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The map label for this gene is sspA [C]

Identifier: 33864226

GI number: 33864226

Start: 2072098

End: 2072823

Strand: Reverse

Name: sspA [C]

Synonym: PMT1961

Alternate gene names: 33864226

Gene position: 2072823-2072098 (Counterclockwise)

Preceding gene: 33864232

Following gene: 33864225

Centisome position: 85.98

GC content: 53.86

Gene sequence:

>726_bases
ATGATGGAACTGCATCAATTCCGCCATTCCGCCTTCTGTCTCAAGGTGCGGATGGTCTTGCAGGCCAAGGGTTTGACTTA
CCAGGTGGTGGAAGTTACCCCCGGGTTAGGACAGCTCAACGTGTTTCGCTTGTCAGGTCAGCGTCAGGTCCCGGTACTTG
TCGATGGCGATGTTGTTTTGGCAGATTCCAGCACTATTGCTCGACATCTAGAGGCTAAGCAGCCGGAACCACCCCTCATT
CCGGTTGATCCACAGCAGGCCGCGCAGGTGTACTTGATTGAAGATTGGGCTGATACGACATTGGCGCGGGCAGGCCGGTC
AGCTTTGTTGCAGGCTGCTGCCTTGGATTCTGAGTTGCGTTTAGCTCTTCTTCCAGAAGATTTGCCAACTCCCTTGCGTC
AGGTCATGGGAGATCTCCCTGGGGGGTTGTTGAACGGTGCCTCTGAGATCATTGGCAAGGCAGAGAGGGTTGAGTTATTA
ACGAGCCTCGAACAATTGGCTGATTTGATGCAGAAAAGGTCCTGGCTAGTAGGAGATGCGATGAGCTTGGCTGATTTAGC
TGTAGCCGCTCAGATTTCTTTAATGCGTTTCCCAGCCTCGGCTGGGGCAGCACTTGCCGGTAAAGGTGTGTTCGGCCTGA
GTGATCACCCGCGGCTGCAGCCCTTATTCCACTGGAGGGATCAGCTTGAGTTGTCATTGCTTGAGAAGGATGACTCAGCC
CGATGA

Upstream 100 bases:

>100_bases
TTTACTCTCAGCCGAAAGTGAAATGCGATTATTGCCCTCTTTGCCTTAGCGGGCTTTTGACGCTGTTAGCTACTCTGCCG
TTCTTTTGGCAGAGCAAACG

Downstream 100 bases:

>100_bases
GCCTTGCTTGAACATCGATCCAGAAATGGCTGTCTAATGTCTGATCCCTTGATTCGTGATTCTGATCACTACGTCGTTTT
GGAGCCTGGCCAGTCTGAGC

Product: glutathione S-transferase

Products: HX; R-S-glutathione

Alternate protein names: Glutathione S-Transferase Domain-Containing Protein; Glutathione S-Transferase Domain Protein; Glutathione S-Transferase N-Terminal Domain Protein; Glutathione S-Transferase Zeta Class; Glutathione S-Transferase-Like

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MMELHQFRHSAFCLKVRMVLQAKGLTYQVVEVTPGLGQLNVFRLSGQRQVPVLVDGDVVLADSSTIARHLEAKQPEPPLI
PVDPQQAAQVYLIEDWADTTLARAGRSALLQAAALDSELRLALLPEDLPTPLRQVMGDLPGGLLNGASEIIGKAERVELL
TSLEQLADLMQKRSWLVGDAMSLADLAVAAQISLMRFPASAGAALAGKGVFGLSDHPRLQPLFHWRDQLELSLLEKDDSA
R

Sequences:

>Translated_241_residues
MMELHQFRHSAFCLKVRMVLQAKGLTYQVVEVTPGLGQLNVFRLSGQRQVPVLVDGDVVLADSSTIARHLEAKQPEPPLI
PVDPQQAAQVYLIEDWADTTLARAGRSALLQAAALDSELRLALLPEDLPTPLRQVMGDLPGGLLNGASEIIGKAERVELL
TSLEQLADLMQKRSWLVGDAMSLADLAVAAQISLMRFPASAGAALAGKGVFGLSDHPRLQPLFHWRDQLELSLLEKDDSA
R
>Mature_241_residues
MMELHQFRHSAFCLKVRMVLQAKGLTYQVVEVTPGLGQLNVFRLSGQRQVPVLVDGDVVLADSSTIARHLEAKQPEPPLI
PVDPQQAAQVYLIEDWADTTLARAGRSALLQAAALDSELRLALLPEDLPTPLRQVMGDLPGGLLNGASEIIGKAERVELL
TSLEQLADLMQKRSWLVGDAMSLADLAVAAQISLMRFPASAGAALAGKGVFGLSDHPRLQPLFHWRDQLELSLLEKDDSA
R

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.5.1.18

Molecular weight: Translated: 26224; Mature: 26224

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMELHQFRHSAFCLKVRMVLQAKGLTYQVVEVTPGLGQLNVFRLSGQRQVPVLVDGDVVL
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEECCCCCCCEEECCCEEE
ADSSTIARHLEAKQPEPPLIPVDPQQAAQVYLIEDWADTTLARAGRSALLQAAALDSELR
ECCHHHHHHHHCCCCCCCCCCCCHHHHCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCE
LALLPEDLPTPLRQVMGDLPGGLLNGASEIIGKAERVELLTSLEQLADLMQKRSWLVGDA
EEECCCCCCHHHHHHHHHCCCHHHCCHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHCCH
MSLADLAVAAQISLMRFPASAGAALAGKGVFGLSDHPRLQPLFHWRDQLELSLLEKDDSA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHEEEECCCCCC
R
C
>Mature Secondary Structure
MMELHQFRHSAFCLKVRMVLQAKGLTYQVVEVTPGLGQLNVFRLSGQRQVPVLVDGDVVL
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEECCCCCCCEEECCCEEE
ADSSTIARHLEAKQPEPPLIPVDPQQAAQVYLIEDWADTTLARAGRSALLQAAALDSELR
ECCHHHHHHHHCCCCCCCCCCCCHHHHCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCE
LALLPEDLPTPLRQVMGDLPGGLLNGASEIIGKAERVELLTSLEQLADLMQKRSWLVGDA
EEECCCCCCHHHHHHHHHCCCHHHCCHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHCCH
MSLADLAVAAQISLMRFPASAGAALAGKGVFGLSDHPRLQPLFHWRDQLELSLLEKDDSA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHEEEECCCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA