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The map label for this gene is rstB [H]

Identifier: 34496215

GI number: 34496215

Start: 779440

End: 780792

Strand: Reverse

Name: rstB [H]

Synonym: CV_0760

Alternate gene names: 34496215

Gene position: 780792-779440 (Counterclockwise)

Preceding gene: 34496216

Following gene: 34496214

Centisome position: 16.43

GC content: 65.93

Gene sequence:

>1353_bases
ATGCCGTCCAAGAACCTGCGCGGCTGGCTGCGGCCGTCGCTGGGTACGCTGTTCGCCCGCTACTTCCTGATCACCATGCT
GATCCAGCTGTTGATCATCATCGGTTTCGGCTTTCTGGTGACCAAGCTGTACGAAAACAGCGACCAGGCCGGCAAGACCC
AGTTCATGCGCGGCACCGTGGCGCTGATCCAGGACAAGCTGAGGGGGCAGCCGCCCCAGCGCTGGGACGCCGAGCTGGCC
TCCATCGCCAGCCGCTTCAGCTATCCGATCCGCCTGATCGACCAGCCGCCGGCCGAGCTGGACGGCCAGGCCCAAGCCCT
GCTGCGCCGCGGCCAATCCTACCTGGACGCCGACAGCCAGGTGCTTTACGCGCCGATGCCCTCCGGGGGCAGGCTGCTGG
CGCTCGGCCCGCTGGACATGCAATCCAGCGACAGCAACTGGATCAGCGAAGACATCGAAGTGCTGCTGATCTGGATGCTG
CTGTCCGGCGCCACGCTGGGCACCCTGCTTTATGTCATGCTGCGACCGCTGTGGACCGACCTGCTGGAAGTGCGCCGCAC
CGCCGAGATGTTCGCCGAGGGCGACTTCAGCGCCCGCGCCCGCTCCGCCAGGAGCCGGCTGTTCGCGCCGCTGCCGCTGG
CCTTCAACAGCATGGCCTCGCGGCTGGAGCGCCAGCTGGAAACGCGCCAGGCGTTGTCGCACGCCATCGCCCACGAGATC
CGCACGCCCATCGCCCGTCTGCGCTTCGGCCTGACCATGCTGGAGGAGGAAGAGGATCCGATCGAGTGGAAGCGTTATCG
CGACGGCATGGAACGTGACATGGTGGAGCTGGAGGAGCTGATCAGCACCAGCATGGAGTTCGCCAAGCTGCGCCGCAGCG
AGGTGCCGCTGCACTTGGAGACGGTGGATCTGTTCGACTGGTTTGACGACCTGATCGACCTGGTCACCCCGCTGAAGCCG
CCCAACCTGACGTTGACGCTGGAATGCCCGCGCGAGGACGGCGCCTTCGACCGCAAGCTGATGTACATCGCCACCCGCAA
CCTGCTGCTGAACGCGTTCAAATATGCGCAATCGCAAGTGAGGATGACGGTCTACCATCAGGGCGCCCAGTTGATCATCG
AAGTGGATGACGACGGCTGCGGCATCCCGGAGGAAGACCGCGACAAGGTGTTCGAACCCTTTTTGCGGCTGGACCGCAGC
CGCGACCGCGCCACCGGCGGCCACGGCCTGGGCCTGTCCTTCGTGCGCCTGATCGCCGAACACCACCACGGCCATGCCAG
CGCCGGGGCCAGCACGCTCGGCGGCGCCCGCTTCCGCATGATCATTGCGCAACCTTCCCCTTGCGGGCAATAA

Upstream 100 bases:

>100_bases
GCTGCGCCGCAAACTGGGCGACAACCCCGATGCGCCGGCGCGGATCAAGACCGTGCGCGGCAAGGGCTATTTGTTCTCGC
GCAGCGACTGGGAATAGCCG

Downstream 100 bases:

>100_bases
GTCACTCATTGTCACAAAGCATCGACAGGAAGCCCACAGACCGCTTGCGCTTGATTCCGGATAATTCCGATTATCGAAAA
TCAGGAATATGCAGAATGGT

Product: two-component sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MPSKNLRGWLRPSLGTLFARYFLITMLIQLLIIIGFGFLVTKLYENSDQAGKTQFMRGTVALIQDKLRGQPPQRWDAELA
SIASRFSYPIRLIDQPPAELDGQAQALLRRGQSYLDADSQVLYAPMPSGGRLLALGPLDMQSSDSNWISEDIEVLLIWML
LSGATLGTLLYVMLRPLWTDLLEVRRTAEMFAEGDFSARARSARSRLFAPLPLAFNSMASRLERQLETRQALSHAIAHEI
RTPIARLRFGLTMLEEEEDPIEWKRYRDGMERDMVELEELISTSMEFAKLRRSEVPLHLETVDLFDWFDDLIDLVTPLKP
PNLTLTLECPREDGAFDRKLMYIATRNLLLNAFKYAQSQVRMTVYHQGAQLIIEVDDDGCGIPEEDRDKVFEPFLRLDRS
RDRATGGHGLGLSFVRLIAEHHHGHASAGASTLGGARFRMIIAQPSPCGQ

Sequences:

>Translated_450_residues
MPSKNLRGWLRPSLGTLFARYFLITMLIQLLIIIGFGFLVTKLYENSDQAGKTQFMRGTVALIQDKLRGQPPQRWDAELA
SIASRFSYPIRLIDQPPAELDGQAQALLRRGQSYLDADSQVLYAPMPSGGRLLALGPLDMQSSDSNWISEDIEVLLIWML
LSGATLGTLLYVMLRPLWTDLLEVRRTAEMFAEGDFSARARSARSRLFAPLPLAFNSMASRLERQLETRQALSHAIAHEI
RTPIARLRFGLTMLEEEEDPIEWKRYRDGMERDMVELEELISTSMEFAKLRRSEVPLHLETVDLFDWFDDLIDLVTPLKP
PNLTLTLECPREDGAFDRKLMYIATRNLLLNAFKYAQSQVRMTVYHQGAQLIIEVDDDGCGIPEEDRDKVFEPFLRLDRS
RDRATGGHGLGLSFVRLIAEHHHGHASAGASTLGGARFRMIIAQPSPCGQ
>Mature_449_residues
PSKNLRGWLRPSLGTLFARYFLITMLIQLLIIIGFGFLVTKLYENSDQAGKTQFMRGTVALIQDKLRGQPPQRWDAELAS
IASRFSYPIRLIDQPPAELDGQAQALLRRGQSYLDADSQVLYAPMPSGGRLLALGPLDMQSSDSNWISEDIEVLLIWMLL
SGATLGTLLYVMLRPLWTDLLEVRRTAEMFAEGDFSARARSARSRLFAPLPLAFNSMASRLERQLETRQALSHAIAHEIR
TPIARLRFGLTMLEEEEDPIEWKRYRDGMERDMVELEELISTSMEFAKLRRSEVPLHLETVDLFDWFDDLIDLVTPLKPP
NLTLTLECPREDGAFDRKLMYIATRNLLLNAFKYAQSQVRMTVYHQGAQLIIEVDDDGCGIPEEDRDKVFEPFLRLDRSR
DRATGGHGLGLSFVRLIAEHHHGHASAGASTLGGARFRMIIAQPSPCGQ

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=428, Percent_Identity=30.3738317757009, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1790346, Length=268, Percent_Identity=29.1044776119403, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789808, Length=241, Percent_Identity=25.3112033195021, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI48994928, Length=238, Percent_Identity=29.4117647058824, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788393, Length=263, Percent_Identity=26.9961977186312, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788279, Length=231, Percent_Identity=29.004329004329, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786912, Length=234, Percent_Identity=29.0598290598291, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786600, Length=231, Percent_Identity=28.1385281385281, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788549, Length=270, Percent_Identity=24.0740740740741, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790551, Length=264, Percent_Identity=28.4090909090909, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1787374, Length=312, Percent_Identity=27.8846153846154, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1786783, Length=246, Percent_Identity=26.4227642276423, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790861, Length=325, Percent_Identity=25.5384615384615, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082128, Length=193, Percent_Identity=25.9067357512953, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1789149, Length=215, Percent_Identity=26.046511627907, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50974; Mature: 50843

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSKNLRGWLRPSLGTLFARYFLITMLIQLLIIIGFGFLVTKLYENSDQAGKTQFMRGTV
CCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
ALIQDKLRGQPPQRWDAELASIASRFSYPIRLIDQPPAELDGQAQALLRRGQSYLDADSQ
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHCCHHHCCCCCC
VLYAPMPSGGRLLALGPLDMQSSDSNWISEDIEVLLIWMLLSGATLGTLLYVMLRPLWTD
EEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LLEVRRTAEMFAEGDFSARARSARSRLFAPLPLAFNSMASRLERQLETRQALSHAIAHEI
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTPIARLRFGLTMLEEEEDPIEWKRYRDGMERDMVELEELISTSMEFAKLRRSEVPLHLE
HHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
TVDLFDWFDDLIDLVTPLKPPNLTLTLECPREDGAFDRKLMYIATRNLLLNAFKYAQSQV
HHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RMTVYHQGAQLIIEVDDDGCGIPEEDRDKVFEPFLRLDRSRDRATGGHGLGLSFVRLIAE
HHHEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
HHHGHASAGASTLGGARFRMIIAQPSPCGQ
HCCCCCCCCCHHCCCCEEEEEEECCCCCCC
>Mature Secondary Structure 
PSKNLRGWLRPSLGTLFARYFLITMLIQLLIIIGFGFLVTKLYENSDQAGKTQFMRGTV
CCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
ALIQDKLRGQPPQRWDAELASIASRFSYPIRLIDQPPAELDGQAQALLRRGQSYLDADSQ
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHCCHHHCCCCCC
VLYAPMPSGGRLLALGPLDMQSSDSNWISEDIEVLLIWMLLSGATLGTLLYVMLRPLWTD
EEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LLEVRRTAEMFAEGDFSARARSARSRLFAPLPLAFNSMASRLERQLETRQALSHAIAHEI
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTPIARLRFGLTMLEEEEDPIEWKRYRDGMERDMVELEELISTSMEFAKLRRSEVPLHLE
HHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
TVDLFDWFDDLIDLVTPLKPPNLTLTLECPREDGAFDRKLMYIATRNLLLNAFKYAQSQV
HHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RMTVYHQGAQLIIEVDDDGCGIPEEDRDKVFEPFLRLDRSRDRATGGHGLGLSFVRLIAE
HHHEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
HHHGHASAGASTLGGARFRMIIAQPSPCGQ
HCCCCCCCCCHHCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]