNPLGRYRSKDIYIIRH

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=520, Percent_Identity=38.8461538461538, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI87081878, Length=540, Percent_Identity=32.962962962963, Blast_Score=318, Evalue=6e-88,
Organism=Escherichia coli, GI1789397, Length=533, Percent_Identity=35.8348968105066, Blast_Score=309, Evalue=3e-85,
Organism=Escherichia coli, GI1787052, Length=501, Percent_Identity=23.5528942115768, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789966, Length=569, Percent_Identity=23.9015817223199, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1787762, Length=530, Percent_Identity=22.6415094339623, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1787551, Length=555, Percent_Identity=23.2432432432432, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59130; Mature: 59130

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASASLLFLITLIAMPAPAAVIPAGADLAERQELIRSNGSEPATLDPQLAEALPDNMIT
CCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHH
HDLFEGLTAMDNAGRLQAGLAVSWERKNPDTWIFHLRPDARWSDGVPIRAADVVYSIQRL
HHHHHHHHHCCCCCCEEECEEEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
LDPALHSPYANGFGGVLKNGMAVTAGKMPLAEVGVRAVDDYTVELMTAYPVAFLPELLAN
HHHHHCCCCCCCHHHHHHCCEEEEECCCCHHHHCCHHHCCCEEEHHHHCCHHHHHHHHCC
AQWGPVPRHAIQRWGKDWVNPGKLVSSGAFRLVAWTAGQSIVLEKNRQYWDADSVVLQRV
CCCCCCCHHHHHHHHHHHCCCCHHHCCCCEEEEEEECCCEEEEECCCCCCCHHHHHHHHH
NYLPVEDLGQDVSLYFSGKTDFVFQLPPGQFDKLRLSHPAEVKNSPLLSLRYFSLNLRDP
CCCCHHHCCCCEEEEECCCCCEEEECCCCCCCCEECCCCCCCCCCCEEEEEEEEECCCCH
LLRDVRVRKALSMVVDRDKLAESITADGQSPAYSVILQGLRGAAPSSYEWQSWSMAHRVA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
AARQLLKEAGVAPHRQLRLIYNTSDYQKTLSEYLASEWKSKLGLDSQLESTDYPKLMQKR
HHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
HQGQFQIARNGWTADYNDAISMLGIVRCGDAENVSGGCNQTAEDLIERANLSPDPEQRSA
CCCCEEEECCCCCCCHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
LLTEANRLIMQDYTIIPLLQYSDSRLVKPYVGGYGQNPLGRYRSKDIYIIRH
HHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCCCCHHHCCCCCEEEEEC
>Mature Secondary Structure
MKASASLLFLITLIAMPAPAAVIPAGADLAERQELIRSNGSEPATLDPQLAEALPDNMIT
CCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHH
HDLFEGLTAMDNAGRLQAGLAVSWERKNPDTWIFHLRPDARWSDGVPIRAADVVYSIQRL
HHHHHHHHHCCCCCCEEECEEEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
LDPALHSPYANGFGGVLKNGMAVTAGKMPLAEVGVRAVDDYTVELMTAYPVAFLPELLAN
HHHHHCCCCCCCHHHHHHCCEEEEECCCCHHHHCCHHHCCCEEEHHHHCCHHHHHHHHCC
AQWGPVPRHAIQRWGKDWVNPGKLVSSGAFRLVAWTAGQSIVLEKNRQYWDADSVVLQRV
CCCCCCCHHHHHHHHHHHCCCCHHHCCCCEEEEEEECCCEEEEECCCCCCCHHHHHHHHH
NYLPVEDLGQDVSLYFSGKTDFVFQLPPGQFDKLRLSHPAEVKNSPLLSLRYFSLNLRDP
CCCCHHHCCCCEEEEECCCCCEEEECCCCCCCCEECCCCCCCCCCCEEEEEEEEECCCCH
LLRDVRVRKALSMVVDRDKLAESITADGQSPAYSVILQGLRGAAPSSYEWQSWSMAHRVA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
AARQLLKEAGVAPHRQLRLIYNTSDYQKTLSEYLASEWKSKLGLDSQLESTDYPKLMQKR
HHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
HQGQFQIARNGWTADYNDAISMLGIVRCGDAENVSGGCNQTAEDLIERANLSPDPEQRSA
CCCCEEEECCCCCCCHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
LLTEANRLIMQDYTIIPLLQYSDSRLVKPYVGGYGQNPLGRYRSKDIYIIRH
HHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCCCCHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2821267; 3525163; 11677609; 8202710; 8747465; 10422831; 10438628 [H]