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The map label for this gene is asnB [H]

Identifier: 34496252

GI number: 34496252

Start: 819607

End: 821463

Strand: Reverse

Name: asnB [H]

Synonym: CV_0797

Alternate gene names: 34496252

Gene position: 821463-819607 (Counterclockwise)

Preceding gene: 34496253

Following gene: 34496251

Centisome position: 17.29

GC content: 68.34

Gene sequence:

>1857_bases
ATGTGTGGAATCGCCGGCTTCTTCTCGCGCCGCCCCGTCAATCCCGCCGTCCCGCAAGCCATGCTGGACGAGCTGCATCG
CCGCGGGCCGGACGATGCCAGCCGCCTGCTGCTGGATTCGGAGCTGAACCCGGCCGATGGCGGCGCCATCCACAACGCGA
TGCTGCATGCCCGGCTCGCCATCGTCGATCCGCGCCCGGTCGCCAACCAGCCGATGGCCAGCGATGACGGCCAGGTCTGG
ATCTGCTACAACGGCGAGGTCTACGACTGGGAAATCGGCAAGGCCGAGCTGGAAGCCGGCGGCGCCGTTTTCCGCACCCA
TTCCGACACCGAATTCATCCTGCGCGGCTACCAGGCCTGGGGCATCGAAGGCTTGCTGGAACGGCTGCGCGGCATGTTCG
CCTTCGCCATCCTGGACAAGCGCTGCGAAAAGGTCCATCTCGCCCGCGACCGCATGGGCGAAAAACCGCTGCTGTACTCG
CTGCAGGACGGCGACTTCGCCTACGGCTCGCTGGTGCGCGCGGTACTGCCCTTCCTGCCGGCCGACAAGCGGGCTTTCAG
CCCCGCGGCGATAGACGCCTATCTGGCCCACCGCTACATCCCGTCGCCGGCCACCGTGTTCAGCCATATCAACCGGCTGG
AGAACGGCTACCGGCTGGAATTCGACCTGAATACCCGCCAGTTGACCAAACAGCGCTACTGGCAGCCGAAGGCGGAGTCC
GGCGACTGGCTGCCGGAGCTGGATCGCGCGGTGGCGATGCGCACCGTGGCCGACCGTCCGCTGGGCGTGCTGCTGTCCGG
CGGCATCGACTCCACCGTGATCGCCAGCCGGCTCGCCATCCAGCAGCTGACCCAGTTCTCCAGCTTCACCGCCGCCTTTC
CCGGCTCCACGCTGGACGAATCCGGCGACGCCGCGGATTCCGCGCGGCGCATGGGCCTGCCCAATGTCAGGGTGGACATG
CCGGAAAACCTGGCCGCCGATTTCGAGCGCATCGTCGCCGACCTGGACCAGCCCTTCGCCGATCCGTCCAGCTTCCCCAC
CTGGTATCTGGCGCGCGAAGTCACCAAGCATGTCAAGGTGGTGCTGGTGGGCGACGGCGGCGACGAACTGCTGGCCGGCT
ACAAGCGCATGGGCAAGCATCTGCGCACCCGCTGGCGGCAAAACATCCGCCTGCCGCTGCCGATCAAGCCGCAGCTGGAC
AGCAAGCGTGGCAAGCTGGCCACCGAGCTGGCGATGGACTGGAAGAGCGCCTATTCGCTGCGCTTCTCCGGCTTCACGCC
GGGACAGCGCAGCTTTCTGCAAGGCGGGCGCAAGCTGGAGAAACTGTGCCACTGGCGCGCGCCCGACGACATCGGCCCGG
AGCCCGGCGACGGCTCGCCCGGCCTGCACCAGCTGCTGGAGCTGGACTTCGCCAACTACCTGCCCGATTACATCCTGCAG
AAGTCCGACCTGTGCACGATGGCGCATGGCCTGGAAGGCCGCGCGCCGCTGCTGGACCACGGCTTCTACCAGCGCCTGCT
GAGCACCTCCGCAGCCGAGCGCTACACCCGCCCGGCCAAGCTGATCTTCCGCCGCGCCATCCACCCGGCGCTGCCGACCG
ACTTCTTCCAGCGCAAGAAGCGCGGCTTCAATCCGCCCTTGTCCGGCTGGCTGAAAACCAGCCTGGCGCCGCGCTTCGAC
GGCCTGGGCTCGCGGCTGGCCGAGGCCAGCGGCGGCCAGCTGGACGCCGGAGCCGTGGACGCCTTCGCCCGCGCCTATCG
CGACGGCGCCGGCCATCTGGCCGAGCAGATGCTGCAGCTGCTGATCCTGGACGAGAGCCTGACGCAGCTGCGCCGGCTGT
GCCGCGAGCTGGGTTGA

Upstream 100 bases:

>100_bases
TGACACCGTCCGGCGGCGCGCCGGATACTGCGCGGCATTGAAACCCAAGCGGGACGCCGGGCACAGGCTGGCGTTCCGCG
CATTCGGAAGCGCAGTCATC

Downstream 100 bases:

>100_bases
TTATTGCCTTTTCGGCCCGGCCTGCGCCGGGCTAGCCTCTCTTTCCGACCGAGAAAATACATCCGGGACCGCATTGGAAT
CACGAATTGACGAAGTCATA

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MCGIAGFFSRRPVNPAVPQAMLDELHRRGPDDASRLLLDSELNPADGGAIHNAMLHARLAIVDPRPVANQPMASDDGQVW
ICYNGEVYDWEIGKAELEAGGAVFRTHSDTEFILRGYQAWGIEGLLERLRGMFAFAILDKRCEKVHLARDRMGEKPLLYS
LQDGDFAYGSLVRAVLPFLPADKRAFSPAAIDAYLAHRYIPSPATVFSHINRLENGYRLEFDLNTRQLTKQRYWQPKAES
GDWLPELDRAVAMRTVADRPLGVLLSGGIDSTVIASRLAIQQLTQFSSFTAAFPGSTLDESGDAADSARRMGLPNVRVDM
PENLAADFERIVADLDQPFADPSSFPTWYLAREVTKHVKVVLVGDGGDELLAGYKRMGKHLRTRWRQNIRLPLPIKPQLD
SKRGKLATELAMDWKSAYSLRFSGFTPGQRSFLQGGRKLEKLCHWRAPDDIGPEPGDGSPGLHQLLELDFANYLPDYILQ
KSDLCTMAHGLEGRAPLLDHGFYQRLLSTSAAERYTRPAKLIFRRAIHPALPTDFFQRKKRGFNPPLSGWLKTSLAPRFD
GLGSRLAEASGGQLDAGAVDAFARAYRDGAGHLAEQMLQLLILDESLTQLRRLCRELG

Sequences:

>Translated_618_residues
MCGIAGFFSRRPVNPAVPQAMLDELHRRGPDDASRLLLDSELNPADGGAIHNAMLHARLAIVDPRPVANQPMASDDGQVW
ICYNGEVYDWEIGKAELEAGGAVFRTHSDTEFILRGYQAWGIEGLLERLRGMFAFAILDKRCEKVHLARDRMGEKPLLYS
LQDGDFAYGSLVRAVLPFLPADKRAFSPAAIDAYLAHRYIPSPATVFSHINRLENGYRLEFDLNTRQLTKQRYWQPKAES
GDWLPELDRAVAMRTVADRPLGVLLSGGIDSTVIASRLAIQQLTQFSSFTAAFPGSTLDESGDAADSARRMGLPNVRVDM
PENLAADFERIVADLDQPFADPSSFPTWYLAREVTKHVKVVLVGDGGDELLAGYKRMGKHLRTRWRQNIRLPLPIKPQLD
SKRGKLATELAMDWKSAYSLRFSGFTPGQRSFLQGGRKLEKLCHWRAPDDIGPEPGDGSPGLHQLLELDFANYLPDYILQ
KSDLCTMAHGLEGRAPLLDHGFYQRLLSTSAAERYTRPAKLIFRRAIHPALPTDFFQRKKRGFNPPLSGWLKTSLAPRFD
GLGSRLAEASGGQLDAGAVDAFARAYRDGAGHLAEQMLQLLILDESLTQLRRLCRELG
>Mature_618_residues
MCGIAGFFSRRPVNPAVPQAMLDELHRRGPDDASRLLLDSELNPADGGAIHNAMLHARLAIVDPRPVANQPMASDDGQVW
ICYNGEVYDWEIGKAELEAGGAVFRTHSDTEFILRGYQAWGIEGLLERLRGMFAFAILDKRCEKVHLARDRMGEKPLLYS
LQDGDFAYGSLVRAVLPFLPADKRAFSPAAIDAYLAHRYIPSPATVFSHINRLENGYRLEFDLNTRQLTKQRYWQPKAES
GDWLPELDRAVAMRTVADRPLGVLLSGGIDSTVIASRLAIQQLTQFSSFTAAFPGSTLDESGDAADSARRMGLPNVRVDM
PENLAADFERIVADLDQPFADPSSFPTWYLAREVTKHVKVVLVGDGGDELLAGYKRMGKHLRTRWRQNIRLPLPIKPQLD
SKRGKLATELAMDWKSAYSLRFSGFTPGQRSFLQGGRKLEKLCHWRAPDDIGPEPGDGSPGLHQLLELDFANYLPDYILQ
KSDLCTMAHGLEGRAPLLDHGFYQRLLSTSAAERYTRPAKLIFRRAIHPALPTDFFQRKKRGFNPPLSGWLKTSLAPRFD
GLGSRLAEASGGQLDAGAVDAFARAYRDGAGHLAEQMLQLLILDESLTQLRRLCRELG

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=416, Percent_Identity=27.6442307692308, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI168229252, Length=416, Percent_Identity=27.6442307692308, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI168229250, Length=416, Percent_Identity=27.6442307692308, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI296010848, Length=358, Percent_Identity=27.9329608938547, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI296010852, Length=311, Percent_Identity=27.0096463022508, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI296010850, Length=311, Percent_Identity=27.0096463022508, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI1786889, Length=400, Percent_Identity=27, Blast_Score=92, Evalue=8e-20,
Organism=Drosophila melanogaster, GI45553209, Length=404, Percent_Identity=26.2376237623762, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 68831; Mature: 68831

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGFFSRRPVNPAVPQAMLDELHRRGPDDASRLLLDSELNPADGGAIHNAMLHARLA
CCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHEE
IVDPRPVANQPMASDDGQVWICYNGEVYDWEIGKAELEAGGAVFRTHSDTEFILRGYQAW
EECCCCCCCCCCCCCCCEEEEEECCEEEEEECCHHHHHCCCEEEEECCCHHHHHHHHHHC
GIEGLLERLRGMFAFAILDKRCEKVHLARDRMGEKPLLYSLQDGDFAYGSLVRAVLPFLP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCC
ADKRAFSPAAIDAYLAHRYIPSPATVFSHINRLENGYRLEFDLNTRQLTKQRYWQPKAES
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCCCC
GDWLPELDRAVAMRTVADRPLGVLLSGGIDSTVIASRLAIQQLTQFSSFTAAFPGSTLDE
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SGDAADSARRMGLPNVRVDMPENLAADFERIVADLDQPFADPSSFPTWYLAREVTKHVKV
CCCHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCEEE
VLVGDGGDELLAGYKRMGKHLRTRWRQNIRLPLPIKPQLDSKRGKLATELAMDWKSAYSL
EEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEE
RFSGFTPGQRSFLQGGRKLEKLCHWRAPDDIGPEPGDGSPGLHQLLELDFANYLPDYILQ
EECCCCCCHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHC
KSDLCTMAHGLEGRAPLLDHGFYQRLLSTSAAERYTRPAKLIFRRAIHPALPTDFFQRKK
CCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHH
RGFNPPLSGWLKTSLAPRFDGLGSRLAEASGGQLDAGAVDAFARAYRDGAGHLAEQMLQL
CCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
LILDESLTQLRRLCRELG
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCGIAGFFSRRPVNPAVPQAMLDELHRRGPDDASRLLLDSELNPADGGAIHNAMLHARLA
CCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHEE
IVDPRPVANQPMASDDGQVWICYNGEVYDWEIGKAELEAGGAVFRTHSDTEFILRGYQAW
EECCCCCCCCCCCCCCCEEEEEECCEEEEEECCHHHHHCCCEEEEECCCHHHHHHHHHHC
GIEGLLERLRGMFAFAILDKRCEKVHLARDRMGEKPLLYSLQDGDFAYGSLVRAVLPFLP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCC
ADKRAFSPAAIDAYLAHRYIPSPATVFSHINRLENGYRLEFDLNTRQLTKQRYWQPKAES
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCCCC
GDWLPELDRAVAMRTVADRPLGVLLSGGIDSTVIASRLAIQQLTQFSSFTAAFPGSTLDE
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SGDAADSARRMGLPNVRVDMPENLAADFERIVADLDQPFADPSSFPTWYLAREVTKHVKV
CCCHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCEEE
VLVGDGGDELLAGYKRMGKHLRTRWRQNIRLPLPIKPQLDSKRGKLATELAMDWKSAYSL
EEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEE
RFSGFTPGQRSFLQGGRKLEKLCHWRAPDDIGPEPGDGSPGLHQLLELDFANYLPDYILQ
EECCCCCCHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHC
KSDLCTMAHGLEGRAPLLDHGFYQRLLSTSAAERYTRPAKLIFRRAIHPALPTDFFQRKK
CCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHH
RGFNPPLSGWLKTSLAPRFDGLGSRLAEASGGQLDAGAVDAFARAYRDGAGHLAEQMLQL
CCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
LILDESLTQLRRLCRELG
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]