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The map label for this gene is radA [H]

Identifier: 33864258

GI number: 33864258

Start: 2106948

End: 2108342

Strand: Reverse

Name: radA [H]

Synonym: PMT1993

Alternate gene names: 33864258

Gene position: 2108342-2106948 (Counterclockwise)

Preceding gene: 33864264

Following gene: 33864256

Centisome position: 87.45

GC content: 56.99

Gene sequence:

>1395_bases
GTGCGCACTGCTTCCGTCTACGTCTGTCAGAGCTGTGGCGCTAAGACCAGCCAGTTTTTTGGTCGCTGCGCCAGTTGCGG
GACTTGGAATTCTTTGGTCGAACAGGTCGCTCATTCAACCGATGGCCGCCGCCGCAGTAACAGTTTTGATCCGGCCGTGG
AACCAGTGGCGCGGCGCTCGATGGCGATGGCTTCTCTCGGGGATCAACCCGTTCGCCGTCTTGCCAGTGGCTACGAAGAG
TTGGATCGAGTCCTGGGCGGCGGGCTGGTGCCTGGATCAATGGTGTTAGTTGGAGGTGATCCTGGGATTGGCAAGAGCAC
GTTGCTGTTGCAGAGCGCCACGGCTATGGCACTGCAACACTCTGTGCTTTACGTGAGTGCTGAGGAGTCTGCCCAGCAGG
TGAAGTTGCGTTGGCTGCGCCTTGAGGGGGTTACGTCGGACCTGCAGCTTTTGGCGGAAACGGACTTGGAATTGGTGCTT
GAGGAATTGGAAGCACTGCGGCCTGCGGTGGCAATTATCGACAGCATTCAGGCTTTGCATGATGGGTCGCTTTCCAGTGC
GCCTGGCTCTGTCGCTCAGGTGCGCGAGTGTGCAGCAGCTTTACAGCGTTTGGCTAAGCGTCAGGAAACAGCCTTGGTTT
TGGTGGGCCATGTCACCAAGGAGGGGATGCTGGCGGGCCCCAAGGTGCTTGAGCATTTGGTCGATGCAGTACTCACTTTT
GAGGGCGATCGATTTGCCAGTCATCGCCTTCTGAGGGCTGTAAAAAATCGCTTTGGAGCCACCCATGAGCTTGGGGTGTT
CGAGATGCAGGGGAAGGGCTTAGCTGAAGTCGGGAATCCAAGCGAGCTCTTTCTCAAGGGTGAGTCTGCTTCTGGGGTGG
CCACCATCGTGGCCTTTGAAGGCACCCGTTCATTGGTGGTGGATCTGCAGGCTTTGGTGGGTGTAACCAGCTATGCCAGT
CCTCGCCGTACCGCTACAGGATTAGGCACCAACCGCTTGCACCAAATTCTGGCCGTATTGGAGAAGCACATGGGCTTGCC
ATTGTCACGCTATGACTGTTATCTGGCTGTTGCTGGCGGATTAGAAGTCGAAGAGCCTGCTGCTGATTTGGGTGTCGCCG
CTGCCGTTGTTTCCAGTTATCGAGATCTCATGCTGCCAAAAGGCACAGTTTTGCTTGGGGAGTTGGGCTTAGGGGGGCAA
TTGAGGCCAGTGGGGCAGCTTGGGCAGCGTCTTAAGGAAGCAGTTCGCTTAGGTTTTCATCGTGCAGTAGTGCCGCAGGG
CAGTGGCCTAGGACCCATCGGCAAGGAGCTGGAGTTGGAACTACTTGAGGCTGCGAGTGTGACTGAGGCTTTGGTCATGG
CCCTCGGTGTGAATCCAGCTGATGATGGCTGCTGA

Upstream 100 bases:

>100_bases
TTCTTTGGCGGCTGCAATCAATGAATCTTAGATAAGAGCTGTTAAGGCGTACCGGCCTTTTGGTTCATTTAAAGCCAACT
TTCTTTACCTATCTTAGTAA

Downstream 100 bases:

>100_bases
AGGATGTTTTCGCTTTAGAAATAGACGTCTATGTTGCTTCGGCCTGTTGTTTTCAGCCAGTTCTCAATATCTAGGTAGCC
GCCGGGATAGAGCCTTACCG

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MRTASVYVCQSCGAKTSQFFGRCASCGTWNSLVEQVAHSTDGRRRSNSFDPAVEPVARRSMAMASLGDQPVRRLASGYEE
LDRVLGGGLVPGSMVLVGGDPGIGKSTLLLQSATAMALQHSVLYVSAEESAQQVKLRWLRLEGVTSDLQLLAETDLELVL
EELEALRPAVAIIDSIQALHDGSLSSAPGSVAQVRECAAALQRLAKRQETALVLVGHVTKEGMLAGPKVLEHLVDAVLTF
EGDRFASHRLLRAVKNRFGATHELGVFEMQGKGLAEVGNPSELFLKGESASGVATIVAFEGTRSLVVDLQALVGVTSYAS
PRRTATGLGTNRLHQILAVLEKHMGLPLSRYDCYLAVAGGLEVEEPAADLGVAAAVVSSYRDLMLPKGTVLLGELGLGGQ
LRPVGQLGQRLKEAVRLGFHRAVVPQGSGLGPIGKELELELLEAASVTEALVMALGVNPADDGC

Sequences:

>Translated_464_residues
MRTASVYVCQSCGAKTSQFFGRCASCGTWNSLVEQVAHSTDGRRRSNSFDPAVEPVARRSMAMASLGDQPVRRLASGYEE
LDRVLGGGLVPGSMVLVGGDPGIGKSTLLLQSATAMALQHSVLYVSAEESAQQVKLRWLRLEGVTSDLQLLAETDLELVL
EELEALRPAVAIIDSIQALHDGSLSSAPGSVAQVRECAAALQRLAKRQETALVLVGHVTKEGMLAGPKVLEHLVDAVLTF
EGDRFASHRLLRAVKNRFGATHELGVFEMQGKGLAEVGNPSELFLKGESASGVATIVAFEGTRSLVVDLQALVGVTSYAS
PRRTATGLGTNRLHQILAVLEKHMGLPLSRYDCYLAVAGGLEVEEPAADLGVAAAVVSSYRDLMLPKGTVLLGELGLGGQ
LRPVGQLGQRLKEAVRLGFHRAVVPQGSGLGPIGKELELELLEAASVTEALVMALGVNPADDGC
>Mature_464_residues
MRTASVYVCQSCGAKTSQFFGRCASCGTWNSLVEQVAHSTDGRRRSNSFDPAVEPVARRSMAMASLGDQPVRRLASGYEE
LDRVLGGGLVPGSMVLVGGDPGIGKSTLLLQSATAMALQHSVLYVSAEESAQQVKLRWLRLEGVTSDLQLLAETDLELVL
EELEALRPAVAIIDSIQALHDGSLSSAPGSVAQVRECAAALQRLAKRQETALVLVGHVTKEGMLAGPKVLEHLVDAVLTF
EGDRFASHRLLRAVKNRFGATHELGVFEMQGKGLAEVGNPSELFLKGESASGVATIVAFEGTRSLVVDLQALVGVTSYAS
PRRTATGLGTNRLHQILAVLEKHMGLPLSRYDCYLAVAGGLEVEEPAADLGVAAAVVSSYRDLMLPKGTVLLGELGLGGQ
LRPVGQLGQRLKEAVRLGFHRAVVPQGSGLGPIGKELELELLEAASVTEALVMALGVNPADDGC

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=422, Percent_Identity=46.6824644549763, Blast_Score=368, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 48942; Mature: 48942

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTASVYVCQSCGAKTSQFFGRCASCGTWNSLVEQVAHSTDGRRRSNSFDPAVEPVARRS
CCCCCEEEEHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
MAMASLGDQPVRRLASGYEELDRVLGGGLVPGSMVLVGGDPGIGKSTLLLQSATAMALQH
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHH
SVLYVSAEESAQQVKLRWLRLEGVTSDLQLLAETDLELVLEELEALRPAVAIIDSIQALH
HEEEEECHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DGSLSSAPGSVAQVRECAAALQRLAKRQETALVLVGHVTKEGMLAGPKVLEHLVDAVLTF
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHC
EGDRFASHRLLRAVKNRFGATHELGVFEMQGKGLAEVGNPSELFLKGESASGVATIVAFE
CCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCEEEECCCCCCEEEEEEEC
GTRSLVVDLQALVGVTSYASPRRTATGLGTNRLHQILAVLEKHMGLPLSRYDCYLAVAGG
CCHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCHHCCEEEEEECC
LEVEEPAADLGVAAAVVSSYRDLMLPKGTVLLGELGLGGQLRPVGQLGQRLKEAVRLGFH
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
RAVVPQGSGLGPIGKELELELLEAASVTEALVMALGVNPADDGC
HEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRTASVYVCQSCGAKTSQFFGRCASCGTWNSLVEQVAHSTDGRRRSNSFDPAVEPVARRS
CCCCCEEEEHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
MAMASLGDQPVRRLASGYEELDRVLGGGLVPGSMVLVGGDPGIGKSTLLLQSATAMALQH
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHH
SVLYVSAEESAQQVKLRWLRLEGVTSDLQLLAETDLELVLEELEALRPAVAIIDSIQALH
HEEEEECHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DGSLSSAPGSVAQVRECAAALQRLAKRQETALVLVGHVTKEGMLAGPKVLEHLVDAVLTF
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHC
EGDRFASHRLLRAVKNRFGATHELGVFEMQGKGLAEVGNPSELFLKGESASGVATIVAFE
CCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCEEEECCCCCCEEEEEEEC
GTRSLVVDLQALVGVTSYASPRRTATGLGTNRLHQILAVLEKHMGLPLSRYDCYLAVAGG
CCHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCHHCCEEEEEECC
LEVEEPAADLGVAAAVVSSYRDLMLPKGTVLLGELGLGGQLRPVGQLGQRLKEAVRLGFH
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
RAVVPQGSGLGPIGKELELELLEAASVTEALVMALGVNPADDGC
HEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]