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The map label for this gene is yafC [H]

Identifier: 34496154

GI number: 34496154

Start: 725451

End: 726326

Strand: Reverse

Name: yafC [H]

Synonym: CV_0699

Alternate gene names: 34496154

Gene position: 726326-725451 (Counterclockwise)

Preceding gene: 34496162

Following gene: 34496153

Centisome position: 15.29

GC content: 70.55

Gene sequence:

>876_bases
ATGCTAAGCACGGAAAGCCTGATTGCCTTCACCACGATCATGGATTGCGGCTCCTTCACCGCCGCCGCCGACAAGCTGGG
CGTCACCGCCTCCAATGTCAGCCGCAGCCTGGCGCAGCTGGAAAAACAGCTGGGCGTGCGGCTGCTGACCCGCACCACGC
GGCGGCTGGACCTGACCGCGGAAGGCGCGTGGCTGCTGGAGCGCGCCCGCGTGATTCTGGAAAACCTGAACGATGCCGAG
CAGCAGCTGCAGGCCGCCGCCGCGCGGCCGTCCGGCCTGGTGCGCGTCAACGCCGCCATCCCGGTGCTGGACCACCTGCT
GGCGCCGCATCTGGCCGGCTTCCAGGCCCGCTACCCGGACATCCGGCTGGAGCTGGCCGGGGCAGAGGCGGTGGTGGACC
TGATCGAGGAACGCGCGGACCTGGCGATACGCGTCGGACCGCTGGCCGACTCCACGCTGAACGCGCGGCTGCTGTGCGAA
AGCCCGCTGCGCATCGTCGCCGCGCCGGATTATCTGCGCCGGCACGGCCGGCCCCGGACGGCGGCGGACCTGCAGGCGCA
CAGGCAGCTCGGCTTCACCAGCCCGGCCTCGCTGAATCTGTGGCCGCTGGGCGCCAACGGTTTGAAAGTGAACCCGTCGC
CGGCCGCCTCCAGCGGCGCCACGCTGCGCGCGCTGGCGCTGCACGGCTGCGGCATCGCCTGCCTGGCTGACTTTCTGGTG
GGGCCGGACATCGCGGCCGGGAGGCTGGAGGCAGTGCGCCTGCCGGAACAACGGGACTGGAAACAGCCGATCTGGGCTGT
ATTCTACAAACAGGGCCGACCGCTGCCGCGCATCGCCTGCCTGGTGGACTACCTGGCGGACGCGCTGGCTGAATGA

Upstream 100 bases:

>100_bases
ATGATTGCCTCCATTGAGAAATCAGGACGCCGATTGTCCGCGCTTGCCGCGCCCGGAAAAATGGGCTGTACTGACAAGGT
CTTTTGCGAAAAAATTGATA

Downstream 100 bases:

>100_bases
GCTAAAACTGACTATCCGAAGCAGTCCCAAAACATTACATTGGCGGTAATGTCCCAGGAGTACGCTGCCGGCATGAAACC
GTTGATCCTGTTGTTCGCCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MLSTESLIAFTTIMDCGSFTAAADKLGVTASNVSRSLAQLEKQLGVRLLTRTTRRLDLTAEGAWLLERARVILENLNDAE
QQLQAAAARPSGLVRVNAAIPVLDHLLAPHLAGFQARYPDIRLELAGAEAVVDLIEERADLAIRVGPLADSTLNARLLCE
SPLRIVAAPDYLRRHGRPRTAADLQAHRQLGFTSPASLNLWPLGANGLKVNPSPAASSGATLRALALHGCGIACLADFLV
GPDIAAGRLEAVRLPEQRDWKQPIWAVFYKQGRPLPRIACLVDYLADALAE

Sequences:

>Translated_291_residues
MLSTESLIAFTTIMDCGSFTAAADKLGVTASNVSRSLAQLEKQLGVRLLTRTTRRLDLTAEGAWLLERARVILENLNDAE
QQLQAAAARPSGLVRVNAAIPVLDHLLAPHLAGFQARYPDIRLELAGAEAVVDLIEERADLAIRVGPLADSTLNARLLCE
SPLRIVAAPDYLRRHGRPRTAADLQAHRQLGFTSPASLNLWPLGANGLKVNPSPAASSGATLRALALHGCGIACLADFLV
GPDIAAGRLEAVRLPEQRDWKQPIWAVFYKQGRPLPRIACLVDYLADALAE
>Mature_291_residues
MLSTESLIAFTTIMDCGSFTAAADKLGVTASNVSRSLAQLEKQLGVRLLTRTTRRLDLTAEGAWLLERARVILENLNDAE
QQLQAAAARPSGLVRVNAAIPVLDHLLAPHLAGFQARYPDIRLELAGAEAVVDLIEERADLAIRVGPLADSTLNARLLCE
SPLRIVAAPDYLRRHGRPRTAADLQAHRQLGFTSPASLNLWPLGANGLKVNPSPAASSGATLRALALHGCGIACLADFLV
GPDIAAGRLEAVRLPEQRDWKQPIWAVFYKQGRPLPRIACLVDYLADALAE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=41.3194444444444, Blast_Score=232, Evalue=2e-62,
Organism=Escherichia coli, GI1789639, Length=287, Percent_Identity=29.2682926829268, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=33.2191780821918, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI87081978, Length=292, Percent_Identity=30.8219178082192, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1787128, Length=275, Percent_Identity=28.3636363636364, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1789440, Length=287, Percent_Identity=27.5261324041812, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787589, Length=207, Percent_Identity=31.8840579710145, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789173, Length=234, Percent_Identity=30.7692307692308, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1788706, Length=242, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788481, Length=293, Percent_Identity=25.5972696245734, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082132, Length=172, Percent_Identity=33.7209302325581, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788748, Length=178, Percent_Identity=33.7078651685393, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786448, Length=250, Percent_Identity=27.6, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1789492, Length=262, Percent_Identity=30.5343511450382, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693105, Length=170, Percent_Identity=26.4705882352941, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI157672245, Length=165, Percent_Identity=29.6969696969697, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790262, Length=174, Percent_Identity=27.0114942528736, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787806, Length=284, Percent_Identity=26.056338028169, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31321; Mature: 31321

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSTESLIAFTTIMDCGSFTAAADKLGVTASNVSRSLAQLEKQLGVRLLTRTTRRLDLTA
CCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
EGAWLLERARVILENLNDAEQQLQAAAARPSGLVRVNAAIPVLDHLLAPHLAGFQARYPD
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCC
IRLELAGAEAVVDLIEERADLAIRVGPLADSTLNARLLCESPLRIVAAPDYLRRHGRPRT
EEEEECCHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEECCCCEEEECCHHHHHCCCCCC
AADLQAHRQLGFTSPASLNLWPLGANGLKVNPSPAASSGATLRALALHGCGIACLADFLV
HHHHHHHHHCCCCCCCCCEEEEECCCCEEECCCCCCCCCCCEEHHHHHCCCHHHHHHHHH
GPDIAAGRLEAVRLPEQRDWKQPIWAVFYKQGRPLPRIACLVDYLADALAE
CCCCCCCCHHEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSTESLIAFTTIMDCGSFTAAADKLGVTASNVSRSLAQLEKQLGVRLLTRTTRRLDLTA
CCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
EGAWLLERARVILENLNDAEQQLQAAAARPSGLVRVNAAIPVLDHLLAPHLAGFQARYPD
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCC
IRLELAGAEAVVDLIEERADLAIRVGPLADSTLNARLLCESPLRIVAAPDYLRRHGRPRT
EEEEECCHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEECCCCEEEECCHHHHHCCCCCC
AADLQAHRQLGFTSPASLNLWPLGANGLKVNPSPAASSGATLRALALHGCGIACLADFLV
HHHHHHHHHCCCCCCCCCEEEEECCCCEEECCCCCCCCCCCEEHHHHHCCCHHHHHHHHH
GPDIAAGRLEAVRLPEQRDWKQPIWAVFYKQGRPLPRIACLVDYLADALAE
CCCCCCCCHHEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]