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The map label for this gene is Hgd [H]

Identifier: 33864369

GI number: 33864369

Start: 2218569

End: 2219411

Strand: Reverse

Name: Hgd [H]

Synonym: PMT2105

Alternate gene names: 33864369

Gene position: 2219411-2218569 (Counterclockwise)

Preceding gene: 33864370

Following gene: 33864368

Centisome position: 92.06

GC content: 55.99

Gene sequence:

>843_bases
ATGGCTGCCAATCTGCTGGCTGCGGGCTATGAGCTGAAGGTTCACAGCCGCAGTCGTGCGGCTGAACAGAATTTGGCTTT
GCAAGGTAGCCGTTCCTGTAGCTCACCAGCAGAAGCTGCAGCAGACAGCCAGGCTCTACTGATCTGCGTTAGCGATGATG
AAGCAGTAGAAGAGGTTCTTTTTGGTTCTCAGGGAGCAGCTTCTATGCTCAGCTCCGGCGCCGTTGTGGTGGATTTTTCG
ACGATCACCCCAGCAACGTCGATAAGCATGGCTGAGCGACTTGCTGATCAAGGGGTGACCTATCTGGATGCACCGGTGAC
CGGAGGCACTGAAGGGGCCAGAGCCGGAACTCTTACCGTTCTAGTGGGTGGCAACAAACAAGCTCTGGCAAGGGTGCAGC
CACTGCTGGAAGTGATCGGTGAGCGCATTCATCACTTCGGTTCGGTGGGACGGGGCCAGCAGGTGAAGGCGCTCAACCAA
GTGCTGGTGGCCGGTAGCTATGCCGCATTAGCAGAAGCAATCGCGCTAGGGCAGCAGTTGGGTCTCCCTATGCAGGAGGT
GATCACAGCCTTACAGCATGGTGCCGCAGGCTCATGGGCCCTCAAGCACCGATCAACAGCGATGCTGGAAGATCACTATC
CGCTCGGCTTCAAGCTGGCTTTACATCACAAGGATCTCGGCATTGCCCTTGAAACGGCAGAACGCGCGGGACTACAACTG
CCAATCACAAGCCAAGTGAAATCCATGGAGGCAAACCTGATTGAACACGGCCATAGCGAGGAAGATGTCTCAGTGCTGAG
GCGGTGGTTTGATCAGCAACAGGCAGAATCTCAGCCATTCTGA

Upstream 100 bases:

>100_bases
AGTAAAAATCGAAGCAGCTCTACTGACAAACATGAGCAAAGCGCAGCTAAGCCCAAGGTCGAGCCTTGCCTTTGTTGGCC
TCGGCGCATTGGGTTTACCA

Downstream 100 bases:

>100_bases
TCGAGCCTTCGATAGGCTGAACAAGTTGTTCATTACTGCTGCAATTGAAGGTTAGTTGTTTCATTAAGATCAACGCAAGT
CTATTGAATATAACGACTTT

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MAANLLAAGYELKVHSRSRAAEQNLALQGSRSCSSPAEAAADSQALLICVSDDEAVEEVLFGSQGAASMLSSGAVVVDFS
TITPATSISMAERLADQGVTYLDAPVTGGTEGARAGTLTVLVGGNKQALARVQPLLEVIGERIHHFGSVGRGQQVKALNQ
VLVAGSYAALAEAIALGQQLGLPMQEVITALQHGAAGSWALKHRSTAMLEDHYPLGFKLALHHKDLGIALETAERAGLQL
PITSQVKSMEANLIEHGHSEEDVSVLRRWFDQQQAESQPF

Sequences:

>Translated_280_residues
MAANLLAAGYELKVHSRSRAAEQNLALQGSRSCSSPAEAAADSQALLICVSDDEAVEEVLFGSQGAASMLSSGAVVVDFS
TITPATSISMAERLADQGVTYLDAPVTGGTEGARAGTLTVLVGGNKQALARVQPLLEVIGERIHHFGSVGRGQQVKALNQ
VLVAGSYAALAEAIALGQQLGLPMQEVITALQHGAAGSWALKHRSTAMLEDHYPLGFKLALHHKDLGIALETAERAGLQL
PITSQVKSMEANLIEHGHSEEDVSVLRRWFDQQQAESQPF
>Mature_279_residues
AANLLAAGYELKVHSRSRAAEQNLALQGSRSCSSPAEAAADSQALLICVSDDEAVEEVLFGSQGAASMLSSGAVVVDFST
ITPATSISMAERLADQGVTYLDAPVTGGTEGARAGTLTVLVGGNKQALARVQPLLEVIGERIHHFGSVGRGQQVKALNQV
LVAGSYAALAEAIALGQQLGLPMQEVITALQHGAAGSWALKHRSTAMLEDHYPLGFKLALHHKDLGIALETAERAGLQLP
ITSQVKSMEANLIEHGHSEEDVSVLRRWFDQQQAESQPF

Specific function: Catalyzes the conversion of 2-formylglutarate to (S)-2- hydroxymethylglutarate. Has very low activity with (S)-3- hydroxyisobutyrate [H]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=281, Percent_Identity=28.8256227758007, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI40556376, Length=271, Percent_Identity=23.9852398523985, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1786719, Length=266, Percent_Identity=37.218045112782, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI145693186, Length=272, Percent_Identity=33.4558823529412, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790315, Length=272, Percent_Identity=30.8823529411765, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1789092, Length=228, Percent_Identity=32.4561403508772, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17557316, Length=286, Percent_Identity=26.9230769230769, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24655230, Length=281, Percent_Identity=28.1138790035587, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19922568, Length=281, Percent_Identity=28.1138790035587, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI28574115, Length=267, Percent_Identity=23.5955056179775, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.291 [H]

Molecular weight: Translated: 29482; Mature: 29350

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAANLLAAGYELKVHSRSRAAEQNLALQGSRSCSSPAEAAADSQALLICVSDDEAVEEVL
CCCCCEECCEEEEECCHHHHHHHHHEECCCCCCCCHHHHCCCCCEEEEEECCHHHHHHHH
FGSQGAASMLSSGAVVVDFSTITPATSISMAERLADQGVTYLDAPVTGGTEGARAGTLTV
CCCCCHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCEEEE
LVGGNKQALARVQPLLEVIGERIHHFGSVGRGQQVKALNQVLVAGSYAALAEAIALGQQL
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
GLPMQEVITALQHGAAGSWALKHRSTAMLEDHYPLGFKLALHHKDLGIALETAERAGLQL
CCCHHHHHHHHHCCCCCCEEHHHCCHHHCCCCCCCCEEEEEEECCCCEEEECHHHCCCCC
PITSQVKSMEANLIEHGHSEEDVSVLRRWFDQQQAESQPF
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AANLLAAGYELKVHSRSRAAEQNLALQGSRSCSSPAEAAADSQALLICVSDDEAVEEVL
CCCCEECCEEEEECCHHHHHHHHHEECCCCCCCCHHHHCCCCCEEEEEECCHHHHHHHH
FGSQGAASMLSSGAVVVDFSTITPATSISMAERLADQGVTYLDAPVTGGTEGARAGTLTV
CCCCCHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCEEEE
LVGGNKQALARVQPLLEVIGERIHHFGSVGRGQQVKALNQVLVAGSYAALAEAIALGQQL
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
GLPMQEVITALQHGAAGSWALKHRSTAMLEDHYPLGFKLALHHKDLGIALETAERAGLQL
CCCHHHHHHHHHCCCCCCEEHHHCCHHHCCCCCCCCEEEEEEECCCCEEEECHHHCCCCC
PITSQVKSMEANLIEHGHSEEDVSVLRRWFDQQQAESQPF
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA