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The map label for this gene is lcrF [H]

Identifier: 34496134

GI number: 34496134

Start: 706404

End: 707291

Strand: Reverse

Name: lcrF [H]

Synonym: CV_0679

Alternate gene names: 34496134

Gene position: 707291-706404 (Counterclockwise)

Preceding gene: 34496135

Following gene: 34496133

Centisome position: 14.89

GC content: 53.72

Gene sequence:

>888_bases
ATGACACTCGAAGTAGTACCGGCGGCGCTTTATTCGATGCGCAATGTAAAGCCTATCCTCGCTGACGGCAGCTCGTCCAT
CCTCCATAAAACGCTTGAAACAAACCTGGAAAACAAGGCGATCTATATCGCCACGCCGTTGATCGTCTACATCAAGCAAG
GCAAGCAGATTATTCGCGACCATGACGCCATGGCCCATGTCGTCGAGGAAAATCATTTGATTTTCCTCTCCAAAGGCGTT
TACACGGTATCGGACTACGTCACTGGCAGCGATATCTTCGAGGCGGTGCTGCTGTTTTGCGACGACAAGCTGATCGCCAA
ATACCTGTCGCGCGCGGCCGGCACAGGCCATTCGCATACGGATGGCCTGCAGAACAACGCGCACGGCACTTATATCCTGC
ATGCCAATCAACAGATTCAGCGCTATATCGATTCGCTGAATTATGTCTACAAGGACGCGGAGGGCTCCGACGCGCTGCTG
GAATTGAAACTGCTCGAACTACTGCACCTGATCGCCATCCAGGACAAGTCCTTCCGTTTCCTGCGCGAGCTGTCCAGCGG
CGGCAGCCGCAAACGCCGCCCCATCACCAACTTCATGGAGCAGTACTATTCTCATAAGCTGAAGATTGAAGATTACGCCC
TGCTGACGGGACGCAGCGTGTCGACCTTTATCCGCGACTTCAGGCGCAGCTACAACACCACCCCGAACCGCTGGATCATC
GAGAAAAAAGTCGACATCGCGCATCAACTGTTGACAGCTAAAAATTATTCCGTCACCGACGCGGCAGCGGAGGTGGGATA
TGAAAATACCTCCCACTTCATCAAGGTCTACAAGCAACGCTACGGCGTCACGCCGAAGAAAGCCAAGGCCTTCGCAGCAG
AACTGTAG

Upstream 100 bases:

>100_bases
TTCCGTCCCAAACGTGCCGCACGCTTGGGCAGCAGCATGTCGCGCTGCTGGATACCGTCCTGTCCAAATGGGGCGGCCAA
TTTGTTGAGAAGCCATAGCA

Downstream 100 bases:

>100_bases
CGCGACAAGGCCCAAGCTCCCCGCTGCCGCGACAGCGGCATTGTGGGGCGTGATTGTAGGTGCCGACTAAATTTTGACCC
GAATGGATGATTCTAGCTGG

Product: transcriptional regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MTLEVVPAALYSMRNVKPILADGSSSILHKTLETNLENKAIYIATPLIVYIKQGKQIIRDHDAMAHVVEENHLIFLSKGV
YTVSDYVTGSDIFEAVLLFCDDKLIAKYLSRAAGTGHSHTDGLQNNAHGTYILHANQQIQRYIDSLNYVYKDAEGSDALL
ELKLLELLHLIAIQDKSFRFLRELSSGGSRKRRPITNFMEQYYSHKLKIEDYALLTGRSVSTFIRDFRRSYNTTPNRWII
EKKVDIAHQLLTAKNYSVTDAAAEVGYENTSHFIKVYKQRYGVTPKKAKAFAAEL

Sequences:

>Translated_295_residues
MTLEVVPAALYSMRNVKPILADGSSSILHKTLETNLENKAIYIATPLIVYIKQGKQIIRDHDAMAHVVEENHLIFLSKGV
YTVSDYVTGSDIFEAVLLFCDDKLIAKYLSRAAGTGHSHTDGLQNNAHGTYILHANQQIQRYIDSLNYVYKDAEGSDALL
ELKLLELLHLIAIQDKSFRFLRELSSGGSRKRRPITNFMEQYYSHKLKIEDYALLTGRSVSTFIRDFRRSYNTTPNRWII
EKKVDIAHQLLTAKNYSVTDAAAEVGYENTSHFIKVYKQRYGVTPKKAKAFAAEL
>Mature_294_residues
TLEVVPAALYSMRNVKPILADGSSSILHKTLETNLENKAIYIATPLIVYIKQGKQIIRDHDAMAHVVEENHLIFLSKGVY
TVSDYVTGSDIFEAVLLFCDDKLIAKYLSRAAGTGHSHTDGLQNNAHGTYILHANQQIQRYIDSLNYVYKDAEGSDALLE
LKLLELLHLIAIQDKSFRFLRELSSGGSRKRRPITNFMEQYYSHKLKIEDYALLTGRSVSTFIRDFRRSYNTTPNRWIIE
KKVDIAHQLLTAKNYSVTDAAAEVGYENTSHFIKVYKQRYGVTPKKAKAFAAEL

Specific function: Transcriptional activator of the thermally regulated virulent yopE gene. LcrF activity could be modulated by the interaction with an inducer molecule serving as a temperature messenger. The availability of the messenger would in turn be controlled by a te

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR003313
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR014710 [H]

Pfam domain/function: PF02311 AraC_binding; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 33509; Mature: 33378

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLEVVPAALYSMRNVKPILADGSSSILHKTLETNLENKAIYIATPLIVYIKQGKQIIRD
CCEEHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHH
HDAMAHVVEENHLIFLSKGVYTVSDYVTGSDIFEAVLLFCDDKLIAKYLSRAAGTGHSHT
HHHHHHHHCCCCEEEEECCCEEEHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCC
DGLQNNAHGTYILHANQQIQRYIDSLNYVYKDAEGSDALLELKLLELLHLIAIQDKSFRF
CCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
LRELSSGGSRKRRPITNFMEQYYSHKLKIEDYALLTGRSVSTFIRDFRRSYNTTPNRWII
HHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEE
EKKVDIAHQLLTAKNYSVTDAAAEVGYENTSHFIKVYKQRYGVTPKKAKAFAAEL
EHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure 
TLEVVPAALYSMRNVKPILADGSSSILHKTLETNLENKAIYIATPLIVYIKQGKQIIRD
CEEHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHH
HDAMAHVVEENHLIFLSKGVYTVSDYVTGSDIFEAVLLFCDDKLIAKYLSRAAGTGHSHT
HHHHHHHHCCCCEEEEECCCEEEHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCC
DGLQNNAHGTYILHANQQIQRYIDSLNYVYKDAEGSDALLELKLLELLHLIAIQDKSFRF
CCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
LRELSSGGSRKRRPITNFMEQYYSHKLKIEDYALLTGRSVSTFIRDFRRSYNTTPNRWII
HHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEE
EKKVDIAHQLLTAKNYSVTDAAAEVGYENTSHFIKVYKQRYGVTPKKAKAFAAEL
EHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1624422; 9746557; 9748454; 11586360 [H]