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The map label for this gene is hemF

Identifier: 34496212

GI number: 34496212

Start: 776635

End: 777543

Strand: Reverse

Name: hemF

Synonym: CV_0757

Alternate gene names: 34496212

Gene position: 777543-776635 (Counterclockwise)

Preceding gene: 34496213

Following gene: 34496207

Centisome position: 16.37

GC content: 68.21

Gene sequence:

>909_bases
ATGAGCCACCCGCACAGCAACGCCGTCAAATCCTTTCTGCTGGACCTGCAGGACCGCATTTGCGCGGCGCTGGAGCAGGC
TGACGGCAAGGCGCAGTTCGCCGAAGACGCCTGGAGCCGCGAGGCCGGCGGCGGCGGGCGCAGCCGGGTGCTGACCGGCG
GCGAGGTGTTCGAGCAGGCCGGCGTCAACTTCTCCCATGTCCACGGCGACGCGCTGCCGCCCTCCGCCAGCGCCCACCGG
CCGGAGCTGGCCGGCCGCCGCTTCGAGGCGATGGGCGTGTCGCTGGTGATCCACCCGTCCAATCCGCACGTGCCCACCAG
CCACGCCAACGTGCGTTTCTTCATCGCGGAAAAGGACGGCGAGGCGCCGGTATGGTGGTTCGGCGGCGGTTTCGACCTGA
CGCCGTTCTACCCGCAGGAAGAGGACGCCGTGCACTGGCACACGGTGGCCCGCGACCTGTGCGCGCCGTTCGGCGGCGAC
GTCTATCCCCGCTACAAGAAATGGTGCGACGAATATTTCCACCTGAAGCACCGCAATGAGGCCCGCGGCATCGGCGGCCT
GTTCTTCGACGACCTCAACGAATGGGGCTTCGACAAGAGCTTCGCCTTCACCCGCGCGGTCGGCGACGGCTACCTGGACG
CCTATCTGCCCATCGTCGCGCGGCGCAAGGAACAAGCCTGGGGCGACCGCGAGCGCCAGTTCCAGCTGTACCGGCGCGGC
CGCTACGTCGAATTCAACCTGGTCTGGGACCGTGGCACGCTGTTCGGCCTGCAGTCAGGCGGCCGCACCGAATCGATACT
GATGTCGATGCCGCCGCTGGTGCGCTGGGAATACGGCTACCAGCCGGAACCGGGCAGCCCGGAAGCCAGGCTGTACACCG
ACTTTCTGCCGCCGCGCGACTGGGTGTGA

Upstream 100 bases:

>100_bases
GACAGCCCGCCCAGCGGGCTGTTTCGCATGCAGCCGGCCCGGCATCAGGCATGGCCCGCTCTGTTACAATCACAGCCTGT
TTCTTCATCAGGACAACGCC

Downstream 100 bases:

>100_bases
TCAGGCCGGGGGGGCCGGCTGCTTGGGAATCTGCCGCAGCCAGCGCAGCGTCCTGTCCTTCTCCTCGGCCGAGCACGCTC
GGCGCGTGCTGGAAATCGTG

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MSHPHSNAVKSFLLDLQDRICAALEQADGKAQFAEDAWSREAGGGGRSRVLTGGEVFEQAGVNFSHVHGDALPPSASAHR
PELAGRRFEAMGVSLVIHPSNPHVPTSHANVRFFIAEKDGEAPVWWFGGGFDLTPFYPQEEDAVHWHTVARDLCAPFGGD
VYPRYKKWCDEYFHLKHRNEARGIGGLFFDDLNEWGFDKSFAFTRAVGDGYLDAYLPIVARRKEQAWGDRERQFQLYRRG
RYVEFNLVWDRGTLFGLQSGGRTESILMSMPPLVRWEYGYQPEPGSPEARLYTDFLPPRDWV

Sequences:

>Translated_302_residues
MSHPHSNAVKSFLLDLQDRICAALEQADGKAQFAEDAWSREAGGGGRSRVLTGGEVFEQAGVNFSHVHGDALPPSASAHR
PELAGRRFEAMGVSLVIHPSNPHVPTSHANVRFFIAEKDGEAPVWWFGGGFDLTPFYPQEEDAVHWHTVARDLCAPFGGD
VYPRYKKWCDEYFHLKHRNEARGIGGLFFDDLNEWGFDKSFAFTRAVGDGYLDAYLPIVARRKEQAWGDRERQFQLYRRG
RYVEFNLVWDRGTLFGLQSGGRTESILMSMPPLVRWEYGYQPEPGSPEARLYTDFLPPRDWV
>Mature_301_residues
SHPHSNAVKSFLLDLQDRICAALEQADGKAQFAEDAWSREAGGGGRSRVLTGGEVFEQAGVNFSHVHGDALPPSASAHRP
ELAGRRFEAMGVSLVIHPSNPHVPTSHANVRFFIAEKDGEAPVWWFGGGFDLTPFYPQEEDAVHWHTVARDLCAPFGGDV
YPRYKKWCDEYFHLKHRNEARGIGGLFFDDLNEWGFDKSFAFTRAVGDGYLDAYLPIVARRKEQAWGDRERQFQLYRRGR
YVEFNLVWDRGTLFGLQSGGRTESILMSMPPLVRWEYGYQPEPGSPEARLYTDFLPPRDWV

Specific function: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III

COG id: COG0408

COG function: function code H; Coproporphyrinogen III oxidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerobic coproporphyrinogen-III oxidase family

Homologues:

Organism=Homo sapiens, GI41393599, Length=299, Percent_Identity=47.1571906354515, Blast_Score=276, Evalue=1e-74,
Organism=Escherichia coli, GI1788777, Length=299, Percent_Identity=77.9264214046823, Blast_Score=477, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6320249, Length=318, Percent_Identity=44.0251572327044, Blast_Score=259, Evalue=4e-70,
Organism=Drosophila melanogaster, GI17864388, Length=300, Percent_Identity=48, Blast_Score=280, Evalue=6e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM6_CHRVO (Q7P012)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_900427.1
- ProteinModelPortal:   Q7P012
- SMR:   Q7P012
- GeneID:   2551035
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_0757
- NMPDR:   fig|243365.1.peg.757
- HOGENOM:   HBG631180
- OMA:   HMNTRFV
- PhylomeDB:   Q7P012
- ProtClustDB:   PRK05330
- BioCyc:   CVIO243365:CV_0757-MONOMER
- BRENDA:   1.3.3.3
- GO:   GO:0005737
- HAMAP:   MF_00333
- InterPro:   IPR001260
- InterPro:   IPR018375
- Gene3D:   G3DSA:3.40.1500.10
- PANTHER:   PTHR10755
- PIRSF:   PIRSF000166
- PRINTS:   PR00073

Pfam domain/function: PF01218 Coprogen_oxidas; SSF102886 Coprogen_oxidas

EC number: =1.3.3.3

Molecular weight: Translated: 34267; Mature: 34135

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS01021 COPROGEN_OXIDASE

Important sites: ACT_SITE 108-108 BINDING 94-94

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHPHSNAVKSFLLDLQDRICAALEQADGKAQFAEDAWSREAGGGGRSRVLTGGEVFEQA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEECHHHHHHH
GVNFSHVHGDALPPSASAHRPELAGRRFEAMGVSLVIHPSNPHVPTSHANVRFFIAEKDG
CCCEEEECCCCCCCCCCCCCCHHHCCEEEECCEEEEEECCCCCCCCCCCCEEEEEEECCC
EAPVWWFGGGFDLTPFYPQEEDAVHWHTVARDLCAPFGGDVYPRYKKWCDEYFHLKHRNE
CCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
ARGIGGLFFDDLNEWGFDKSFAFTRAVGDGYLDAYLPIVARRKEQAWGDRERQFQLYRRG
CCCCCCHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
RYVEFNLVWDRGTLFGLQSGGRTESILMSMPPLVRWEYGYQPEPGSPEARLYTDFLPPRD
CEEEEEEEECCCEEEEECCCCCCHHHHCCCCCCEEEECCCCCCCCCCCCEEEECCCCCCC
WV
CC
>Mature Secondary Structure 
SHPHSNAVKSFLLDLQDRICAALEQADGKAQFAEDAWSREAGGGGRSRVLTGGEVFEQA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEECHHHHHHH
GVNFSHVHGDALPPSASAHRPELAGRRFEAMGVSLVIHPSNPHVPTSHANVRFFIAEKDG
CCCEEEECCCCCCCCCCCCCCHHHCCEEEECCEEEEEECCCCCCCCCCCCEEEEEEECCC
EAPVWWFGGGFDLTPFYPQEEDAVHWHTVARDLCAPFGGDVYPRYKKWCDEYFHLKHRNE
CCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
ARGIGGLFFDDLNEWGFDKSFAFTRAVGDGYLDAYLPIVARRKEQAWGDRERQFQLYRRG
CCCCCCHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
RYVEFNLVWDRGTLFGLQSGGRTESILMSMPPLVRWEYGYQPEPGSPEARLYTDFLPPRD
CEEEEEEEECCCEEEEECCCCCCHHHHCCCCCCEEEECCCCCCCCCCCCEEEECCCCCCC
WV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782