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The map label for this gene is yuxG [H]

Identifier: 33864168

GI number: 33864168

Start: 2004130

End: 2006277

Strand: Direct

Name: yuxG [H]

Synonym: PMT1902

Alternate gene names: 33864168

Gene position: 2004130-2006277 (Clockwise)

Preceding gene: 33864167

Following gene: 33864173

Centisome position: 83.13

GC content: 53.91

Gene sequence:

>2148_bases
ATGACGTGCCAAAACCGCTGGTCGGATGCCGAGGCACAAGCTGCCATCAAGTCTTACGCCGCGCAGGACGTCTCTGAAGA
CCTGGCTCTCCGCACTTACACAGCCCGTCTGCTCGGCTCAGATCCCCAGCTGGTGCTGCATGGAGGAGGCAACACCTCGG
TTAAAACCAGCTGCATAGGATTGTTTGGCGATCACATACCAGTGTTGTGCGTGAAGGGCTCGGGCTGGGACCTATCAACC
ATCGAGCCGGCCGGCCATCCTGCTGTGCGATTGGAGAACCTGCAGGCCTTAAGAGATCTATCTGCACTTAGCGACGAAGA
CATGGTTGCAGCTCAACGCAGCAACCTGATCGATCCATCGTCACCCAACCCTTCGGTTGAAGCACTCCTACACGCGTTCT
TACCAAGCAAATTCGTCGATCACACCCACGCAGTAGCTGTTTTAGCCCTAGCAGATCAACCAGATGCCCAACAGATCTGC
CGTGAACTCTACGGTCGACGTGTCGCGATTGTTCCCTATGTCATGCCTGGCTTTCAACTAGCCTTAGCCGCCATCAAAGC
CTACGAGCAAGCAGAAGTAGAAGCAGCTCAAGCGGGAGTTGAACTTGAAGGGATGGTGCTTCTCAAGCACGGCTTATTTT
CGTTTGGACCCACAGCACAACAAAGCTACGAGCGAATGATCAACTTGGTGCGTAAGGCGGAAGAGCGTCTTGGGGAAACC
CCAACGCTTTGCCTACCACCACCAACCAATCCAGCTCCTAAAAAAAACATCGCTGCGATTCTGCTCCCACTTTTACGTGG
TGCCCTGGCTCAATCCGCAGCTGTGCATAACGCCCCCCAACGTTGGCTTATGGAGTTGCGCTCAACCCCACTGGCACTCC
AACTAGTAAATGACATTCACCTCCAAGACTGGTCTCGCCGTGGAGTCGCGACCCCCGACCACGTGATCCGAACCAAACCC
TGGCCTCTCATTCTCAAAAAGCCTCCACAACTCCAAGGAGATGAAGCGATTGAATCCTGCCACGTGCTGGAGGAATGGCT
CCACTCAGCAAAACTGGCATTAGAGAAATACATCAACTCATATCAGGATTACTTCGAGCGTCAGAATGCTCGCGTTGGAA
GCCATAAACAACACCTCGATCCACTACCAAGGCTGATCGCAATCCCTGAACTTGGCCTCGTTGGCCTAGGACGTTCAACC
GCCGAAGCCAATGTCACCGCAGATATTGGTGAAGCCTGGGCCGCCACACTGATGGCAGCTGAATCAGTGGGACGTTTTCA
ACCAGTCAACGAGGCAGATACCTTCGAGATGGAGTACTGGAGTCTCGAACAAGCAAAGCTCGGCAAGGGCAAAGAAGCAC
CACTGGCACGCCATGTTGTCTTGGTCACTGGTGGTGGTGGTGGAATTGGTGCAGCGATCGCCCTTGCCTTCGCCAAGCAA
GGTGCACAAGTTGTCGTACTTGACAAGAATGGTGAAGCCGCAACAACGACTGCCAAAGAATGTGGCTCAAGCGCTCTCGG
ACTGAAGTGCGACCTCACCAATGCGTCTGAGGTCCATGATGCATTCACGACAATTGCAGCTTGCTTCGGGGGTTTAGACA
TCGTGGTATCCAATGCTGGGGCGGCTTGGAGCGGAGACATTGCCACTCTTCCAGAATCCAAGTTGCGAGCAAGCTTCGAG
CTCAACCTATTTGCTCACCAGCACGTTGCACAAGCTGCAGTTCGCCTGTTTCGAGCCCAGGGCAATAGAACGACAGAAAC
CAGCAAATCTTTAGGCGGACAGCTGCTCTTCAATGTCAGCAAGCAAGCGCTAAACCCAGGCCCTGGTTTTGGAGCTTACG
GAATTGCAAAAGCTGCATTGCTGGCACTTATGAAGCAATACGCCCTGGAAGAAGGGCCCTCAAGCATTCGCTGTAACGCC
ATCAATGCAGATCGGATTCGGTCCGGCCTGCTCGATCAAGCAATGATTCGAGAACGAGCGGAAGCGCGCGGCATCAGCGA
AGCCAACTACATGGGTGGGAACTTGCTCGGTGCAGAAGTCCGAGCAAGTGATGTGGCAAATGCTTTCGTAGCATTAGCCT
TAATGCCGCGAACCACTGGTGCATTACTGACAGTAGACGGCGGAAATGTTGCGGCGATGGTGCGTTGA

Upstream 100 bases:

>100_bases
AACTGCCTAATAAAGCTTTGAGCCTTAAACATCAGCAAAGGAGAAAGCAGAGGAACTTCAGCGCGACTGAAGTAACGTTC
CCGTCGATACGCACCAATCA

Downstream 100 bases:

>100_bases
CGCCTACATGCACATGCATATCAAGCTATCGATCAAACCTCATAAAAGCTTCGATACCACTGAGAAAAGCACTCCACCCC
CTCTGCGATTGGGGTCGACG

Product: short chain dehydrogenase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 715; Mature: 714

Protein sequence:

>715_residues
MTCQNRWSDAEAQAAIKSYAAQDVSEDLALRTYTARLLGSDPQLVLHGGGNTSVKTSCIGLFGDHIPVLCVKGSGWDLST
IEPAGHPAVRLENLQALRDLSALSDEDMVAAQRSNLIDPSSPNPSVEALLHAFLPSKFVDHTHAVAVLALADQPDAQQIC
RELYGRRVAIVPYVMPGFQLALAAIKAYEQAEVEAAQAGVELEGMVLLKHGLFSFGPTAQQSYERMINLVRKAEERLGET
PTLCLPPPTNPAPKKNIAAILLPLLRGALAQSAAVHNAPQRWLMELRSTPLALQLVNDIHLQDWSRRGVATPDHVIRTKP
WPLILKKPPQLQGDEAIESCHVLEEWLHSAKLALEKYINSYQDYFERQNARVGSHKQHLDPLPRLIAIPELGLVGLGRST
AEANVTADIGEAWAATLMAAESVGRFQPVNEADTFEMEYWSLEQAKLGKGKEAPLARHVVLVTGGGGGIGAAIALAFAKQ
GAQVVVLDKNGEAATTTAKECGSSALGLKCDLTNASEVHDAFTTIAACFGGLDIVVSNAGAAWSGDIATLPESKLRASFE
LNLFAHQHVAQAAVRLFRAQGNRTTETSKSLGGQLLFNVSKQALNPGPGFGAYGIAKAALLALMKQYALEEGPSSIRCNA
INADRIRSGLLDQAMIRERAEARGISEANYMGGNLLGAEVRASDVANAFVALALMPRTTGALLTVDGGNVAAMVR

Sequences:

>Translated_715_residues
MTCQNRWSDAEAQAAIKSYAAQDVSEDLALRTYTARLLGSDPQLVLHGGGNTSVKTSCIGLFGDHIPVLCVKGSGWDLST
IEPAGHPAVRLENLQALRDLSALSDEDMVAAQRSNLIDPSSPNPSVEALLHAFLPSKFVDHTHAVAVLALADQPDAQQIC
RELYGRRVAIVPYVMPGFQLALAAIKAYEQAEVEAAQAGVELEGMVLLKHGLFSFGPTAQQSYERMINLVRKAEERLGET
PTLCLPPPTNPAPKKNIAAILLPLLRGALAQSAAVHNAPQRWLMELRSTPLALQLVNDIHLQDWSRRGVATPDHVIRTKP
WPLILKKPPQLQGDEAIESCHVLEEWLHSAKLALEKYINSYQDYFERQNARVGSHKQHLDPLPRLIAIPELGLVGLGRST
AEANVTADIGEAWAATLMAAESVGRFQPVNEADTFEMEYWSLEQAKLGKGKEAPLARHVVLVTGGGGGIGAAIALAFAKQ
GAQVVVLDKNGEAATTTAKECGSSALGLKCDLTNASEVHDAFTTIAACFGGLDIVVSNAGAAWSGDIATLPESKLRASFE
LNLFAHQHVAQAAVRLFRAQGNRTTETSKSLGGQLLFNVSKQALNPGPGFGAYGIAKAALLALMKQYALEEGPSSIRCNA
INADRIRSGLLDQAMIRERAEARGISEANYMGGNLLGAEVRASDVANAFVALALMPRTTGALLTVDGGNVAAMVR
>Mature_714_residues
TCQNRWSDAEAQAAIKSYAAQDVSEDLALRTYTARLLGSDPQLVLHGGGNTSVKTSCIGLFGDHIPVLCVKGSGWDLSTI
EPAGHPAVRLENLQALRDLSALSDEDMVAAQRSNLIDPSSPNPSVEALLHAFLPSKFVDHTHAVAVLALADQPDAQQICR
ELYGRRVAIVPYVMPGFQLALAAIKAYEQAEVEAAQAGVELEGMVLLKHGLFSFGPTAQQSYERMINLVRKAEERLGETP
TLCLPPPTNPAPKKNIAAILLPLLRGALAQSAAVHNAPQRWLMELRSTPLALQLVNDIHLQDWSRRGVATPDHVIRTKPW
PLILKKPPQLQGDEAIESCHVLEEWLHSAKLALEKYINSYQDYFERQNARVGSHKQHLDPLPRLIAIPELGLVGLGRSTA
EANVTADIGEAWAATLMAAESVGRFQPVNEADTFEMEYWSLEQAKLGKGKEAPLARHVVLVTGGGGGIGAAIALAFAKQG
AQVVVLDKNGEAATTTAKECGSSALGLKCDLTNASEVHDAFTTIAACFGGLDIVVSNAGAAWSGDIATLPESKLRASFEL
NLFAHQHVAQAAVRLFRAQGNRTTETSKSLGGQLLFNVSKQALNPGPGFGAYGIAKAALLALMKQYALEEGPSSIRCNAI
NADRIRSGLLDQAMIRERAEARGISEANYMGGNLLGAEVRASDVANAFVALALMPRTTGALLTVDGGNVAAMVR

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: COG3347

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=214, Percent_Identity=32.7102803738318, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI126723191, Length=215, Percent_Identity=29.7674418604651, Blast_Score=82, Evalue=3e-15,
Organism=Homo sapiens, GI210032110, Length=125, Percent_Identity=29.6, Blast_Score=71, Evalue=4e-12,
Organism=Homo sapiens, GI40254992, Length=252, Percent_Identity=25.3968253968254, Blast_Score=68, Evalue=3e-11,
Organism=Homo sapiens, GI126723750, Length=218, Percent_Identity=29.8165137614679, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI1787905, Length=263, Percent_Identity=30.4182509505703, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1789378, Length=218, Percent_Identity=30.2752293577982, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI1786812, Length=255, Percent_Identity=29.4117647058824, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI87082100, Length=199, Percent_Identity=27.6381909547739, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17560150, Length=271, Percent_Identity=26.9372693726937, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28571526, Length=263, Percent_Identity=28.5171102661597, Blast_Score=80, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21357041, Length=263, Percent_Identity=28.1368821292776, Blast_Score=74, Evalue=5e-13,
Organism=Drosophila melanogaster, GI23397609, Length=268, Percent_Identity=29.8507462686567, Blast_Score=73, Evalue=8e-13,
Organism=Drosophila melanogaster, GI21355319, Length=203, Percent_Identity=30.0492610837438, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24644339, Length=267, Percent_Identity=27.7153558052434, Blast_Score=70, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24640492, Length=143, Percent_Identity=30.0699300699301, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303
- InterPro:   IPR013454
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short; PF00596 Aldolase_II [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 76385; Mature: 76254

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCQNRWSDAEAQAAIKSYAAQDVSEDLALRTYTARLLGSDPQLVLHGGGNTSVKTSCIG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHH
LFGDHIPVLCVKGSGWDLSTIEPAGHPAVRLENLQALRDLSALSDEDMVAAQRSNLIDPS
HHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
SPNPSVEALLHAFLPSKFVDHTHAVAVLALADQPDAQQICRELYGRRVAIVPYVMPGFQL
CCCHHHHHHHHHHHHHHHHCCHHHEEEEEECCCCCHHHHHHHHHCCEEEEEEEECCHHHH
ALAAIKAYEQAEVEAAQAGVELEGMVLLKHGLFSFGPTAQQSYERMINLVRKAEERLGET
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
PTLCLPPPTNPAPKKNIAAILLPLLRGALAQSAAVHNAPQRWLMELRSTPLALQLVNDIH
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEHHHHHH
LQDWSRRGVATPDHVIRTKPWPLILKKPPQLQGDEAIESCHVLEEWLHSAKLALEKYINS
HHHHCCCCCCCCCHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQDYFERQNARVGSHKQHLDPLPRLIAIPELGLVGLGRSTAEANVTADIGEAWAATLMAA
HHHHHHHHCCCCCCCHHHCCHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHH
ESVGRFQPVNEADTFEMEYWSLEQAKLGKGKEAPLARHVVLVTGGGGGIGAAIALAFAKQ
HHHCCCCCCCCCCCEEEHHHCCHHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHC
GAQVVVLDKNGEAATTTAKECGSSALGLKCDLTNASEVHDAFTTIAACFGGLDIVVSNAG
CCEEEEEECCCCCCCCCHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCC
AAWSGDIATLPESKLRASFELNLFAHQHVAQAAVRLFRAQGNRTTETSKSLGGQLLFNVS
CCCCCCCCCCCHHHHCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEECH
KQALNPGPGFGAYGIAKAALLALMKQYALEEGPSSIRCNAINADRIRSGLLDQAMIRERA
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
EARGISEANYMGGNLLGAEVRASDVANAFVALALMPRTTGALLTVDGGNVAAMVR
HHCCCCCHHCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEC
>Mature Secondary Structure 
TCQNRWSDAEAQAAIKSYAAQDVSEDLALRTYTARLLGSDPQLVLHGGGNTSVKTSCIG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHH
LFGDHIPVLCVKGSGWDLSTIEPAGHPAVRLENLQALRDLSALSDEDMVAAQRSNLIDPS
HHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
SPNPSVEALLHAFLPSKFVDHTHAVAVLALADQPDAQQICRELYGRRVAIVPYVMPGFQL
CCCHHHHHHHHHHHHHHHHCCHHHEEEEEECCCCCHHHHHHHHHCCEEEEEEEECCHHHH
ALAAIKAYEQAEVEAAQAGVELEGMVLLKHGLFSFGPTAQQSYERMINLVRKAEERLGET
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
PTLCLPPPTNPAPKKNIAAILLPLLRGALAQSAAVHNAPQRWLMELRSTPLALQLVNDIH
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEHHHHHH
LQDWSRRGVATPDHVIRTKPWPLILKKPPQLQGDEAIESCHVLEEWLHSAKLALEKYINS
HHHHCCCCCCCCCHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQDYFERQNARVGSHKQHLDPLPRLIAIPELGLVGLGRSTAEANVTADIGEAWAATLMAA
HHHHHHHHCCCCCCCHHHCCHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHH
ESVGRFQPVNEADTFEMEYWSLEQAKLGKGKEAPLARHVVLVTGGGGGIGAAIALAFAKQ
HHHCCCCCCCCCCCEEEHHHCCHHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHC
GAQVVVLDKNGEAATTTAKECGSSALGLKCDLTNASEVHDAFTTIAACFGGLDIVVSNAG
CCEEEEEECCCCCCCCCHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCC
AAWSGDIATLPESKLRASFELNLFAHQHVAQAAVRLFRAQGNRTTETSKSLGGQLLFNVS
CCCCCCCCCCCHHHHCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEECH
KQALNPGPGFGAYGIAKAALLALMKQYALEEGPSSIRCNAINADRIRSGLLDQAMIRERA
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
EARGISEANYMGGNLLGAEVRASDVANAFVALALMPRTTGALLTVDGGNVAAMVR
HHCCCCCHHCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 8188684 [H]