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The map label for this gene is rnhA [H]

Identifier: 33864357

GI number: 33864357

Start: 2205905

End: 2206678

Strand: Direct

Name: rnhA [H]

Synonym: PMT2093

Alternate gene names: 33864357

Gene position: 2205905-2206678 (Clockwise)

Preceding gene: 33864355

Following gene: 33864358

Centisome position: 91.5

GC content: 56.07

Gene sequence:

>774_bases
ATGGCTGATGGTCGTGGACGGGTTGTGGCTGCTGCCACTGATGGGGCTTGTAGTGGAAATCCTGGCCCTGGGGGCTGGGG
GGCACTGCTGCGCTTTGAGGATGGGAGCGTGGAGGAGTTCGGAGGCTATGCGCCGGCCACGACCAACAACCGCATGGAAC
TTCAGGCGGCCCTTCATGTGCTGGAGCAGCTCAAAGAGCTTCCTTGTCACCCCGATTTAAAGATCCGTACCGACAGCAAA
TATTTAATTGATGGTTTGAGCAAATGGATGGCGGGATGGAAACGTAAGGGTTGGCGTACGGCGGCTGGCAAGCCTGTGCT
GAATCAAGATCTCTGGCGAGCTCTAGACCGTGCTCGCTTGGATCATGTGCCCCTGGCTTATGTGAAAGGTCATAGCGGTG
ACCCAGACAATGAAAGGGTTGACCAAATCGCAGTGAGCTTTTCCAAAGGAGGTGGTACTCCAGCGTTGATGTCTGGACAG
GGGTCAGCCTCTATTGCACTCGGTCTTGTTGAGCCGATCAAAGAAGCTGGAGATGGCGAAGATTTTGCCCCTTCTGATTT
ACAGCGGCTGCTAACAAGACTGGAGCTTGCTGATCGTTTCGCTGTGCGTGGCTATGGCCTCAGCCTGATTGAGGTGGCCC
AATTGGTGGAGCAACCAATCGAGCAGCTTGCGAATAAGACTGCCCCCTGGCGTTGGCGCGATTGGCTATTGGAGCCTGTG
GGGGAAAACCGCTGGCGATTGCACCGCTGCGCGGCAGGATCGGATCAGATCTGA

Upstream 100 bases:

>100_bases
TCTCAATGCCATGAGCAGCTCGGGGTAGGTTCGCGACATAGCGTCTAATGACTAAGAACCAAGCGAACCGTGTTGTACCA
AACAACAACAGACTTCTCAA

Downstream 100 bases:

>100_bases
GGAGGCCCAGGATGGCTGAGCCATCACCTGCAAAGGTGTACTCCACCGGTGGGTTGTATCGCCGTTGGCTGGGAGCAATA
CTCGCGAATGATCAGGGCTT

Product: ribonuclease HI

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MADGRGRVVAAATDGACSGNPGPGGWGALLRFEDGSVEEFGGYAPATTNNRMELQAALHVLEQLKELPCHPDLKIRTDSK
YLIDGLSKWMAGWKRKGWRTAAGKPVLNQDLWRALDRARLDHVPLAYVKGHSGDPDNERVDQIAVSFSKGGGTPALMSGQ
GSASIALGLVEPIKEAGDGEDFAPSDLQRLLTRLELADRFAVRGYGLSLIEVAQLVEQPIEQLANKTAPWRWRDWLLEPV
GENRWRLHRCAAGSDQI

Sequences:

>Translated_257_residues
MADGRGRVVAAATDGACSGNPGPGGWGALLRFEDGSVEEFGGYAPATTNNRMELQAALHVLEQLKELPCHPDLKIRTDSK
YLIDGLSKWMAGWKRKGWRTAAGKPVLNQDLWRALDRARLDHVPLAYVKGHSGDPDNERVDQIAVSFSKGGGTPALMSGQ
GSASIALGLVEPIKEAGDGEDFAPSDLQRLLTRLELADRFAVRGYGLSLIEVAQLVEQPIEQLANKTAPWRWRDWLLEPV
GENRWRLHRCAAGSDQI
>Mature_256_residues
ADGRGRVVAAATDGACSGNPGPGGWGALLRFEDGSVEEFGGYAPATTNNRMELQAALHVLEQLKELPCHPDLKIRTDSKY
LIDGLSKWMAGWKRKGWRTAAGKPVLNQDLWRALDRARLDHVPLAYVKGHSGDPDNERVDQIAVSFSKGGGTPALMSGQG
SASIALGLVEPIKEAGDGEDFAPSDLQRLLTRLELADRFAVRGYGLSLIEVAQLVEQPIEQLANKTAPWRWRDWLLEPVG
ENRWRLHRCAAGSDQI

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=149, Percent_Identity=36.9127516778523, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1786408, Length=139, Percent_Identity=48.9208633093525, Blast_Score=133, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI115532542, Length=136, Percent_Identity=41.1764705882353, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17505482, Length=130, Percent_Identity=37.6923076923077, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45552509, Length=151, Percent_Identity=33.112582781457, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI17137476, Length=151, Percent_Identity=33.112582781457, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 28058; Mature: 27927

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADGRGRVVAAATDGACSGNPGPGGWGALLRFEDGSVEEFGGYAPATTNNRMELQAALHV
CCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHH
LEQLKELPCHPDLKIRTDSKYLIDGLSKWMAGWKRKGWRTAAGKPVLNQDLWRALDRARL
HHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
DHVPLAYVKGHSGDPDNERVDQIAVSFSKGGGTPALMSGQGSASIALGLVEPIKEAGDGE
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCEEHHHHHHHHHCCCCC
DFAPSDLQRLLTRLELADRFAVRGYGLSLIEVAQLVEQPIEQLANKTAPWRWRDWLLEPV
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCC
GENRWRLHRCAAGSDQI
CCCCCEEHEECCCCCCC
>Mature Secondary Structure 
ADGRGRVVAAATDGACSGNPGPGGWGALLRFEDGSVEEFGGYAPATTNNRMELQAALHV
CCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHH
LEQLKELPCHPDLKIRTDSKYLIDGLSKWMAGWKRKGWRTAAGKPVLNQDLWRALDRARL
HHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
DHVPLAYVKGHSGDPDNERVDQIAVSFSKGGGTPALMSGQGSASIALGLVEPIKEAGDGE
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCEEHHHHHHHHHCCCCC
DFAPSDLQRLLTRLELADRFAVRGYGLSLIEVAQLVEQPIEQLANKTAPWRWRDWLLEPV
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCC
GENRWRLHRCAAGSDQI
CCCCCEEHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917486 [H]