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The map label for this gene is cca [H]

Identifier: 33864266

GI number: 33864266

Start: 2114726

End: 2115964

Strand: Direct

Name: cca [H]

Synonym: PMT2001

Alternate gene names: 33864266

Gene position: 2114726-2115964 (Clockwise)

Preceding gene: 33864263

Following gene: 33864267

Centisome position: 87.72

GC content: 56.26

Gene sequence:

>1239_bases
ATGCCCTCCCTTCGCTCTGCTGCACCCATTCTGGAGGGTGATCCCACCATCCGGGTCCAGGCCCTTTGGGAACAATTACA
TCCTGATCACTGGCCACTATCGATTCAAGACCTTCCTGCTGGAACGGTCTTAGTAGGAGGTGCCGTTCGTGATGGCCTGC
TTGGCCGACTTCAGCAGCAACCAGATCTCGACTTCATTGTGCCAAACAAGGCGCTAGAGATCACCCGCAGCCTGGCCAAG
ACACTGGGAGGCACATGTGTGGTGCTGGACGCCGAAAGGGACATGGCGCGATTGGTCATCAAAGGCTGGACCATCGACAT
CGCCGCCCAAGAGGGATCCAACCTTGACGAGGACCTTTGGCGGCGGGACTTCCGTCTCAACGCCATTGCCCTCACCCTCG
AGGCCACCCCTCAGCTCGTGGACCCAACGGGAGGGCTAAGTGATCTGGAGCAAAAAAAACTTGTTGCGGTACGTGAGCAA
AACCTGCTCGACGATCCCCTGAGGTTGCTGCGCGGTCTGCGACTGATGGCCGAACTGCAGCTCAGCCTGGGAAAGCAAAC
CTTGGCATGGATCAATTGCCACCACAGACTTCTGCCCCAAGCAGCTCCTGAACGAATTCAGGCAGAACTACAACGGATCG
TGGCTGCGCCTTGGGCCGATGACGTGCTGCCGCTGCTGCTGCGCACTGGCCTACTGGATTGTTGGCAAAACACCATTGAA
AGAATCAGCCGACCAGCACCATGTTTAAAGGTTGCCAAATCCCTCAAACCTGAAGAGCAAGCCGTTGCCCTTCCCTTGGC
TCGTCTCACTCATCTGCTCAGCAATGACGGGCTGAGCGCATTGCGCTTCAGCCGACGACAATGTCAACGATGCCAACTCC
TTCGTCACTGGGAAGAACGCAACGACGGTATGGCGTTTGCAAGCCTCAGCGAATTGGATCGGCTGCAATTGCACCTCGAT
CTAGAAACAGACCTTCCAGCCCTCATCCTGCAGCTCCCGCACATCAGCCAGGTGCAGTGGCTAGAGCGTTGGCGGAACGC
GGATGATCCTCTGTTCCACCCGTCACCACCTGTGGACGGCTATGCACTGCAAAAGACCCTCAAACTCCCCAAAGGACAAG
CTCTAGGTGAGCTGCTTCGCCATCTCTGCCAAGAAAAAGCCTTTGGTCGGGTACAGAATCGAAAGCAAGCTTTTAAGGCG
GCAACTTACTGGTGGGAACAAAAACAAACCTTGCTGTGA

Upstream 100 bases:

>100_bases
CAGATCCGGTGCCGCATTGAGATGGGCAACCCCGTGCTGCCGCTCCACCGCGTGATAGGCCTGGCAACGATCAACCCAGC
GGCAGTCGACACAAATACAC

Downstream 100 bases:

>100_bases
TTAACTGCACTGGCAATAAGGACATCGATCGGCCTGATCCACATCCTTTTCTCATTCCCCTCTCTTTTTCATGAGCGTTC
GCCTTTACATCGGCAACCTG

Product: poly A polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MPSLRSAAPILEGDPTIRVQALWEQLHPDHWPLSIQDLPAGTVLVGGAVRDGLLGRLQQQPDLDFIVPNKALEITRSLAK
TLGGTCVVLDAERDMARLVIKGWTIDIAAQEGSNLDEDLWRRDFRLNAIALTLEATPQLVDPTGGLSDLEQKKLVAVREQ
NLLDDPLRLLRGLRLMAELQLSLGKQTLAWINCHHRLLPQAAPERIQAELQRIVAAPWADDVLPLLLRTGLLDCWQNTIE
RISRPAPCLKVAKSLKPEEQAVALPLARLTHLLSNDGLSALRFSRRQCQRCQLLRHWEERNDGMAFASLSELDRLQLHLD
LETDLPALILQLPHISQVQWLERWRNADDPLFHPSPPVDGYALQKTLKLPKGQALGELLRHLCQEKAFGRVQNRKQAFKA
ATYWWEQKQTLL

Sequences:

>Translated_412_residues
MPSLRSAAPILEGDPTIRVQALWEQLHPDHWPLSIQDLPAGTVLVGGAVRDGLLGRLQQQPDLDFIVPNKALEITRSLAK
TLGGTCVVLDAERDMARLVIKGWTIDIAAQEGSNLDEDLWRRDFRLNAIALTLEATPQLVDPTGGLSDLEQKKLVAVREQ
NLLDDPLRLLRGLRLMAELQLSLGKQTLAWINCHHRLLPQAAPERIQAELQRIVAAPWADDVLPLLLRTGLLDCWQNTIE
RISRPAPCLKVAKSLKPEEQAVALPLARLTHLLSNDGLSALRFSRRQCQRCQLLRHWEERNDGMAFASLSELDRLQLHLD
LETDLPALILQLPHISQVQWLERWRNADDPLFHPSPPVDGYALQKTLKLPKGQALGELLRHLCQEKAFGRVQNRKQAFKA
ATYWWEQKQTLL
>Mature_411_residues
PSLRSAAPILEGDPTIRVQALWEQLHPDHWPLSIQDLPAGTVLVGGAVRDGLLGRLQQQPDLDFIVPNKALEITRSLAKT
LGGTCVVLDAERDMARLVIKGWTIDIAAQEGSNLDEDLWRRDFRLNAIALTLEATPQLVDPTGGLSDLEQKKLVAVREQN
LLDDPLRLLRGLRLMAELQLSLGKQTLAWINCHHRLLPQAAPERIQAELQRIVAAPWADDVLPLLLRTGLLDCWQNTIER
ISRPAPCLKVAKSLKPEEQAVALPLARLTHLLSNDGLSALRFSRRQCQRCQLLRHWEERNDGMAFASLSELDRLQLHLDL
ETDLPALILQLPHISQVQWLERWRNADDPLFHPSPPVDGYALQKTLKLPKGQALGELLRHLCQEKAFGRVQNRKQAFKAA
TYWWEQKQTLL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46539; Mature: 46407

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLRSAAPILEGDPTIRVQALWEQLHPDHWPLSIQDLPAGTVLVGGAVRDGLLGRLQQQ
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCC
PDLDFIVPNKALEITRSLAKTLGGTCVVLDAERDMARLVIKGWTIDIAAQEGSNLDEDLW
CCCCEECCCHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHCCCEEEEEECCCCCCCHHHH
RRDFRLNAIALTLEATPQLVDPTGGLSDLEQKKLVAVREQNLLDDPLRLLRGLRLMAELQ
HHCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LSLGKQTLAWINCHHRLLPQAAPERIQAELQRIVAAPWADDVLPLLLRTGLLDCWQNTIE
HHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RISRPAPCLKVAKSLKPEEQAVALPLARLTHLLSNDGLSALRFSRRQCQRCQLLRHWEER
HHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
NDGMAFASLSELDRLQLHLDLETDLPALILQLPHISQVQWLERWRNADDPLFHPSPPVDG
CCCCCHHHHHHHHHEEEEEECCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
YALQKTLKLPKGQALGELLRHLCQEKAFGRVQNRKQAFKAATYWWEQKQTLL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSLRSAAPILEGDPTIRVQALWEQLHPDHWPLSIQDLPAGTVLVGGAVRDGLLGRLQQQ
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCC
PDLDFIVPNKALEITRSLAKTLGGTCVVLDAERDMARLVIKGWTIDIAAQEGSNLDEDLW
CCCCEECCCHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHCCCEEEEEECCCCCCCHHHH
RRDFRLNAIALTLEATPQLVDPTGGLSDLEQKKLVAVREQNLLDDPLRLLRGLRLMAELQ
HHCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LSLGKQTLAWINCHHRLLPQAAPERIQAELQRIVAAPWADDVLPLLLRTGLLDCWQNTIE
HHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RISRPAPCLKVAKSLKPEEQAVALPLARLTHLLSNDGLSALRFSRRQCQRCQLLRHWEER
HHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
NDGMAFASLSELDRLQLHLDLETDLPALILQLPHISQVQWLERWRNADDPLFHPSPPVDG
CCCCCHHHHHHHHHEEEEEECCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
YALQKTLKLPKGQALGELLRHLCQEKAFGRVQNRKQAFKAATYWWEQKQTLL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12397186 [H]