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The map label for this gene is hlyB [H]

Identifier: 34495850

GI number: 34495850

Start: 411276

End: 412694

Strand: Reverse

Name: hlyB [H]

Synonym: CV_0395

Alternate gene names: 34495850

Gene position: 412694-411276 (Counterclockwise)

Preceding gene: 34495851

Following gene: 34495847

Centisome position: 8.69

GC content: 68.36

Gene sequence:

>1419_bases
ATGAGTTCGCCGCGCAAACCCACCCCCTTCCTGCGCTCCCACCTGGCCTGGGTGCTGGGCGCCTTCAACCTGCTGGTGCT
GCTGTCGATCCTCGACGACTACCTGTTCCCGCCGCTGGCCCACCATGCGATCAGCGTGCTGCTGATGGCGTCCACGCTGG
CCCTGACCTGGTGGCTGTGGCAAAAGGGCGGCCGCATCTTCCTGCTGCTCAACACCTTGACCCGCCAGCTGGGCCTGGCC
TGCAGCGGCGAGCTGCATCACCGCAGCACCCAGACCCGCGCAATGGGCGAAGTCGGCCAGGTGGCCTGGCAGCTGAACGA
CTTCCTGGATCTGGTGGAGACCTACTTCAAGGAAATCAACACCTGCTTCCAGGAAATCAGCCGGGGCAACTTCAACCGCA
GGCCGCTGAGCCAGGGCCTGCCCGGCATCCTGTCCAGCTCGCTGGAGAGCGTCAACATCGCCATCCAGTCGATGGCGGAC
AACGACGGCTTCGTGCGCCGCAACCGGCTCTCCTCTCAGCTGGCCGCGCTGAACAACCCCCATCTGCGCCAGGACCTGGC
CGGCAGCCAGCGCGACCTCGCCGCCATCAGCCAGGCGATGGACCAGGTGGCCCGCATCACCCGCGACAACGCCGACGGCG
CCCGCGTCAGCCTGGACAGCGCCAACGAGCTGTCCGGCCACCTGGACACCATCGCCGGCAGCGTCAGCAGCATGGACACC
GCCAGCCAGATGCTGGCCCAGGAATGGCAAGGCATCGAGACTTCTCTGGCCGACATCTCCGCCATCGCCGACCAGACCAA
CCTGCTGGCCTTGAACGCCGCCATCGAGGCGGCGCGCGCCGGCGAAACCGGCCGCGGCTTCGCCGTGGTGGCCGACGAGG
TGAGAAAACTGGCCGAACGCAGCAAGAGCACCGCCCACCAAGTGCAGGGCGTGCTCGAGTCGCTATCCAGCCGCATCGGC
GACATGCAGGCCCAGGCCAGCGCCGCCGGCGGCGTGGCGGGCGAGGTGCAGTCGTCGGTGGACTCGTTCCGCCAGCGCTT
CTCCAGTCTGGCCGAGCAATCCGACCTGGTGCTGCAGCAGGTCAGCGCCGTGCAGCAGAAATCGCAGACCTCGCTGCAGA
AAGTGGGCCACGTGATCTACAAGCAGAGCGCCTACCACGCGATCGAGGAGGCGCAGGAAGCGCCCCCGGCTCTGGAGCTG
GAAGCCTGGCTGGAAGGCGATGGCGCGGCGCTGTTCGGCAGTGCCGCCCAGAACCTGCGGCCGCCGCTGCTGCAACTGCG
CCAGCAGGTGGACGCCGCGCTGCGCGCCGCCAGCCAGAACGGCCAGCTCGACGAAGCCGCCATCGTCGCCAGCATGCGCG
CGGTGGAACAGGCCAGCCAGAAGCTGCAATCGGGCCTGGAGGAGCTGGGCGGCCGCTGA

Upstream 100 bases:

>100_bases
GCGACTGGCTGCTGGCGCTGCTGGAAGAAAAAGGCTGCAGCTACCAGGACTTCATCCTGGAGCTGAACGCCGACGTGCTG
AAAGCCAAAGGAGTCGCCGG

Downstream 100 bases:

>100_bases
GCCTGCGCCGTCTATCCCAAAAGCCGGGCATGCCCGGCTTTTTTCATGCCCGGGCGACCAGCCTGGGCCAGCGTCAGTGC
CGGCTGGGCAGCCGATAGCG

Product: methyl-accepting chemotaxis protein IV

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSSPRKPTPFLRSHLAWVLGAFNLLVLLSILDDYLFPPLAHHAISVLLMASTLALTWWLWQKGGRIFLLLNTLTRQLGLA
CSGELHHRSTQTRAMGEVGQVAWQLNDFLDLVETYFKEINTCFQEISRGNFNRRPLSQGLPGILSSSLESVNIAIQSMAD
NDGFVRRNRLSSQLAALNNPHLRQDLAGSQRDLAAISQAMDQVARITRDNADGARVSLDSANELSGHLDTIAGSVSSMDT
ASQMLAQEWQGIETSLADISAIADQTNLLALNAAIEAARAGETGRGFAVVADEVRKLAERSKSTAHQVQGVLESLSSRIG
DMQAQASAAGGVAGEVQSSVDSFRQRFSSLAEQSDLVLQQVSAVQQKSQTSLQKVGHVIYKQSAYHAIEEAQEAPPALEL
EAWLEGDGAALFGSAAQNLRPPLLQLRQQVDAALRAASQNGQLDEAAIVASMRAVEQASQKLQSGLEELGGR

Sequences:

>Translated_472_residues
MSSPRKPTPFLRSHLAWVLGAFNLLVLLSILDDYLFPPLAHHAISVLLMASTLALTWWLWQKGGRIFLLLNTLTRQLGLA
CSGELHHRSTQTRAMGEVGQVAWQLNDFLDLVETYFKEINTCFQEISRGNFNRRPLSQGLPGILSSSLESVNIAIQSMAD
NDGFVRRNRLSSQLAALNNPHLRQDLAGSQRDLAAISQAMDQVARITRDNADGARVSLDSANELSGHLDTIAGSVSSMDT
ASQMLAQEWQGIETSLADISAIADQTNLLALNAAIEAARAGETGRGFAVVADEVRKLAERSKSTAHQVQGVLESLSSRIG
DMQAQASAAGGVAGEVQSSVDSFRQRFSSLAEQSDLVLQQVSAVQQKSQTSLQKVGHVIYKQSAYHAIEEAQEAPPALEL
EAWLEGDGAALFGSAAQNLRPPLLQLRQQVDAALRAASQNGQLDEAAIVASMRAVEQASQKLQSGLEELGGR
>Mature_471_residues
SSPRKPTPFLRSHLAWVLGAFNLLVLLSILDDYLFPPLAHHAISVLLMASTLALTWWLWQKGGRIFLLLNTLTRQLGLAC
SGELHHRSTQTRAMGEVGQVAWQLNDFLDLVETYFKEINTCFQEISRGNFNRRPLSQGLPGILSSSLESVNIAIQSMADN
DGFVRRNRLSSQLAALNNPHLRQDLAGSQRDLAAISQAMDQVARITRDNADGARVSLDSANELSGHLDTIAGSVSSMDTA
SQMLAQEWQGIETSLADISAIADQTNLLALNAAIEAARAGETGRGFAVVADEVRKLAERSKSTAHQVQGVLESLSSRIGD
MQAQASAAGGVAGEVQSSVDSFRQRFSSLAEQSDLVLQQVSAVQQKSQTSLQKVGHVIYKQSAYHAIEEAQEAPPALELE
AWLEGDGAALFGSAAQNLRPPLLQLRQQVDAALRAASQNGQLDEAAIVASMRAVEQASQKLQSGLEELGGR

Specific function: May form a pore through which the hemolysin can be exported [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1788194, Length=194, Percent_Identity=37.6288659793814, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787690, Length=342, Percent_Identity=30.4093567251462, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788195, Length=161, Percent_Identity=36.6459627329193, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1789453, Length=181, Percent_Identity=32.0441988950276, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI2367378, Length=164, Percent_Identity=34.7560975609756, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660
- InterPro:   IPR000727 [H]

Pfam domain/function: PF00672 HAMP; PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 50937; Mature: 50806

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPRKPTPFLRSHLAWVLGAFNLLVLLSILDDYLFPPLAHHAISVLLMASTLALTWWLW
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
QKGGRIFLLLNTLTRQLGLACSGELHHRSTQTRAMGEVGQVAWQLNDFLDLVETYFKEIN
HCCCEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TCFQEISRGNFNRRPLSQGLPGILSSSLESVNIAIQSMADNDGFVRRNRLSSQLAALNNP
HHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
HLRQDLAGSQRDLAAISQAMDQVARITRDNADGARVSLDSANELSGHLDTIAGSVSSMDT
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
ASQMLAQEWQGIETSLADISAIADQTNLLALNAAIEAARAGETGRGFAVVADEVRKLAER
HHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
SKSTAHQVQGVLESLSSRIGDMQAQASAAGGVAGEVQSSVDSFRQRFSSLAEQSDLVLQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSAVQQKSQTSLQKVGHVIYKQSAYHAIEEAQEAPPALELEAWLEGDGAALFGSAAQNLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCCCCHHHHHHHHCCC
PPLLQLRQQVDAALRAASQNGQLDEAAIVASMRAVEQASQKLQSGLEELGGR
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSPRKPTPFLRSHLAWVLGAFNLLVLLSILDDYLFPPLAHHAISVLLMASTLALTWWLW
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
QKGGRIFLLLNTLTRQLGLACSGELHHRSTQTRAMGEVGQVAWQLNDFLDLVETYFKEIN
HCCCEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TCFQEISRGNFNRRPLSQGLPGILSSSLESVNIAIQSMADNDGFVRRNRLSSQLAALNNP
HHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
HLRQDLAGSQRDLAAISQAMDQVARITRDNADGARVSLDSANELSGHLDTIAGSVSSMDT
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
ASQMLAQEWQGIETSLADISAIADQTNLLALNAAIEAARAGETGRGFAVVADEVRKLAER
HHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
SKSTAHQVQGVLESLSSRIGDMQAQASAAGGVAGEVQSSVDSFRQRFSSLAEQSDLVLQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSAVQQKSQTSLQKVGHVIYKQSAYHAIEEAQEAPPALELEAWLEGDGAALFGSAAQNLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCCCCHHHHHHHHCCC
PPLLQLRQQVDAALRAASQNGQLDEAAIVASMRAVEQASQKLQSGLEELGGR
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2162464; 10952301 [H]