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The map label for this gene is ndhK [H]

Identifier: 33864158

GI number: 33864158

Start: 1997077

End: 1997829

Strand: Reverse

Name: ndhK [H]

Synonym: PMT1892

Alternate gene names: 33864158

Gene position: 1997829-1997077 (Counterclockwise)

Preceding gene: 33864159

Following gene: 33864157

Centisome position: 82.87

GC content: 54.18

Gene sequence:

>753_bases
ATGTCAGTGACCCCCTCCATCGATGCCGTACGTGACTTGCGTGCAGCGAGTTGTGGCCCCGTAGGCGCTCCCAGTGTGAC
CAGTGACTTGAGTGAGAACATCATTCTCACAAGCCTGGACGATCTTCACAATTGGGCTCGGCTTAGCAGTCTCTGGCCGT
TGCTGTATGGCACCGCGTGTTGCTTTATCGAGTTCGCGGCTCTGATTGGATCAAGGTTTGATTTTGACCGTTTTGGCTTG
GTGCCCCGAAGCTCTCCGCGGCAGGCTGATCTCCTGATCGTTGCGGGCACGGTGACCATGAAGATGGCTCCAGCCCTGGT
AAGGCTTTATGAGCAGATGCCTGAGCCTAAATACGTGATTGCTATGGGTGCATGCACGATCACTGGTGGCATGTTCAGTG
CTGACTCCACTACCGCTGTGCGTGGAGTAGACAAGCTCATCCCCGTAGATCTCTATCTCCCAGGGTGCCCACCTCGCCCT
GAAGCAATTTTTGATGCGGTGATCAAGTTACGCAAAAAAGTGGGTGATGAATCGGTTGCTGAGCGGATCAAGATTGCCCA
GACCCACCGCTACTTCACAGTGCCCCATCATATGAAGCGAGTTGAGCCAATTGTCACCGGGGCCTACTTGAGTGCTGATA
CCCAGAAAGCTGCCTTGAAGCCTGGTGCTGGGCTACCAATGGCTGCGGAGTTGAACACCCCTGAAATTGATGTCAGCCCA
GCTTCTCAGTCTTCATCAACCTACGAGTCATGA

Upstream 100 bases:

>100_bases
GGTCTATTGGCTTTCATCGAAGCCCTTATTTTTATTGCCATCCTTCTTGTTGCACTGGCTTATGCCTGGCGCAAGGGTGC
CCTCGAGTGGAGTTGATTTC

Downstream 100 bases:

>100_bases
GCGAGACCCCTTCCACGCCCCCGGTGCCGGCGGGTCCGGAACCGGGACCTGTAAGCCGCTGGCTCAACCAGCAGGGTTTT
GAGCATCAGTTGCTTGATCC

Product: NADH dehydrogenase subunit B

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit K; NDH-1 subunit K; NDH-K [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MSVTPSIDAVRDLRAASCGPVGAPSVTSDLSENIILTSLDDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGL
VPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRP
EAIFDAVIKLRKKVGDESVAERIKIAQTHRYFTVPHHMKRVEPIVTGAYLSADTQKAALKPGAGLPMAAELNTPEIDVSP
ASQSSSTYES

Sequences:

>Translated_250_residues
MSVTPSIDAVRDLRAASCGPVGAPSVTSDLSENIILTSLDDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGL
VPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRP
EAIFDAVIKLRKKVGDESVAERIKIAQTHRYFTVPHHMKRVEPIVTGAYLSADTQKAALKPGAGLPMAAELNTPEIDVSP
ASQSSSTYES
>Mature_249_residues
SVTPSIDAVRDLRAASCGPVGAPSVTSDLSENIILTSLDDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGLV
PRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRPE
AIFDAVIKLRKKVGDESVAERIKIAQTHRYFTVPHHMKRVEPIVTGAYLSADTQKAALKPGAGLPMAAELNTPEIDVSPA
SQSSSTYES

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=151, Percent_Identity=50.9933774834437, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI1788624, Length=153, Percent_Identity=49.0196078431373, Blast_Score=168, Evalue=3e-43,
Organism=Escherichia coli, GI1789074, Length=108, Percent_Identity=42.5925925925926, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788834, Length=106, Percent_Identity=42.4528301886792, Blast_Score=90, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=160, Percent_Identity=48.125, Blast_Score=166, Evalue=9e-42,
Organism=Drosophila melanogaster, GI18859983, Length=139, Percent_Identity=55.3956834532374, Blast_Score=174, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24642371, Length=139, Percent_Identity=55.3956834532374, Blast_Score=174, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24651058, Length=136, Percent_Identity=55.1470588235294, Blast_Score=170, Evalue=9e-43,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 26927; Mature: 26796

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTPSIDAVRDLRAASCGPVGAPSVTSDLSENIILTSLDDLHNWARLSSLWPLLYGTAC
CCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
CFIEFAALIGSRFDFDRFGLVPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVI
HHHHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEE
AMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRPEAIFDAVIKLRKKVGDESVA
EECCEEEECCCCCCCCCHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHH
ERIKIAQTHRYFTVPHHMKRVEPIVTGAYLSADTQKAALKPGAGLPMAAELNTPEIDVSP
HHHHHHHHCCEEECCHHHHHHHHHHHHHEECCCCHHHHCCCCCCCCEEEECCCCCEECCC
ASQSSSTYES
CCCCCCCCCC
>Mature Secondary Structure 
SVTPSIDAVRDLRAASCGPVGAPSVTSDLSENIILTSLDDLHNWARLSSLWPLLYGTAC
CCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
CFIEFAALIGSRFDFDRFGLVPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVI
HHHHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEE
AMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRPEAIFDAVIKLRKKVGDESVA
EECCEEEECCCCCCCCCHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHH
ERIKIAQTHRYFTVPHHMKRVEPIVTGAYLSADTQKAALKPGAGLPMAAELNTPEIDVSP
HHHHHHHHCCEEECCHHHHHHHHHHHHHEECCCCHHHHCCCCCCCCEEEECCCCCEECCC
ASQSSSTYES
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA