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The map label for this gene is rffG [C]

Identifier: 33864169

GI number: 33864169

Start: 2006310

End: 2007332

Strand: Reverse

Name: rffG [C]

Synonym: PMT1903

Alternate gene names: 33864169

Gene position: 2007332-2006310 (Counterclockwise)

Preceding gene: 33864170

Following gene: 33864166

Centisome position: 83.26

GC content: 52.59

Gene sequence:

>1023_bases
ATGAGTCGATGCGTTGTTGTTACCGGTGCTGCAGGATTCATTGGTGCTGCCCTCGTTCAGAGGCTGTTAGCCCAGGGTGA
CCGTGTCATTGGCATTGACAACCTGAATGACTACTACGATCCGTCTTTGAAGCGGGCACGACTTGCTCAGGTTGAAGCGG
CTTCCCTTAAAGCGTCTTGGACTTTTTATCCAATAGCCTTGGAAGATGGTGCAGCTATAGAGGAACTGTTTAAGGCTGAG
AAGCCTGAGGTGGTCGTTAATTTGGCGGCTCAGGCAGGGGTTCGCTACTCATTGGAGAATCCGGCGGCCTACATTCAGGC
CAATTTGGTGGGCTTCGGTCATATCCTTGAGGGATGTCGGCATCATGGAGTGCAGCACTTGGTGTATGCCTCCAGTAGCT
CGGTATATGGTGGCAATCGCAACTTGCCTTTTCACGAGCAACAGCCTGTGAATCACCCGGTGAGTCTCTATGCAGCCACC
AAGAAGGCCAACGAATTGATGGCTCACACCTATAGCCATTTGTATGGCTTGCCTGCTACAGGATTGCGCTTTTTTACTGT
GTACGGACCTTGGGGCCGCCCGGACATGGCTCCGATGCTCTTCGCCAAGGCGATTCTTGCCGGCGAGCCCATCAAGGTGT
TCAATTACGGCAAGATGCAGCGTGATTTCACTTATATCGACGATATCGTTGAAGGGCTACTTCGGTGTTGCGACAAGCCT
GCTACCGCAAATTTAGGCTTCGATCCGCGGAACCCTGATCCAGCCACCGCTGCTGTGCCCCACCGTCTTTTCAATATTGG
TAATAGCCAGCCCATTGAGTTGATGCATTTTATTGAGTTCTTGGAGAACTCTCTCGGGAGGGAAGCGGTGAAGGACTTCC
AGCCGATGCAACCTGGTGATGTGATGGCCACCGCTGCTGATACATCTGCTCTTGAGGCCTGGGTGGATTTTCGGCCGTCG
ACCCCAATCGCAGAGGGGGTGGAGTGCTTTTCTCAGTGGTATCGAAGCTTTTATGAGGTTTGA

Upstream 100 bases:

>100_bases
TGCGTCAACCTGCCTGGGTGTTTGATGCTCGCGCTGTTGTCGATCCAGAAGTTGTGAAATCGTCAGGTTTGATGCTTTGG
CGAGTTGGAGATGGTGCTGT

Downstream 100 bases:

>100_bases
TCGATAGCTTGATATGCATGTGCATGTAGGCGTCAACGCACCATCGCCGCAACATTTCCGCCGTCTACTGTCAGTAATGC
ACCAGTGGTTCGCGGCATTA

Product: nucleotide sugar epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MSRCVVVTGAAGFIGAALVQRLLAQGDRVIGIDNLNDYYDPSLKRARLAQVEAASLKASWTFYPIALEDGAAIEELFKAE
KPEVVVNLAAQAGVRYSLENPAAYIQANLVGFGHILEGCRHHGVQHLVYASSSSVYGGNRNLPFHEQQPVNHPVSLYAAT
KKANELMAHTYSHLYGLPATGLRFFTVYGPWGRPDMAPMLFAKAILAGEPIKVFNYGKMQRDFTYIDDIVEGLLRCCDKP
ATANLGFDPRNPDPATAAVPHRLFNIGNSQPIELMHFIEFLENSLGREAVKDFQPMQPGDVMATAADTSALEAWVDFRPS
TPIAEGVECFSQWYRSFYEV

Sequences:

>Translated_340_residues
MSRCVVVTGAAGFIGAALVQRLLAQGDRVIGIDNLNDYYDPSLKRARLAQVEAASLKASWTFYPIALEDGAAIEELFKAE
KPEVVVNLAAQAGVRYSLENPAAYIQANLVGFGHILEGCRHHGVQHLVYASSSSVYGGNRNLPFHEQQPVNHPVSLYAAT
KKANELMAHTYSHLYGLPATGLRFFTVYGPWGRPDMAPMLFAKAILAGEPIKVFNYGKMQRDFTYIDDIVEGLLRCCDKP
ATANLGFDPRNPDPATAAVPHRLFNIGNSQPIELMHFIEFLENSLGREAVKDFQPMQPGDVMATAADTSALEAWVDFRPS
TPIAEGVECFSQWYRSFYEV
>Mature_339_residues
SRCVVVTGAAGFIGAALVQRLLAQGDRVIGIDNLNDYYDPSLKRARLAQVEAASLKASWTFYPIALEDGAAIEELFKAEK
PEVVVNLAAQAGVRYSLENPAAYIQANLVGFGHILEGCRHHGVQHLVYASSSSVYGGNRNLPFHEQQPVNHPVSLYAATK
KANELMAHTYSHLYGLPATGLRFFTVYGPWGRPDMAPMLFAKAILAGEPIKVFNYGKMQRDFTYIDDIVEGLLRCCDKPA
TANLGFDPRNPDPATAAVPHRLFNIGNSQPIELMHFIEFLENSLGREAVKDFQPMQPGDVMATAADTSALEAWVDFRPST
PIAEGVECFSQWYRSFYEV

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=345, Percent_Identity=26.0869565217391, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI56237023, Length=359, Percent_Identity=24.5125348189415, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI56118217, Length=359, Percent_Identity=24.5125348189415, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI189083684, Length=359, Percent_Identity=24.5125348189415, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI42516563, Length=345, Percent_Identity=22.6086956521739, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI193211614, Length=300, Percent_Identity=25, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI8393516, Length=300, Percent_Identity=25, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI48994969, Length=350, Percent_Identity=26.2857142857143, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1788353, Length=249, Percent_Identity=27.710843373494, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1786974, Length=347, Percent_Identity=22.478386167147, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1788589, Length=251, Percent_Identity=27.0916334661355, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71982035, Length=354, Percent_Identity=25.1412429378531, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=356, Percent_Identity=25, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17568069, Length=336, Percent_Identity=25.5952380952381, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI115532424, Length=248, Percent_Identity=28.6290322580645, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=250, Percent_Identity=29.2, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19923002, Length=349, Percent_Identity=25.214899713467, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37475; Mature: 37344

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRCVVVTGAAGFIGAALVQRLLAQGDRVIGIDNLNDYYDPSLKRARLAQVEAASLKASW
CCCEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHEEECE
TFYPIALEDGAAIEELFKAEKPEVVVNLAAQAGVRYSLENPAAYIQANLVGFGHILEGCR
EEEEEEECCCHHHHHHHCCCCCCEEEEEHHHCCCEEECCCCCEEEEEHHHHHHHHHHHHH
HHGVQHLVYASSSSVYGGNRNLPFHEQQPVNHPVSLYAATKKANELMAHTYSHLYGLPAT
HCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCC
GLRFFTVYGPWGRPDMAPMLFAKAILAGEPIKVFNYGKMQRDFTYIDDIVEGLLRCCDKP
CEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCC
ATANLGFDPRNPDPATAAVPHRLFNIGNSQPIELMHFIEFLENSLGREAVKDFQPMQPGD
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHCCCCCCCC
VMATAADTSALEAWVDFRPSTPIAEGVECFSQWYRSFYEV
EEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRCVVVTGAAGFIGAALVQRLLAQGDRVIGIDNLNDYYDPSLKRARLAQVEAASLKASW
CCEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHEEECE
TFYPIALEDGAAIEELFKAEKPEVVVNLAAQAGVRYSLENPAAYIQANLVGFGHILEGCR
EEEEEEECCCHHHHHHHCCCCCCEEEEEHHHCCCEEECCCCCEEEEEHHHHHHHHHHHHH
HHGVQHLVYASSSSVYGGNRNLPFHEQQPVNHPVSLYAATKKANELMAHTYSHLYGLPAT
HCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCC
GLRFFTVYGPWGRPDMAPMLFAKAILAGEPIKVFNYGKMQRDFTYIDDIVEGLLRCCDKP
CEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCC
ATANLGFDPRNPDPATAAVPHRLFNIGNSQPIELMHFIEFLENSLGREAVKDFQPMQPGD
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHCCCCCCCC
VMATAADTSALEAWVDFRPSTPIAEGVECFSQWYRSFYEV
EEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]