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The map label for this gene is pgk [H]

Identifier: 33864370

GI number: 33864370

Start: 2219824

End: 2221029

Strand: Reverse

Name: pgk [H]

Synonym: PMT2106

Alternate gene names: 33864370

Gene position: 2221029-2219824 (Counterclockwise)

Preceding gene: 33864374

Following gene: 33864369

Centisome position: 92.13

GC content: 57.63

Gene sequence:

>1206_bases
ATGGCGAAGCGTTCACTGTCCAATCTCAGCGCAGCGGACCTACGCAGTAAGCGTGTGCTGGTTCGGGTTGATTTCAACGT
GCCTCTCAACGAGAACGGCGCCATTACCGATGACACCCGCATCCGCGCCGCCCTGCCCACCATCAATGACCTGATCGACA
AAGGTGCTCGGGTGATCCTCTCAGCCCACTTTGGCCGACCAAAGGGTCAAATCAACGAAGGAATGCGGCTCACACCAGTA
GCAAAACGCCTCAGCGAACTGCTGGGTAAAAACGTGACAAAAACCGAAAGCTGCGTTGGTAGCGACGCCGAAGCAAAGGT
TAATGCCATGGCCGATGGAGATGTCGTACTGCTCGAGAATGTGCGCTTTTTCTCAGAAGAAGAAAAAAACGACGCCAACT
TCGCCCAAAAGCTCGCAGCACTTGCTGACGCTTATGTCAATGACGCCTTCGGTGCTGCACACCGCGCCCACGCCTCTACA
GAAGGGGTCACCAAATTCCTCAACCCCAGTGTGGCAGGCCACCTGATGGAGAAGGAACTGCAATACCTGCAAGGTGCCAT
CGACGACCCGAAGCGACCCCTTGCAGCGATCGTGGGTGGGTCCAAGGTCAGCAGCAAGATCGGCGTGTTGGAAGCCTTGA
TCGACAAGTGCGACAAGGTGCTGATCGGCGGCGGCATGATCTTCACGTTCTACAAAGCGCGTGGCCTTTCAGTCGGCAAG
AGCCTGGTGGAAGAAGACAAGTTAGAGCTGGCCAAGGAGCTAGAGGCCAAGGCGAAGGCCAAGGGGGTTCAACTGCTTCT
GCCCACTGACGTTGTGCTAGCCGATGACTTCGCCCCCGCCGCCAATAGCCAGACCGTATCCATCGACGCCATTCCCGATG
GTTGGATGGGACTGGACATTGGCCCCGACTCAATCAATCTCTTCCAAGACGCACTGGCTGACTGCCAAACCGTGATCTGG
AACGGCCCCATGGGCGTGTTCGAGTTCGATAAGTTTGCCGCCGGCACCAACGCCATCGCCACAACCTTGGCGGACCTCAG
TGGCAAGGGTTGCTGCACAATTATTGGTGGTGGTGACTCCGTAGCTGCTGTTGAGAAGGCCGGCCTCGCCGAGAAGATGT
CCCACATCTCCACCGGTGGTGGCGCCAGCCTTGAGCTGCTGGAAGGCAAGGTGTTGCCAGGTGTCGCCGCTCTCGACGAC
GCCTAA

Upstream 100 bases:

>100_bases
TAGCGCCAACCAGATCAGTGCGCCGGCCCATCAAGCCAACCAGCCAAACAAAACACGGGTTAATGTCAGCGGGTTTTTCT
TACCGTACGGCACAACCCCC

Downstream 100 bases:

>100_bases
GCAAAGACCTCGCTTGTCTTATCGGGCCATTGCTTGCATCACTGCAATGGCCCCGGCATTCATTAGCTCTGATAAGAGCT
CTCGAGCAAACTTCCGGCGG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MAKRSLSNLSAADLRSKRVLVRVDFNVPLNENGAITDDTRIRAALPTINDLIDKGARVILSAHFGRPKGQINEGMRLTPV
AKRLSELLGKNVTKTESCVGSDAEAKVNAMADGDVVLLENVRFFSEEEKNDANFAQKLAALADAYVNDAFGAAHRAHAST
EGVTKFLNPSVAGHLMEKELQYLQGAIDDPKRPLAAIVGGSKVSSKIGVLEALIDKCDKVLIGGGMIFTFYKARGLSVGK
SLVEEDKLELAKELEAKAKAKGVQLLLPTDVVLADDFAPAANSQTVSIDAIPDGWMGLDIGPDSINLFQDALADCQTVIW
NGPMGVFEFDKFAAGTNAIATTLADLSGKGCCTIIGGGDSVAAVEKAGLAEKMSHISTGGGASLELLEGKVLPGVAALDD
A

Sequences:

>Translated_401_residues
MAKRSLSNLSAADLRSKRVLVRVDFNVPLNENGAITDDTRIRAALPTINDLIDKGARVILSAHFGRPKGQINEGMRLTPV
AKRLSELLGKNVTKTESCVGSDAEAKVNAMADGDVVLLENVRFFSEEEKNDANFAQKLAALADAYVNDAFGAAHRAHAST
EGVTKFLNPSVAGHLMEKELQYLQGAIDDPKRPLAAIVGGSKVSSKIGVLEALIDKCDKVLIGGGMIFTFYKARGLSVGK
SLVEEDKLELAKELEAKAKAKGVQLLLPTDVVLADDFAPAANSQTVSIDAIPDGWMGLDIGPDSINLFQDALADCQTVIW
NGPMGVFEFDKFAAGTNAIATTLADLSGKGCCTIIGGGDSVAAVEKAGLAEKMSHISTGGGASLELLEGKVLPGVAALDD
A
>Mature_400_residues
AKRSLSNLSAADLRSKRVLVRVDFNVPLNENGAITDDTRIRAALPTINDLIDKGARVILSAHFGRPKGQINEGMRLTPVA
KRLSELLGKNVTKTESCVGSDAEAKVNAMADGDVVLLENVRFFSEEEKNDANFAQKLAALADAYVNDAFGAAHRAHASTE
GVTKFLNPSVAGHLMEKELQYLQGAIDDPKRPLAAIVGGSKVSSKIGVLEALIDKCDKVLIGGGMIFTFYKARGLSVGKS
LVEEDKLELAKELEAKAKAKGVQLLLPTDVVLADDFAPAANSQTVSIDAIPDGWMGLDIGPDSINLFQDALADCQTVIWN
GPMGVFEFDKFAAGTNAIATTLADLSGKGCCTIIGGGDSVAAVEKAGLAEKMSHISTGGGASLELLEGKVLPGVAALDDA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=418, Percent_Identity=45.6937799043062, Blast_Score=345, Evalue=6e-95,
Organism=Homo sapiens, GI31543397, Length=411, Percent_Identity=43.7956204379562, Blast_Score=337, Evalue=2e-92,
Organism=Escherichia coli, GI1789294, Length=398, Percent_Identity=42.964824120603, Blast_Score=299, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI17508823, Length=407, Percent_Identity=48.6486486486487, Blast_Score=366, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6319857, Length=405, Percent_Identity=49.3827160493827, Blast_Score=372, Evalue=1e-104,
Organism=Drosophila melanogaster, GI17136394, Length=417, Percent_Identity=47.242206235012, Blast_Score=334, Evalue=7e-92,
Organism=Drosophila melanogaster, GI24581203, Length=418, Percent_Identity=43.3014354066986, Blast_Score=316, Evalue=2e-86,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 42104; Mature: 41972

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRSLSNLSAADLRSKRVLVRVDFNVPLNENGAITDDTRIRAALPTINDLIDKGARVIL
CCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCEECCCHHHHHHHCCCEEEE
SAHFGRPKGQINEGMRLTPVAKRLSELLGKNVTKTESCVGSDAEAKVNAMADGDVVLLEN
EECCCCCCCHHCCCCEECHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEECCCCEEEEEC
VRFFSEEEKNDANFAQKLAALADAYVNDAFGAAHRAHASTEGVTKFLNPSVAGHLMEKEL
CHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHH
QYLQGAIDDPKRPLAAIVGGSKVSSKIGVLEALIDKCDKVLIGGGMIFTFYKARGLSVGK
HHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCEEECCCEEEEHHHHCCCHHHH
SLVEEDKLELAKELEAKAKAKGVQLLLPTDVVLADDFAPAANSQTVSIDAIPDGWMGLDI
HHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCCCCCCCCCEEEEECCCCCCEEEEE
GPDSINLFQDALADCQTVIWNGPMGVFEFDKFAAGTNAIATTLADLSGKGCCTIIGGGDS
CCCHHHHHHHHHHHHHHEEECCCCCCEEHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCC
VAAVEKAGLAEKMSHISTGGGASLELLEGKVLPGVAALDDA
CHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHCCCC
>Mature Secondary Structure 
AKRSLSNLSAADLRSKRVLVRVDFNVPLNENGAITDDTRIRAALPTINDLIDKGARVIL
CCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCEECCCHHHHHHHCCCEEEE
SAHFGRPKGQINEGMRLTPVAKRLSELLGKNVTKTESCVGSDAEAKVNAMADGDVVLLEN
EECCCCCCCHHCCCCEECHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEECCCCEEEEEC
VRFFSEEEKNDANFAQKLAALADAYVNDAFGAAHRAHASTEGVTKFLNPSVAGHLMEKEL
CHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHH
QYLQGAIDDPKRPLAAIVGGSKVSSKIGVLEALIDKCDKVLIGGGMIFTFYKARGLSVGK
HHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCEEECCCEEEEHHHHCCCHHHH
SLVEEDKLELAKELEAKAKAKGVQLLLPTDVVLADDFAPAANSQTVSIDAIPDGWMGLDI
HHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCCCCCCCCCEEEEECCCCCCEEEEE
GPDSINLFQDALADCQTVIWNGPMGVFEFDKFAAGTNAIATTLADLSGKGCCTIIGGGDS
CCCHHHHHHHHHHHHHHEEECCCCCCEEHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCC
VAAVEKAGLAEKMSHISTGGGASLELLEGKVLPGVAALDDA
CHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA